Resizing/croping

[Mike Tighe]

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A journal has asked me to re-size an Image to 8.5" X 11".  Can I do this in ImageJ/Fiji ? The original image is 2048 X 2048 and 16MB and they need at least 300 DPI.



Thanks for any help!

Mike

[Julio Vazquez]

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Your image is square, so you probably should keep it square.  8.5 inches at 300 dpi adds up to 2550 pixels. Open your image, go to image > adjust > size and enter 2550 x 2550 (keep aspect ratio, use bilinear or bicubic interpolation). If you want to print it at 8.5 x 11, you will need to crop it to the same aspect ratio, and then upsample it as described above.

A better idea though would be to print your image at a final size of 6.8 x 6.8 inches. This is still 300 dpi, but you are not doing any upsampling (and therefore changing your data)

Julio Vazquez
Fred Hutchinson Cancer Research Center
Seattle, USA


http://www.fhcrc.org

==


On Mar 5, 2013, at 12:49 PM, Mike Tighe wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> A journal has asked me to re-size an Image to 8.5" X 11".  Can I do this in ImageJ/Fiji ? The original image is 2048 X 2048 and 16MB and they need at least 300 DPI.
>
>
>
> Thanks for any help!
>
> Mike

[Johannes Schindelin]

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Hi Mike,

On Tue, 5 Mar 2013, Mike Tighe wrote:

> A journal has asked me to re-size an Image to 8.5" X 11".  Can I do this
> in ImageJ/Fiji ? The original image is 2048 X 2048 and 16MB and they
> need at least 300 DPI.

It is possible: Analyze>Set Scale... lets you choose the unit "in" for
"inch", then just input the width in pixels and the desired width in inch
in the first two input boxes.

Saving as .tif and .jpg should heed those settings, and I could imagine
.png does it, too.

Ciao,
Johannes

[Julio Vazquez]

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I should add that ImageJ doesn't know about DPIs (as far as I know).... The Jounal may be smart enough to know what size your image needs to be printed, but I could be wrong. If you want to be sure that your image prints at the desired size, you may be better off using Photoshop or similar software. In this case, you can assign your desired DPI to the Figure, and therefore specify the printing size at the same time (image size in pixels  divided by DPI  equals print size in inches).

Julio Vazquez
Fred Hutchinson Cancer Research Center
Seattle, USA


http://www.fhcrc.org

==


On Mar 5, 2013, at 12:49 PM, Mike Tighe wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> A journal has asked me to re-size an Image to 8.5" X 11".  Can I do this in ImageJ/Fiji ? The original image is 2048 X 2048 and 16MB and they need at least 300 DPI.
>
>
>
> Thanks for any help!
>
> Mike

[Gitta Hamel]

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/Commercial response/

Hello Mike,

In free ware mode the Huygens Essential and Professional software allows
you to crop, split and convert your images. Some proprietary fileformats
require an active Huygens license but all common fileformats are read in
at all times.

Some of the visualization tools (Twin-slicer and Volume renderer) are
active in free ware mode as well.

Saving your cropped data can be done in for example RGB Tif, 8 and 16
bit Tifs , ICS, OME, DV, HDF5.


With kind wishes,

Gitta Hamel


****************************************
Gitta Hamel
Managing Director Scientific Volume Imaging bv
Developers of the *HUYGENS* software
Laapersveld 63
1213 VB Hilversum
The Netherlands
phone: ++ 31 35 6 42 16 26
Skype: gittahamel
Fax: ++ 31 35 6 83 79 71
*****************************************


^SVI Customer support: mail us your questions [hidden email]
<mailto:[hidden email]>or find answers online in our Huygens
WIKIwww.svi.nl/FrontPage <http://www.svi.nl/FrontPage>
On 03/05/2013 10:24 PM, Julio Vazquez wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> I should add that ImageJ doesn't know about DPIs (as far as I know).... The Jounal may be smart enough to know what size your image needs to be printed, but I could be wrong. If you want to be sure that your image prints at the desired size, you may be better off using Photoshop or similar software. In this case, you can assign your desired DPI to the Figure, and therefore specify the printing size at the same time (image size in pixels  divided by DPI  equals print size in inches).
>
> Julio Vazquez
> Fred Hutchinson Cancer Research Center
> Seattle, USA
>
>
> http://www.fhcrc.org
>
> ==
>
>
> On Mar 5, 2013, at 12:49 PM, Mike Tighe wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> A journal has asked me to re-size an Image to 8.5" X 11".  Can I do this in ImageJ/Fiji ? The original image is 2048 X 2048 and 16MB and they need at least 300 DPI.
>>
>>
>>
>> Thanks for any help!
>>
>> Mike

--
Managing Director

Huygens SVI

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fax:  [phone]

skype: gittahamel

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Visiting address

Laapersveld 63,
1213 VB Hilversum,
The Netherlands

[Jerome Mutterer]

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Hi Mike,
you could give a try to FigureJ, an ImageJ plugin that was designed to
achieve this kind of figure operation (and more).

In FigureJ, create a new figure with dimensions of 8.5 by 11 inches at 300
dpi (new figure dialog)
Then choose 'open image', select your original data file.
Visually select the region to be scaled and press 'OK'. Done.

See videos at http://www.figurej.org/ for more detais.

Jerome

On 5 March 2013 21:49, Mike Tighe <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> A journal has asked me to re-size an Image to 8.5" X 11".  Can I do this
> in ImageJ/Fiji ? The original image is 2048 X 2048 and 16MB and they need
> at least 300 DPI.
>
>
>
> Thanks for any help!
>
> Mike
>