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Not confocal question: Laser Shielding

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Holly L. AARON

Not confocal question: Laser Shielding

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Hi, All - I apologize for the non-confocal nature of this question, but I
cannot think of a better resource than this community. I am looking for
some sort of "tinting" or similar to minimize the laser exposure through a
clear plastic incubation chamber on a TIRF-based rig. Our laser safety
officer suggested tinting would be okay, but I have no idea where to look
for that in custom sizes/amounts. Would window tinting for vehicles work?
Has anyone done this? Would love to hear your experience.

Thanks as always!
-Holly
___________________________
Holly L. Aaron
Molecular Imaging Center
Cancer Research Laboratory
University of California Berkeley
251 LSA #2751
Berkeley, CA 94720-2751
510.642.2901
510.642.5741 fax
[hidden email]
http://imaging.berkeley.edu

*
*

Guy Cox-2

Re: Not confocal question: Laser Shielding

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Holly,

I'm assuming you want to stop the exposure to the operator on an inverted microscope. Is that correct? Most inverted fluorescence microscopes already come with a plastic safety screen for that purpose - isn't that enough? If not I'd suggest getting some theatrical plastic filter. This comes in big sheets, is very cheap, and has guaranteed absorption spectra. A yellow-orange tint should do the trick, but check the spectra to see that it will block your laser lines. You can easily cut bits to fit, and replace them as often as necessary. I can't see that window tint would do much since it's mainly designed to block IR.

Guy

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Holly L. AARON
Sent: Wednesday, 20 March 2013 3:30 AM
To: [hidden email]
Subject: Not confocal question: Laser Shielding

*****
To join, leave or search the confocal microscopy listserv, go to:
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*****

Hi, All - I apologize for the non-confocal nature of this question, but I cannot think of a better resource than this community. I am looking for some sort of "tinting" or similar to minimize the laser exposure through a clear plastic incubation chamber on a TIRF-based rig. Our laser safety officer suggested tinting would be okay, but I have no idea where to look for that in custom sizes/amounts. Would window tinting for vehicles work?
Has anyone done this? Would love to hear your experience.

Thanks as always!
-Holly
___________________________
Holly L. Aaron
Molecular Imaging Center
Cancer Research Laboratory
University of California Berkeley
251 LSA #2751
Berkeley, CA 94720-2751
510.642.2901
510.642.5741 fax
[hidden email]
http://imaging.berkeley.edu

*
*

Gregg Jarvis

Re: Not confocal question: Laser Shielding

In reply to this post by Holly L. AARON

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What WL is the Laser?
You should probably be wearing Laser Safety Goggles.
If you are just trying to minimize scatter so you don't look at it then
window tint might work.
Do they make a tinted incubator that you can buy that is for your scope?
The local Autozone will have different shades of darkness to choose from,
$14

On Tue, Mar 19, 2013 at 12:30 PM, Holly L. AARON <[hidden email]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi, All - I apologize for the non-confocal nature of this question, but I
> cannot think of a better resource than this community. I am looking for
> some sort of "tinting" or similar to minimize the laser exposure through a
> clear plastic incubation chamber on a TIRF-based rig. Our laser safety
> officer suggested tinting would be okay, but I have no idea where to look
> for that in custom sizes/amounts. Would window tinting for vehicles work?
> Has anyone done this? Would love to hear your experience.
>
> Thanks as always!
> -Holly
> ___________________________
> Holly L. Aaron
> Molecular Imaging Center
> Cancer Research Laboratory
> University of California Berkeley
> 251 LSA #2751
> Berkeley, CA 94720-2751
> 510.642.2901
> 510.642.5741 fax
> [hidden email]
> http://imaging.berkeley.edu
>
> *
> *
>

--
Gregg Jarvis
Senior Spectroscopist
Advanced Products Group
Omega Optical Inc.

Delta Campus, 21 Omega Drive
Brattleboro, VT 05301, USA
Tel: +1 (802) 251-7316
[hidden email]

--
"The information in this electronic mail ("e-mail") message may contain
information that is confidential and/or privileged, or may otherwise be
protected by work product or other legal rules. It is solely for the use of
the individual(s) or the entity(ies) originally intended. Access to this
electronic mail message by anyone else is unauthorized. If you are not the
intended recipient, be advised that any unauthorized review, disclosure,
copying, distribution or use of this information, or any action taken or
omitted to be taken in reliance on it, is prohibited and may be unlawful.
Please notify the sender immediately if you have received this electronic
message by mistake, and destroy all copies of the original message."

