Nunc Lab-Tek problem
Locked
|
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi. For many years we used Nunc Lab-Tek chambered coverglasses. They were great. However, they recently began shipping a different design with the same product number. The chambers no longer fit in our microscope stages inserts. Also, the volume changed. see http://www.flickr.com/photos/mcammer/sets/72157632984171809/ While we can adjust to the volume change, the plastic tab at the end of the chamber is a big problem. I thought of having people cut the tab off before doing experiments, but this would produce a lot of plastic dust (and we'd have to buy a suitable saw or dremel). Does anybody know of an alternative manufacturer or if the old style chambers are still available from somewhere? Thanks! Michael ________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270 |
|
|
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Michael, The difference is between the Labtek and the Labtek II version of the chambered coverglass. The first one has the no. 1 and the second has the no 1.5 coverglass bottom. As far as I know both models are still available (at least over here..). Whenever we us the II version, we simply cut off the tab with an ordinary wire-shear. Best regards Els -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Cammer, Michael Sent: woensdag 13 maart 2013 16:17 To: [hidden email] Subject: [CONFOCALMICROSCOPY] Nunc Lab-Tek problem ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi. For many years we used Nunc Lab-Tek chambered coverglasses. They were great. However, they recently began shipping a different design with the same product number. The chambers no longer fit in our microscope stages inserts. Also, the volume changed. see http://www.flickr.com/photos/mcammer/sets/72157632984171809/ While we can adjust to the volume change, the plastic tab at the end of the chamber is a big problem. I thought of having people cut the tab off before doing experiments, but this would produce a lot of plastic dust (and we'd have to buy a suitable saw or dremel). Does anybody know of an alternative manufacturer or if the old style chambers are still available from somewhere? Thanks! Michael ________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270 |
|
|
In reply to this post by mcammer
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Michael, Look at ibidi they have quite a few designs and may be some can fit your requirements. No commercial interest. Deepak On Wed, Mar 13, 2013 at 8:47 PM, Cammer, Michael <[hidden email] > wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi. > > For many years we used Nunc Lab-Tek chambered coverglasses. They were > great. > > However, they recently began shipping a different design with the same > product number. The chambers no longer fit in our microscope stages > inserts. Also, the volume changed. > see http://www.flickr.com/photos/mcammer/sets/72157632984171809/ > > While we can adjust to the volume change, the plastic tab at the end of > the chamber is a big problem. > > I thought of having people cut the tab off before doing experiments, but > this would produce a lot of plastic dust (and we'd have to buy a suitable > saw or dremel). > > Does anybody know of an alternative manufacturer or if the old style > chambers are still available from somewhere? > > Thanks! > > Michael > > > > > ________________________________________________________ > Michael Cammer, Assistant Research Scientist > Skirball Institute of Biomolecular Medicine > Lab: (212) 263-3208 Cell: (914) 309-3270 > -- ---------------------------------------------------------------------------------------------------- Deepak K Saini, PhD Assistant Professor Department of Molecular Reproduction, Development and Genetics Indian Institute of Science (IISc) Bangalore, India. [hidden email] [hidden email] Tel: 080-22932574 ------------------------------------------------------------------------------------------------- <[hidden email]> |
 
|
G. Esteban Fernandez |
Re: Nunc Lab-Tek problem
|
|
In reply to this post by els vanstreels
