Qimaging. Rolara uncooled
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David
I have a customer in Victoria that is purchasing a big confocal system from Nikon with a FN1. He is looking at the Rolara uncooled and was enquiring if there is a demo camera available for sale. We are waiting on the release of the CFI money now so it can happen fairly soon Chris Cathcart ----- Original Message ----- From: David Hitrys <[hidden email]> To: [hidden email] <[hidden email]> Sent: Tue Mar 24 17:41:45 2009 Subject: CCD and EMCCD cameras for ultra-low light microscopy: Educational web session You are invited to attend our live webinar on Friday, 3-April at 1 PM (New York time): "Recent Advancements in CCD and EMCCD Cameras for Ultra-Low Light Microscopy: Technology Overview and Issues to Consider Before Buying." See details below. Admission is free, but connection lines are limited so reserve yours now. -------------------------------------------------------------- TO RESERVE YOUR CONNECTION LINE -------------------------------------------------------------- Complete the information at this link and you will be sent access information for the session: http://www.photomet.com/resources/webinars/webinar_details.php?webinar_id=51 Details: Cameras utilizing Intensification and Electron-Multiplication technologies are used by life and physical science microscopists to enable the imaging of dynamic events under the most challenging low-light conditions such as those encountered with TIRF, FRET, PALM, confocal and deconvolution techniques. Photometrics is presenting a free, live, interactive, web-based seminar on these two signal amplification technologies on Friday, 3-April at 1:00 PM (New York time). Who should attend: Anyone interested in learning about the recent advancements in science-grade CCD cameras for ultra-low light imaging and how these technologies work, what their fundamental limitations are, and what should be considered before acquiring such a camera for their lab should attend. Details are below. There is no cost, but connection lines are limited so reserve yours now. ---------------------------------------- MEETING SUMMARY ---------------------------------------- Name: "Recent Advancements in CCD Cameras for Ultra-Low Light Microscopy: Technology Overview and Issues to Consider Before Buying." Date: Friday, April 3, 2009 Time: 1:00 PM, New York Time Agenda: -Review of Key Concepts: -How Interline CCD Sensors Work -Limitations Imposed by Sensitivity and Noise -Overview of Intensified CCDs -Overview of Electron Multiplied CCDs (EM-CCDs) -Recent Advancements in EM-CCDs -Comparison of Intensified and EM-CCDs -Practical Considerations -Guidance on Selecting a Camera Presented by Photometrics, makers of precision cameras for microscopy, the information imparted will be broadly useful to anyone striving to make the best camera choice for their imaging goals. This session requires attendees to use a Java-enabled browser with a high bandwidth connection. Audio is via toll-free telephone (allows two-way conversation) and computer streaming (only one-way). There is no charge to participate in this on-line seminar. -------------------------------------------------------------- TO RESERVE YOUR CONNECTION LINE -------------------------------------------------------------- Click this URL: http://www.photomet.com/resources/webinars/webinar_details.php?webinar_id=51 ************************************************************************************************** IMPORTANT: The contents of this email and any attachments are confidential. They are intended for the named recipient(s) only. If you have received this email in error, please notify the system manager or the sender immediately and do not disclose the contents to anyone or make copies thereof. *** eSafe scanned this email for viruses, vandals, and malicious content. *** ********************************************************************************************** |
 
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Cathcart, Chris |
Re: Qimaging. Rolara uncooled
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My mistake . Was meant for David only
Chris Cathcart ----- Original Message ----- From: Cathcart, Chris To: '[hidden email]' <[hidden email]> Sent: Tue Mar 24 17:48:58 2009 Subject: Qimaging. Rolara uncooled David I have a customer in Victoria that is purchasing a big confocal system from Nikon with a FN1. He is looking at the Rolara uncooled and was enquiring if there is a demo camera available for sale. We are waiting on the release of the CFI money now so it can happen fairly soon Chris Cathcart ----- Original Message ----- From: David Hitrys <[hidden email]> To: [hidden email] <[hidden email]> Sent: Tue Mar 24 17:41:45 2009 Subject: CCD and EMCCD cameras for ultra-low light microscopy: Educational web session You are invited to attend our live webinar on Friday, 3-April at 1 PM (New York time): "Recent Advancements in CCD and EMCCD Cameras for Ultra-Low Light Microscopy: Technology Overview and Issues to Consider Before Buying." See details below. Admission is free, but connection lines are limited so reserve yours now. -------------------------------------------------------------- TO RESERVE YOUR CONNECTION LINE -------------------------------------------------------------- Complete the information at this link and you will be sent access information for the session: http://www.photomet.com/resources/webinars/webinar_details.php?webinar_id=51 Details: Cameras utilizing Intensification and Electron-Multiplication technologies are used by life and physical science microscopists to enable the imaging of dynamic events under the most challenging low-light conditions such as those encountered with TIRF, FRET, PALM, confocal and deconvolution techniques. Photometrics is presenting a free, live, interactive, web-based seminar on these two signal amplification technologies on Friday, 3-April at 1:00 PM (New York time). Who should attend: Anyone interested in learning about the recent advancements in science-grade CCD cameras for ultra-low light imaging and how these technologies work, what their fundamental limitations are, and what should be considered before acquiring such a camera for their lab should attend. Details are below. There is no cost, but connection lines are limited so reserve yours now. ---------------------------------------- MEETING SUMMARY ---------------------------------------- Name: "Recent Advancements in CCD Cameras for Ultra-Low Light Microscopy: Technology Overview and Issues to Consider Before Buying." Date: Friday, April 3, 2009 Time: 1:00 PM, New York Time Agenda: -Review of Key Concepts: -How Interline CCD Sensors Work -Limitations Imposed by Sensitivity and Noise -Overview of Intensified CCDs -Overview of Electron Multiplied CCDs (EM-CCDs) -Recent Advancements in EM-CCDs -Comparison of Intensified and EM-CCDs -Practical Considerations -Guidance on Selecting a Camera Presented by Photometrics, makers of precision cameras for microscopy, the information imparted will be broadly useful to anyone striving to make the best camera choice for their imaging goals. This session requires attendees to use a Java-enabled browser with a high bandwidth connection. Audio is via toll-free telephone (allows two-way conversation) and computer streaming (only one-way). There is no charge to participate in this on-line seminar. -------------------------------------------------------------- TO RESERVE YOUR CONNECTION LINE -------------------------------------------------------------- Click this URL: http://www.photomet.com/resources/webinars/webinar_details.php?webinar_id=51 ************************************************************************************************** IMPORTANT: The contents of this email and any attachments are confidential. They are intended for the named recipient(s) only. If you have received this email in error, please notify the system manager or the sender immediately and do not disclose the contents to anyone or make copies thereof. *** eSafe scanned this email for viruses, vandals, and malicious content. *** ********************************************************************************************** |
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B. Prabhakar Pandian |
Autofluorescence on cells using FITC filter
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In reply to this post by Cathcart, Chris
Hello,
We are having some issues with imaging of cells using FITC filter for GFP expression. We are seeing that fraction of cells are showing fluorescence when viewed via the FITC filter with no transfection reagent present. We have tried playing with exposure times, different samples, glass, plastic, etc. Can anybody advise. Thanks, -Prabhakar |
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Anda Cornea |
Re: Autofluorescence on cells using FITC filter
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I am going to guess that they will be fluorescent in a TRITC channel as well? Acquire both channels and apply a spectral unmixing algorithm. Or if you just want quick identification of GFP cells display the channels overlay: GFP will be green, the autofluorescent cells will be more yellow, like you would see them though a long pass filter.
Anda Cornea, Ph.D. Director Imaging and Morphology Support Core Oregon National Primate Research Center Oregon Heath & Science University 503-690-5293 -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of B. Prabhakar Pandian Sent: Tuesday, March 24, 2009 3:30 PM To: [hidden email] Subject: Autofluorescence on cells using FITC filter Hello, We are having some issues with imaging of cells using FITC filter for GFP expression. We are seeing that fraction of cells are showing fluorescence when viewed via the FITC filter with no transfection reagent present. We have tried playing with exposure times, different samples, glass, plastic, etc. Can anybody advise. Thanks, -Prabhakar |
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Prabhakar .... How do you know any of your cells are positive for GFP and it isn't all just autofluorescence? Therefore it wouldn't be the scope but your transfection/expression. Could it also be a problem with your prep? Are the cells being imaged live or fixed? Did you also run a control with mock transfection (aka no DNA or better yet GFPless construct but with transfection reagent)?
-----Original Message----- From: Anda Cornea <[hidden email]> Date: Tue, 24 Mar 2009 17:04:34 To: <[hidden email]> Subject: Re: Autofluorescence on cells using FITC filter I am going to guess that they will be fluorescent in a TRITC channel as well? Acquire both channels and apply a spectral unmixing algorithm. Or if you just want quick identification of GFP cells display the channels overlay: GFP will be green, the autofluorescent cells will be more yellow, like you would see them though a long pass filter. Anda Cornea, Ph.D. Director Imaging and Morphology Support Core Oregon National Primate Research Center Oregon Heath & Science University 503-690-5293 -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of B. Prabhakar Pandian Sent: Tuesday, March 24, 2009 3:30 PM To: [hidden email] Subject: Autofluorescence on cells using FITC filter Hello, We are having some issues with imaging of cells using FITC filter for GFP expression. We are seeing that fraction of cells are showing fluorescence when viewed via the FITC filter with no transfection reagent present. We have tried playing with exposure times, different samples, glass, plastic, etc. Can anybody advise. Thanks, -Prabhakar |
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Scott Howell-3 |
Re: Autofluorescence on cells using FITC filter
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In reply to this post by B. Prabhakar Pandian
We had the same problem not long ago in one of the labs scopes. The
emmission filter and the exciter were both delaminated and were letting extra light through. Might be worth checking. Scott J. Howell, Ph.D. Manager, Imaging Module Visual Sciences Research Center Case Western Reserve University 2085 Adelbert Rd. Institute of Pathology Room 106 Cleveland, Ohio 44106 216-368-2300 http://www.case.edu/med/vsrc/ ----- Original Message ----- From: "B. Prabhakar Pandian" <[hidden email]> Date: Tuesday, March 24, 2009 6:34 pm Subject: Autofluorescence on cells using FITC filter To: [hidden email] > Hello, > We are having some issues with imaging of cells using > FITC > filter for GFP expression. We are seeing that fraction of cells are > showing fluorescence when viewed via the FITC filter with no > transfection reagent present. We have tried playing with exposure > times, different samples, glass, plastic, etc. Can anybody advise. > > Thanks, > > -Prabhakar > |
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