Not a confocal question

[Matthew Pearson-3]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
This is not specifically a confocal question but a microscopy question none the less.  We have a Zeiss axiovert 100M using a Hamamatsu Orca camera.  There seems to be an image artifact somewhere in the optical train.  When I have the live camera feed there are lots of small circles with smaller rings inside.  Could this be dust that has collected somewhere? I have tried cleaning every surface I can get access to but it is still there! I get it whether doing bright field or fluorescence and it is not dirty objective lenses.  It is not filters within the condenser either as the same particles appear in the image irrespective of the condenser position.  I have removed the camera and cleaned it, still no look.  So I'm at a loose end really as to what it can be.  I can only think it is something within the microscope body beneath the objective housing and I don't really want to dismantle the whole thing! Has anyone had similar problems in the past?

Thanks,

Matt Pearson.

[Julio Vazquez]

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This might be due to dust on the CCD (or the glass window in front of the CCD)

Julio.

On Mar 12, 2008, at 9:29 AM, Matthew Pearson wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
This is not specifically a confocal question but a microscopy question none the less.  We have a Zeiss axiovert 100M using a Hamamatsu Orca camera.  There seems to be an image artifact somewhere in the optical train.  When I have the live camera feed there are lots of small circles with smaller rings inside.  Could this be dust that has collected somewhere? I have tried cleaning every surface I can get access to but it is still there! I get it whether doing bright field or fluorescence and it is not dirty objective lenses.  It is not filters within the condenser either as the same particles appear in the image irrespective of the condenser position.  I have removed the camera and cleaned it, still no look.  So I'm at a loose end really as to what it can be.  I can only think it is something within the microscope body beneath the objective housing and I don't really want to dismantle the whole thing! Has anyone had similar problems in the past?

Thanks,

Matt Pearson.

[Michael Weber-4]

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Matt,

this might be dust on either the camera or the tube lens. What happens
if you turn the camera while imaging? Do the particles move or not?

Michael


Matthew Pearson wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> This is not specifically a confocal question but a microscopy question
> none the less.  We have a Zeiss axiovert 100M using a Hamamatsu Orca
> camera.  There seems to be an image artifact somewhere in the optical
> train.  When I have the live camera feed there are lots of small circles
> with smaller rings inside.  Could this be dust that has collected
> somewhere? I have tried cleaning every surface I can get access to but
> it is still there! I get it whether doing bright field or fluorescence
> and it is not dirty objective lenses.  It is not filters within the
> condenser either as the same particles appear in the image irrespective
> of the condenser position.  I have removed the camera and cleaned it,
> still no look.  So I'm at a loose end really as to what it can be.  I
> can only think it is something within the microscope body beneath the
> objective housing and I don't really want to dismantle the whole thing!
> Has anyone had similar problems in the past?
>
> Thanks,
>
> Matt Pearson.
>
>
> --
> Imaging Technician
> Cell Biology Division
> Institute of Ophthalmology
> University College London
> EC1V 9EL
> 020 7608 6857
> [hidden email]

[Russell Spear]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi

Can you rotate the camera?  If the "dirt" moves it with the camera or
the mount.  I it stays in same place it's somewhere in the microscope
in both bright filed and epi-fluorescence it's got to be objectives or
somewhere above.

Good luck

Russ

Russell N. Spear
Sr. Research Specialist
Dept. of Plant Pathology
Univ. of Wisconsin
1630 Linden Dr.
Madison WI 53706

voice 608.263.2093
fax     [phone]

>>> Matthew Pearson <[hidden email]> 03/12/08 10:29 AM >>>
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal 
This is not specifically a confocal question but a microscopy question
none the less.  We have a Zeiss axiovert 100M using a Hamamatsu Orca
camera.  There seems to be an image artifact somewhere in the optical
train.  When I have the live camera feed there are lots of small circles
with smaller rings inside.  Could this be dust that has collected
somewhere? I have tried cleaning every surface I can get access to but
it is still there! I get it whether doing bright field or fluorescence
and it is not dirty objective lenses.  It is not filters within the
condenser either as the same particles appear in the image irrespective
of the condenser position.  I have removed the camera and cleaned it,
still no look.  So I'm at a loose end really as to what it can be.  I
can only think it is something within the microscope body beneath the
objective housing and I don't really want to dismantle the whole thing!
Has anyone had similar problems in the past?