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I use Labtek I (#1.0 glass) and they do not have the tabs. Recently ordered #1 chambers still fit in my ~7 year old LabTek-specific warming stage insert. -Esteban On Wed, Mar 13, 2013 at 8:53 AM, els vanstreels < [hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi Michael, > The difference is between the Labtek and the Labtek II version of the > chambered coverglass. The first one has the no. 1 and the second has the no > 1.5 coverglass bottom. As far as I know both models are still available > (at > least over here..). Whenever we us the II version, we simply cut off the > tab with an ordinary wire-shear. > > Best regards > > Els > > > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] > On > Behalf Of Cammer, Michael > Sent: woensdag 13 maart 2013 16:17 > To: [hidden email] > Subject: [CONFOCALMICROSCOPY] Nunc Lab-Tek problem > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi. > > For many years we used Nunc Lab-Tek chambered coverglasses. They were > great. > > However, they recently began shipping a different design with the same > product number. The chambers no longer fit in our microscope stages > inserts. > Also, the volume changed. > see http://www.flickr.com/photos/mcammer/sets/72157632984171809/ > > While we can adjust to the volume change, the plastic tab at the end of the > chamber is a big problem. > > I thought of having people cut the tab off before doing experiments, but > this would produce a lot of plastic dust (and we'd have to buy a suitable > saw or dremel). > > Does anybody know of an alternative manufacturer or if the old style > chambers are still available from somewhere? > > Thanks! > > Michael > > > > > ________________________________________________________ > Michael Cammer, Assistant Research Scientist Skirball Institute of > Biomolecular Medicine > Lab: (212) 263-3208 Cell: (914) 309-3270 > |
|
|
In reply to this post by Deepak Saini
Hi Michael,
Be aware that Ibidi slides bottom are not made of glass and will interfere with a lot of reflection during imaging. Hope this will help, Myriam Myriam Gastard, PhD From my iPad. On Mar 13, 2013, at 12:08 PM, "Deepak Saini" <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi Michael, > > Look at ibidi they have quite a few designs and may be some can fit your > requirements. > > No commercial interest. > > Deepak > > > On Wed, Mar 13, 2013 at 8:47 PM, Cammer, Michael <[hidden email] >> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Hi. >> >> For many years we used Nunc Lab-Tek chambered coverglasses. They were >> great. >> >> However, they recently began shipping a different design with the same >> product number. The chambers no longer fit in our microscope stages >> inserts. Also, the volume changed. >> see http://www.flickr.com/photos/mcammer/sets/72157632984171809/ >> >> While we can adjust to the volume change, the plastic tab at the end of >> the chamber is a big problem. >> >> I thought of having people cut the tab off before doing experiments, but >> this would produce a lot of plastic dust (and we'd have to buy a suitable >> saw or dremel). >> >> Does anybody know of an alternative manufacturer or if the old style >> chambers are still available from somewhere? >> >> Thanks! >> >> Michael >> >> >> >> >> ________________________________________________________ >> Michael Cammer, Assistant Research Scientist >> Skirball Institute of Biomolecular Medicine >> Lab: (212) 263-3208 Cell: (914) 309-3270 >> > > > > -- > ---------------------------------------------------------------------------------------------------- > Deepak K Saini, PhD > Assistant Professor > Department of Molecular Reproduction, Development and Genetics > Indian Institute of Science (IISc) > Bangalore, India. > [hidden email] > [hidden email] > Tel: 080-22932574 > ------------------------------------------------------------------------------------------------- > <[hidden email]> |
|
|
In reply to this post by els vanstreels
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Thank you for the many replies. Our sales rep just stopped in and the email below explains the problem. People in the lab were using the #1 coverslip bottom wells and because of an issue on the TIRF microscope and my formal microscopy training, I insisted we switch to 1.5 coverslips. We did not realize that the entire design was different. According to the sales rep, both the old design as #1 and the new design as #1.5 are available. So I'm going to recommend that for normal confocal etc. people use the #1 chambers even though the optics aren't perfect. And we will try snipping off or otherwise removing the tabs on the newer models. Thanks again! Regards, Michael -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of els vanstreels Sent: Wednesday, March 13, 2013 11:54 AM To: [hidden email] Subject: Re: Nunc Lab-Tek problem ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Michael, The difference is between the Labtek and the Labtek II version of the chambered