"The sender believes that this e-mail and any attachments were free of any
virus, worm, Trojan horse, malicious code and/or other contaminants when
sent. E-mail transmissions cannot be guaranteed to be secure or error-free,
so this message and its attachments could have been infected, corrupted or
made incomplete during transmission. By reading the message and opening any
attachments, the recipient accepts full responsibility for any viruses or
other defects that may arise, and for taking remedial action relating to
such viruses and other defects. Neither Omega Optical Inc.<http://www.omegafilters.com>nor any of its affiliated entities is liable for any loss or damage arising
in any way from, or for errors or omissions in the contents of, this
message or its attachments."

Omega Optical Inc. <http://www.omegafilters.com> may monitor all incoming
and outgoing email communications, including the content of emails and
attachments, for purposes of security, legal compliance, training, quality
assurance and other purposes permitted by law.

Gregg Jarvis

Re: Not confocal question: Laser Shielding

In reply to this post by Guy Cox-2

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*****

Great answer by Guy Cox,
Laser blocking theatrical Gel gets sold in sheets, just be sure to get a WL
that blocks your laser

On Tue, Mar 19, 2013 at 1:45 PM, Guy Cox <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Holly,
>
> I'm assuming you want to stop the exposure to the operator on an
> inverted microscope. Is that correct? Most inverted fluorescence
> microscopes already come with a plastic safety screen for that purpose -
> isn't that enough? If not I'd suggest getting some theatrical plastic
> filter. This comes in big sheets, is very cheap, and has guaranteed
> absorption spectra. A yellow-orange tint should do the trick, but check
> the spectra to see that it will block your laser lines. You can easily cut
> bits to fit, and replace them as often as necessary. I can't see that
> window tint would do much since it's mainly designed to block IR.
>
> Guy
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Holly L. AARON
> Sent: Wednesday, 20 March 2013 3:30 AM
> To: [hidden email]
> Subject: Not confocal question: Laser Shielding
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi, All - I apologize for the non-confocal nature of this question, but I
> cannot think of a better resource than this community. I am looking for
> some sort of "tinting" or similar to minimize the laser exposure through a
> clear plastic incubation chamber on a TIRF-based rig. Our laser safety
> officer suggested tinting would be okay, but I have no idea where to look
> for that in custom sizes/amounts. Would window tinting for vehicles work?
> Has anyone done this? Would love to hear your experience.
>
> Thanks as always!
> -Holly
> ___________________________
> Holly L. Aaron
> Molecular Imaging Center
> Cancer Research Laboratory
> University of California Berkeley
> 251 LSA #2751
> Berkeley, CA 94720-2751
> 510.642.2901
> 510.642.5741 fax
> [hidden email]
> http://imaging.berkeley.edu
>
> *
> *
>

Holly L. AARON

Re: Not confocal question: Laser Shielding

In reply to this post by Gregg Jarvis

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Unfortunately a tinted incubator was not ordered as part of this rig and it
is a rather complicated enclosure (lots of ins & outs) and took 3+ fittings
to get it to fit right, so while it would be relatively easy to replicate
now, I would rather not pay the additional expense (and have a useless
clear enclosure)... but that might be the best option.

The primary wavelengths that needs to be blocked are 561nm and 450nm (PALM
scope), but there are also several other wavelength lasers (458, 488, 514,
633nm), although the power is much less on those.

I will check out this theatrical filter - thanks for the idea, Guy!

