SP2 Shutter

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Christina Polumbo Christina Polumbo
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SP2 Shutter

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Fellow Microphotographers,

The shutter for the widefield fluorescent light on our Leica SP2 confocal is broken. It uses a standard HBO mercury bulb, so no apertures or Xcite shuttering is available on the system. Since the microscope is an older model, were having a tough time getting a replacement from the vendor, that works. They sent us one, which was defective (last inspected in 2007) and theyre having a tough time finding another one for us.

We discussed having a simple pull and push shutter system built, since its easy enough to do I suppose and perhaps even purchasing an Xcite with the idea of applying it to a different microscope when the SP2 eventually died (which might be sooner than later unfortunately). Before going through all of that, I thought that it might be worth a shot to just try and see if anyone has recently decommissioned an SP2 confocal, or perhaps purchased an Xcite for their system. If anyone has a spare shutter from the original set up of their microscope laying around and wouldnt mind donating it to our facility, we would very much appreciate it. Please just reply to this message and I would be happy to send along our information for shipping. We would obviously pay for the shipping and any out of pocket costs.

Thank you,
Christina Polumbo

Analytical Imaging Facility
Albert Einstein College of Medicine
Gregg Jarvis Gregg Jarvis
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Re: SP2 Shutter

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send a photo to me
[hidden email]

On Tue, Mar 19, 2013 at 12:45 PM, Christina Polumbo <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Fellow Microphotographers,
>
> The shutter for the widefield fluorescent light on our Leica SP2 confocal
> is broken. It uses a standard HBO mercury bulb, so no apertures or Xcite
> shuttering is available on the system. Since the microscope is an older
> model, were having a tough time getting a replacement from the vendor, that
> works. They sent us one, which was defective (last inspected in 2007) and
> theyre having a tough time finding another one for us.
>
> We discussed having a simple pull and push shutter system built, since its
> easy enough to do I suppose and perhaps even purchasing an Xcite with the
> idea of applying it to a different microscope when the SP2 eventually died
> (which might be sooner than later unfortunately). Before going through all
> of that, I thought that it might be worth a shot to just try and see if
> anyone has recently decommissioned an SP2 confocal, or perhaps purchased an
> Xcite for their system. If anyone has a spare shutter from the original set
> up of their microscope laying around and wouldnt mind donating it to our
> facility, we would very much appreciate it. Please just reply to this
> message and I would be happy to send along our information for shipping. We
> would obviously pay for the shipping and any out of pocket costs.
>
> Thank you,
> Christina Polumbo
>
> Analytical Imaging Facility
> Albert Einstein College of Medicine
>



--
Gregg Jarvis
Senior Spectroscopist
Advanced Products Group
Omega Optical Inc.

Delta Campus, 21 Omega Drive
Brattleboro, VT 05301, USA
Tel: +1 (802) 251-7316
[hidden email]

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Jacqueline Ross Jacqueline Ross
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Re: SP2 Shutter

In reply to this post by Christina Polumbo
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Hi Christina,

Which microscope model do you have? We have the DMRXA2 on our SP2 and it has a manual shutter in addition to the motorised one.

Kind regards,

Jacqui

Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit 
School of Medical Sciences 
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland 1142, NEW ZEALAND

Tel: 64 9 923 7438
Fax: 64 9 373 7484

http://www.fmhs.auckland.ac.nz/sms/biru/


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Christina Polumbo
Sent: Wednesday, 20 March 2013 5:46 a.m.
To: [hidden email]
Subject: SP2 Shutter

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Fellow Microphotographers,

The shutter for the widefield fluorescent light on our Leica SP2 confocal is broken. It uses a standard HBO mercury bulb, so no apertures or Xcite shuttering is available on the system. Since the microscope is an older model, were having a tough time getting a replacement from the vendor, that works. They sent us one, which was defective (last inspected in 2007) and theyre having a tough time finding another one for us.

