Plastic for sample holders for iDISCO+ sample imaging

[Villar Cerviño, Verona]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear list,
Does anyone know what type of plastic can be used for 3D printers to print sample holders that will be used for iDISCO sample imaging in organic media, such as Eci or DBE?

Thanks a lot for your help.
Best regards,

Verona Villar, PhD
Servicio de Imagen
Instituto de Neurociencias CSIC-UMH
E-mail: [hidden email]<mailto:[hidden email]>
Tlf: [phone]
http://in.umh-csic.es/es/si_presentacion.aspx

[Sylvie Le Guyader]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Verona

Silicon works but it is soft so you need a plastic holder.

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Blickagången 16,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: [phone]
LCI website
Follow our microscopy blog!

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Villar Cerviño, Verona
Sent: 25 November 2019 15:36
To: [hidden email]
Subject: Plastic for sample holders for iDISCO+ sample imaging

*****
To join, leave or search the confocal microscopy listserv, go to:
https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C9c4c0dd9c4b44de28c5608d771ba5e84%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C1%7C637102917587891419&amp;sdata=0JrDlMutMM66c4dMOzOtAoynAEuV8m47L6XgcdsGl%2BM%3D&amp;reserved=0
Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C9c4c0dd9c4b44de28c5608d771ba5e84%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C1%7C637102917587891419&amp;sdata=jrnk%2FiiOVU%2FLdosahE28PgpXfYemzfe%2BZ9InCcoM6hI%3D&amp;reserved=0 and include the link in your posting.
*****

Dear list,
Does anyone know what type of plastic can be used for 3D printers to print sample holders that will be used for iDISCO sample imaging in organic media, such as Eci or DBE?

Thanks a lot for your help.
Best regards,

Verona Villar, PhD
Servicio de Imagen
Instituto de Neurociencias CSIC-UMH
E-mail: [hidden email]<mailto:[hidden email]>
Tlf: [phone]
https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fin.umh-csic.es%2Fes%2Fsi_presentacion.aspx&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C9c4c0dd9c4b44de28c5608d771ba5e84%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C1%7C637102917587896416&amp;sdata=hGC74qq5Apvw0OyeIsnx4LX%2B5eTl%2F2UzT4gh5yEJGBE%3D&amp;reserved=0


När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://ki.se/medarbetare/integritetsskyddspolicy>.


Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://ki.se/en/staff/data-protection-policy>.

[Ariel, Pablo]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Verona,

There are several suggestions (some mine, some from other folks) here: https://forum.microlist.org/t/any-experience-with-solvent-resistant-materials-for-sample-holding-in-lightsheet/89 

Happy to discuss more or provide designs/advice off-list.

Best,
Pablo

Pablo Ariel, Ph.D.
Assistant Professor
Director of the Microscopy Services Laboratory
Brinkhous-Bullitt Bldg B04
Department of Pathology and Laboratory Medicine
University of North Carolina at Chapel Hill
http://www.med.unc.edu/microscopy
Tel: [phone]


-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Villar Cerviño, Verona
Sent: Monday, November 25, 2019 9:36 AM
To: [hidden email]
Subject: Plastic for sample holders for iDISCO+ sample imaging

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear list,
Does anyone know what type of plastic can be used for 3D printers to print sample holders that will be used for iDISCO sample imaging in organic media, such as Eci or DBE?

Thanks a lot for your help.
Best regards,

Verona Villar, PhD
Servicio de Imagen
Instituto de Neurociencias CSIC-UMH
E-mail: [hidden email]<mailto:[hidden email]>
Tlf: [phone]
http://in.umh-csic.es/es/si_presentacion.aspx

[Tim Feinstein]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Verona,

We just used Mattek-style glass bottom dishes.  To prevent DBE from eating the plastic and glue, we cut the neck of a volumetric flask into short cylinders with the diamond powder cutting tip on a Dremel tool.  Then we used the same tool to smooth the bottom of the cylinders and secured the cylinder to the dish with superglue.  Any cyanoacrylate glue is compatible with DBE.  If you have a 3D printer and the appropriate plastic type then that may be easier; this was just the fastest route for us.  All the best,


T

Timothy Feinstein, Ph.D.