coverglass. The first one has the no. 1 and the second has the no 1.5 coverglass bottom. As far as I know both models are still available (at least over here..). Whenever we us the II version, we simply cut off the tab with an ordinary wire-shear. Best regards Els -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Cammer, Michael Sent: woensdag 13 maart 2013 16:17 To: [hidden email] Subject: [CONFOCALMICROSCOPY] Nunc Lab-Tek problem ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi. For many years we used Nunc Lab-Tek chambered coverglasses. They were great. However, they recently began shipping a different design with the same product number. The chambers no longer fit in our microscope stages inserts. Also, the volume changed. see http://www.flickr.com/photos/mcammer/sets/72157632984171809/ While we can adjust to the volume change, the plastic tab at the end of the chamber is a big problem. I thought of having people cut the tab off before doing experiments, but this would produce a lot of plastic dust (and we'd have to buy a suitable saw or dremel). Does anybody know of an alternative manufacturer or if the old style chambers are still available from somewhere? Thanks! Michael ________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270 |
|
|
In reply to this post by Myriam Gastard
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Ibidi makes many versions of chambers. Some do have glass bottoms. We routinely use two designs in our lab with #1.5 coverslips on the bottom, but we want the larger open chambers for other experiments. Regards, Michael -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Myriam Gastard Sent: Wednesday, March 13, 2013 2:10 PM To: [hidden email] Subject: Re: Nunc Lab-Tek problem Hi Michael, Be aware that Ibidi slides bottom are not made of glass and will interfere with a lot of reflection during imaging. Hope this will help, Myriam Myriam Gastard, PhD From my iPad. On Mar 13, 2013, at 12:08 PM, "Deepak Saini" <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi Michael, > > Look at ibidi they have quite a few designs and may be some can fit > your requirements. > > No commercial interest. > > Deepak > > > On Wed, Mar 13, 2013 at 8:47 PM, Cammer, Michael > <[hidden email] >> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Hi. >> >> For many years we used Nunc Lab-Tek chambered coverglasses. They were >> great. >> >> However, they recently began shipping a different design with the >> same product number. The chambers no longer fit in our microscope >> stages inserts. Also, the volume changed. >> see http://www.flickr.com/photos/mcammer/sets/72157632984171809/ >> >> While we can adjust to the volume change, the plastic tab at the end >> of the chamber is a big problem. >> >> I thought of having people cut the tab off before doing experiments, >> but this would produce a lot of plastic dust (and we'd have to buy a >> suitable saw or dremel). >> >> Does anybody know of an alternative manufacturer or if the old style >> chambers are still available from somewhere? >> >> Thanks! >> >> Michael >> >> >> >> >> ________________________________________________________ >> Michael Cammer, Assistant Research Scientist Skirball Institute of >> Biomolecular Medicine >> Lab: (212) 263-3208 Cell: (914) 309-3270 >> > > > > -- > ---------------------------------------------------------------------- > ------------------------------ > Deepak K Saini, PhD > Assistant Professor > Department of Molecular Reproduction, Development and Genetics Indian > Institute of Science (IISc) Bangalore, India. > [hidden email] > [hidden email] > Tel: 080-22932574 > ---------------------------------------------------------------------- > --------------------------- > <[hidden email]> |
|
|
Hi Michael,
I am glad you didn't get any interferences while using your slides. I have tested extensively some of their multi-chamber slides I got huge reflection not only from the plastic bottom but also from the well walls. It is to be noted that it was tested in confocal and using STED and the high power CW laser could have contributed to that obviously. They do say that while their bottom is a #1.5, it is not a standard glass. I had some discussions with Ibidi and they were then quite surprised by the results. Still, I have not yet tried a non-standard bottom and would be glad to try them. Nevertheless, if their bottom cover has not triggered problems in your experiments, then maybe you can try 1 or 2 wells slides which have a big cell adhesion surface (no commercial interest!) ibidi Standard