Nuance Efficiency Curve
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Does anyone know what the light transmission efficiency of the Nuance multispectral imaging system from Perkin Elmer is? I've been trying to find a % transmission curve for the liquid crystal filter but have not been able to. Sincerely, Claire |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Liquid crystal filters usually only work with polarized light, so the first part of the filter system is usually a linear polarizer. This will immediately take out half your light, assuming unpolarized light from your sample. The LC also has a reduced transmission on top of that so overall the system efficiencies will be mediocre at best. Craig On Jun 21, 2016 9:24 AM, "Claire Brown, Dr." <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Does anyone know what the light transmission efficiency of the Nuance > multispectral imaging system from Perkin Elmer is? > I've been trying to find a % transmission curve for the liquid crystal > filter but have not been able to. > > Sincerely, > > Claire > |
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In reply to this post by Claire Brown
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Claire, Figure 1, top panel, is the relevant curve for the Nuance and Vectra LCTF (note this is for properly polarized light, divide Y axis byt 2 for non-polarized light). http://www.spectralcameras.com/files/VariSpec_Brochure.pdf Craig noted in his reply: "Liquid crystal filters usually only work with polarized light, so the first part of the filter system is usually a linear polarizer. This will immediately take out half your light, assuming unpolarized light from your sample. The LC also has a reduced transmission on top of that so overall the system efficiencies will be mediocre at best" Richard Levenson, Joe Beechem, and I wrote a review (open access) on spectral imaging that discusses various options (a fast alternative, AOTF, is also polarization dependent): http://www.hindawi.com/journals/acp/2012/904828/abs/ and finishes with my favorite "SKY400" chromosome paints image (in answer to another thread: Applied Spectral Imaging and several other companies sell each and all human chromosome paint probe sets). As for section 14 "going for 100-plex" ... hopefully Garry Nolan will get his "MIBI-TOF" paper out (probably only 40plex; near single ion sensitivity, which is fine since each antibody has ~180 mass tags). enjoy, George p.s. LCTFing non-polarized light, PerkinElmer could use a polarizing beamsplitter to split the V and H pol, put those through separate LCTFs (of proper orientations), then recombine onto one camera ("Fluorospec"), or send to two cameras. The latter would be a lot of fun for "spectral fluorescence anisotropy". On 6/20/2016 1:33 PM, Claire Brown, Dr. wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Does anyone know what the light transmission efficiency of the Nuance multispectral imaging system from Perkin Elmer is? > I've been trying to find a % transmission curve for the liquid crystal filter but have not been able to. > > Sincerely, > > Claire > -- George McNamara, Ph.D. Houston, TX 77054 [hidden email] 713-239-0365 home 305-764-2081 cell https://www.linkedin.com/in/georgemcnamara https://works.bepress.com/gmcnamara/75/ Tattletales and T-Bow |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hello, The Varispec LCTF is only half the part of the nuance/Vectra camera. You still need to factor in the QE of the CCD chip. I vaguely recall seeing a PPT presentation that the Nuance uses a Sony ICX285 (or similar) chip. So max QE will be around ~70% at 500 nm. HTH. Sripad On Tue, Jun 21, 2016 at 11:13 AM, George McNamara <[hidden email] > wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Claire, > > Figure 1, top panel, is the relevant curve for the Nuance and Vectra LCTF > (note this is for properly polarized light, divide Y axis byt 2 for > non-polarized light). > > http://www.spectralcameras.com/files/VariSpec_Brochure.pdf > > Craig noted in his reply: "Liquid crystal filters usually only work with > polarized light, so the first part of the filter system is usually a linear > polarizer. This will immediately take out half your light, assuming > unpolarized light from your sample. The LC also has a reduced transmission > on top of that so overall the system efficiencies will be mediocre at best" > > Richard Levenson, Joe Beechem, and I wrote a review (open access) on > spectral imaging that discusses various options (a fast alternative, AOTF, > is also polarization dependent): > > http://www.hindawi.com/journals/acp/2012/904828/abs/ > > and finishes with my favorite "SKY400" chromosome paints image (in answer > to another thread: Applied Spectral Imaging