Round coverslips for immunostaining
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Dries Vercauteren |
Round coverslips for immunostaining
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Search the CONFOCAL archive at
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Dear all,
I'd need round coverslips for immunostaining cells in wells of 24-well plates. Therefore, I was looking for appropriate coverslips at Menzel. But as you can see http://www.menzel.de/Cover_Slips.675.0.html?L=1, Menzel has only coverslips of class 1, instead I'd need a 0.17mm thickness (which is best approximated in class 1.5). Thus, my questions are;
Take care, Dries. -- Dries Vercauteren, PhD student Master of Bioscience Engineering: Cell and Gene Biotechnology Ghent Research Group on Nanomedicines www.ugent.be/fw/en/research/biofys Faculty of pharmaceutical sciences, Ghent University Harelbekestraat 72, 9000 Ghent Belgium Phone: +329/264 80 49 Mobile: +32485/30 69 80 E-mail: [hidden email] [hidden email] |
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Florian Eich |
Re: Round coverslips for immunostaining
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi Dries, during my PhD I had good experience with the 24-well plates with glass bottom from http://www.glass-bottom-dishes.com/ (no comercial interest). If you want to have individual coverslips try http://www.knittel-glaeser.de/ (again no com. interest). They will produce custom diameters if you want. Regarding the diameter I found it better to use 12mm, giving you a bit of space to lift the coverslip out of the well. Best Florian |
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Sylvie Le Guyader-2 |
Re: Round coverslips for immunostaining
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In reply to this post by Dries Vercauteren
Search the CONFOCAL archive at
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Hi Dries We routinely use 12mm #1.5 coverslips for
24-well plates. You can find them at Warner Instruments. http://www.warneronline.com/product_info.cfm?id=11&alpha=a&name=Coverslip%2FCoverglass Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader Dept of Biosciences and Nutrition Karolinska Institutet Novum 14157 +46 (0)8 608 9269 From:
Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Dries Vercauteren Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear all,
Many thanks in advance for the advice! |
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Beat Ludin |
Re: Round coverslips for immunostaining
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In reply to this post by Dries Vercauteren
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi Dries I would recommend the thickness-selected 0.17mm (=type 1014) coverslips from "assistent" (http://www.hecht-assistent.de). Use 12mm coverslips for 24-well plates. Larger coverslips are difficult to get out of the wells. No commercial interest. Beat At 10:53 22-08-2008, you wrote: >Search the CONFOCAL archive at >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >Dear all, > >I'd need round coverslips for immunostaining cells in wells of >24-well plates. Therefore, I was looking for appropriate coverslips >at Menzel. But as you can see ><http://www.menzel.de/Cover_Slips.675.0.html?L=1>http://www.menzel.de/Cover_Slips.675.0.html?L=1, >Menzel has only coverslips of class 1, instead I'd need a 0.17mm >thickness (which is best approximated in class 1.5). >Thus, my questions are; > * what is the ideal diameter of the round coverslips to place > them in 24-wells? 12, 13 or 14 mm? > * Is there another possibility (another company) to buy high > quality coverslips of #1.5? >Many thanks in advance for the advice! > >Take care, > >Dries. >-- >Dries Vercauteren, PhD student >Master of Bioscience Engineering: Cell and Gene Biotechnology > >Ghent Research Group on Nanomedicines ><http://www.ugent.be/fw/en/research/biofys>www.ugent.be/fw/en/research/biofys >Faculty of pharmaceutical sciences, Ghent University >Harelbekestraat 72, 9000 Ghent >Belgium > >Phone: +329/264 80 49 >Mobile: +32485/30 69 80 >E-mail: [hidden email] ><mailto:[hidden email]>[hidden email] |
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RICHARD BURRY |
Re: Round coverslips for immunostaining
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In reply to this post by Dries Vercauteren
Search the CONFOCAL archive at
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Dries Yes, 12 mm is the best, because anything bigger is impossible to get out of the well. 12 mm gives a good amount of area for growing cells, anything small well limit the number of cells. Please experiment with ways to remover the cover slip and still know which side has the cells! Until mastered this was our only problem. Dick R. W. Burry, Campus Microscopy and Imaging Facility, Director The Ohio State University 277 Biomedical Research Tower 460 West Twelfth Avenue Columbus, Ohio 43210 ----- Original Message ----- From: Dries Vercauteren <[hidden email]> Date: Friday, August 22, 2008 5:03 am Subject: Round coverslips for immunostaining To: [hidden email] > Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear all,
> I'd need round coverslips for immunostaining cells in wells of 24-well plates. Therefore, I was looking for appropriate coverslips at Menzel. But as you can see > http://www.menzel.de/Cover_Slips.675.0.html?L=1, Menzel has only coverslips of class 1, instead I'd need a 0.17mm thickness (which is best approximated in class 1.5). > Thus, my questions are;
> Take care, > Dries. > -- > Dries Vercauteren, PhD student > Master of Bioscience Engineering: Cell and Gene Biotechnology > Ghent Research Group on Nanomedicines > www.ugent.be/fw/en/research/biofys > Faculty of pharmaceutical sciences, Ghent University > Harelbekestraat 72, 9000 Ghent > Belgium > Phone: +329/264 80 49 > Mobile: +32485/30 69 80 > E-mail: [hidden email] <A href="javascript:main.compose('new','t=driesvercauteren@gmail.com')">> driesvercauteren@... > Spam > Not spam > Forget previous vote |
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Sam's Mail |
Re: Round coverslips for immunostaining. .
