Removable glue for optics
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear all, I need to glue some glass optics onto anodized aluminum surfaces (edges of coated dichroic mirror surface and of a full reflective silver mirror). The problem is that I'd like to be able to remove the mirrors at some point without completely ruining their reflective surfaces and leaving too much residue on the glass and aluminum. The glued parts are not in the optical path so the glue does not have to be transparent. Also the glue can be quite weak, just enough so that the mirrors don't fall off. Can anyone suggest a nice glue for that? Best, Tobias |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Three thoughts: PVA blobs which can be peeled after setting or hot glue (stronger -harder to peel). Nail varnish is good too. I've used all 3 but YMMV depending on area of contact etc. HTH Mark On 25/06/2012, at 4:38 PM, Tobias Rose wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear all, > > I need to glue some glass optics onto anodized aluminum surfaces (edges of coated dichroic mirror surface and of a full reflective silver mirror). The problem is that I'd like to be able to remove the mirrors at some point without completely ruining their reflective surfaces and leaving too much residue on the glass and aluminum. > The glued parts are not in the optical path so the glue does not have to be transparent. Also the glue can be quite weak, just enough so that the mirrors don't fall off. > > Can anyone suggest a nice glue for that? > > Best, > Tobias |
 
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Matthew Nicholas-2 |
Re: Removable glue for optics
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In reply to this post by Tobias Rose
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Tobias, We've had good luck with 732 RTV "multipurpose sealant" from Dow. Ours is clear, but I believe they also make it in black. This was suggested to us by Chroma Technology. It is basically like a silicone sealant, but can be used in small amount to do exactly what you want. When it dries, it keeps the parts secure, but still has a little 'give' so that it does not induce strain in the mirror that could deform the surface (this was a major problem for us using other hard-drying glues, and lead to awful-looking interference patterns in profiles of lasers reflected off the distorted surfaces of the mirrors). If you need to get the component off later, it is relatively easy using a scalpel, and any residue can be carefully removed with ethanol and a Q-tip (I try to keep the glue entirely off the optical surface, however). Best of luck, Matt On 6/25/12 11:38 AM, Tobias Rose wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear all, > > I need to glue some glass optics onto anodized aluminum surfaces (edges of coated dichroic mirror surface and of a full reflective silver mirror). The problem is that I'd like to be able to remove the mirrors at some point without completely ruining their reflective surfaces and leaving too much residue on the glass and aluminum. > The glued parts are not in the optical path so the glue does not have to be transparent. Also the glue can be quite weak, just enough so that the mirrors don't fall off. > > Can anyone suggest a nice glue for that? > > Best, > Tobias -- Matthew Nicholas Medical Scientist Training Program Student Laboratory of Arne Gennerich Department of Anatomy and Structural Biology Albert Einstein College of Medicine Forchheimer Building, Room 628 1300 Morris Park Avenue Bronx, New York 10461 718.430.3446 [hidden email] |
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In reply to this post by Mark Cannell
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** rubber cement, two sided scotch tape, paraffin, dental wax, postit note folded so adhesive is on both sides, gelatin.... On Mon, Jun 25, 2012 at 10:45 AM, Mark Cannell <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Three thoughts: > > PVA blobs which can be peeled after setting or hot glue (stronger -harder > to peel). Nail varnish is good too. I've used all 3 but YMMV depending on > area of contact etc. > > HTH > > Mark > > > On 25/06/2012, at 4:38 PM, Tobias Rose wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > Dear all, > > > > I need to glue some glass optics onto anodized aluminum surfaces (edges > of coated dichroic mirror surface and of a full reflective silver mirror). > The problem is that I'd like to be able to remove the mirrors at some point > without completely ruining their reflective surfaces