and several other companies > sell each and all human chromosome paint probe sets). As for section 14 > "going for 100-plex" ... hopefully Garry Nolan will get his "MIBI-TOF" > paper out (probably only 40plex; near single ion sensitivity, which is fine > since each antibody has ~180 mass tags). > > enjoy, > > George > p.s. LCTFing non-polarized light, PerkinElmer could use a polarizing > beamsplitter to split the V and H pol, put those through separate LCTFs (of > proper orientations), then recombine onto one camera ("Fluorospec"), or > send to two cameras. The latter would be a lot of fun for "spectral > fluorescence anisotropy". > > > > On 6/20/2016 1:33 PM, Claire Brown, Dr. wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> Post images on http://www.imgur.com and include the link in your posting. >> ***** >> >> Does anyone know what the light transmission efficiency of the Nuance >> multispectral imaging system from Perkin Elmer is? >> I've been trying to find a % transmission curve for the liquid crystal >> filter but have not been able to. >> >> Sincerely, >> >> Claire >> >> > > -- > > > > George McNamara, Ph.D. > Houston, TX 77054 > [hidden email] > 713-239-0365 home > 305-764-2081 cell > https://www.linkedin.com/in/georgemcnamara > https://works.bepress.com/gmcnamara/75/ Tattletales and T-Bow > |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Claire, It's been a long time....I hope all is well;-) Another thing besides the QE with the Nuance camera is that it is only a 10 bit camera which severely limits the dynamic range. I have a lab at Stanford that I installed a Flash 4.0 V2 in with a dual adapter and both the cameras are running in Metamorph. Besides the FOV and dynamic range they work together well. I have a great contact in PE that was around when CRi developed the Nuance and would be happy to share if you'd be interested? Loralei Dewe Chrysalis Innovations On Jun 21, 2016 1:34 PM, "S Ram" <[hidden email]> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hello, The Varispec LCTF is only half the part of the nuance/Vectra camera. You still need to factor in the QE of the CCD chip. I vaguely recall seeing a PPT presentation that the Nuance uses a Sony ICX285 (or similar) chip. So max QE will be around ~70% at 500 nm. HTH. Sripad On Tue, Jun 21, 2016 at 11:13 AM, George McNamara <[hidden email] > wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Claire, > > Figure 1, top panel, is the relevant curve for the Nuance and Vectra LCTF > (note this is for properly polarized light, divide Y axis byt 2 for > non-polarized light). > > http://www.spectralcameras.com/files/VariSpec_Brochure.pdf > > Craig noted in his reply: "Liquid crystal filters usually only work with > polarized light, so the first part of the filter system is usually a > polarizer. This will immediately take out half your light, assuming > unpolarized light from your sample. The LC also has a reduced transmission > on top of that so overall the system efficiencies will be mediocre at best" > > Richard Levenson, Joe Beechem, and I wrote a review (open access) on > spectral imaging that discusses various options (a fast alternative, AOTF, > is also polarization dependent): > > http://www.hindawi.com/journals/acp/2012/904828/abs/ > > and finishes with my favorite "SKY400" chromosome paints image (in answer > to another thread: Applied Spectral Imaging and several other companies > sell each and all human chromosome paint probe sets). As for section 14 > "going for 100-plex" ... hopefully Garry Nolan will get his "MIBI-TOF" > paper out (probably only 40plex; near single ion sensitivity, which is > since each antibody has ~180 mass tags). > > enjoy, > > George > p.s. LCTFing non-polarized light, PerkinElmer could use a polarizing > beamsplitter to split the V and H pol, put those through separate LCTFs (of > proper orientations), then recombine onto one camera ("Fluorospec"), or > send to two cameras. The latter would be a lot of fun for "spectral > fluorescence anisotropy". > > > > On 6/20/2016 1:33 PM, Claire Brown, Dr. wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> Post images on http://www.imgur.com and include the link in your posting. >> ***** >> >> Does anyone know what the light transmission efficiency of the Nuance >> multispectral imaging system from Perkin Elmer is? >> I've been trying to find a % transmission curve for the liquid crystal >> filter but have not been able to. >> >> Sincerely, >> >> Claire >> >> > > -- > > > > George McNamara, Ph.D. > Houston, TX 77054 > [hidden email] > 713-239-0365 home > 305-764-2081 cell > https://www.linkedin.com/in/georgemcnamara > https://works.bepress.com/gmcnamara/75/ Tattletales and T-Bow > |
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