Thanks,

Matt Pearson.


-- Imaging TechnicianCell Biology DivisionInstitute of
OphthalmologyUniversity College LondonEC1V 9EL020 7608
[hidden email]

[Weis, Michael]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Narrow down the location of the artefacts. Obtain a live image and rotate your Orca at the C-Mount (where it attaches to the microscope). If the artefacts do not move with the image then there is something on the Orca’s sensor. If the artefacts move with the movement of your sample then the artefacts are somewhere in the microscope.

 

Michael Weis

Electron Microscopy & Digital Imaging

Pacific Agri-Food Research Centre

Agriculture and Agri-Food Canada

4200 Highway 97

Summerland, BC  V0H 1Z0

Telephone: [phone]

Facsimile: 250-494-0755

[hidden email]

 

 

P Before printing think about the environment. / Avant d'imprimer, il faut penser à l'environnement.

 

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Matthew Pearson
Sent: Wednesday, March 12, 2008 9:29 AM
To: [hidden email]
Subject: Not a confocal question

 

This is not specifically a confocal question but a microscopy question none the less.  We have a Zeiss axiovert 100M using a Hamamatsu Orca camera.  There seems to be an image artifact somewhere in the optical train.  When I have the live camera feed there are lots of small circles with smaller rings inside.  Could this be dust that has collected somewhere? I have tried cleaning every surface I can get access to but it is still there! I get it whether doing bright field or fluorescence and it is not dirty objective lenses.  It is not filters within the condenser either as the same particles appear in the image irrespective of the condenser position.  I have removed the camera and cleaned it, still no look.  So I'm at a loose end really as to what it can be.  I can only think it is something within the microscope body beneath the objective housing and I don't really want to dismantle the whole thing! Has anyone had similar problems in the past?

Thanks,

Matt Pearson.

 

-- 

Imaging Technician

Cell Biology Division

Institute of Ophthalmology

University College London

EC1V 9EL

020 7608 6857

[hidden email]

[Ian Dobbie-2]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Russell Spear <[hidden email]> writes:

> Can you rotate the camera?  If the "dirt" moves it with the camera or
> the mount.  I it stays in same place it's somewhere in the microscope
> in both bright filed and epi-fluorescence it's got to be objectives or
> somewhere above.

Be careful how you interpret this. I'm sure that Russell knows exactly
what he means, but unless you already know what he is saying it is
difficult to understand. Here is my expanded explanation.

1) If the artifacts are in the same position in each image frame as you
rotate the camera, then it is on the camera, or maybe on its mount.

2) If the artifacts rotate on the image as you rotate the camera then
they are somewhere earlier in the optical setup, probably the tube
lens, or a mirror in the camera body.

If they are nearly in focus (it sounds as though they are) then I
suspect they are on the front window of the CCD. These are not trivial
to clean!

Ian

[Rittling, Susan]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

We had the same frustrating experience with an AxioCam HrC camera. I
tried to clean everything and concluded that the problem was inside the
camera. I finally contacted the Zeiss sales rep, and he replied that
many of these cameras have this problem (maybe a manufacturing defect)
and he replaced it for free.
Maybe it it's the same problem with your camera?  Try contacting your
sales rep.

Susan R Rittling
The Forsyth Institute

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Matthew Pearson
Sent: Wednesday, March 12, 2008 12:29 PM
To: [hidden email]
Subject: Not a confocal question