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In reply to this post by Beat Ludin
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Does anyone know who distributes this Hecht-assistant 0.17mm thick glass here in the US? -- Samuel A. Connell Director of Light Microscopy Cell & Tissue Imaging Center St. Jude Children's Research Hospital 262 Danny Thomas Place Memphis, TN 38105-3678 Office (901) 495-2536 Cell (901) 603-3162 [hidden email] -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Beat Ludin Sent: Friday, August 22, 2008 6:04 AM To: [hidden email] Subject: Re: Round coverslips for immunostaining. . Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi Dries I would recommend the thickness-selected 0.17mm (=type 1014) coverslips from "assistent" (http://www.hecht-assistent.de). Use 12mm coverslips for 24-well plates. Larger coverslips are difficult to get out of the wells. No commercial interest. Beat At 10:53 22-08-2008, you wrote: >Search the CONFOCAL archive at >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >Dear all, > >I'd need round coverslips for immunostaining cells in wells of >24-well plates. Therefore, I was looking for appropriate coverslips >at Menzel. But as you can see ><http://www.menzel.de/Cover_Slips.675.0.html?L=1>http://www.menzel.de/Cover_Slips.675.0.html?L=1, >Menzel has only coverslips of class 1, instead I'd need a 0.17mm >thickness (which is best approximated in class 1.5). >Thus, my questions are; > * what is the ideal diameter of the round coverslips to place > them in 24-wells? 12, 13 or 14 mm? > * Is there another possibility (another company) to buy high > quality coverslips of #1.5? >Many thanks in advance for the advice! > >Take care, > >Dries. >-- >Dries Vercauteren, PhD student >Master of Bioscience Engineering: Cell and Gene Biotechnology > >Ghent Research Group on Nanomedicines ><http://www.ugent.be/fw/en/research/biofys>www.ugent.be/fw/en/research/biofys >Faculty of pharmaceutical sciences, Ghent University >Harelbekestraat 72, 9000 Ghent >Belgium > >Phone: +329/264 80 49 >Mobile: +32485/30 69 80 >E-mail: [hidden email] ><mailto:[hidden email]>[hidden email] |
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Stephen Cody |
Re: Round coverslips for immunostaining
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In reply to this post by Beat Ludin
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal G'day dries, As an alternative to the coverslips that Beat recommends, you should also be able to order special confocal thickness coverslips from Menzel, by special order in any size you wish. The thicness recommended for confocal, especially if you are going to use a water immersion lens is "Grade: 0.170 +/- 0.01mm". We order larger round coverslips of this thicness through an Australian agency (Lomb), but they custom order them from Menzel. This has been discussed previously on the list, se subject: "Coverslip - Good News" Cheers Stephen H. Cody Microscopy Manager Central Resource for Advanced Microscopy Ludwig Institute for Cancer Research PO Box 2008 Royal Melbourne Hospital Parkville, Victoria, 3050 Australia Tel: 61 3 9341 3155 Fax: 61 3 9341 3104 email: [hidden email] www.ludwig.edu.au/labs/confocal.html www.ludwig.edu.au/confocal Tip: Learn how to receive reminders about you microscope booking: http://www.ludwig.edu.au/confocal/Local/Booking_Hint.htm Type your signature here ________________________________ From: Confocal Microscopy List on behalf of Beat Ludin Sent: Fri 22/08/2008 9:04 PM To: [hidden email] Subject: Re: Round coverslips for immunostaining Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi Dries I would recommend the thickness-selected 0.17mm (=type 1014) coverslips from "assistent" (http://www.hecht-assistent.de <http://www.hecht-assistent.de/> ). Use 12mm coverslips for 24-well plates. Larger coverslips are difficult to get out of the wells. No commercial interest. Beat At 10:53 22-08-2008, you wrote: >Search the CONFOCAL archive at >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >Dear all, > >I'd need round coverslips for immunostaining cells in wells of >24-well plates. Therefore, I was looking for appropriate coverslips >at Menzel. But as you can see ><http://www.menzel.de/Cover_Slips.675.0.html?L=1>http://www.menzel.de/Cover_Slips.675.0.html?L=1, >Menzel has only coverslips of class 1, instead I'd need a 0.17mm >thickness (which is best approximated in class 1.5). >Thus, my questions are; > * what is the ideal diameter of the round coverslips to place > them in 24-wells? 