We discussed having a simple pull and push shutter system built, since its easy enough to do I suppose and perhaps even purchasing an Xcite with the idea of applying it to a different microscope when the SP2 eventually died (which might be sooner than later unfortunately). Before going through all of that, I thought that it might be worth a shot to just try and see if anyone has recently decommissioned an SP2 confocal, or perhaps purchased an Xcite for their system. If anyone has a spare shutter from the original set up of their microscope laying around and wouldnt mind donating it to our facility, we would very much appreciate it. Please just reply to this message and I would be happy to send along our information for shipping. We would obviously pay for the shipping and any out of pocket costs.

Thank you,
Christina Polumbo

Analytical Imaging Facility
Albert Einstein College of Medicine
Rosemary.White Rosemary.White
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Re: SP2 Shutter

In reply to this post by Christina Polumbo
*****
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*****

As others have said, which model of SP2/microscope stand do you have? Our
SP2 (installed 2000, still going) has a manual shutter for widefield
fluorescence. The stand we have, DMR, is more manual than most, since I
like it that way...

Maybe you could get the engineering drawings for your shutter and get a
replacement made by one of these 3D printing companies that seem to be
sprouting like weeds. It would probably last as long as your SP2, since
I'm told Leica will guarantee spare parts only until 2015 (at least for
our model).

good luck,
Rosemary White

Dr Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

T 61 2 6246 5475
F 61 2 6246 5334
E [hidden email]


On 20/03/13 3:45 AM, "Christina Polumbo"
<[hidden email]> wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Fellow Microphotographers,
>
>The shutter for the widefield fluorescent light on our Leica SP2 confocal
>is broken. It uses a standard HBO mercury bulb, so no apertures or Xcite
>shuttering is available on the system. Since the microscope is an older
>model, were having a tough time getting a replacement from the vendor,
>that works. They sent us one, which was defective (last inspected in
>2007) and theyre having a tough time finding another one for us.
>
>We discussed having a simple pull and push shutter system built, since
>its easy enough to do I suppose and perhaps even purchasing an Xcite with
>the idea of applying it to a different microscope when the SP2 eventually
>died (which might be sooner than later unfortunately). Before going
>through all of that, I thought that it might be worth a shot to just try
>and see if anyone has recently decommissioned an SP2 confocal, or perhaps
>purchased an Xcite for their system. If anyone has a spare shutter from
>the original set up of their microscope laying around and wouldnt mind
>donating it to our facility, we would very much appreciate it. Please
>just reply to this message and I would be happy to send along our
>information for shipping. We would obviously pay for the shipping and any
>out of pocket costs.
>
>Thank you,
>Christina Polumbo
>
>Analytical Imaging Facility
>Albert Einstein College of Medicine
Csúcs  Gábor-2 Csúcs Gábor-2
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Re: SP2 Shutter

*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

An other (though somewhat more expensive) solution is to purchase an
Uniblitz  (or similar) shutter and insert it between the microscope and
the lamp.

Greetings    Gabor



On 3/19/13 11:55 PM, "[hidden email]" <[hidden email]>
wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>As others have said, which model of SP2/microscope stand do you have? Our
>SP2 (installed 2000, still going) has a manual shutter for widefield
>fluorescence. The stand we have, DMR, is more manual than most, since I
>like it that way...
>
>Maybe you could get the engineering drawings for your shutter and get a
>replacement made by one of these 3D printing companies that seem to be
>sprouting like weeds. It would probably last as long as your SP2, since
>I'm told Leica will guarantee spare parts only until 2015 (at least for
>our model).
>
>good luck,
>Rosemary White
>
>Dr Rosemary White
>CSIRO Plant Industry
>GPO Box 1600
>Canberra, ACT 2601
>Australia
>
>T 61 2 6246 5475
>F 61 2 6246 5334
>E [hidden email]
>
>
>On 20/03/13 3:45 AM, "Christina Polumbo"
><[hidden email]> wrote:
>
>>*****
>>To join, leave or search the confocal microscopy listserv, go to:
>>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>*****
>>
>>Fellow Microphotographers,
>>
>>The shutter for the widefield fluorescent light on our Leica SP2 confocal
>>is broken. It uses a standard HBO mercury bulb, so no apertures or Xcite
>>shuttering is available on the system. Since the microscope is an older
>>model, were having a tough time getting a replacement from the vendor,
>>that works. They sent us one, which was defective (last inspected in
>>2007) and theyre having a tough time finding another one for us.
>>
>>We discussed having a simple pull and push shutter system built, since
>>its easy enough to do I suppose and perhaps even purchasing an Xcite with
>>the idea of applying it to a different microscope when the SP2 eventually
>>died (which might be sooner than later unfortunately). Before going
>>through all of that, I thought that it might be worth a shot to just try
>>and see if anyone has recently decommissioned an SP2 confocal, or perhaps
>>purchased an Xcite for their system. If anyone has a spare shutter from
>>the original set up of their microscope laying around and wouldnt mind
>>donating it to our facility, we would very much appreciate it. Please
>>just reply to this message and I would be happy to send along our
>>information for shipping. We would obviously pay for the shipping and any
>>out of pocket costs.
>>
>>Thank you,
>>Christina Polumbo
>>
>>Analytical Imaging Facility
>>Albert Einstein College of Medicine
Johannes Helm Johannes Helm
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Re: SP2 Shutter