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
<!DOCTYPE html PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN">
<html>
<head>
</head>
<body bgcolor="#ffffff" text="#000000">
<br>
This is not specifically a confocal question but a microscopy question
none the less.&nbsp; We have a Zeiss axiovert 100M using a Hamamatsu
Orca
camera.&nbsp; There seems to be an image artifact somewhere in the
optical
train.&nbsp; When I have the live camera feed there are lots of small
circles with smaller rings inside.&nbsp; Could this be dust that has
collected somewhere? I have tried cleaning every surface I can get
access to but it is still there! I get it whether doing bright field or
fluorescence and it is not dirty objective lenses.&nbsp; It is not
filters
within the condenser either as the same particles appear in the image
irrespective of the condenser position.&nbsp; I have removed the camera
and
cleaned it, still no look.&nbsp; So I'm at a loose end really as to what
it
can be.&nbsp; I can only think it is something within the microscope
body
beneath the objective housing and I don't really want to dismantle the
whole thing! Has anyone had similar problems in the past? <br>
<br>
Thanks,<br>
<br>
Matt Pearson.<br>
<div class="moz-signature">
<title></title>
<br>
<div class="moz-signature">
<div class="moz-signature"><br>
<pre class="moz-signature" cols="72"><font color="#3366ff"><font
 color="#3333ff"><font face="Arial">--
Imaging Technician
Cell Biology Division
Institute of Ophthalmology
University College London
EC1V 9EL
020 7608 6857
<a class="moz-txt-link-abbreviated"
href="mailto:[hidden email]">[hidden email]</a></f
ont></font><font color="#3333ff">
</font>
</font></pre>
</div>
</div>
</div>
</body>
</html>

[Bowman, Doug]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

The dust could also be on the inside front service of the CCD window,
meaning that you won't be able to clean it.  It would need to go back to
Hamamatsu.

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Ian Dobbie
Sent: Wednesday, March 12, 2008 2:10 PM
To: [hidden email]
Subject: Re: Not a confocal question

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Russell Spear <[hidden email]> writes:

> Can you rotate the camera?  If the "dirt" moves it with the camera or
> the mount.  I it stays in same place it's somewhere in the microscope
> in both bright filed and epi-fluorescence it's got to be objectives or
> somewhere above.

Be careful how you interpret this. I'm sure that Russell knows exactly
what he means, but unless you already know what he is saying it is
difficult to understand. Here is my expanded explanation.

1) If the artifacts are in the same position in each image frame as you
rotate the camera, then it is on the camera, or maybe on its mount.

2) If the artifacts rotate on the image as you rotate the camera then
they are somewhere earlier in the optical setup, probably the tube
lens, or a mirror in the camera body.

If they are nearly in focus (it sounds as though they are) then I
suspect they are on the front window of the CCD. These are not trivial
to clean!

Ian



This e-mail, including any attachments, is a confidential business communication, and may contain information that is confidential, proprietary and/or privileged.  This e-mail is intended only for the individual(s) to whom it is addressed, and may not be saved, copied, printed, disclosed or used by anyone else.  If you are not the(an) intended recipient, please immediately delete this e-mail from your computer system and notify the sender.  Thank you.

[Mayandi Sivaguru]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Matt, we had same problems in two IX-70s one is with Photometrics Sensys camera and other one is coupled with a Trans light PMT detector in a Biorad MRC 2000 confocal system!. I remember we did exactly all those tests suggested by others in this thread and figured out in both cases it is on the detector side.
Shiv


At 01:12 PM 3/12/2008, you wrote:

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

We had the same frustrating experience with an AxioCam HrC camera. I
tried to clean everything and concluded that the problem was inside the
camera. I finally contacted the Zeiss sales rep, and he replied that
many of these cameras have this problem (maybe a manufacturing defect)
and he replaced it for free.
Maybe it it's the same problem with your camera?  Try contacting your
sales rep.

Susan R Rittling
The Forsyth Institute

-----Original Message-----
From: Confocal Microscopy List [[hidden email]] On
Behalf Of Matthew Pearson
Sent: Wednesday, March 12, 2008 12:29 PM
To: [hidden email]
Subject: Not a confocal question

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
<!DOCTYPE html PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN">

This is not specifically a confocal question but a microscopy question none the less.  We have a Zeiss axiovert 100M using a Hamamatsu Orca camera.  There seems to be an image artifact somewhere in the optical train.  When I have the live camera feed there are lots of small circles with smaller rings inside.  Could this be dust that has collected somewhere? I have tried cleaning every surface I can get access to but it is still there! I get it whether doing bright field or fluorescence and it is not dirty objective lenses.  It is not filters within the condenser either as the same particles appear in the image irrespective of the condenser position.  I have removed the camera and cleaned it, still no look.  So I'm at a loose end really as to what it can be.  I can only think it is something within the microscope body beneath the objective housing and I don't really want to dismantle the whole thing! Has anyone had similar problems in the past?