12, 13 or 14 mm? > * Is there another possibility (another company) to buy high > quality coverslips of #1.5? >Many thanks in advance for the advice! > >Take care, > >Dries. >-- >Dries Vercauteren, PhD student >Master of Bioscience Engineering: Cell and Gene Biotechnology > >Ghent Research Group on Nanomedicines ><http://www.ugent.be/fw/en/research/biofys>www.ugent.be/fw/en/research/biofys >Faculty of pharmaceutical sciences, Ghent University >Harelbekestraat 72, 9000 Ghent >Belgium > >Phone: +329/264 80 49 >Mobile: +32485/30 69 80 >E-mail: [hidden email] ><mailto:[hidden email]>[hidden email] |
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Sylvie Le Guyader-2 |
Re: Round coverslips for immunostaining
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In reply to this post by RICHARD BURRY
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hi again Dries To remove coverslips from wells I use a
long needle (of the type to do injections) bent to get just the tip at a 90º
angle. I dip the needle tip and a pair of flat tweezers into Ethanol to
sterilize them then I scratch the bottom of the well with the needle tip until
it catches and lifts the coverslip a bit. I then carefully grab the coverslip
with the flat tweezers, making sure I keep the side that has the cells towards
the top. You will break a couple of coverslips first then you’ll become
an expert and will be able to do it very quickly and reliably. Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader Dept of Biosciences and Nutrition Karolinska Institutet Novum 14157 +46 (0)8 608 9269 From:
Confocal Microscopy List [mailto:[hidden email]] On Behalf Of RICHARD BURRY Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear all,
Many thanks in advance for
the advice! |
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Dries Vercauteren |
Re: Round coverslips for immunostaining
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Dear all,
many thanks to anyone of you for all these helpful comments! Take care! Dries.
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Jose Paulo Sequeira Farinha |
Open Confocal/Multiphoton/FCS research position in Lisbon, Portugal
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Main research field: Chemistry Sub research field: Fluorescence Spectroscopy and Imaging Job description: The researcher will develop an independent project in Fluorescence Spectroscopy and Imaging, specifically in Confocal/Biphotonic Fluorescence Microscopy and Fluorescence Correlation Spectroscopy. Centro de Química-Física Molecular (CQFM, http://dequim.ist.utl.pt/CQFM) is part of IN - Institute of Nanoscience and Nanotechnology, and a research center of Instituto Superior Técnico, one of the oldest and largest Schools of Science and Engineering in Portugal Duties of successful candidate include responsibility for a research project to be developed within the unit. There will be no teaching duties. The working language is English. The successful candidates will receive a salary in accordance with the university regulations for a senior researcher. The contract offered will have duration of up to 5 years, renewed yearly based on mutual agreement. The annual gross income, before taxes, will be approximately 43,000 Euros. Applicants Applicants should have obtained a Ph. D. in one of the following areas: Chemistry, Physics, Biochemistry, Chemical Engineering, Biological Engineering and Materials. Candidates should have a high quality research record and research experience. The successful candidates are expected to do research in the areas listed above. For more information, consult http://www.eracareers.pt How to Apply By email to: [hidden email], with the following information: - Identification of the candidate - Curriculum Vitae/resume - Letters of Reference - Statement of purpose for the period of the contract (up to 3 pages). Good weather is bonus... |
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