On 11/25/19, 10:16 AM, "Confocal Microscopy List on behalf of Villar Cerviño, Verona" <[hidden email] on behalf of [hidden email]> wrote:

    *****
    To join, leave or search the confocal microscopy listserv, go to:
    https://nam05.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&amp;data=02%7C01%7Ctnf8%40PITT.EDU%7Cc66d41cee59f498bccbd08d771ba5f00%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C1%7C637102917824265016&amp;sdata=VrIO9Sm7tg4rFQdHAudTXHqqxwS0bLc%2BlfZwZXKo6aQ%3D&amp;reserved=0
    Post images on https://nam05.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&amp;data=02%7C01%7Ctnf8%40PITT.EDU%7Cc66d41cee59f498bccbd08d771ba5f00%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C1%7C637102917824265016&amp;sdata=rliRQPPs9oZs%2F%2Fov36pYkl%2Fzqg6CiXChQe8ZpeP70Fw%3D&amp;reserved=0 and include the link in your posting.
    *****
   
    Dear list,
    Does anyone know what type of plastic can be used for 3D printers to print sample holders that will be used for iDISCO sample imaging in organic media, such as Eci or DBE?
   
    Thanks a lot for your help.
    Best regards,
   
    Verona Villar, PhD
    Servicio de Imagen
    Instituto de Neurociencias CSIC-UMH
    E-mail: [hidden email]<mailto:[hidden email]>
    Tlf: [phone]
    https://nam05.safelinks.protection.outlook.com/?url=http%3A%2F%2Fin.umh-csic.es%2Fes%2Fsi_presentacion.aspx&amp;data=02%7C01%7Ctnf8%40PITT.EDU%7Cc66d41cee59f498bccbd08d771ba5f00%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C1%7C637102917824265016&amp;sdata=FS6JRLzokzKi%2BNv%2BDb0IZoC1M41p2X3uACWVUPTBVaM%3D&amp;reserved=0
   

[Douglas Richardson]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

I haven't had any problems with ECi melting through any plastics to date or
harming any dipping objectives (have not tested it on polystyrene tissue
culture plates). We have converted most of our iDISCO users to ECi rather
than DBE and have not seen any decreases in performance. However, it is a
very limited range of fluorophores that have been tested to date.

BONUS: the facility now has a very pleasant cinnamon smell most days.

-Doug



On Mon, Nov 25, 2019 at 11:44 AM Ariel, Pablo <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Verona,
>
> There are several suggestions (some mine, some from other folks) here:
> https://forum.microlist.org/t/any-experience-with-solvent-resistant-materials-for-sample-holding-in-lightsheet/89
>
> Happy to discuss more or provide designs/advice off-list.
>
> Best,
> Pablo
>
> Pablo Ariel, Ph.D.
> Assistant Professor
> Director of the Microscopy Services Laboratory
> Brinkhous-Bullitt Bldg B04
> Department of Pathology and Laboratory Medicine
> University of North Carolina at Chapel Hill
> http://www.med.unc.edu/microscopy
> Tel: [phone]
>
>
> -----Original Message-----
> From: Confocal Microscopy List <[hidden email]> On
> Behalf Of Villar Cerviño, Verona
> Sent: Monday, November 25, 2019 9:36 AM
> To: [hidden email]
> Subject: Plastic for sample holders for iDISCO+ sample imaging
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear list,
> Does anyone know what type of plastic can be used for 3D printers to print
> sample holders that will be used for iDISCO sample imaging in organic
> media, such as Eci or DBE?
>
> Thanks a lot for your help.
> Best regards,
>
> Verona Villar, PhD
> Servicio de Imagen
> Instituto de Neurociencias CSIC-UMH
> E-mail: [hidden email]<mailto:[hidden email]>
> Tlf: [phone]
> http://in.umh-csic.es/es/si_presentacion.aspx
>

[Sylvie Le Guyader]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Yuck! I don't like that sticky smell! ☹
Yes ECi dissolves polystyrene pretty quick but not polypropylene.

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Blickagången 16,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: [phone]
LCI website
Follow our microscopy blog!