Thanks,

Matt Pearson.

-- 
Imaging Technician
Cell Biology Division
Institute of Ophthalmology
University College London
EC1V 9EL
020 7608 6857
[hidden email]

Mayandi Sivaguru, PhD, PhDMicroscopy Facility Manager
8, Institute for Genomic Biology
University of Illinois at Urbana-Champaign
1206 West Gregory Dr.
Urbana, IL 61801 USA

Office: 217.333.1214
Fax: [phone]
[hidden email]
http://core.igb.uiuc.edu

[Christian Müller-11]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

> When I have the live camera feed there are lots of small
> circles with smaller rings inside.

We experienced apparently similar 'artefacts' in a Spot-camera about
once every 2 years due to humidity accumulation in the camera.
After sending it back for maintenance and paying a considerable amount
of money several times, I successfully did the following some months ago
when the problem reoccurred: i) open the cover of the camera, ii) find
the big screw(s) which close the container for the desiccating material
and open them (see important hint below!), iii) put the sac with
dessicating material in the oven for 5h at 100degC, iv) flush the camera
with dried nitrogen for 1h via the opening, v) reassemble everything  
(using Teflon-tape for the screws).
Camera works nicely ever since. Maybe others have a similar problem with
their cooled CCD's.

Important Hint: be extremely cautious when opening the camera and
removing the desiccating-sac and the old sealing material from the
threads (!). If some material or dust falls into the camera and gets
onto the chip-surface you're in trouble!

Christian M. Müller
JWG University Frankfurt

[COETZEE, S.H. (MR.)]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Not sure if this will help but had a problem with a ghost image that turned out to be a messed up calibration. Just do a Black and shading reference.  If the problem persist, I agree, contact
Zeiss.

Stephan
EMU
University of Botswana
Gaborone
Botswana


-----Original Message-----
From: Confocal Microscopy List on behalf of Mayandi Sivaguru
Sent: Wed 3/12/2008 9:54 PM
To: [hidden email]
Subject: Re: Not a confocal question

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal


Matt, we had same problems in two IX-70s one is with Photometrics
Sensys camera and other one is coupled with a Trans light PMT
detector in a Biorad MRC 2000 confocal system!. I remember we did
exactly all those tests suggested by others in this thread and
figured out in both cases it is on the detector side.
Shiv


At 01:12 PM 3/12/2008, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>We had the same frustrating experience with an AxioCam HrC camera. I
>tried to clean everything and concluded that the problem was inside the
>camera. I finally contacted the Zeiss sales rep, and he replied that
>many of these cameras have this problem (maybe a manufacturing defect)
>and he replaced it for free.
>Maybe it it's the same problem with your camera?  Try contacting your
>sales rep.
>
>Susan R Rittling
>The Forsyth Institute
>
>-----Original Message-----
>From: Confocal Microscopy List [[hidden email]] On
>Behalf Of Matthew Pearson
>Sent: Wednesday, March 12, 2008 12:29 PM
>To: [hidden email]
>Subject: Not a confocal question
>
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
><!DOCTYPE html PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN">
>
>This is not specifically a confocal question but a microscopy
>question none the less.  We have a Zeiss axiovert 100M using a
>Hamamatsu Orca camera.  There seems to be an image artifact
>somewhere in the optical train.  When I have the live camera feed
>there are lots of small circles with smaller rings inside.  Could
>this be dust that has collected somewhere? I have tried cleaning
>every surface I can get access to but it is still there! I get it
>whether doing bright field or fluorescence and it is not dirty
>objective lenses.  It is not filters within the condenser either as
>the same particles appear in the image irrespective of the condenser
>position.  I have removed the camera and cleaned it, still no
>look.  So I'm at a loose end really as to what it can be.  I can
>only think it is something within the microscope body beneath the
>objective housing and I don't really want to dismantle the whole
>thing! Has anyone had similar problems in the past?
>
>Thanks,
>
>Matt Pearson.
>
>
>
>--
>Imaging Technician
>Cell Biology Division
>Institute of Ophthalmology
>University College London
>EC1V 9EL
>020 7608 6857
><[hidden email]>[hidden email]
>
>

