Olympus tube-lens with higher magnification

[Shalin Mehta]

Dear Listers,
This is a specific query regarding Olympus microscope optics.

We have a few high-NA low-magnification objectives, for which the
nominal magnification on the objective does not provide sufficient
sampling. Ours is an upright microscope, which makes it somewhat
difficult to add custom magnification on top of the trinocular - as
one needs a mount that mates with Olympus's custom-designed mounting.

So, I am thinking of replacing the default Olympus tube-lens with a
tube-lens of longer focal length (which will lead to increased
magnification above nominal on the objective). Someone informed me
that Olympus does provide such lenses, but I cannot find them on their
website. We have tried adding variable magnification changer part from
Olympus, but that introduces aberrations.

Are such tube-lenses around?

Thanks for any pointers,
Shalin

mobile: [phone]
blog: shalin.wordpress.com

Bioimaging Lab, Block-E3A, #7-10
Div of Bioengineering, NUS Singapore 117574
website: http://www.bioeng.nus.edu.sg/optbioimaging/colin/people.asp#shalinm

[John Oreopoulos]

Could you, as an alternative, switch to a higher magnification objective with approximately the same NA, or switch to a camera with smaller pixel size?

John Oreopoulos, BSc,

PhD Candidate

University of Toronto

Institute For Biomaterials and Biomedical Engineering

Centre For Studies in Molecular Imaging

On 11-Feb-10, at 10:28 PM, Shalin Mehta wrote:

Dear Listers,

This is a specific query regarding Olympus microscope optics.

We have a few high-NA low-magnification objectives, for which the

nominal magnification on the objective does not provide sufficient

sampling. Ours is an upright microscope, which makes it somewhat

difficult to add custom magnification on top of the trinocular - as

one needs a mount that mates with Olympus's custom-designed mounting.

So, I am thinking of replacing the default Olympus tube-lens with a

tube-lens of longer focal length (which will lead to increased

magnification above nominal on the objective). Someone informed me

that Olympus does provide such lenses, but I cannot find them on their

website. We have tried adding variable magnification changer part from

Olympus, but that introduces aberrations.

Are such tube-lenses around?

Thanks for any pointers,

Shalin

mobile: [phone]

blog: shalin.wordpress.com

Bioimaging Lab, Block-E3A, #7-10

Div of Bioengineering, NUS Singapore 117574

website: http://www.bioeng.nus.edu.sg/optbioimaging/colin/people.asp#shalinm

[Mark Cannell]

You can look for achromats in various sizes from Edmond and other
optical supply houses. If you place a suitable divergent lens behind
the  converging  Olympus lens you can increase its FL.

Hope this helps.
 
Cheers

Shalin Mehta wrote:

> Dear Listers,
> This is a specific query regarding Olympus microscope optics.
>
> We have a few high-NA low-magnification objectives, for which the
> nominal magnification on the objective does not provide sufficient
> sampling. Ours is an upright microscope, which makes it somewhat
> difficult to add custom magnification on top of the trinocular - as
> one needs a mount that mates with Olympus's custom-designed mounting.
>
> So, I am thinking of replacing the default Olympus tube-lens with a
> tube-lens of longer focal length (which will lead to increased
> magnification above nominal on the objective). Someone informed me
> that Olympus does provide such lenses, but I cannot find them on their
> website. We have tried adding variable magnification changer part from
> Olympus, but that introduces aberrations.
>
> Are such tube-lenses around?
>
> Thanks for any pointers,
> Shalin
>
> mobile: [phone]
> blog: shalin.wordpress.com
>
> Bioimaging Lab, Block-E3A, #7-10
> Div of Bioengineering, NUS Singapore 117574
> website: http://www.bioeng.nus.edu.sg/optbioimaging/colin/people.asp#shalinm
>  

[Watkins, Simon C]

I would go to optem http://www.qioptiqlinos.com/Products/CameraCouplers/  since they became part of Qioptic their products have been super, particularly if you are doing fluorescence
S

Simon C. Watkins Ph.D, FRC Path
Professor and Vice Chair Cell Biology and Physiology
Professor Immunology
Director Center for Biologic Imaging
BSTS 225
University of Pittsburgh
3500 Terrace St
Pittsburgh PA 15261
412-352-2277
www.cbi.pitt.edu

________________________________________
From: Confocal Microscopy List [[hidden email]] On Behalf Of Shalin Mehta [[hidden email]]
Sent: Thursday, February 11, 2010 10:28 PM
To: [hidden email]
Subject: Olympus tube-lens with higher magnification

Dear Listers,
This is a specific query regarding Olympus microscope optics.

We have a few high-NA low-magnification objectives, for which the
nominal magnification on the objective does not provide sufficient
sampling. Ours is an upright microscope, which makes it somewhat
difficult to add custom magnification on top of the trinocular - as
one needs a mount that mates with Olympus's custom-designed mounting.

So, I am thinking of replacing the default Olympus tube-lens with a
tube-lens of longer focal length (which will lead to increased
magnification above nominal on the objective). Someone informed me
that Olympus does provide such lenses, but I cannot find them on their
website. We have tried adding variable magnification changer part from
Olympus, but that introduces aberrations.

Are such tube-lenses around?

Thanks for any pointers,
Shalin

mobile: [phone]
blog: shalin.wordpress.com

Bioimaging Lab, Block-E3A, #7-10
Div of Bioengineering, NUS Singapore 117574
website: http://www.bioeng.nus.edu.sg/optbioimaging/colin/people.asp#shalinm