and leaving too much > residue on the glass and aluminum. > > The glued parts are not in the optical path so the glue does not have to > be transparent. Also the glue can be quite weak, just enough so that the > mirrors don't fall off. > > > > Can anyone suggest a nice glue for that? > > > > Best, > > Tobias > -- Michael J. Herron, U of MN, Dept. of Entomology [hidden email] 612-624-3688 (office) 612-625-5299 (FAX) |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Depending on the hold strength you need, silicone works fairly well and can be peeled off cleanly later. Craig On Mon, Jun 25, 2012 at 12:20 PM, Michael Herron <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > rubber cement, two sided scotch tape, paraffin, dental wax, postit note > folded so adhesive is on both sides, gelatin.... > > On Mon, Jun 25, 2012 at 10:45 AM, Mark Cannell <[hidden email] > >wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > Three thoughts: > > > > PVA blobs which can be peeled after setting or hot glue (stronger -harder > > to peel). Nail varnish is good too. I've used all 3 but YMMV depending > on > > area of contact etc. > > > > HTH > > > > Mark > > > > > > On 25/06/2012, at 4:38 PM, Tobias Rose wrote: > > > > > ***** > > > To join, leave or search the confocal microscopy listserv, go to: > > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > > ***** > > > > > > Dear all, > > > > > > I need to glue some glass optics onto anodized aluminum surfaces (edges > > of coated dichroic mirror surface and of a full reflective silver > mirror). > > The problem is that I'd like to be able to remove the mirrors at some > point > > without completely ruining their reflective surfaces and leaving too much > > residue on the glass and aluminum. > > > The glued parts are not in the optical path so the glue does not have > to > > be transparent. Also the glue can be quite weak, just enough so that the > > mirrors don't fall off. > > > > > > Can anyone suggest a nice glue for that? > > > > > > Best, > > > Tobias > > > > > > -- > Michael J. Herron, U of MN, Dept. of Entomology > [hidden email] > 612-624-3688 (office) 612-625-5299 (FAX) > |
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In reply to this post by Michael Herron
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Duco cement will set up under a heat lamp and we coat multi layer thin film coatings on set up plates for 100,12.5mm glass substrates all the time. Let the Duco set up and releive any outgassing, clean up is as easy as Acetone to dissolve and clean the surface. Get a syringe and just touch the edge between the placed substrate and the aluminum and leave a small dab in the corners. If they have to spin put a little more in the middles just tack it into place. Gregg On 6/25/12, Michael Herron <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > rubber cement, two sided scotch tape, paraffin, dental wax, postit note > folded so adhesive is on both sides, gelatin.... > > On Mon, Jun 25, 2012 at 10:45 AM, Mark Cannell > <[hidden email]>wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Three thoughts: >> >> PVA blobs which can be peeled after setting or hot glue (stronger -harder >> to peel). Nail varnish is good too. I've used all 3 but YMMV depending >> on >> area of contact etc. >> >> HTH >> >> Mark >> >> >> On 25/06/2012, at 4:38 PM, Tobias Rose wrote: >> >> > ***** >> > To join, leave or search the confocal microscopy listserv, go to: >> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> > ***** >> > >> > Dear all, >> > >> > I need to glue some glass optics onto anodized aluminum surfaces (edges >> of coated dichroic mirror surface and of a full reflective silver >> mirror). >> The problem is that I'd like to be able to remove the mirrors at some >> point >> without completely ruining their reflective surfaces and leaving too much >> residue on the glass and aluminum. >> > The glued parts are not in the optical path so the glue does not have >> > to >> be transparent. Also the glue can be quite weak, just enough so that the >> mirrors don't fall off. >> > >> > Can anyone suggest a nice glue for that? >> > >> > Best, >> > Tobias >> > > > > -- > Michael J. Herron, U of MN, Dept. of Entomology > [hidden email] > 612-624-3688 (office) 612-625-5299 (FAX) > -- Gregg Jarvis Advanced Products Group Omega Optical Inc. 24 Omega Drive Brattleboro, VT 05301 [hidden email] 1-802-251-7316 |
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In reply to this post by Craig Brideau
