Raman peaks in Water / Cells

[Wolf Heusermann-2]

Hi all,

I do have another question. This one concerning the raman peaks.
Does anyone have an idea where to expect the raman peak -  imaging a cell
with the following laser lines: 405,485, 532, 640, 730nm.
I found a measured value for 485nm at 580nm in Water. How good does this
correspond to the cell?

Thank you very much in advance for any info you can provide.

Best regards,

Wolf




   

[Johnson, Iain-2]

The Raman shift of water is a fixed frequency of 3380 cm-1 relative to the excitation wavelength

ex Raman
405 469
485 580
532 648
640 817
730 969

The other thing to remember is that the Raman intensity goes as the 4th power of frequency, so declines very rapidly as you go through the visible and into the near IR.

Iain




-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Wolf Heusermann
Sent: Tuesday, February 09, 2010 9:22 AM
To: [hidden email]
Subject: Raman peaks in Water / Cells

Hi all,

I do have another question. This one concerning the raman peaks.
Does anyone have an idea where to expect the raman peak -  imaging a cell
with the following laser lines: 405,485, 532, 640, 730nm.
I found a measured value for 485nm at 580nm in Water. How good does this
correspond to the cell?

Thank you very much in advance for any info you can provide.

Best regards,

Wolf




   
 

[Andreas Bruckbauer]

That depends what molecule and vibration you want to look at, for water (you will have a lot of it in a cell) you have a broad band around 3100 - 3600 cm^-1 from OH. When you take 3300 cm-1 you will get 577 nm for the stokes shift when exciting at 485 nm. You have to calculate 10^7/(1/485 * 1e7 - 3300), the same for the other wavelengths. I managed to see this in a confocal with the spectral detector using very slow scan speeds when focussing directly into the water, it was the FV1000 from Olympus. Not very useful for imaging though unless you do CARS or stimulated emission.
Andreas

-----Original Message-----
From: Wolf Heusermann <[hidden email]>
To: [hidden email]
Sent: Tue, 9 Feb 2010 17:21
Subject: Raman peaks in Water / Cells

Hi all,



I do have another question. This one concerning the raman peaks.

Does anyone have an idea where to expect the raman peak -  imaging a cell

with the following laser lines: 405,485, 532, 640, 730nm.

I found a measured value for 485nm at 580nm in Water. How good does this

correspond to the cell? 



Thank you very much in advance for any info you can provide.



Best regards,



Wolf