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Douglas Richardson
Sent: 25 November 2019 17:54
To: [hidden email]
Subject: Re: Plastic for sample holders for iDISCO+ sample imaging

*****
To join, leave or search the confocal microscopy listserv, go to:
https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637102976860566468&amp;sdata=JrMUx6l8m%2F3NQGt1T2dXoiOdYo%2FJHt1vb%2Bx%2FIOq5u5M%3D&amp;reserved=0
Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637102976860576462&amp;sdata=Sk0FQnzXK2MIinCPrE3h5VEWdk1%2BSP0X3rHTL1Wt9us%3D&amp;reserved=0 and include the link in your posting.
*****

I haven't had any problems with ECi melting through any plastics to date or harming any dipping objectives (have not tested it on polystyrene tissue culture plates). We have converted most of our iDISCO users to ECi rather than DBE and have not seen any decreases in performance. However, it is a very limited range of fluorophores that have been tested to date.

BONUS: the facility now has a very pleasant cinnamon smell most days.

-Doug



On Mon, Nov 25, 2019 at 11:44 AM Ariel, Pablo <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists
> .umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&amp;data=02%7C01%7Cs
> ylvie.le.guyader%40KI.SE%7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1
> cf4b4f32be3da1dda043c05d%7C0%7C0%7C637102976860576462&amp;sdata=4Bg7x6
> b7dT%2FNCOozNud2VsCT1t96FCBMydt37Z0ko%2FA%3D&amp;reserved=0
> Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637102976860576462&amp;sdata=Sk0FQnzXK2MIinCPrE3h5VEWdk1%2BSP0X3rHTL1Wt9us%3D&amp;reserved=0 and include the link in your posting.
> *****
>
> Hi Verona,
>
> There are several suggestions (some mine, some from other folks) here:
> https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fforu
> m.microlist.org%2Ft%2Fany-experience-with-solvent-resistant-materials-
> for-sample-holding-in-lightsheet%2F89&amp;data=02%7C01%7Csylvie.le.guy
> ader%40KI.SE%7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1cf4b4f32be3d
> a1dda043c05d%7C0%7C0%7C637102976860576462&amp;sdata=yCiLpQlMXTvT1O90Cz
> O9NW3e%2FLW%2FG1BXMjaEecHX80w%3D&amp;reserved=0
>
> Happy to discuss more or provide designs/advice off-list.
>
> Best,
> Pablo
>
> Pablo Ariel, Ph.D.
> Assistant Professor
> Director of the Microscopy Services Laboratory Brinkhous-Bullitt Bldg
> B04 Department of Pathology and Laboratory Medicine University of
> North Carolina at Chapel Hill
> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.m
> ed.unc.edu%2Fmicroscopy&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7
> C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1cf4b4f32be3da1dda043c05d%7
> C0%7C0%7C637102976860576462&amp;sdata=U7VmJYYR39b37RpibkT2QoiBK4t34oaF
> Fj68NEECY4c%3D&amp;reserved=0
> Tel: [phone]
>
>
> -----Original Message-----
> From: Confocal Microscopy List <[hidden email]> On
> Behalf Of Villar Cerviño, Verona
> Sent: Monday, November 25, 2019 9:36 AM
> To: [hidden email]
> Subject: Plastic for sample holders for iDISCO+ sample imaging
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists
> .umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&amp;data=02%7C01%7Cs
> ylvie.le.guyader%40KI.SE%7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1
> cf4b4f32be3da1dda043c05d%7C0%7C0%7C637102976860576462&amp;sdata=4Bg7x6
> b7dT%2FNCOozNud2VsCT1t96FCBMydt37Z0ko%2FA%3D&amp;reserved=0
> Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637102976860576462&amp;sdata=Sk0FQnzXK2MIinCPrE3h5VEWdk1%2BSP0X3rHTL1Wt9us%3D&amp;reserved=0 and include the link in your posting.
> *****
>
> Dear list,
> Does anyone know what type of plastic can be used for 3D printers to
> print sample holders that will be used for iDISCO sample imaging in
> organic media, such as Eci or DBE?
>
> Thanks a lot for your help.
> Best regards,
>
> Verona Villar, PhD
> Servicio de Imagen
> Instituto de Neurociencias CSIC-UMH
> E-mail: [hidden email]<mailto:[hidden email]>
> Tlf: [phone]
> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fin.um
> h-csic.es%2Fes%2Fsi_presentacion.aspx&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637102976860576462&amp;sdata=b2xHZALpnhmv6jYSiYAqruTigNyTLFV%2BkEuaLZgXtgA%3D&amp;reserved=0
>

När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://ki.se/medarbetare/integritetsskyddspolicy>.


Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://ki.se/en/staff/data-protection-policy>.