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Crazy Glue. It's really thin. To remove, squirt on solvent or dunk in solvent. ________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270 -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Craig Brideau Sent: Monday, June 25, 2012 2:42 PM To: [hidden email] Subject: Re: Removable glue for optics ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Depending on the hold strength you need, silicone works fairly well and can be peeled off cleanly later. Craig On Mon, Jun 25, 2012 at 12:20 PM, Michael Herron <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > rubber cement, two sided scotch tape, paraffin, dental wax, postit > note folded so adhesive is on both sides, gelatin.... > > On Mon, Jun 25, 2012 at 10:45 AM, Mark Cannell > <[hidden email] > >wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > Three thoughts: > > > > PVA blobs which can be peeled after setting or hot glue (stronger > > -harder to peel). Nail varnish is good too. I've used all 3 but > > YMMV depending > on > > area of contact etc. > > > > HTH > > > > Mark > > > > > > On 25/06/2012, at 4:38 PM, Tobias Rose wrote: > > > > > ***** > > > To join, leave or search the confocal microscopy listserv, go to: > > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > > ***** > > > > > > Dear all, > > > > > > I need to glue some glass optics onto anodized aluminum surfaces > > > (edges > > of coated dichroic mirror surface and of a full reflective silver > mirror). > > The problem is that I'd like to be able to remove the mirrors at > > some > point > > without completely ruining their reflective surfaces and leaving too > > much residue on the glass and aluminum. > > > The glued parts are not in the optical path so the glue does not > > > have > to > > be transparent. Also the glue can be quite weak, just enough so that > > the mirrors don't fall off. > > > > > > Can anyone suggest a nice glue for that? > > > > > > Best, > > > Tobias > > > > > > -- > Michael J. Herron, U of MN, Dept. of Entomology [hidden email] > 612-624-3688 (office) 612-625-5299 (FAX) > |
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In reply to this post by Tobias Rose
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Tobias This is old but it works for temporary bonds. Collodian. Dries fast, light to medium hold, dissolves easily and most importantly, cheap! Caution, flammable when in liquid form. You might find it in the OR of a hospital. Dan On Jun 25, 2012, at 11:38 AM, Tobias Rose wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear all, > > I need to glue some glass optics onto anodized aluminum surfaces > (edges of coated dichroic mirror surface and of a full reflective > silver mirror). The problem is that I'd like to be able to remove > the mirrors at some point without completely ruining their > reflective surfaces and leaving too much residue on the glass and > aluminum. > The glued parts are not in the optical path so the glue does not > have to be transparent. Also the glue can be quite weak, just > enough so that the mirrors don't fall off. > > Can anyone suggest a nice glue for that? > > Best, > Tobias Dan Focht [hidden email] Bioptechs, Inc. 3560 Beck Rd. Butler, PA 16002 www.bioptechs.com Direct 724-282-7145 Fax 724-282-0745 Toll Free 877 LIVE-CELL - (548-3235) |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** He everybody, Thanks a lot for all the answers - very useful! I am a bit reluctant to rely on solvents like acetone to remove the glue, though. Does anyone know if the surface coating of standard chroma dichroics would survive acetone treatment? I think I will go with silicone in the corners and edges of the dichroic / aluminum interface for now. I just hope it's not getting sucked under the glass... Just to sum up the responses: Collodian (wow - that stuff is ancient!) Crazy Glue Duco cement silicone (e.g 732 RTV) rubber cement two sided scotch tape paraffin dental wax postit note gelatin PVA blobs Hot glue Nail varnish Best, Tobias -- Tobias Rose, PhD Max-Planck-Institute of Neurobiology Department of Cellular and Systems Neurobiology Am Klopferspitz 18 D-82152 Martinsried Phone: +49 89 8578 - 3684 Fax: +49 89 8578 - 2481 [hidden email] http://www.neuro.mpg.de/37634/rose > -----Original Message----- > From: Confocal Microscopy List > [mailto:[hidden email]] On Behalf Of Dan Focht > Sent: Tuesday, June 26, 2012 8:26 AM > To: [hidden email] > Subject: Re: Removable glue for optics > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Tobias > > > This is old but it works for temporary bonds. > Collodian. > > Dries fast, light to medium hold, dissolves