Bottom The ibidi Standard Bottom, which is our standard surface, is a hydrophilic, foil-like coverslip that forms the bottom of most ibidi μ-Slides, μ-Dishes, and μ-Plates. The Standard Bottom meets all optical requirements for various imaging techniques, such as phase contrast,confocal fluorescence, or two-photon microscopy. Best, Myriam Myriam Gastard, PhD From my iPad. On Mar 13, 2013, at 3:23 PM, "Cammer, Michael" <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Ibidi makes many versions of chambers. Some do have glass bottoms. We routinely use two designs in our lab with #1.5 coverslips on the bottom, but we want the larger open chambers for other experiments. > > Regards, > > Michael > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Myriam Gastard > Sent: Wednesday, March 13, 2013 2:10 PM > To: [hidden email] > Subject: Re: Nunc Lab-Tek problem > > Hi Michael, > > Be aware that Ibidi slides bottom are not made of glass and will interfere with a lot of reflection during imaging. > Hope this will help, > > Myriam > > Myriam Gastard, PhD > From my iPad. > > On Mar 13, 2013, at 12:08 PM, "Deepak Saini" <[hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Hi Michael, >> >> Look at ibidi they have quite a few designs and may be some can fit >> your requirements. >> >> No commercial interest. >> >> Deepak >> >> >> On Wed, Mar 13, 2013 at 8:47 PM, Cammer, Michael >> <[hidden email] >>> wrote: >> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> ***** >>> >>> Hi. >>> >>> For many years we used Nunc Lab-Tek chambered coverglasses. They were >>> great. >>> >>> However, they recently began shipping a different design with the >>> same product number. The chambers no longer fit in our microscope >>> stages inserts. Also, the volume changed. >>> see http://www.flickr.com/photos/mcammer/sets/72157632984171809/ >>> >>> While we can adjust to the volume change, the plastic tab at the end >>> of the chamber is a big problem. >>> >>> I thought of having people cut the tab off before doing experiments, >>> but this would produce a lot of plastic dust (and we'd have to buy a >>> suitable saw or dremel). >>> >>> Does anybody know of an alternative manufacturer or if the old style >>> chambers are still available from somewhere? >>> >>> Thanks! >>> >>> Michael >>> >>> >>> >>> >>> ________________________________________________________ >>> Michael Cammer, Assistant Research Scientist Skirball Institute of >>> Biomolecular Medicine >>> Lab: (212) 263-3208 Cell: (914) 309-3270 >>> >> >> >> >> -- >> ---------------------------------------------------------------------- >> ------------------------------ >> Deepak K Saini, PhD >> Assistant Professor >> Department of Molecular Reproduction, Development and Genetics Indian >> Institute of Science (IISc) Bangalore, India. >> [hidden email] >> [hidden email] >> Tel: 080-22932574 >> ---------------------------------------------------------------------- >> --------------------------- >> <[hidden email]> |
|
|
In reply to this post by mcammer
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I use these #1.5 Labtek II slides routinely for intracellular FCS measurements, without removing the tab. The trick is that you place these chambers in the perpendicular direction in your standard holder and not in the usual one. Standard: long side from left to right, if you look over the eyepieces on your stage Labtek II: short side from left to right and long side from the eyepieces to the brightfield arm At least in our standard holders from Zeiss and Leica they fit quiet well in that way, the only problem is that you can not use the whole area of the outer chambers, because (especially with High NA Zeiss objectives) the objective will touch the stage holder. Bye Wolfgang Am 13.03.2013 20:11, schrieb Cammer, Michael: > > Thank you for the many replies. > > Our sales rep just stopped in and the email below explains the problem. > > People in the lab were using the #1 coverslip bottom wells and because > of an issue on the TIRF microscope and my formal microscopy training, > I insisted we switch to 1.5 coverslips. We did not realize that the > entire design was different. > > According to the sales rep, both the old design as #1 and the new > design as #1.5 are available. So I'm going to recommend that for > normal confocal etc. people use the #1 chambers even though the optics > aren't perfect. And we will try snipping off or otherwise removing > the tabs on the newer models. > > Thanks again! > > Regards, > > Michael > > > > > -----Original Message----- > From: Confocal Microscopy List > [mailto:[hidden email]] On Behalf Of els vanstreels > Sent: Wednesday, March 13, 2013 11:54 AM > To: [hidden email] > Subject: Re: Nunc Lab-Tek problem > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=nfocalmicroscopy > ***** > > Hi Michael, > The difference is between the Labtek and the Labtek II version of the > chambered coverglass. The first one has the no. 1 and the second has > the no > 1.5 coverglass bottom. As far as I know both models are still > available (at least over here..). Whenever we us the II version, we > simply cut off the tab with an ordinary wire-shear. > > Best regards > > Els > > > > > -----Original Message----- > From: Confocal Microscopy List > [mailto:[hidden email]] On Behalf Of Cammer, Michael > Sent: woensdag 13 maart 2013 16:17 > To: [hidden email] > Subject: [CONFOCALMICROSCOPY] Nunc Lab-Tek problem > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=nfocalmicroscopy > ***** > > Hi. > > For many years we used Nunc Lab-Tek chambered coverglasses. They were > great. > > However, they recently began shipping a different design with the same > product number. The chambers no longer fit in our microscope stages > inserts. > Also, the volume changed. > see http://www.flickr.com/photos/mcammer/sets/72157632984171809/ > > While we can adjust to the volume change, the plastic tab at the end > of the chamber is a big problem. > > I thought of having people cut the tab off before doing experiments, > but this would produce a lot of plastic dust (and we'd have to buy a > suitable saw or dremel). > > Does anybody know of an alternative manufacturer or if the old style > chambers are still available from somewhere? > > Thanks! > > Michael > > > > > ________________________________________________________ > Michael Cammer, Assistant Research Scientist Skirball Institute of > Biomolecular Medicine > Lab: (212) 263-3208 Cell: (914) 309-3270 > </pre></body> > </html> > </html> -- Dr. Wolfgang Staroske Single Molecule Specialist Light Microscopy Facility Technische Universität Dresden Biotechnology Center Tatzberg 47/49 01307 Dresden, Germany Tel.: +49 (0) 351 463-40316 Fax.: +49 (0) 351 463-40342 E-Mail: [hidden email] Webpage: www.biotec.tu-dresden.de |
|
|
In reply to this post by mcammer
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Nunc Lab-Tek and Lab-Tek II users In that these devices are used primarily for the optical compatibility of the multi-well coverslip surfaces and often used for time-lapse applications. I thought you might be interested to know that Bioptechs has developed a means of providing peripheral heat support without inducing the typical Z axis thermal drift common to heated stages. Information on how it works available at the following link: http://www.bioptechs.com/Products/Stable_Z/Stable_Z.html#labtek For more detailed information, scroll up or use this link: http://www.bioptechs.com/Products/Stable_Z/Stable_Z.html Dan On Mar 13, 2013, at 11:17 AM, Cammer, Michael wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi. For many years we used Nunc Lab-Tek chambered coverglasses. They were great. However, they recently began shipping a different design with the same product number. The chambers no longer fit in our microscope stages inserts. Also, the volume changed. see http://www.flickr.com/photos/mcammer/sets/72157632984171809/ While we can adjust to the volume change, the plastic tab at the end of the chamber is a big problem. I thought of having people cut the tab off before doing experiments, but this would produce a lot of plastic dust (and we'd have to buy a suitable saw or dremel). Does anybody know of an alternative manufacturer or if the old style chambers are still available from somewhere? Thanks! Michael ________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270 Dan Focht Bioptechs, Inc. 3560 Beck Rd. Butler, PA 16002 www.bioptechs.com P: (724)282-7145 F: (724)282-0745 [hidden email] |
|
|
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** After all the angst about the new chambers with tabs at the end, we came up with a very simple cheap solution. We just clip the ends off. It's low tech but works. Thank you for the discussion without which we would not have come upon this simple solution. Anyone interested can see http://www.flickr.com/photos/mcammer/8561005516/ followed by http://www.flickr.com/photos/mcammer/8561005190/ ________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270 |
«
Return to Confocal Microscopy List
|
1 view|%1 views
| Free forum by Nabble | Edit this page |