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Gabor has provided a good answer. "Uniblitz" means Vincent Associates,
Rochester, NY, www.uniblitz.com. You most probably would need a shutter
with 35mm or 25mm opening.

Best,

Johannes

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> An other (though somewhat more expensive) solution is to purchase an
> Uniblitz  (or similar) shutter and insert it between the microscope and
> the lamp.
>
> Greetings    Gabor
>
>
>
> On 3/19/13 11:55 PM, "[hidden email]" <[hidden email]>
> wrote:
>
>>*****
>>To join, leave or search the confocal microscopy listserv, go to:
>>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>*****
>>
>>As others have said, which model of SP2/microscope stand do you have? Our
>>SP2 (installed 2000, still going) has a manual shutter for widefield
>>fluorescence. The stand we have, DMR, is more manual than most, since I
>>like it that way...
>>
>>Maybe you could get the engineering drawings for your shutter and get a
>>replacement made by one of these 3D printing companies that seem to be
>>sprouting like weeds. It would probably last as long as your SP2, since
>>I'm told Leica will guarantee spare parts only until 2015 (at least for
>>our model).
>>
>>good luck,
>>Rosemary White
>>
>>Dr Rosemary White
>>CSIRO Plant Industry
>>GPO Box 1600
>>Canberra, ACT 2601
>>Australia
>>
>>T 61 2 6246 5475
>>F 61 2 6246 5334
>>E [hidden email]
>>
>>
>>On 20/03/13 3:45 AM, "Christina Polumbo"
>><[hidden email]> wrote:
>>
>>>*****
>>>To join, leave or search the confocal microscopy listserv, go to:
>>>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>>*****
>>>
>>>Fellow Microphotographers,
>>>
>>>The shutter for the widefield fluorescent light on our Leica SP2
>>> confocal
>>>is broken. It uses a standard HBO mercury bulb, so no apertures or Xcite
>>>shuttering is available on the system. Since the microscope is an older
>>>model, were having a tough time getting a replacement from the vendor,
>>>that works. They sent us one, which was defective (last inspected in
>>>2007) and theyre having a tough time finding another one for us.
>>>
>>>We discussed having a simple pull and push shutter system built, since
>>>its easy enough to do I suppose and perhaps even purchasing an Xcite
>>> with
>>>the idea of applying it to a different microscope when the SP2
>>> eventually
>>>died (which might be sooner than later unfortunately). Before going
>>>through all of that, I thought that it might be worth a shot to just try
>>>and see if anyone has recently decommissioned an SP2 confocal, or
>>> perhaps
>>>purchased an Xcite for their system. If anyone has a spare shutter from
>>>the original set up of their microscope laying around and wouldnt mind
>>>donating it to our facility, we would very much appreciate it. Please
>>>just reply to this message and I would be happy to send along our
>>>information for shipping. We would obviously pay for the shipping and
>>> any
>>>out of pocket costs.
>>>
>>>Thank you,
>>>Christina Polumbo
>>>
>>>Analytical Imaging Facility
>>>Albert Einstein College of Medicine
>


--
P. Johannes Helm

Voice: (+47) 228 51159 (office)
Fax: (+47) 228 51499 (office)
Christina Polumbo Christina Polumbo
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Re: SP2 Shutter

*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Sorry for the delay, folks. We have a DMRE2 stand with no manual shutter. The shutter is located in the back of the body of the scope and an electric pulse triggers it to open and close from the shutter button on the front of the microscope. Thats the part that broke. You pus the button and nothing happens. The filter wheel is also motorized.