(and yes, we have laser safety goggles for alignments)

Thanks,

-Holly

(transitioning to bMail... thank you for your patience)

___________________________
Holly L. Aaron
Molecular Imaging Center
Cancer Research Laboratory
University of California Berkeley
251 LSA #2751
Berkeley, CA 94720-2751
510.642.2901
510.642.5741 fax
[hidden email]
http://imaging.berkeley.edu

*
*

On Tue, Mar 19, 2013 at 11:02 AM, Gregg Jarvis <[hidden email]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> What WL is the Laser?
> You should probably be wearing Laser Safety Goggles.
> If you are just trying to minimize scatter so you don't look at it then
> window tint might work.
> Do they make a tinted incubator that you can buy that is for your scope?
> The local Autozone will have different shades of darkness to choose from,
> $14
>
> On Tue, Mar 19, 2013 at 12:30 PM, Holly L. AARON <[hidden email]
> >wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Hi, All - I apologize for the non-confocal nature of this question, but I
> > cannot think of a better resource than this community. I am looking for
> > some sort of "tinting" or similar to minimize the laser exposure through
> a
> > clear plastic incubation chamber on a TIRF-based rig. Our laser safety
> > officer suggested tinting would be okay, but I have no idea where to look
> > for that in custom sizes/amounts. Would window tinting for vehicles work?
> > Has anyone done this? Would love to hear your experience.
> >
> > Thanks as always!
> > -Holly
> > ___________________________
> > Holly L. Aaron
> > Molecular Imaging Center
> > Cancer Research Laboratory
> > University of California Berkeley
> > 251 LSA #2751
> > Berkeley, CA 94720-2751
> > 510.642.2901
> > 510.642.5741 fax
> > [hidden email]
> > http://imaging.berkeley.edu
> >
> > *
> > *
> >
>
>
>
> --
> Gregg Jarvis
> Senior Spectroscopist
> Advanced Products Group
> Omega Optical Inc.
>
> Delta Campus, 21 Omega Drive
> Brattleboro, VT 05301, USA
> Tel: +1 (802) 251-7316
> [hidden email]
>
> --
> "The information in this electronic mail ("e-mail") message may contain
> information that is confidential and/or privileged, or may otherwise be
> protected by work product or other legal rules. It is solely for the use of
> the individual(s) or the entity(ies) originally intended. Access to this
> electronic mail message by anyone else is unauthorized. If you are not the
> intended recipient, be advised that any unauthorized review, disclosure,
> copying, distribution or use of this information, or any action taken or
> omitted to be taken in reliance on it, is prohibited and may be unlawful.
> Please notify the sender immediately if you have received this electronic
> message by mistake, and destroy all copies of the original message."
>
> "The sender believes that this e-mail and any attachments were free of any
> virus, worm, Trojan horse, malicious code and/or other contaminants when
> sent. E-mail transmissions cannot be guaranteed to be secure or error-free,
> so this message and its attachments could have been infected, corrupted or
> made incomplete during transmission. By reading the message and opening any
> attachments, the recipient accepts full responsibility for any viruses or
> other defects that may arise, and for taking remedial action relating to
> such viruses and other defects. Neither Omega Optical Inc.<
> http://www.omegafilters.com>nor any of its affiliated entities is liable
> for any loss or damage arising
> in any way from, or for errors or omissions in the contents of, this
> message or its attachments."
>
> Omega Optical Inc. <http://www.omegafilters.com> may monitor all incoming
> and outgoing email communications, including the content of emails and
> attachments, for purposes of security, legal compliance, training, quality
> assurance and other purposes permitted by law.
>

mcammer

Re: Not confocal question: Laser Shielding

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

We have black plastic sheeting cut into pieces and velcroed on the outside of the box that we use for multiphoton. You could completely enclose the box but use black tape instead of Velcro.

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Holly L. AARON
Sent: Tuesday, March 19, 2013 3:05 PM
To: [hidden email]
Subject: Re: Not confocal question: Laser Shielding

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Unfortunately a tinted incubator was not ordered as part of this rig and it is a rather complicated enclosure (lots of ins & outs) and took 3+ fittings to get it to fit right, so while it would be relatively easy to replicate now, I would rather not pay the additional expense (and have a useless clear enclosure)... but that might be the best option.

The primary wavelengths that needs to be blocked are 561nm and 450nm (PALM scope), but there are also several other wavelength lasers (458, 488, 514, 633nm), although the power is much less on those.

I will check out this theatrical filter - thanks for the idea, Guy!

