Presenting Semi-quantitative Data

[Maria Mazzillo]

Hello,

I have taken photos using fluorescent beads and am using ImageJ to compare
the fluorescence in the sample to the beads.  I have been looking at others
who have presented semi-quantitative data but there is a huge variation in
how it was presented.  I was wondering if anyone has done this kind of thing
before and what the best way is to present the data (graph, table, etc.).

Thanks,

Maria Mazzillo
Auburn University

[mmodel]

Maria - I use a fluorescent field made of a concentrated (10% or so) dye. Then you simply divide your image by the image of a dye and get the intensities. I think it's easier than to measure beads.

Mike Model


Michael Model, Ph.D.
Confocal Microscopy,
Dpt Biological Sciences,
1275 University Esplanade,
Kent State University, Kent, OH 44242
tel. [phone]



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Maria Mazzillo
Sent: Thursday, December 04, 2008 3:40 PM
To: [hidden email]
Subject: Presenting Semi-quantitative Data

Hello,

I have taken photos using fluorescent beads and am using ImageJ to compare
the fluorescence in the sample to the beads.  I have been looking at others
who have presented semi-quantitative data but there is a huge variation in
how it was presented.  I was wondering if anyone has done this kind of thing
before and what the best way is to present the data (graph, table, etc.).

Thanks,

Maria Mazzillo
Auburn University

[Guy Cox]

I usually use those fluorescent plastic slides which Chroma
give away at conferences.  They seem at least as reproducible
as a dye solution and much more convenient.  There is a range of
colours so you can pick one which more or less matches the
fluorochrome you are using.

                                                    Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
    http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone [phone]     Fax [phone]
Mobile [phone]
______________________________________________
 

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of MODEL, MICHAEL
Sent: Friday, 5 December 2008 8:49 AM
To: [hidden email]
Subject: Re: Presenting Semi-quantitative Data

Maria - I use a fluorescent field made of a concentrated (10% or so)
dye. Then you simply divide your image by the image of a dye and get the
intensities. I think it's easier than to measure beads.

Mike Model


Michael Model, Ph.D.
Confocal Microscopy,
Dpt Biological Sciences,
1275 University Esplanade,
Kent State University, Kent, OH 44242
tel. [phone]



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Maria Mazzillo
Sent: Thursday, December 04, 2008 3:40 PM
To: [hidden email]
Subject: Presenting Semi-quantitative Data

Hello,

I have taken photos using fluorescent beads and am using ImageJ to
compare
the fluorescence in the sample to the beads.  I have been looking at
others
who have presented semi-quantitative data but there is a huge variation
in
how it was presented.  I was wondering if anyone has done this kind of
thing
before and what the best way is to present the data (graph, table,
etc.).

Thanks,

Maria Mazzillo
Auburn University

[Peter Zoon]

If you want to do quantitative analysis have a look at
http://www.sipchart.org/.
This method uses coverslides with a carefully calibrated dye doped thin
polymerfilm to get all kinds of quantitative data from you microscope
setup (z-resolution, intensity distribution in xy plane etc.

Peter

Maria Mazzillo wrote:

> Hello,
>
> I have taken photos using fluorescent beads and am using ImageJ to compare
> the fluorescence in the sample to the beads.  I have been looking at others
> who have presented semi-quantitative data but there is a huge variation in
> how it was presented.  I was wondering if anyone has done this kind of thing
> before and what the best way is to present the data (graph, table, etc.).
>
> Thanks,
>
> Maria Mazzillo
> Auburn University
>
>  

--

Peter D. Zoon
Centre for Advanced Microscopy
Section of Molecular Cytology
Swammerdam Institute for Life Sciences
Faculty of Science, University of Amsterdam

Visit.mail: Kruislaan 316 (room 2.03)
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E-mail:     [hidden email]
Tel:        [phone]
Web:        http://wwwmc.bio.uva.nl/
            http://www.science.uva.nl/sils
            http://z00n.net/