Photosensitive, 395nm excitable dye

[Csúcs Gábor-3]

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Dear All,

Linked to a user request, I have a relatively unusual question: instead of a photostable dye, we are looking for an unstable (very sensitive one). The excitation wavelength should be around 395 nm. The user would like to create free radicals in a spatially localized manner (as a result of photobleaching dyes). Does anyone have a suggestion?

Greetings     Gabor

[Craig Brideau]

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Rose Bengal might work. It usually wants green excitation, but might work
with 395nm as well.

Craig

On Wed, Jan 18, 2017 at 10:26 AM, Csúcs Gábor <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
> Linked to a user request, I have a relatively unusual question: instead of
> a photostable dye, we are looking for an unstable (very sensitive one). The
> excitation wavelength should be around 395 nm. The user would like to
> create free radicals in a spatially localized manner (as a result of
> photobleaching dyes). Does anyone have a suggestion?
>
> Greetings     Gabor
>

[mmodel]

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Does it have to be a fluorophore? If not, I would search for photosensitizers

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Craig Brideau
Sent: Wednesday, January 18, 2017 12:53 PM
To: [hidden email]
Subject: Re: Photosensitive, 395nm excitable dye

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Rose Bengal might work. It usually wants green excitation, but might work with 395nm as well.

Craig

On Wed, Jan 18, 2017 at 10:26 AM, Csúcs Gábor <[hidden email]>
wrote:

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> *****
>
> Dear All,
>
> Linked to a user request, I have a relatively unusual question:
> instead of a photostable dye, we are looking for an unstable (very
> sensitive one). The excitation wavelength should be around 395 nm. The
> user would like to create free radicals in a spatially localized
> manner (as a result of photobleaching dyes). Does anyone have a suggestion?
>
> Greetings     Gabor
>

[Iain Johnson]

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If you just want the dye to be photoxic and not fluorescent, a chlorin or
porphyrin-based photosensitizer would likely work well.  These are
compounds optimized and routinely used for photodynamic therapy.  They have
very strong absorption peaks (extinction ~400,000 cm-1 M-1) at about 415
nm.  Foscan (aka Temporfin) is one such compound.  Cayman Chemical sells it
in amounts and at prices suitable for research applications.

Iain

On Wed, Jan 18, 2017 at 9:26 AM, Csúcs Gábor <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
> Linked to a user request, I have a relatively unusual question: instead of
> a photostable dye, we are looking for an unstable (very sensitive one). The
> excitation wavelength should be around 395 nm. The user would like to
> create free radicals in a spatially localized manner (as a result of
> photobleaching dyes). Does anyone have a suggestion?
>
> Greetings     Gabor
>

[jerie]

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Hi Gabor,

Chlorins have an absorption peak at 350-450nm.
DOI: 10.1039/C3PP50376C (Paper) Photochem. Photobiol. Sci., 2014, 13,
1137-1145

Abraços, Jens

Dr. Jens Rietdorf, visiting scientist @ center for technological
development in health CDTS, Oswaldo Cruz Foundation Fiocruz, Rio de Janeiro
Brasil.

On Wed, Jan 18, 2017 at 3:53 PM, Craig Brideau <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Rose Bengal might work. It usually wants green excitation, but might work
> with 395nm as well.
>
> Craig
>
> On Wed, Jan 18, 2017 at 10:26 AM, Csúcs Gábor <[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Dear All,
> >
> > Linked to a user request, I have a relatively unusual question: instead
> of
> > a photostable dye, we are looking for an unstable (very sensitive one).
> The
> > excitation wavelength should be around 395 nm. The user would like to
> > create free radicals in a spatially localized manner (as a result of
> > photobleaching dyes). Does anyone have a suggestion?
> >
> > Greetings     Gabor
> >
>

[George McNamara]

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Hi Gabor,

QDot 655 also makes O2 radicals:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3095390/

Transl Stroke Res.
<https://www.ncbi.nlm.nih.gov/pubmed/?term=A+protocol+for+characterizing+the+impact+of+collateral+flow+after+distal+middle+cerebral+artery+occlusion#>2011
Mar;2(1):112-27. doi: 10.1007/s12975-010-0044-2.


  A*protocol*for*characterizing*the*impact*of*collateral**flow**after**distal**middle**cerebral**artery**occlusion*.