[Craig Brideau]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

A strategy is to print with cheap plastic, then dip in or paint with a
silicone resin to coat and protect it.

Craig

On Mon, Nov 25, 2019 at 10:00 AM Sylvie Le Guyader <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Yuck! I don't like that sticky smell! ☹
> Yes ECi dissolves polystyrene pretty quick but not polypropylene.
>
> Med vänlig hälsning / Best regards
>
> Sylvie
>
> @@@@@@@@@@@@@@@@@@@@@@@@
> Sylvie Le Guyader, PhD
> Live Cell Imaging Facility Manager
> Karolinska Institutet- Bionut Dpt
> Blickagången 16,
> Room 7362 (lab)/7840 (office)
> 14157 Huddinge, Sweden
> mobile: [phone]
> LCI website
> Follow our microscopy blog!
>
> -----Original Message-----
> From: Confocal Microscopy List <[hidden email]> On
> Behalf Of Douglas Richardson
> Sent: 25 November 2019 17:54
> To: [hidden email]
> Subject: Re: Plastic for sample holders for iDISCO+ sample imaging
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637102976860566468&amp;sdata=JrMUx6l8m%2F3NQGt1T2dXoiOdYo%2FJHt1vb%2Bx%2FIOq5u5M%3D&amp;reserved=0
> Post images on
> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637102976860576462&amp;sdata=Sk0FQnzXK2MIinCPrE3h5VEWdk1%2BSP0X3rHTL1Wt9us%3D&amp;reserved=0
> and include the link in your posting.
> *****
>
> I haven't had any problems with ECi melting through any plastics to date
> or harming any dipping objectives (have not tested it on polystyrene tissue
> culture plates). We have converted most of our iDISCO users to ECi rather
> than DBE and have not seen any decreases in performance. However, it is a
> very limited range of fluorophores that have been tested to date.
>
> BONUS: the facility now has a very pleasant cinnamon smell most days.
>
> -Doug
>
>
>
> On Mon, Nov 25, 2019 at 11:44 AM Ariel, Pablo <[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists
> > .umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&amp;data=02%7C01%7Cs
> > ylvie.le.guyader%40KI.SE%7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1
> > cf4b4f32be3da1dda043c05d%7C0%7C0%7C637102976860576462&amp;sdata=4Bg7x6
> > b7dT%2FNCOozNud2VsCT1t96FCBMydt37Z0ko%2FA%3D&amp;reserved=0
> > Post images on
> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637102976860576462&amp;sdata=Sk0FQnzXK2MIinCPrE3h5VEWdk1%2BSP0X3rHTL1Wt9us%3D&amp;reserved=0
> and include the link in your posting.
> > *****
> >
> > Hi Verona,
> >
> > There are several suggestions (some mine, some from other folks) here:
> > https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fforu
> > m.microlist.org%2Ft%2Fany-experience-with-solvent-resistant-materials-
> > for-sample-holding-in-lightsheet%2F89&amp;data=02%7C01%7Csylvie.le.guy
> > ader%40KI.SE%7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1cf4b4f32be3d
> > a1dda043c05d%7C0%7C0%7C637102976860576462&amp;sdata=yCiLpQlMXTvT1O90Cz
> > O9NW3e%2FLW%2FG1BXMjaEecHX80w%3D&amp;reserved=0
> >
> > Happy to discuss more or provide designs/advice off-list.
> >
> > Best,
> > Pablo
> >
> > Pablo Ariel, Ph.D.
> > Assistant Professor
> > Director of the Microscopy Services Laboratory Brinkhous-Bullitt Bldg
> > B04 Department of Pathology and Laboratory Medicine University of
> > North Carolina at Chapel Hill
> > https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.m
> > ed.unc.edu%2Fmicroscopy&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7
> > C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1cf4b4f32be3da1dda043c05d%7
> > C0%7C0%7C637102976860576462&amp;sdata=U7VmJYYR39b37RpibkT2QoiBK4t34oaF
> > Fj68NEECY4c%3D&amp;reserved=0
> > Tel: [phone]
> >
> >
> > -----Original Message-----
> > From: Confocal Microscopy List <[hidden email]> On
> > Behalf Of Villar Cerviño, Verona
> > Sent: Monday, November 25, 2019 9:36 AM
> > To: [hidden email]
> > Subject: Plastic for sample holders for iDISCO+ sample imaging
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists
> > .umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&amp;data=02%7C01%7Cs
> > ylvie.le.guyader%40KI.SE%7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1
> > cf4b4f32be3da1dda043c05d%7C0%7C0%7C637102976860576462&amp;sdata=4Bg7x6
> > b7dT%2FNCOozNud2VsCT1t96FCBMydt37Z0ko%2FA%3D&amp;reserved=0
> > Post images on
> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637102976860576462&amp;sdata=Sk0FQnzXK2MIinCPrE3h5VEWdk1%2BSP0X3rHTL1Wt9us%3D&amp;reserved=0
> and include the link in your posting.
> > *****
> >
> > Dear list,
> > Does anyone know what type of plastic can be used for 3D printers to
> > print sample holders that will be used for iDISCO sample imaging in
> > organic media, such as Eci or DBE?
> >
> > Thanks a lot for your help.
> > Best regards,
> >
> > Verona Villar, PhD
> > Servicio de Imagen
> > Instituto de Neurociencias CSIC-UMH
> > E-mail: [hidden email]<mailto:[hidden email]>
> > Tlf: [phone]
> > https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fin.um
> > h-csic.es
> %2Fes%2Fsi_presentacion.aspx&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE
> %7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637102976860576462&amp;sdata=b2xHZALpnhmv6jYSiYAqruTigNyTLFV%2BkEuaLZgXtgA%3D&amp;reserved=0
> >
>
>
> När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI
> kommer att behandla dina personuppgifter. Här finns information om hur KI
> behandlar personuppgifter<
> https://ki.se/medarbetare/integritetsskyddspolicy>.
>
>
> Sending email to Karolinska Institutet (KI) will result in KI processing
> your personal data. You can read more about KI’s processing of personal
> data here<https://ki.se/en/staff/data-protection-policy>.
>

