Objective - maximum laser power
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Steffen Steinert |
Objective - maximum laser power
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Hello, I was wondering whether someone tried to
focus the excitation laser beam to the backfocal plane of the objective (60xoil)
for widefield illumination. If so what was the maximum power used without
affecting the functionality of the objective? I´ve a max power of 3W cw @ 532nm with an
estimated focus at the backfocal plane of ~1mm but I´m not eager to destroy the
objective. Any ideas/advices/experiences how far one can go regarding the laser
excitation?? Many thanks in advance, Steffen |
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Craig Brideau |
Re: Objective - maximum laser power
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Is this CW or pulsed laser power? Pulsed lasers can be more damaging
for an equivalent CW average power. Craig On Thu, Jan 28, 2010 at 2:35 AM, Steffen Steinert <[hidden email]> wrote: > Hello, > > > > I was wondering whether someone tried to focus the excitation laser beam to > the backfocal plane of the objective (60xoil) for widefield illumination. If > so what was the maximum power used without affecting the functionality of > the objective? > > I´ve a max power of 3W cw @ 532nm with an estimated focus at the backfocal > plane of ~1mm but I´m not eager to destroy the objective. Any > ideas/advices/experiences how far one can go regarding the laser > excitation?? > > > > Many thanks in advance, > > > > Steffen > > |
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Steffen Steinert |
AW: Objective - maximum laser power
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Hi Craig,
it´s cw-laser! At a later stage I´m going to run some complicated sequences by shutting off the laser with an AOM, but that´s a different story. At the moment I´d just like to do experiments at cw without damaging the objective. I tried 100mW without any problem (so far), but I´d actually need some more excitation power... Thanks for any input! Steffen -----Ursprüngliche Nachricht----- Von: Confocal Microscopy List [mailto:[hidden email]] Im Auftrag von Craig Brideau Gesendet: Donnerstag, 28. Januar 2010 18:50 An: [hidden email] Betreff: Re: Objective - maximum laser power Is this CW or pulsed laser power? Pulsed lasers can be more damaging for an equivalent CW average power. Craig On Thu, Jan 28, 2010 at 2:35 AM, Steffen Steinert <[hidden email]> wrote: > Hello, > > > > I was wondering whether someone tried to focus the excitation laser beam to > the backfocal plane of the objective (60xoil) for widefield illumination. If > so what was the maximum power used without affecting the functionality of > the objective? > > I´ve a max power of 3W cw @ 532nm with an estimated focus at the backfocal > plane of ~1mm but I´m not eager to destroy the objective. Any > ideas/advices/experiences how far one can go regarding the laser > excitation?? > > > > Many thanks in advance, > > > > Steffen > > |
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Craig Brideau |
Re: Objective - maximum laser power
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In reply to this post by Craig Brideau
Well, I put >400mW of 950nm at 100fs through my microscope with no
trouble, so you should have no problems increasing your power a bit. I could probably even risk a bit more power if I had to although I'd prefer to keep things under 400mW. With CW visible you should be able to do around that. To be careful though you should probably check with the manufacturer if possible. Your mileage may vary, do at own risk, buyer beware, etc. @:-) Check to see if your dichroics and other filtering mechanisms can handle it as they will probably be the most fragile components. Craig On Thu, Jan 28, 2010 at 10:56 AM, Steffen Steinert <[hidden email]> wrote: > Hi Craig, > > it´s cw-laser! At a later stage I´m going to run some complicated sequences > by shutting off the laser with an AOM, but that´s a different story. At the > moment I´d just like to do experiments at cw without damaging the objective. > I tried 100mW without any problem (so far), but I´d actually need some more > excitation power... > > Thanks for any input! > > Steffen > > -----Ursprüngliche Nachricht----- > Von: Confocal Microscopy List [mailto:[hidden email]] Im > Auftrag von Craig Brideau > Gesendet: Donnerstag, 28. Januar 2010 18:50 > An: [hidden email] > Betreff: Re: Objective - maximum laser power > > Is this CW or pulsed laser power? Pulsed lasers can be more damaging > for an equivalent CW average power. > > Craig > > > On Thu, Jan 28, 2010 at 2:35 AM, Steffen Steinert > <[hidden email]> wrote: >> Hello, >> >> >> >> I was wondering whether someone tried to focus the excitation laser beam > to >> the backfocal plane of the objective (60xoil) for widefield illumination. > If >> so what was the maximum power used without affecting the functionality of >> the objective? >> >> I´ve a max power of 3W cw @ 532nm with an estimated focus at the backfocal >> plane of ~1mm but I´m not eager to destroy the objective. Any >> ideas/advices/experiences how far one can go regarding the laser >> excitation?? >> >> >> >> Many thanks in advance, >> >> >> >> Steffen >> >> > |
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In reply to this post by Steffen Steinert
Hello Steffen, We have done 1.2W of
532nm CW through a Zeiss Fluar 40x oil for hours without any appreciable detoriation.