Defazio RA
<https://www.ncbi.nlm.nih.gov/pubmed/?term=Defazio%20RA%5BAuthor%5D&cauthor=true&cauthor_uid=21593993>^1
,Levy S
<https://www.ncbi.nlm.nih.gov/pubmed/?term=Levy%20S%5BAuthor%5D&cauthor=true&cauthor_uid=21593993>,Morales
CL
<https://www.ncbi.nlm.nih.gov/pubmed/?term=Morales%20CL%5BAuthor%5D&cauthor=true&cauthor_uid=21593993>,Levy
RV
<https://www.ncbi.nlm.nih.gov/pubmed/?term=Levy%20RV%5BAuthor%5D&cauthor=true&cauthor_uid=21593993>,Dave
KR
<https://www.ncbi.nlm.nih.gov/pubmed/?term=Dave%20KR%5BAuthor%5D&cauthor=true&cauthor_uid=21593993>,Lin
HW
<https://www.ncbi.nlm.nih.gov/pubmed/?term=Lin%20HW%5BAuthor%5D&cauthor=true&cauthor_uid=21593993>,Abaffy
T
<https://www.ncbi.nlm.nih.gov/pubmed/?term=Abaffy%20T%5BAuthor%5D&cauthor=true&cauthor_uid=21593993>,Watson
BD
<https://www.ncbi.nlm.nih.gov/pubmed/?term=Watson%20BD%5BAuthor%5D&cauthor=true&cauthor_uid=21593993>,Perez-Pinzon
MA
<https://www.ncbi.nlm.nih.gov/pubmed/?term=Perez-Pinzon%20MA%5BAuthor%5D&cauthor=true&cauthor_uid=21593993>,Ohanna
V
<https://www.ncbi.nlm.nih.gov/pubmed/?term=Ohanna%20V%5BAuthor%5D&cauthor=true&cauthor_uid=21593993>.


      Author
      information<https://www.ncbi.nlm.nih.gov/pubmed/?term=A+protocol+for+characterizing+the+impact+of+collateral+flow+after+distal+middle+cerebral+artery+occlusion#>


      Abstract

In humans and in animal models of stroke,*collateral*blood*flow*between
territories of the major pial arteries has a profound*impact*on cortical
infarct size. However, there is a gap in our understanding of the
genetic determinants of*collateral*formation and*flow*, as well as the
signaling pathways and neurovascular interactions regulating this*flow*.
Previous studies have demonstrated that*collateral**flow*between
branches of the anterior*cerebral**artery*(ACA) and
the*middle**cerebral**artery*(MCA) can protect mouse cortex from
infarction*after**middle**cerebral**artery**occlusion*. Because the
number and diameter of collaterals varies among mouse strains
and*after*transgenic manipulations, a combination of methods is required
to control for these variations. Here, we report an inexpensive approach
to*characterizing*the cerebrovascular anatomy, and in vivo monitoring
of*cerebral*blood*flow*as well. Further, we introduce a new, minimally
invasive method for the*occlusion*of*distal*MCA branches. These methods
will permit a new generation of studies on the mechanisms
regulating*collateral*remodeling and cortical blood*flow**after*stroke.

PMID:
    21593993
PMCID:
    PMC3095390 <https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3095390/>
DOI:
    10.1007/s12975-010-0044-2 <https://dx.doi.org/10.1007/s12975-010-0044-2>


On 1/19/2017 12:00 AM, Iain Johnson wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> If you just want the dye to be photoxic and not fluorescent, a chlorin or
> porphyrin-based photosensitizer would likely work well.  These are
> compounds optimized and routinely used for photodynamic therapy.  They have
> very strong absorption peaks (extinction ~400,000 cm-1 M-1) at about 415
> nm.  Foscan (aka Temporfin) is one such compound.  Cayman Chemical sells it
> in amounts and at prices suitable for research applications.
>
> Iain
>
> On Wed, Jan 18, 2017 at 9:26 AM, Csúcs Gábor <[hidden email]>
> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Dear All,
>>
>> Linked to a user request, I have a relatively unusual question: instead of
>> a photostable dye, we are looking for an unstable (very sensitive one). The
>> excitation wavelength should be around 395 nm. The user would like to
>> create free radicals in a spatially localized manner (as a result of
>> photobleaching dyes). Does anyone have a suggestion?
>>
>> Greetings     Gabor
>>

--


George McNamara, PhD
Houston, TX 77054
[hidden email]
https://www.linkedin.com/in/georgemcnamara
https://works.bepress.com/gmcnamara/75/
http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650