Mayandi Sivaguru, PhD, PhDMicroscopy Facility Manager
8, Institute for Genomic Biology
University of Illinois at Urbana-Champaign
1206 West Gregory Dr.
Urbana, IL 61801 USA

Office: 217.333.1214
Fax: [phone]
[hidden email]
http://core.igb.uiuc.edu

[Ian Dobbie-2]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Christian Müller <[hidden email]> writes:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>> When I have the live camera feed there are lots of small
>> circles with smaller rings inside.
>
> We experienced apparently similar 'artefacts' in a Spot-camera about
> once every 2 years due to humidity accumulation in the camera.

[snip]

This is a very useful tip in general but as far as I am aware all the
Hamamatsu cameras now have a vacuum sealed head and so should not have
this problem.

Ian

[Deanne Veronica Catmull]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi!

 

There was someone speaking about a similar issue with stripes and circles as imaging “artifacts” recently and they mentioned that condensation might also be an issue.

 

Kind regards,

Deanne.

 

Deanne Catmull  
Research Assistant
School of Dental Science
University of Melbourne
720 Swanston St
Carlton 3010
Fax: [phone]
Ph: 9341-1577

---

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Mayandi Sivaguru
Sent: Thursday, March 13, 2008 6:54 AM
To: [hidden email]
Subject: Re: Not a confocal question

 

Matt, we had same problems in two IX-70s one is with Photometrics Sensys camera and other one is coupled with a Trans light PMT detector in a Biorad MRC 2000 confocal system!. I remember we did exactly all those tests suggested by others in this thread and figured out in both cases it is on the detector side.
Shiv


At 01:12 PM 3/12/2008, you wrote:

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

We had the same frustrating experience with an AxioCam HrC camera. I
tried to clean everything and concluded that the problem was inside the
camera. I finally contacted the Zeiss sales rep, and he replied that
many of these cameras have this problem (maybe a manufacturing defect)
and he replaced it for free.
Maybe it it's the same problem with your camera?  Try contacting your
sales rep.

Susan R Rittling
The Forsyth Institute

-----Original Message-----
From: Confocal Microscopy List [[hidden email]] On
Behalf Of Matthew Pearson
Sent: Wednesday, March 12, 2008 12:29 PM
To: [hidden email]
Subject: Not a confocal question

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
<!DOCTYPE html PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN">

This is not specifically a confocal question but a microscopy question none the less.  We have a Zeiss axiovert 100M using a Hamamatsu Orca camera.  There seems to be an image artifact somewhere in the optical train.  When I have the live camera feed there are lots of small circles with smaller rings inside.  Could this be dust that has collected somewhere? I have tried cleaning every surface I can get access to but it is still there! I get it whether doing bright field or fluorescence and it is not dirty objective lenses.  It is not filters within the condenser either as the same particles appear in the image irrespective of the condenser position.  I have removed the camera and cleaned it, still no look.  So I'm at a loose end really as to what it can be.  I can only think it is something within the microscope body beneath the objective housing and I don't really want to dismantle the whole thing! Has anyone had similar problems in the past?

Thanks,

Matt Pearson.

-- 

Imaging Technician

Cell Biology Division

Institute of Ophthalmology

University College London

EC1V 9EL

020 7608 6857

[hidden email]

Mayandi Sivaguru, PhD, PhDMicroscopy Facility Manager
8, Institute for Genomic Biology
University of Illinois at Urbana-Champaign
1206 West Gregory Dr.
Urbana, IL 61801 USA

Office: 217.333.1214
Fax: [phone]
[hidden email]
http://core.igb.uiuc.edu

[Beat Ludin]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

I also suspect that what you see is probably condensation on the
cooled CCD.  If you want to test for it without opening the camera,
simply switch the camera off for a day or two and then take pictures
immediately after switching it on again. If the pattern is gone and
then returns as the CCD starts to cool you know for sure what you got.