(and yes, we have laser safety goggles for alignments)

Thanks,

-Holly

(transitioning to bMail... thank you for your patience)

___________________________
Holly L. Aaron
Molecular Imaging Center
Cancer Research Laboratory
University of California Berkeley
251 LSA #2751
Berkeley, CA 94720-2751
510.642.2901
510.642.5741 fax
[hidden email]
http://imaging.berkeley.edu

*
*

On Tue, Mar 19, 2013 at 11:02 AM, Gregg Jarvis <[hidden email]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> What WL is the Laser?
> You should probably be wearing Laser Safety Goggles.
> If you are just trying to minimize scatter so you don't look at it
> then window tint might work.
> Do they make a tinted incubator that you can buy that is for your scope?
> The local Autozone will have different shades of darkness to choose
> from,
> $14
>
> On Tue, Mar 19, 2013 at 12:30 PM, Holly L. AARON <[hidden email]
> >wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Hi, All - I apologize for the non-confocal nature of this question,
> > but I cannot think of a better resource than this community. I am
> > looking for some sort of "tinting" or similar to minimize the laser
> > exposure through
> a
> > clear plastic incubation chamber on a TIRF-based rig. Our laser
> > safety officer suggested tinting would be okay, but I have no idea
> > where to look for that in custom sizes/amounts. Would window tinting for vehicles work?
> > Has anyone done this? Would love to hear your experience.
> >
> > Thanks as always!
> > -Holly
> > ___________________________
> > Holly L. Aaron
> > Molecular Imaging Center
> > Cancer Research Laboratory
> > University of California Berkeley
> > 251 LSA #2751
> > Berkeley, CA 94720-2751
> > 510.642.2901
> > 510.642.5741 fax
> > [hidden email]
> > http://imaging.berkeley.edu
> >
> > *
> > *
> >
>
>
>
> --
> Gregg Jarvis
> Senior Spectroscopist
> Advanced Products Group
> Omega Optical Inc.
>
> Delta Campus, 21 Omega Drive
> Brattleboro, VT 05301, USA
> Tel: +1 (802) 251-7316
> [hidden email]
>
> --
> "The information in this electronic mail ("e-mail") message may
> contain information that is confidential and/or privileged, or may
> otherwise be protected by work product or other legal rules. It is
> solely for the use of the individual(s) or the entity(ies) originally
> intended. Access to this electronic mail message by anyone else is
> unauthorized. If you are not the intended recipient, be advised that
> any unauthorized review, disclosure, copying, distribution or use of
> this information, or any action taken or omitted to be taken in reliance on it, is prohibited and may be unlawful.
> Please notify the sender immediately if you have received this
> electronic message by mistake, and destroy all copies of the original message."
>
> "The sender believes that this e-mail and any attachments were free of
> any virus, worm, Trojan horse, malicious code and/or other
> contaminants when sent. E-mail transmissions cannot be guaranteed to
> be secure or error-free, so this message and its attachments could
> have been infected, corrupted or made incomplete during transmission.
> By reading the message and opening any attachments, the recipient
> accepts full responsibility for any viruses or other defects that may
> arise, and for taking remedial action relating to such viruses and
> other defects. Neither Omega Optical Inc.<
> http://www.omegafilters.com>nor any of its affiliated entities is
> liable for any loss or damage arising in any way from, or for errors
> or omissions in the contents of, this message or its attachments."
>
> Omega Optical Inc. <http://www.omegafilters.com> may monitor all
> incoming and outgoing email communications, including the content of
> emails and attachments, for purposes of security, legal compliance,
> training, quality assurance and other purposes permitted by law.
>

Benjamin Hibbs

Re: Not confocal question: Laser Shielding

In reply to this post by Holly L. AARON

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi Holly,

I'm not sure how your stage is set up, but we have a blockout plate that covers the stage and also acts as a laser interlock on our TIRF (Nikon N-STORM) system. However, we don't have an incubator. Would it be possible for you to enclose the sample within the incubator? It might be simpler than covering the incubator and would still have the advantage of blocking any stray room light (even light from the computer monitors gets picked up by our EMCCD if it isn't blocked).

And thanks Guy, for the theatrical filter tip. Very handy.

