*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopyPost images on
http://www.imgur.com and include the link in your posting.
*****
Hi Ben,
Which SIM system are you considering? We have an OMX in a room that -
despite their assurances to me when designing it - sadly does fluctuate
by more than 1 degree. I would say that our room temperature is
generally somewhere between 22 and 25 degrees (or so the software says -
has to be a lie, we are bundled up in coats in there it's so cold!), but
it's the rate of change that seems to be critical. We find that if the
temperature changes by 1 degree, you have to change the oil to the next
refractive index one e.g. from 1.512 to 1.514. So if it takes several
hours to change by that much, it's not really a problem, you'll probably
have finished your session by then or at least moved onto another sample
and can use a different oil. But if it fluctuates by a degree in less
than an hour you may drive yourself crazy constantly taking your sample
off to change the oil. Of course this will not be at all relevant to
the Zeiss or Nikon systems, which don't come with a big range of r.i.
oils - that is why I asked which system you're talking about.
Hope that helps...
All the best,
Alison
On 2/4/2015 2:17 PM, Benjamin Abrams wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
http://www.imgur.com and include the link in your posting.
> *****
>
> Hi All,
>
> We are looking in to getting a SIM system and see that the room requirements
> are really strict, +/- 0.5 deg C. This would require significant room
> modification (possibly several $100K worth). +/- 1.0 deg C is more doable.
> What is the consensus out there? How necessary is +/- 0.5? Can anyone
> comment who has experience running their system in a room outside of spec
> (even temporarily)?
>
> Thanks,
> Ben
--
Alison J. North, Ph.D.,
Senior Director of the Bio-Imaging Resource Center and
Research Associate Professor,
The Rockefeller University,
1230 York Avenue,
New York,
NY 10065.
Tel: office ++ 212 327 7488
Tel: lab ++ 212 327 7486
Fax: ++ 212 327 7489
Locked