[David Elliott]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Verona,

Delrin/PMO filament is compatible with DBE and ECi.

David


---------------------------------------

Dr David Elliott, PhD

HBI AMP Core Microscopy Facility
<http://www.hbi.ucalgary.ca/hbicore>http://www.hbi.ucalgary.ca/hbicore/
University of Calgary
HMRB126, 3330 Hospital Drive, NW
Calgary, AB T2N 4N1
P: 403-210-6661

E: [hidden email]

________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of Craig Brideau <[hidden email]>
Sent: November 25, 2019 10:20 AM
To: [hidden email] <[hidden email]>
Subject: Re: Plastic for sample holders for iDISCO+ sample imaging

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

A strategy is to print with cheap plastic, then dip in or paint with a
silicone resin to coat and protect it.

Craig

On Mon, Nov 25, 2019 at 10:00 AM Sylvie Le Guyader <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Yuck! I don't like that sticky smell! ☹
> Yes ECi dissolves polystyrene pretty quick but not polypropylene.
>
> Med vänlig hälsning / Best regards
>
> Sylvie
>
> @@@@@@@@@@@@@@@@@@@@@@@@
> Sylvie Le Guyader, PhD
> Live Cell Imaging Facility Manager
> Karolinska Institutet- Bionut Dpt
> Blickagången 16,
> Room 7362 (lab)/7840 (office)
> 14157 Huddinge, Sweden
> mobile: [phone]
> LCI website
> Follow our microscopy blog!
>
> -----Original Message-----
> From: Confocal Microscopy List <[hidden email]> On
> Behalf Of Douglas Richardson
> Sent: 25 November 2019 17:54
> To: [hidden email]
> Subject: Re: Plastic for sample holders for iDISCO+ sample imaging
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637102976860566468&amp;sdata=JrMUx6l8m%2F3NQGt1T2dXoiOdYo%2FJHt1vb%2Bx%2FIOq5u5M%3D&amp;reserved=0
> Post images on
> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637102976860576462&amp;sdata=Sk0FQnzXK2MIinCPrE3h5VEWdk1%2BSP0X3rHTL1Wt9us%3D&amp;reserved=0
> and include the link in your posting.
> *****
>
> I haven't had any problems with ECi melting through any plastics to date
> or harming any dipping objectives (have not tested it on polystyrene tissue
> culture plates). We have converted most of our iDISCO users to ECi rather
> than DBE and have not seen any decreases in performance. However, it is a
> very limited range of fluorophores that have been tested to date.
>
> BONUS: the facility now has a very pleasant cinnamon smell most days.
>
> -Doug
>
>
>
> On Mon, Nov 25, 2019 at 11:44 AM Ariel, Pablo <[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists
> > .umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&amp;data=02%7C01%7Cs
> > ylvie.le.guyader%40KI.SE%7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1
> > cf4b4f32be3da1dda043c05d%7C0%7C0%7C637102976860576462&amp;sdata=4Bg7x6
> > b7dT%2FNCOozNud2VsCT1t96FCBMydt37Z0ko%2FA%3D&amp;reserved=0
> > Post images on
> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637102976860576462&amp;sdata=Sk0FQnzXK2MIinCPrE3h5VEWdk1%2BSP0X3rHTL1Wt9us%3D&amp;reserved=0
> and include the link in your posting.
> > *****
> >
> > Hi Verona,
> >
> > There are several suggestions (some mine, some from other folks) here:
