Re: FRAP curve T1/2 calculation

[Axel Kurt Preuss]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****


Hi everybody
Would anybody know a freeware or online software tool, simple to use, which calculates the half time of FRAP curves

Thanks
Axel


Note: This message may contain confidential information. If this Email/Fax has been sent to you by mistake, please notify the sender and delete it immediately. Thank you.

[John Oreopoulos]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi Axel,

There seem to be a number of options at your disposal that are based out of ImageJ. Try looking here:

http://imagejdocu.tudor.lu/doku.php?id=plugin:analysis:frap_analysis:start

and here (at the bottom):

http://www.macbiophotonics.ca/imagej/intensity_vs_time_ana.htm

There's even a nice "how to" JOVE video:

http://www.jove.com/video/2568/fluorescence-recovery-after-photobleaching-frap-of-fluorescence-tagged-proteins-in-dendritic-spines-of-cultured-hippocampal-neurons

It seems to me that even ImageJ's basic intensity vs time tools and the built in curve fitter could be used to construct a simple and customizable FRAP analysis tool for your particular sample type if you wanted.

Cheers

John Oreopoulos
Research Assistant
Spectral Applied Research
Richmond Hill, Ontario
Canada
www.spectral.ca


On 2011-11-22, at 4:14 AM, Axel Kurt Preuss (IMCB) wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
>
> Hi everybody
> Would anybody know a freeware or online software tool, simple to use, which calculates the half time of FRAP curves
>
> Thanks
> Axel
>
>
> Note: This message may contain confidential information. If this Email/Fax has been sent to you by mistake, please notify the sender and delete it immediately. Thank you.

[Bonnaud, Fabien]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi Axel,

Hope you're doing well !

PerkinElmer cellular imaging are pleased to announce that Volocity 6.0 also offers offline FRAP analysis.

It will give you t 1/2 from different recovery models from all images formats, through a simple and fast workflow.

www.cellularimaging.com

Should you need information don't hesitate to contact us.

Cheers

Fabien


Fabien Bonnaud
Imaging & Analysis Specialist, Imaging & Detection Technologies

PerkinElmer
 



----- Original Message -----
From: Axel Kurt Preuss (IMCB) [mailto:[hidden email]]
Sent: Tuesday, November 22, 2011 09:14 AM
To: [hidden email] <[hidden email]>
Subject: Re: FRAP curve T1/2 calculation

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****


Hi everybody
Would anybody know a freeware or online software tool, simple to use, which calculates the half time of FRAP curves

Thanks
Axel


Note: This message may contain confidential information. If this Email/Fax has been sent to you by mistake, please notify the sender and delete it immediately. Thank you.

[Vladimir Ghukasyan-2]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi Axel,

We tried several methods and the one developed by Dr. Kota Miura as a
script for IgorPro worked the best for us. It has a very nice
algorithm compensating for general photobleaching (outside of the ROI
of interest), has more flexibility in fitting parameters, offers three
different ways to fit the data.
The procedure file with the detailed introduction can be found here:
http://cmci.embl.de/downloads/frap_analysis

Best wishes,
Vladimir


===============
Vladimir Ghukasyan
Confocal and Multiphoton Imaging Facility
Neuroscience Center

115 Mason Farm Rd., Bld. 245, Rm. 7109F
Chapel Hill 27599-7250
NC

Tel.: [phone]
Fax: [phone]

On Tue, Nov 22, 2011 at 4:14 AM, Axel Kurt Preuss (IMCB)
<[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
>
> Hi everybody
> Would anybody know a freeware or online software tool, simple to use, which calculates the half time of FRAP curves
>
> Thanks
> Axel
>
>
> Note: This message may contain confidential information. If this Email/Fax has been sent to you by mistake, please notify the sender and delete it immediately. Thank you.
>