Beat

At 17:29 12-03-2008, you wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>This is not specifically a confocal question but a microscopy
>question none the less.  We have a Zeiss axiovert 100M using a
>Hamamatsu Orca camera.  There seems to be an image artifact
>somewhere in the optical train.  When I have the live camera feed
>there are lots of small circles with smaller rings inside.  Could
>this be dust that has collected somewhere? I have tried cleaning
>every surface I can get access to but it is still there! I get it
>whether doing bright field or fluorescence and it is not dirty
>objective lenses.  It is not filters within the condenser either as
>the same particles appear in the image irrespective of the condenser
>position.  I have removed the camera and cleaned it, still no
>look.  So I'm at a loose end really as to what it can be.  I can
>only think it is something within the microscope body beneath the
>objective housing and I don't really want to dismantle the whole
>thing! Has anyone had similar problems in the past?
>
>Thanks,
>
>Matt Pearson.
>
>
>
>
>--
>Imaging Technician
>Cell Biology Division
>Institute of Ophthalmology
>University College London
>EC1V 9EL
>020 7608 6857
><mailto:[hidden email]>[hidden email]
>

[Barbara Foster]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Just a reminder that diffraction is active in this sort of imaging and it is always true that light, bending around the edges of very small features, will undergo diffraction and, subsequently, interference.  It is that alternating pattern of constructive destructive interference that causes the finges or bullseye type of patterns.  These are, indeed, artifacts, and the cleaner you can keep your optical system, the less you will see them.

Good hunting!

Barbara Foster, President

Microscopy/Microscopy Education
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McKinney TX 75070
P: [phone]
Skype: fostermme
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MME is now scheduling customized, on-site courses through July 2008.  Call us today for details.



At 01:11 AM 3/26/2008, you wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hi!
 
There was someone speaking about a similar issue with stripes and circles as imaging “artifacts” recently and they mentioned that condensation might also be an issue.
 
Kind regards,
Deanne.
 
Deanne Catmull  Research Assistant
School of Dental Science
University of Melbourne
720 Swanston St
Carlton 3010
Fax: [phone]
Ph: 9341-1577

---

From: Confocal Microscopy List [[hidden email]] On Behalf Of Mayandi Sivaguru
Sent: Thursday, March 13, 2008 6:54 AM
To: [hidden email]
Subject: Re: Not a confocal question
 

Matt, we had same problems in two IX-70s one is with Photometrics Sensys camera and other one is coupled with a Trans light PMT detector in a Biorad MRC 2000 confocal system!. I remember we did exactly all those tests suggested by others in this thread and figured out in both cases it is on the detector side.
Shiv


At 01:12 PM 3/12/2008, you wrote:

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We had the same frustrating experience with an AxioCam HrC camera. I
tried to clean everything and concluded that the problem was inside the
camera. I finally contacted the Zeiss sales rep, and he replied that
many of these cameras have this problem (maybe a manufacturing defect)
and he replaced it for free.
Maybe it it's the same problem with your camera?  Try contacting your
sales rep.

Susan R Rittling
The Forsyth Institute

-----Original Message-----
From: Confocal Microscopy List [ [hidden email]] On
Behalf Of Matthew Pearson
Sent: Wednesday, March 12, 2008 12:29 PM
To: [hidden email]
Subject: Not a confocal question

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This is not specifically a confocal question but a microscopy question none the less.  We have a Zeiss axiovert 100M using a Hamamatsu Orca camera.  There seems to be an image artifact somewhere in the optical train.  When I have the live camera feed there are lots of small circles with smaller rings inside.  Could this be dust that has collected somewhere? I have tried cleaning every surface I can get access to but it is still there! I get it whether doing bright field or fluorescence and it is not dirty objective lenses.  It is not filters within the condenser either as the same particles appear in the image irrespective of the condenser position.  I have removed the camera and cleaned it, still no look.  So I'm at a loose end really as to what it can be.  I can only think it is something within the microscope body beneath the objective housing and I don't really want to dismantle the whole thing! Has anyone had similar problems in the past?

Thanks,

Matt Pearson.

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