easily and most importantly, > cheap! > Caution, flammable when in liquid form. > You might find it in the OR of a hospital. > > > Dan > > > On Jun 25, 2012, at 11:38 AM, Tobias Rose wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > Dear all, > > > > I need to glue some glass optics onto anodized aluminum surfaces > > (edges of coated dichroic mirror surface and of a full reflective > > silver mirror). The problem is that I'd like to be able to remove the > > mirrors at some point without completely ruining their reflective > > surfaces and leaving too much residue on the glass and aluminum. > > The glued parts are not in the optical path so the glue does not have > > to be transparent. Also the glue can be quite weak, just enough so > > that the mirrors don't fall off. > > > > Can anyone suggest a nice glue for that? > > > > Best, > > Tobias > > Dan Focht > [hidden email] > Bioptechs, Inc. > 3560 Beck Rd. > Butler, PA 16002 > www.bioptechs.com > Direct 724-282-7145 > Fax 724-282-0745 > Toll Free 877 LIVE-CELL - (548-3235) |
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Zac Arrac Atelaz |
Re: Removable glue for optics
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** There are layers in new lenses that can not stand organic solvents as Acetone, be careful with that, you might soak a color filter, and end up with transparent glass, better ask them about the model you purchased. Best regards Gabriel OH > Date: Tue, 26 Jun 2012 14:24:33 +0000 > From: [hidden email] > Subject: Re: Removable glue for optics > To: [hidden email] > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > He everybody, > > Thanks a lot for all the answers - very useful! > > I am a bit reluctant to rely on solvents like acetone to remove the glue, though. Does anyone know if the surface coating of standard chroma dichroics would survive acetone treatment? > > I think I will go with silicone in the corners and edges of the dichroic / aluminum interface for now. I just hope it's not getting sucked under the glass... > > Just to sum up the responses: > > Collodian (wow - that stuff is ancient!) > Crazy Glue > Duco cement > silicone (e.g 732 RTV) > rubber cement > two sided scotch tape > paraffin > dental wax > postit note > gelatin > PVA blobs > Hot glue > Nail varnish > > Best, > Tobias > > -- > Tobias Rose, PhD > Max-Planck-Institute of Neurobiology > Department of Cellular and Systems Neurobiology > Am Klopferspitz 18 > D-82152 Martinsried > Phone: +49 89 8578 - 3684 > Fax: +49 89 8578 - 2481 > [hidden email] > http://www.neuro.mpg.de/37634/rose > > > > -----Original Message----- > > From: Confocal Microscopy List > > [mailto:[hidden email]] On Behalf Of Dan Focht > > Sent: Tuesday, June 26, 2012 8:26 AM > > To: [hidden email] > > Subject: Re: Removable glue for optics > > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > Tobias > > > > > > This is old but it works for temporary bonds. > > Collodian. > > > > Dries fast, light to medium hold, dissolves easily and most importantly, > > cheap! > > Caution, flammable when in liquid form. > > You might find it in the OR of a hospital. > > > > > > Dan > > > > > > On Jun 25, 2012, at 11:38 AM, Tobias Rose wrote: > > > > > ***** > > > To join, leave or search the confocal microscopy listserv, go to: > > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > > ***** > > > > > > Dear all, > > > > > > I need to glue some glass optics onto anodized aluminum surfaces > > > (edges of coated dichroic mirror surface and of a full reflective > > > silver mirror). The problem is that I'd like to be able to remove the > > > mirrors at some point without completely ruining their reflective > > > surfaces and leaving too much residue on the glass and aluminum. > > > The glued parts are not in the optical path so the glue does not have > > > to be transparent. Also the glue can be quite weak, just enough so > > > that the mirrors don't fall off. > > > > > > Can anyone suggest a nice glue for that? > > > > > > Best, > > > Tobias > > > > Dan Focht > > [hidden email] > > Bioptechs, Inc. > > 3560 Beck Rd. > > Butler, PA 16002 > > www.bioptechs.com > > Direct 724-282-7145 > > Fax 724-282-0745 > > Toll Free 877 LIVE-CELL - (548-3235) |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Chroma dichroics should be hard oxide or sputtered, should be no problem for solvents. On 6/26/12, Zac Arrac Atelaz <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > > There are layers in new lenses that can not stand organic solvents as > Acetone, be careful with that, you might soak a color filter, and