Cheers,

Ben

Ben Hibbs
Platform Support Officer—Advanced Fluorescence Imaging
The Melbourne Materials Institute (MMI)
University of Melbourne, Victoria 3010, Australia
Email: [hidden email]<mailto:[hidden email]>

On 20/03/2013, at 6:05 AM, Holly L. AARON <[hidden email]<mailto:[hidden email]>> wrote:

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Unfortunately a tinted incubator was not ordered as part of this rig and it
is a rather complicated enclosure (lots of ins & outs) and took 3+ fittings
to get it to fit right, so while it would be relatively easy to replicate
now, I would rather not pay the additional expense (and have a useless
clear enclosure)... but that might be the best option.

The primary wavelengths that needs to be blocked are 561nm and 450nm (PALM
scope), but there are also several other wavelength lasers (458, 488, 514,
633nm), although the power is much less on those.

I will check out this theatrical filter - thanks for the idea, Guy!

(and yes, we have laser safety goggles for alignments)

Thanks,

-Holly

(transitioning to bMail... thank you for your patience)

___________________________
Holly L. Aaron
Molecular Imaging Center
Cancer Research Laboratory
University of California Berkeley
251 LSA #2751
Berkeley, CA 94720-2751
510.642.2901
510.642.5741 fax
[hidden email]
http://imaging.berkeley.edu

*
*

On Tue, Mar 19, 2013 at 11:02 AM, Gregg Jarvis <[hidden email]>wrote:

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

What WL is the Laser?
You should probably be wearing Laser Safety Goggles.
If you are just trying to minimize scatter so you don't look at it then
window tint might work.
Do they make a tinted incubator that you can buy that is for your scope?
The local Autozone will have different shades of darkness to choose from,
$14

On Tue, Mar 19, 2013 at 12:30 PM, Holly L. AARON <[hidden email]
wrote:

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi, All - I apologize for the non-confocal nature of this question, but I
cannot think of a better resource than this community. I am looking for
some sort of "tinting" or similar to minimize the laser exposure through
a
clear plastic incubation chamber on a TIRF-based rig. Our laser safety
officer suggested tinting would be okay, but I have no idea where to look
for that in custom sizes/amounts. Would window tinting for vehicles work?
Has anyone done this? Would love to hear your experience.

Thanks as always!
-Holly
___________________________
Holly L. Aaron
Molecular Imaging Center
Cancer Research Laboratory
University of California Berkeley
251 LSA #2751
Berkeley, CA 94720-2751
510.642.2901
510.642.5741 fax
[hidden email]
http://imaging.berkeley.edu

*
*

--
Gregg Jarvis
Senior Spectroscopist
Advanced Products Group
Omega Optical Inc.

Delta Campus, 21 Omega Drive
Brattleboro, VT 05301, USA
Tel: +1 (802) 251-7316
[hidden email]

--
"The information in this electronic mail ("e-mail") message may contain
information that is confidential and/or privileged, or may otherwise be
protected by work product or other legal rules. It is solely for the use of
the individual(s) or the entity(ies) originally intended. Access to this
electronic mail message by anyone else is unauthorized. If you are not the
intended recipient, be advised that any unauthorized review, disclosure,
copying, distribution or use of this information, or any action taken or
omitted to be taken in reliance on it, is prohibited and may be unlawful.
Please notify the sender immediately if you have received this electronic
message by mistake, and destroy all copies of the original message."

"The sender believes that this e-mail and any attachments were free of any
virus, worm, Trojan horse, malicious code and/or other contaminants when
sent. E-mail transmissions cannot be guaranteed to be secure or error-free,
so this message and its attachments could have been infected, corrupted or
made incomplete during transmission. By reading the message and opening any
attachments, the recipient accepts full responsibility for any viruses or
other defects that may arise, and for taking remedial action relating to
such viruses and other defects. Neither Omega Optical Inc.<
http://www.omegafilters.com>nor any of its affiliated entities is liable
for any loss or damage arising
in any way from, or for errors or omissions in the contents of, this
message or its attachments."

Omega Optical Inc. <http://www.omegafilters.com> may monitor all incoming
and outgoing email communications, including the content of emails and
attachments, for purposes of security, legal compliance, training, quality
assurance and other purposes permitted by law.