The suggestions from everyone are pefectly in-line with what we were also thinking, we do have Uniblitz shutters in the facility but I believe they are all in use at the moment. I will look around for a spare and the controller. I figured that since the microscope was so old, that maybe others had replaced their widefield set up with something else, like an Xcite or something, leaving a old spare shutter lying on a dusty shelf somewhere. Seemed like the most straight forward solution, but we will certainly look at other options!

Thanks for all of the great suggestions!

-Christina

Analytical Imaging Facility
Albert Einstein College of Medicine
________________________________________
From: Confocal Microscopy List [[hidden email]] on behalf of Johannes Helm [[hidden email]]
Sent: Wednesday, March 20, 2013 8:34 AM
To: [hidden email]
Subject: Re: SP2 Shutter

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Gabor has provided a good answer. "Uniblitz" means Vincent Associates,
Rochester, NY, www.uniblitz.com. You most probably would need a shutter
with 35mm or 25mm opening.

Best,

Johannes

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> An other (though somewhat more expensive) solution is to purchase an
> Uniblitz  (or similar) shutter and insert it between the microscope and
> the lamp.
>
> Greetings    Gabor
>
>
>
> On 3/19/13 11:55 PM, "[hidden email]" <[hidden email]>
> wrote:
>
>>*****
>>To join, leave or search the confocal microscopy listserv, go to:
>>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>*****
>>
>>As others have said, which model of SP2/microscope stand do you have? Our
>>SP2 (installed 2000, still going) has a manual shutter for widefield
>>fluorescence. The stand we have, DMR, is more manual than most, since I
>>like it that way...
>>
>>Maybe you could get the engineering drawings for your shutter and get a
>>replacement made by one of these 3D printing companies that seem to be
>>sprouting like weeds. It would probably last as long as your SP2, since
>>I'm told Leica will guarantee spare parts only until 2015 (at least for
>>our model).
>>
>>good luck,
>>Rosemary White
>>
>>Dr Rosemary White
>>CSIRO Plant Industry
>>GPO Box 1600
>>Canberra, ACT 2601
>>Australia
>>
>>T 61 2 6246 5475
>>F 61 2 6246 5334
>>E [hidden email]
>>
>>
>>On 20/03/13 3:45 AM, "Christina Polumbo"
>><[hidden email]> wrote:
>>
>>>*****
>>>To join, leave or search the confocal microscopy listserv, go to:
>>>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>>*****
>>>
>>>Fellow Microphotographers,
>>>
>>>The shutter for the widefield fluorescent light on our Leica SP2
>>> confocal
>>>is broken. It uses a standard HBO mercury bulb, so no apertures or Xcite
>>>shuttering is available on the system. Since the microscope is an older
>>>model, were having a tough time getting a replacement from the vendor,
>>>that works. They sent us one, which was defective (last inspected in
>>>2007) and theyre having a tough time finding another one for us.
>>>
>>>We discussed having a simple pull and push shutter system built, since
>>>its easy enough to do I suppose and perhaps even purchasing an Xcite
>>> with
>>>the idea of applying it to a different microscope when the SP2
>>> eventually
>>>died (which might be sooner than later unfortunately). Before going
>>>through all of that, I thought that it might be worth a shot to just try
>>>and see if anyone has recently decommissioned an SP2 confocal, or
>>> perhaps
>>>purchased an Xcite for their system. If anyone has a spare shutter from
>>>the original set up of their microscope laying around and wouldnt mind
>>>donating it to our facility, we would very much appreciate it. Please
>>>just reply to this message and I would be happy to send along our
>>>information for shipping. We would obviously pay for the shipping and
>>> any
>>>out of pocket costs.
>>>
>>>Thank you,
>>>Christina Polumbo
>>>
>>>Analytical Imaging Facility
>>>Albert Einstein College of Medicine
>


--
P. Johannes Helm

Voice:  (+47) 228 51159 (office)
Fax:    (+47) 228 51499 (office)

Johannes Helm Johannes Helm
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Re: SP2 Shutter

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Christina, you'll have to make sure you have the appropriate shutter and
the appropriate connector. Vincent will otherwise sell you an adapter for
that specific Leica dove tail connector.