end up > with transparent glass, better ask them about the model you purchased. Best > regards Gabriel OH > > Date: Tue, 26 Jun 2012 14:24:33 +0000 >> From: [hidden email] >> Subject: Re: Removable glue for optics >> To: [hidden email] >> >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> He everybody, >> >> Thanks a lot for all the answers - very useful! >> >> I am a bit reluctant to rely on solvents like acetone to remove the glue, >> though. Does anyone know if the surface coating of standard chroma >> dichroics would survive acetone treatment? >> >> I think I will go with silicone in the corners and edges of the dichroic / >> aluminum interface for now. I just hope it's not getting sucked under the >> glass... >> >> Just to sum up the responses: >> >> Collodian (wow - that stuff is ancient!) >> Crazy Glue >> Duco cement >> silicone (e.g 732 RTV) >> rubber cement >> two sided scotch tape >> paraffin >> dental wax >> postit note >> gelatin >> PVA blobs >> Hot glue >> Nail varnish >> >> Best, >> Tobias >> >> -- >> Tobias Rose, PhD >> Max-Planck-Institute of Neurobiology >> Department of Cellular and Systems Neurobiology >> Am Klopferspitz 18 >> D-82152 Martinsried >> Phone: +49 89 8578 - 3684 >> Fax: +49 89 8578 - 2481 >> [hidden email] >> http://www.neuro.mpg.de/37634/rose >> >> >> > -----Original Message----- >> > From: Confocal Microscopy List >> > [mailto:[hidden email]] On Behalf Of Dan Focht >> > Sent: Tuesday, June 26, 2012 8:26 AM >> > To: [hidden email] >> > Subject: Re: Removable glue for optics >> > >> > ***** >> > To join, leave or search the confocal microscopy listserv, go to: >> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> > ***** >> > >> > Tobias >> > >> > >> > This is old but it works for temporary bonds. >> > Collodian. >> > >> > Dries fast, light to medium hold, dissolves easily and most >> > importantly, >> > cheap! >> > Caution, flammable when in liquid form. >> > You might find it in the OR of a hospital. >> > >> > >> > Dan >> > >> > >> > On Jun 25, 2012, at 11:38 AM, Tobias Rose wrote: >> > >> > > ***** >> > > To join, leave or search the confocal microscopy listserv, go to: >> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> > > ***** >> > > >> > > Dear all, >> > > >> > > I need to glue some glass optics onto anodized aluminum surfaces >> > > (edges of coated dichroic mirror surface and of a full reflective >> > > silver mirror). The problem is that I'd like to be able to remove the >> > > mirrors at some point without completely ruining their reflective >> > > surfaces and leaving too much residue on the glass and aluminum. >> > > The glued parts are not in the optical path so the glue does not have >> > > to be transparent. Also the glue can be quite weak, just enough so >> > > that the mirrors don't fall off. >> > > >> > > Can anyone suggest a nice glue for that? >> > > >> > > Best, >> > > Tobias >> > >> > Dan Focht >> > [hidden email] >> > Bioptechs, Inc. >> > 3560 Beck Rd. >> > Butler, PA 16002 >> > www.bioptechs.com >> > Direct 724-282-7145 >> > Fax 724-282-0745 >> > Toll Free 877 LIVE-CELL - (548-3235) > -- Gregg Jarvis Advanced Products Group Omega Optical Inc. 24 Omega Drive Brattleboro, VT 05301 [hidden email] 1-802-251-7316 |
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Matthew Nicholas-2 |
Re: Removable glue for optics
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** On Tue, Jun 26, 2012 at 11:41 AM, Gregg Jarvis <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Chroma dichroics should be hard oxide or sputtered, should be no > problem for solvents. > Just to reiterate my earlier point regarding dichroic mirrors and hard-drying glue (e.g. cyanoacrylate (Krazy glue) or nail polish) -- this was an issue I encountered when using Chroma mirrors (even on the thicker 1.5mm versions specifically for lasers; I expect this would happen with any mirror less than about 0.5" thick, and maybe even with these). If you are using the dichroic in a pathway for a laser, it will almost certainly mess up your beam profile when using these glues/cements because they literally bends the mirror as they dry. Conversely, a dab of soft-drying glue should be possible to apply in such a way that there is no contact with the optical surface (only the mirror edge -- if it is rather gelatinous/thick to start out with, it will not be drawn onto the optical surface.). This can usually be removed without solvent while leaving negligible residue. The other issue with using solvents, even if the mirror can tolerate them, is that you increase the risk of spreading the glue around unless you are very careful. |
