Not a confocal question

[vaishali kailaje]

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Dear All
We are working with B16F10 melanoma cell line  which
is a highly adhesive line , recently we are facing the
problem of growing them . When we grow them in petri
plates it spreads very well but when seeded on
coverslips it remains rounded. What could be the
possible reason.
Regards
Vaishali


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[Csucs Gabor]

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Dear Vaishali,

I'd suggest to check whether your slides  are hydrophobic or
hydrophylic. Cells usually don't like hydrophobic ("dirty") glass
slides. A short (oxygen) plasma treatment could help. Of course there
are also many solvent based cleaning protocols around.

Cheers    Gabor

--
Gabor Csucs
Light Microscopy Centre, ETH Zurich
Schafmattstrasse 18, HPM F16
CH-8093, Zurich, Switzerland

Web: www.lmc.ethz.ch
Phone: [phone]
Fax: [phone]
e-mail: [hidden email]

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Hi, Vaishali-
One of my students found that all of our tumor lines (from rat
respiratory tract) rounded up on glass.  The normal lines didn't seem
to care whether they were on glass or plastic.  At least they looked
the same - we use a coated coverslip now.  I vacuum deposit a metal
(platinum is good, germanium, or silicon itself seem to work about as
well).  Then, the cells treat the surface just like tissue culture
plastic.
C.

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Dear All
>We are working with B16F10 melanoma cell line  which
>is a highly adhesive line , recently we are facing the
>problem of growing them . When we grow them in petri
>plates it spreads very well but when seeded on
>coverslips it remains rounded. What could be the
>possible reason.
>Regards
>Vaishali
>
>
>
>____________________________________________________________________________________
>You rock. That's why Blockbuster's offering you one month of
>Blockbuster Total Access, No Cost.
>http://tc.deals.yahoo.com/tc/blockbuster/text5.com

--
Carol A. Heckman, Ph.D.
Director, Center for Microscopy & Microanalysis
and Professor of Biological Sciences
Bowling Green State University
Bowling Green, OH 43403
USA
website: http://www.bgsu.edu/departments/biology/facilities/MnM

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BTW, if you are using an upright microscope, you can grow the cells
on LabTek culture-well slides.  Get the RS, which has a coating that
resembles tissue culture plastic.
Carol

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Dear All
>We are working with B16F10 melanoma cell line  which
>is a highly adhesive line , recently we are facing the
>problem of growing them . When we grow them in petri
>plates it spreads very well but when seeded on
>coverslips it remains rounded. What could be the
>possible reason.
>Regards
>Vaishali
>
>
>
>____________________________________________________________________________________
>You rock. That's why Blockbuster's offering you one month of
>Blockbuster Total Access, No Cost.
>http://tc.deals.yahoo.com/tc/blockbuster/text5.com

--
Carol A. Heckman, Ph.D.
Director, Center for Microscopy & Microanalysis
and Professor of Biological Sciences
Bowling Green State University
Bowling Green, OH 43403
USA
website: http://www.bgsu.edu/departments/biology/facilities/MnM

[Monique Vasseur]

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They probably don't like to grow on glass and prefer plastic.  You could try different adherence coatings to be able to grow them on glass.

Monique Vasseur
Microscopie et imagerie
Département de biochimie
Université de Montréal
C.P. 6128, succursale Centre-ville
Montréal QC    H3C 3J7   Canada
tél. [phone] poste 5148
-----Message d'origine-----
De : Confocal Microscopy List [mailto:[hidden email]] De la part de vaishali kailaje
Envoyé : 3 avril 2008 06:19
À : [hidden email]
Objet : Not a confocal question

Search the CONFOCAL archive at
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Dear All
We are working with B16F10 melanoma cell line  which
is a highly adhesive line , recently we are facing the
problem of growing them . When we grow them in petri
plates it spreads very well but when seeded on
coverslips it remains rounded. What could be the
possible reason.
Regards
Vaishali


      ____________________________________________________________________________________
You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost.  
http://tc.deals.yahoo.com/tc/blockbuster/text5.com

[Begley, James G]

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This is a very common problem with cells growing in culture.  Usually, coating the cover slips with a substrate like poly-D-lysine or collagen will take care of the problem, but there are some cell lines that will never grow as well on glass as plastic.

Jim Begley
Research Facility Manager
Imaging Facility
BBSRB Room 074
741 South Limestone Street
University of Kentucky
Lexington, KY 40536-0509
Phone: [phone]
Fax: [phone]
E-mail: [hidden email]

Other Contact:
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-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of vaishali kailaje
Sent: Thursday, April 03, 2008 6:19 AM
To: [hidden email]
Subject: Not a confocal question

Search the CONFOCAL archive at
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Dear All
We are working with B16F10 melanoma cell line  which
is a highly adhesive line , recently we are facing the
problem of growing them . When we grow them in petri
plates it spreads very well but when seeded on
coverslips it remains rounded. What could be the
possible reason.
Regards
Vaishali


      ____________________________________________________________________________________
You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost.
http://tc.deals.yahoo.com/tc/blockbuster/text5.com