However, we are also a bit apprehensive about the long-term effects on resins
etc in the objective. /Janne Jan Grawé Cell Analysis Core Facility Rudbecklaboratoriet/C5 Dag Hammarskjölds väg 20 SE-75185 Uppsala SWEDEN Phone: +(0)18-4714656 Cell:+(0)70-2577874 From: Confocal Microscopy List
[mailto:[hidden email]] On Behalf Of Steffen Steinert Hello, I was wondering whether someone tried to
focus the excitation laser beam to the backfocal plane of the objective
(60xoil) for widefield illumination. If so what was the maximum power used
without affecting the functionality of the objective? I´ve a max power of 3W cw @ 532nm with an
estimated focus at the backfocal plane of ~1mm but I´m not eager to destroy the
objective. Any ideas/advices/experiences how far one can go regarding the laser
excitation?? Many thanks in advance, Steffen |
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Armstrong, Brian |
Re: Objective - maximum laser power
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Hello, I am just curious, why do you do
this? Brian D Armstrong PhD Light Microscopy Core Manager Beckman Research Institute City of Dept of Neuroscience Duarte, CA 91010 626-256-4673 x62872 http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx From: Hello Steffen, We have done 1.2W of 532nm CW through a
Zeiss Fluar 40x oil for hours without any appreciable detoriation. However, we
are also a bit apprehensive about the long-term effects on resins etc in the
objective. /Janne Jan Grawé Cell Analysis Core
Facility Rudbecklaboratoriet/C5 Dag Hammarskjölds
väg 20 SE-75185 Phone:
+(0)18-4714656 Cell:+(0)70-2577874 From: Hello, I
was wondering whether someone tried to focus the excitation laser beam to the
backfocal plane of the objective (60xoil) for widefield illumination. If so
what was the maximum power used without affecting the functionality of the
objective? I´ve
a max power of 3W cw @ 532nm with an estimated focus at the backfocal plane of
~1mm but I´m not eager to destroy the objective. Any ideas/advices/experiences
how far one can go regarding the laser excitation?? Many
thanks in advance, Steffen
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Steffen Steinert |
AW: Objective - maximum laser power
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Hello,
I am just curious, why do you do this? - Well, I´m actually
using a non-bleachable fluorescent probe, the so called nitrogen-vacancy center
in diamond. Besides other interesting features one can optically
readout magnetic fields. In order to saturate all defect centers in widefield
illumination, it needs a lot of excitation power. Thanks to all who responded,
much appreciated! Best regards, Steffen Von: Confocal Microscopy
List [mailto:[hidden email]] Im Auftrag von Armstrong,
Brian Hello, I am just curious, why do you do this? Brian D Armstrong PhD Light Microscopy Core Manager Beckman Research Institute City of Hope Dept of Neuroscience 1450 E Duarte Rd Duarte, CA 91010 626-256-4673 x62872 http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx From: Confocal Microscopy List
[mailto:[hidden email]] On Behalf Of Jan Grawe Hello Steffen, We have done 1.2W of
532nm CW through a Zeiss Fluar 40x oil for hours without any appreciable
detoriation. However, we are also a bit apprehensive about the long-term
effects on resins etc in the objective. /Janne Jan Grawé Cell Analysis Core Facility Rudbecklaboratoriet/C5 Dag Hammarskjölds väg 20 SE-75185 Uppsala SWEDEN Phone: +(0)18-4714656 Cell:+(0)70-2577874 From: Confocal Microscopy List
[mailto:[hidden email]] On Behalf Of Steffen Steinert Hello, I was wondering whether someone tried to
focus the excitation laser beam to the backfocal plane of the objective
(60xoil) for widefield illumination. If so what was the maximum power used
without affecting the functionality of the objective? I´ve a max power of 3W cw @ 532nm with an
estimated focus at the backfocal plane of ~1mm but I´m not eager to destroy the
objective. Any ideas/advices/experiences how far one can go regarding the laser
excitation?? Many thanks in advance, Steffen
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