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In reply to this post by Zac Arrac Atelaz
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hello All, We are planning on purchasing a CMOS camera from either Andor (Neo) or Hamamatsu (ORCA Flash 4). We are going to be demo them before deciding. However, looking at the specs, ORCA claims 70% QE vs 55% for Neo. Since both of them have the same chip, is this information accurate. Any help in resolving this question is appreciated. Thanks, -Prabhakar |
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In reply to this post by Matthew Nicholas-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** It seems like you've already got lot of good suggestions, but I'll add my two cents and suggest that you err on the side of making sure that the mirrors/dichroics don't fall off. A new mirror is cheaper than a new eye, and quite possibly also cheaper than the time you might spend on realignment if a mirror does detach itself. In my opinion this rules out double sided tape, and other adhesives of similar strength. When working on a home built interferometric microscope we initially used double sided tape to attach mirrors to their mounts, but switched to cyanoacrylate (superglue) once a couple of them had fallen off, sending the laser beam on an unpredictable course and necessitating a whole day spent on realignment. This was special strong double sided tape which was nominally suitable for optics mounting but it still failed. The really insidious bit was that failure occurred about 6 months down the track, and with very little warning. These were front surface mirrors on an 8-10mm substrate and we applied the glue over the whole mirror back. In this configuration we didn't see any deleterious effects on wavefront quality, but it could certainly be an issue with thinner dichroics which are only secured at the edges. cheers, David ________________________________ From: Matthew Nicholas <[hidden email]> To: [hidden email] Sent: Wednesday, 27 June 2012 5:30 AM Subject: Re: Removable glue for optics ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** On Tue, Jun 26, 2012 at 11:41 AM, Gregg Jarvis <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Chroma dichroics should be hard oxide or sputtered, should be no > problem for solvents. > Just to reiterate my earlier point regarding dichroic mirrors and hard-drying glue (e.g. cyanoacrylate (Krazy glue) or nail polish) -- this was an issue I encountered when using Chroma mirrors (even on the thicker 1.5mm versions specifically for lasers; I expect this would happen with any mirror less than about 0.5" thick, and maybe even with these). If you are using the dichroic in a pathway for a laser, it will almost certainly mess up your beam profile when using these glues/cements because they literally bends the mirror as they dry. Conversely, a dab of soft-drying glue should be possible to apply in such a way that there is no contact with the optical surface (only the mirror edge -- if it is rather gelatinous/thick to start out with, it will not be drawn onto the optical surface.). This can usually be removed without solvent while leaving negligible residue. The other issue with using solvents, even if the mirror can tolerate them, is that you increase the risk of spreading the glue around unless you are very careful. |
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In reply to this post by B. Prabhakar Pandian
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Prabhakar, Do you know for a fact they use the same chip? The Andor sCMOS camera's spec sheet reports 5.5 MP (2,560 x 2,160 pixels) while Hamamatsu reports 2,048 x 2,048 effective pixels for the Flash 4. Could still be the same chip but why would Hamamatsu waste 1.5 MP? Also, the QE curve of the Andor resembles the QE curves of what Hamamatsu calls "Generation I" sCMOS, while the Flash 4 uses a "Gen II" chip with higher QE.... I'd suggest asking the respective reps. Maybe Andor is just a bit behind and will release an sCMOS with 70% QE in the next few months, or they chose a different chip for some other reason... -- Julio Vazquez Fred Hutchinson Cancer Research Center Seattle, WA 98109-1024 http://www.fhcrc.org/ On Jun 26, 2012, at 2:17 PM, B. Prabhakar Pandian wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hello All, > We are planning on purchasing a CMOS camera from either Andor (Neo) or Hamamatsu (ORCA Flash 4). We are going to be demo them > before deciding. However, looking at the specs, ORCA claims 70% QE vs 