Question about LSM510 software
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear all, I am practice FRAP on Zeiss LSM 510-META. Right now I do know how to induce the bleach and record the recovery curve of ROI(by using click "MeanROI" in time series control ). But everytime at the end of recording, only the curves are saved. So my question is can I save the series of image data as well as the curve? Another question is after induce bleach I need to restart the scanning and draw the ROI to measure the mean intensity of them. I always worry about that I might miss the ROI where bleaching happens. Does anyone here know how to do it in a proper way? I really appreciate any help! Thanks, Yi |
 
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leoncio vergara |
Re: Question about LSM510 software
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal I am not sure if there is a way to save both the curve and the images, although it would be nice and useful to see the curve in real time, I usually do the FRAP experiments in our LSM510 meta just saving the images, I look at the curve off line, its very easy to reload the images and use the MeanROI to get the curve from the saved images. regarding savin g the ROI you used for bleaching, there is an option to save the ROI from the ROI editing window. You can always retrieve it later when using the Mean ROI function... just look at the ROI listing. Leoncio ________________________________________ From: Confocal Microscopy List [[hidden email]] On Behalf Of Yi Zheng [[hidden email]] Sent: Wednesday, July 23, 2008 1:39 PM To: [hidden email] Subject: Question about LSM510 software Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear all, I am practice FRAP on Zeiss LSM 510-META. Right now I do know how to induce the bleach and record the recovery curve of ROI(by using click "MeanROI" in time series control ). But everytime at the end of recording, only the curves are saved. So my question is can I save the series of image data as well as the curve? Another question is after induce bleach I need to restart the scanning and draw the ROI to measure the mean intensity of them. I always worry about that I might miss the ROI where bleaching happens. Does anyone here know how to do it in a proper way? I really appreciate any help! Thanks, Yi |
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Rietdorf, Jens |
Re: Question about LSM510 software
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear Yi, You cannot directly save the curve -except you want to work on a screenshot- but you can select to show a table containing the plotted values, these values can be exported to another software to regenerate the graph. There is a 'ROI' folder somewhere in the AIM tree containing the position coordinates of the ROIs you generated. To read data from the bleached region, you can recall the ROI from a list as described by Leoncio. Just as a FRAP aside: be careful with the bleaching power. You can easily achieve powers sufficient to influence molecular interactions with a standard 15mW laser... Cheers, jens -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Vergara, Leoncio A. Sent: Wednesday, July 23, 2008 9:35 PM To: [hidden email] Subject: Re: Question about LSM510 software Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal I am not sure if there is a way to save both the curve and the images, although it would be nice and useful to see the curve in real time, I usually do the FRAP experiments in our LSM510 meta just saving the images, I look at the curve off line, its very easy to reload the images and use the MeanROI to get the curve from the saved images. regarding savin g the ROI you used for bleaching, there is an option to save the ROI from the ROI editing window. You can always retrieve it later when using the Mean ROI function... just look at the ROI listing. Leoncio ________________________________________ From: Confocal Microscopy List [[hidden email]] On Behalf Of Yi Zheng [[hidden email]] Sent: Wednesday, July 23, 2008 1:39 PM To: [hidden email] Subject: Question about LSM510 software Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear all, I am practice FRAP on Zeiss LSM 510-META. Right now I do know how to induce the bleach and record the recovery curve of ROI(by using click "MeanROI" in time series control ). But everytime at the end of recording, only the curves are saved. So my question is can I save the series of image data as well as the curve? Another question is after induce bleach I need to restart the scanning and draw the ROI to measure the mean intensity of them. I always worry about that I might miss the ROI where bleaching happens. Does anyone here know how to do it in a proper way? I really appreciate any help! Thanks, Yi |
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Chen, Mei Ling |
Re: Question about LSM510 software
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal You can not save the curve directly but you can export it and save the curve. ML At 01:23 PM 7/24/08, you wrote: >Search the CONFOCAL archive at >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >Dear Yi, > >You cannot directly save the curve -except you want to work on a >screenshot- but you can select to show a table containing the plotted >values, these values can be exported to another software to regenerate >the graph. > >There is a 'ROI' folder somewhere in the AIM tree containing the >position coordinates of the ROIs you generated. To read data from the >bleached region, you can recall the ROI from a list as described by >Leoncio. > >Just as a FRAP aside: be careful with the bleaching power. You can >easily achieve powers sufficient to influence molecular interactions >with a standard 15mW laser... > >Cheers, jens > >-----Original Message----- >From: Confocal Microscopy List [mailto:[hidden email]] On >Behalf Of Vergara, Leoncio A. >Sent: Wednesday, July 23, 2008 9:35 PM >To: [hidden email] >Subject: Re: Question about LSM510 software > >Search the CONFOCAL archive at >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >I am not sure if there is a way to save both the curve and the images, >although it would be nice and useful to see the curve in real time, I >usually do the FRAP experiments in our LSM510 meta just saving the >images, I look at the curve off line, its very easy to reload the images >and use the MeanROI to get the curve from the saved images. > >regarding savin g the ROI you used for bleaching, there is an option to >save the ROI from the ROI editing window. You can always retrieve it >later when using the Mean ROI function... just look at the ROI listing. > >Leoncio > > > >________________________________________ >From: Confocal Microscopy List [[hidden email]] On Behalf >Of Yi Zheng [[hidden email]] >Sent: Wednesday, July 23, 2008 1:39 PM >To: [hidden email] >Subject: Question about LSM510 software > >Search the CONFOCAL archive at >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >Dear all, > >I am practice FRAP on Zeiss LSM 510-META. > >Right now I do know how to induce the bleach and record the recovery >curve >of ROI(by using click "MeanROI" in time series control ). But everytime >at the >end of recording, only the curves are saved. So my question is can I >save the >series of image data as well as the curve? > >Another question is after induce bleach I need to restart the scanning >and >draw the ROI to measure the mean intensity of them. I always worry about >that I might miss the ROI where bleaching happens. Does anyone here know >how to do it in a proper way? > >I really appreciate any help! > >Thanks, > > >Yi |
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vaishali kailaje |
Re: Question about LSM510 software
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In reply to this post by yee z
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi At the end of your FRAP exp. you have a series of images when you go to gallery mode you can see all of them i.e. a pre-bleach, bleach and post bleach images.The recovery curve and the image series can be exported into tiff fromat seperately and then saved so that you get both the images. When you save an image with ROI it will show only that ROI in the image so you should be able to do your intensity measurememtns.Be careful while starting a fresh exp. you have to delete the exsisting ROI in the 'DEFINE REGION option of the Bleach Control and add a new one by giving it a diffrent name.If you do not delete you will get the previous ROI as well in your image. The procedure for carrying out FRAP is mentioned in the help menu as 'FRAP GUIDE'provided you have the analysis software available i.e KINETIC'. In short, -Set the appropriate configurations as well as scan parameters. -Go to edit bleach option and do appropriate settings. -Define regions to be bleached. -Set a time series by giving the time intervals and number of images. -Say Start B. You could just try the above. Best luck. Vaishali Kailaje Tata Memorial Centre, Advancd Centre for Treatment and Research in Cancer, Kharghar, Navi Mumbai, India. --- On Thu, 7/24/08, Yi Zheng <[hidden email]> wrote: > From: Yi Zheng <[hidden email]> > Subject: Question about LSM510 software > To: [hidden email] > Date: Thursday, July 24, 2008, 12:09 AM > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > Dear all, > > I am practice FRAP on Zeiss LSM 510-META. > > Right now I do know how to induce the bleach and record the > recovery curve > of ROI(by using click "MeanROI" in time series > control ). But everytime at the > end of recording, only the curves are saved. So my question > is can I save the > series of image data as well as the curve? > > Another question is after induce bleach I need to restart > the scanning and > draw the ROI to measure the mean intensity of them. I > always worry about > that I might miss the ROI where bleaching happens. Does > anyone here know > how to do it in a proper way? > > I really appreciate any help! > > Thanks, > > > Yi |
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Holly L. AARON |
Re: Question about LSM510 software
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In reply to this post by yee z
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi, Yi - there is a well-hidden setting in the Options --> Settings Menu. Look for "Scan Mean of ROIs" - there are check boxes for scanning and saving the entire image. In the newer versions of the software, the ROI should be saved with the image. Otherwise, retrieve acc to Leoncio's advise. Best, Holly __________________ Holly L. Aaron CRL Molecular Imaging Center http://imaging.berkeley.edu -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Yi Zheng Sent: Wednesday, July 23, 2008 11:39 AM To: [hidden email] Subject: Question about LSM510 software Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear all, I am practice FRAP on Zeiss LSM 510-META. Right now I do know how to induce the bleach and record the recovery curve of ROI(by using click "MeanROI" in time series control ). But everytime at the end of recording, only the curves are saved. So my question is can I save the series of image data as well as the curve? Another question is after induce bleach I need to restart the scanning and draw the ROI to measure the mean intensity of them. I always worry about that I might miss the ROI where bleaching happens. Does anyone here know how to do it in a proper way? I really appreciate any help! Thanks, Yi |
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In reply to this post by Rietdorf, Jens
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
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