> > https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fforu
> > m.microlist.org%2Ft%2Fany-experience-with-solvent-resistant-materials-
> > for-sample-holding-in-lightsheet%2F89&amp;data=02%7C01%7Csylvie.le.guy
> > ader%40KI.SE%7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1cf4b4f32be3d
> > a1dda043c05d%7C0%7C0%7C637102976860576462&amp;sdata=yCiLpQlMXTvT1O90Cz
> > O9NW3e%2FLW%2FG1BXMjaEecHX80w%3D&amp;reserved=0
> >
> > Happy to discuss more or provide designs/advice off-list.
> >
> > Best,
> > Pablo
> >
> > Pablo Ariel, Ph.D.
> > Assistant Professor
> > Director of the Microscopy Services Laboratory Brinkhous-Bullitt Bldg
> > B04 Department of Pathology and Laboratory Medicine University of
> > North Carolina at Chapel Hill
> > https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.m
> > ed.unc.edu%2Fmicroscopy&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7
> > C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1cf4b4f32be3da1dda043c05d%7
> > C0%7C0%7C637102976860576462&amp;sdata=U7VmJYYR39b37RpibkT2QoiBK4t34oaF
> > Fj68NEECY4c%3D&amp;reserved=0
> > Tel: [phone]
> >
> >
> > -----Original Message-----
> > From: Confocal Microscopy List <[hidden email]> On
> > Behalf Of Villar Cerviño, Verona
> > Sent: Monday, November 25, 2019 9:36 AM
> > To: [hidden email]
> > Subject: Plastic for sample holders for iDISCO+ sample imaging
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists
> > .umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&amp;data=02%7C01%7Cs
> > ylvie.le.guyader%40KI.SE%7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1
> > cf4b4f32be3da1dda043c05d%7C0%7C0%7C637102976860576462&amp;sdata=4Bg7x6
> > b7dT%2FNCOozNud2VsCT1t96FCBMydt37Z0ko%2FA%3D&amp;reserved=0
> > Post images on
> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637102976860576462&amp;sdata=Sk0FQnzXK2MIinCPrE3h5VEWdk1%2BSP0X3rHTL1Wt9us%3D&amp;reserved=0
> and include the link in your posting.
> > *****
> >
> > Dear list,
> > Does anyone know what type of plastic can be used for 3D printers to
> > print sample holders that will be used for iDISCO sample imaging in
> > organic media, such as Eci or DBE?
> >
> > Thanks a lot for your help.
> > Best regards,
> >
> > Verona Villar, PhD
> > Servicio de Imagen
> > Instituto de Neurociencias CSIC-UMH
> > E-mail: [hidden email]<mailto:[hidden email]>
> > Tlf: [phone]
> > https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fin.um
> > h-csic.es
> %2Fes%2Fsi_presentacion.aspx&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE
> %7C56d04a278d594cdbd1b908d771c82c4f%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637102976860576462&amp;sdata=b2xHZALpnhmv6jYSiYAqruTigNyTLFV%2BkEuaLZgXtgA%3D&amp;reserved=0
> >
>
>
> När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI
> kommer att behandla dina personuppgifter. Här finns information om hur KI
> behandlar personuppgifter<
> https://ki.se/medarbetare/integritetsskyddspolicy>.
>
>
> Sending email to Karolinska Institutet (KI) will result in KI processing
> your personal data. You can read more about KI’s processing of personal
> data here<https://ki.se/en/staff/data-protection-policy>.
>