55% for Neo. Since both of them have the same chip, is this information accurate. > Any help in resolving this question is appreciated. > > Thanks, > > -Prabhakar |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Was just about to say the same thing - as far as I know they use a different chip (PCO and Andor both use the same chip, but Hamamatsu wasn't part of that consortium and decided to go it alone). The Gen I vs Gen II is probably mostly marketing hype ... In addition to QE I'd also pay attention to hot pixels and pixel to pixel gain/noise variations, as these tend to be the limiting features of sCMOS technology at the moment (you might get a mean read noise of ~ 1 e-, but a handful of pixels have much larger read noises). The manufacturers tend to offer soft/firmware solutions to mask these noisy pixels (often replacing the value by interpolation from neighbouring pixels), but whether this is desirable or not will depend on the application (it's one of the major concerns when evaluating sCMOS for PALM/STORM applications). cheers, David ________________________________ From: Julio Vazquez <[hidden email]> To: [hidden email] Sent: Wednesday, 27 June 2012 12:11 PM Subject: Re: CMOS Camera ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Prabhakar, Do you know for a fact they use the same chip? The Andor sCMOS camera's spec sheet reports 5.5 MP (2,560 x 2,160 pixels) while Hamamatsu reports 2,048 x 2,048 effective pixels for the Flash 4. Could still be the same chip but why would Hamamatsu waste 1.5 MP? Also, the QE curve of the Andor resembles the QE curves of what Hamamatsu calls "Generation I" sCMOS, while the Flash 4 uses a "Gen II" chip with higher QE.... I'd suggest asking the respective reps. Maybe Andor is just a bit behind and will release an sCMOS with 70% QE in the next few months, or they chose a different chip for some other reason... -- Julio Vazquez Fred Hutchinson Cancer Research Center Seattle, WA 98109-1024 http://www.fhcrc.org/ On Jun 26, 2012, at 2:17 PM, B. Prabhakar Pandian wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hello All, > We are planning on purchasing a CMOS camera from either Andor (Neo) or Hamamatsu (ORCA Flash 4). We are going to be demo them > before deciding. However, looking at the specs, ORCA claims 70% QE vs 55% for Neo. Since both of them have the same chip, is this information accurate. > Any help in resolving this question is appreciated. > > Thanks, > > -Prabhakar |
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In reply to this post by B. Prabhakar Pandian
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Prabhakar, They are indeed different chips, hence different characteristics. Andor is using the Fairchild chip. There are other sCMOS cameras to consider as well. Raptor offers a sCMOS camera (Osprey) which uses a CMOSIS chip (you can Google these guys to find out more information). This is a 4.2MP (2048x2048) chip with 64% QE at 600nm. It also uses a global shutter, something to consider. It is a small, light camera that offers a very attractive price point. Feel free to contact me for more information. Mark |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** It is generally considered good form to put "Commercial Response" in the header of messages like this. Of course we all value messages from vendors, but it is nice to be able to distinguish them. Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Guy Cox, MA, DPhil(Oxon), Honorary Associate, Australian Centre for Microscopy & Microanalysis, Madsen Building F09, University of Sydney, NSW 2006 Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Mark Donaghy Sent: Wednesday, 27 June 2012 7:03 PM To: [hidden email] Subject: Re: CMOS Camera ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Prabhakar, They are indeed different chips, hence different characteristics. Andor is using the Fairchild chip. There are other sCMOS cameras to consider as well. Raptor offers a sCMOS camera (Osprey) which uses a CMOSIS chip (you can Google these guys to find out more information). This is a 4.2MP (2048x2048) chip with 64% QE at 600nm. It also uses a global shutter, something to consider. It is a small, light camera that offers a very attractive price point. Feel free to contact me for more information. Mark |
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In reply to this post by Mark Cannell
