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In the end, removing oil from the lens surface is not the real problem -
the key thing is not letting oil get into the interior of the objective.
If you are using an inverted microscope, the best precaution is to
remove the objectives from the nose piece when not in use and store them
tip down, after simple cleaning with lens tissue. Modern oils (unlike
the cedar-wood oil I used as a boy) don't dry up. Your major concern is
getting the oil off the dry objectives, unless you have much better
users than I've ever encountered. Here a piece of expanded polystyrene
is very handy - break it to expose a fresh surface and use it, with firm
pressure, to clean the lens. The freshly cleaved surface is critical.
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
Madsen Building F09, University of Sydney, NSW 2006
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net
-----Original Message-----
From: Confocal Microscopy List [mailto:
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On Behalf Of Suraj Tewari
Sent: Monday, 5 September 2011 7:10 PM
To:
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Subject: Oil protection for objectives on inverted scope for Leica
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I am a using Leica SP5 confocal Microscope,
What are the best way to remove oil from the Objective lenses.
Some one suggested me to use Simply Whatman paper,
Some one said me to use the Ethanol in Whatman paper.
I am interested to know the best way for lens cleaning.
Locked