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Tobias, A colleague showed to me a polymer that is made to clean optical surfaces. It works like a glue: you pour it onto the surface, it polymerizes and then you peel it off like silicon with all the dirt and dust. So it should work temporarily like also as an adhesive to stick a mirror to another surface. The advantage is, its made for optics so it will not harm any coatings or delicate surface. See link below Best regards, Thomas ETH Basel No commercial interest http://www.photoniccleaning.com/products/#original solutions -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Mark Cannell Sent: Montag, 25. Juni 2012 17:45 To: [hidden email] Subject: Re: Removable glue for optics ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Three thoughts: PVA blobs which can be peeled after setting or hot glue (stronger -harder to peel). Nail varnish is good too. I've used all 3 but YMMV depending on area of contact etc. HTH Mark On 25/06/2012, at 4:38 PM, Tobias Rose wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear all, > > I need to glue some glass optics onto anodized aluminum surfaces (edges of coated dichroic mirror surface and of a full reflective silver mirror). The problem is that I'd like to be able to remove the mirrors at some point without completely ruining their reflective surfaces and leaving too much residue on the glass and aluminum. > The glued parts are not in the optical path so the glue does not have to be transparent. Also the glue can be quite weak, just enough so that the mirrors don't fall off. > > Can anyone suggest a nice glue for that? > > Best, > Tobias |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Interesting! Especially because I am looking for a better fixation solution in the first place because I made a total mess out of my dichroic and mirror in my second mounting attempt: My first approach was to use set screws to fix the glass. Turns out this is a bad idea (as has been said several times now): The stress introduced to the glass completely distorted any image. My second attempt was to use UV curable optical adhesives (Norland Type OA61 for fixed and NBA107 for reversible mounting). This was an even bigger disaster: Turns out that a mercury arc lamp without excitation filters does _not_ cure the adhesives overnight _under_ the glass (note to self: mirrors reflect light...). So the dichroics gently sled over the glued surfaces and were nicely and uniformly covered in UV adhesive the next day... Acetone (or water) followed by the polymer cleaning as you suggested might save them still (I certainly ordered new ones to be safe). Thanks again for all the suggestions, Tobias > -----Original Message----- > From: Confocal Microscopy List > [mailto:[hidden email]] On Behalf Of Horn > Thomas > Sent: Wednesday, June 27, 2012 11:29 AM > To: [hidden email] > Subject: Re: Removable glue for optics > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi Tobias, > A colleague showed to me a polymer that is made to clean optical surfaces. > It works like a glue: you pour it onto the surface, it polymerizes and then > you peel it off like silicon with all the dirt and dust. So it should work > temporarily like also as an adhesive to stick a mirror to another surface. The > advantage is, its made for optics so it will not harm any coatings or delicate > surface. See link below Best regards, Thomas ETH Basel > > > No commercial interest > > > http://www.photoniccleaning.com/products/#original solutions > > > > > > -----Original Message----- > From: Confocal Microscopy List > [mailto:[hidden email]] On Behalf Of Mark > Cannell > Sent: Montag, 25. Juni 2012 17:45 > To: [hidden email] > Subject: Re: Removable glue for optics > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Three thoughts: > > PVA blobs which can be peeled after setting or hot glue (stronger -harder to > peel). Nail varnish is good too. I've used all 3 but YMMV depending on area > of contact etc. > > HTH > > Mark > > > On 25/06/2012, at 4:38 PM, Tobias Rose wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > Dear all, > > > > I need to glue some glass optics onto anodized aluminum surfaces (edges > of coated dichroic mirror surface and of a full reflective silver mirror). The > problem is that I'd like to be able to remove the mirrors at some point > without completely ruining their reflective surfaces and leaving too much > residue on the glass and aluminum. > > The glued parts are not in the optical path so the glue does not have to be > transparent. Also the glue can be quite weak, just enough so that the > mirrors don't fall off. > > > > Can anyone suggest a nice glue for that? > > > > Best, > > Tobias |
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