ALSO:
There are many different types of shutters, which only differ in small but
essential details! The shutter you will need for an arc lamp,
irrespectively of whether it is Hg or Xe, needs to have the shutter
elements coated AR and you need the high temperature option! Make sure the
shutters you have and want to use fulfill these demands.
Also, on the rear side of the shutter controllers there are on some models
dip switches, which have to be set in order to send the correct electrical
pulse  energies to the shutters. 25mm and 35mm shutters need other
energies than the laser shutters.

Best wishes,
Johannes

> Sorry for the delay, folks. We have a DMRE2 stand with no manual shutter.
> The shutter is located in the back of the body of the scope and an
> electric pulse triggers it to open and close from the shutter button on
> the front of the microscope. Thats the part that broke. You pus the button
> and nothing happens. The filter wheel is also motorized.
>
> The suggestions from everyone are pefectly in-line with what we were also
> thinking, we do have Uniblitz shutters in the facility but I believe they
> are all in use at the moment. I will look around for a spare and the
> controller. I figured that since the microscope was so old, that maybe
> others had replaced their widefield set up with something else, like an
> Xcite or something, leaving a old spare shutter lying on a dusty shelf
> somewhere. Seemed like the most straight forward solution, but we will
> certainly look at other options!
>
> Thanks for all of the great suggestions!
>
> -Christina
>
> Analytical Imaging Facility
> Albert Einstein College of Medicine
> ________________________________________
> From: Confocal Microscopy List [[hidden email]] on
> behalf of Johannes Helm [[hidden email]]
> Sent: Wednesday, March 20, 2013 8:34 AM
> To: [hidden email]
> Subject: Re: SP2 Shutter
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Gabor has provided a good answer. "Uniblitz" means Vincent Associates,
> Rochester, NY, www.uniblitz.com. You most probably would need a shutter
> with 35mm or 25mm opening.
>
> Best,
>
> Johannes
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> An other (though somewhat more expensive) solution is to purchase an
>> Uniblitz  (or similar) shutter and insert it between the microscope and
>> the lamp.
>>
>> Greetings    Gabor
>>
>>
>>
>> On 3/19/13 11:55 PM, "[hidden email]" <[hidden email]>
>> wrote:
>>
>>>*****
>>>To join, leave or search the confocal microscopy listserv, go to:
>>>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>>*****
>>>
>>>As others have said, which model of SP2/microscope stand do you have?
>>> Our
>>>SP2 (installed 2000, still going) has a manual shutter for widefield
>>>fluorescence. The stand we have, DMR, is more manual than most, since I
>>>like it that way...
>>>
>>>Maybe you could get the engineering drawings for your shutter and get a
>>>replacement made by one of these 3D printing companies that seem to be
>>>sprouting like weeds. It would probably last as long as your SP2, since
>>>I'm told Leica will guarantee spare parts only until 2015 (at least for
>>>our model).
>>>
>>>good luck,
>>>Rosemary White
>>>
>>>Dr Rosemary White
>>>CSIRO Plant Industry
>>>GPO Box 1600
>>>Canberra, ACT 2601
>>>Australia
>>>
>>>T 61 2 6246 5475
>>>F 61 2 6246 5334
>>>E [hidden email]
>>>
>>>
>>>On 20/03/13 3:45 AM, "Christina Polumbo"
>>><[hidden email]> wrote:
>>>
>>>>*****
>>>>To join, leave or search the confocal microscopy listserv, go to:
>>>>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>>>*****
>>>>
>>>>Fellow Microphotographers,
>>>>
>>>>The shutter for the widefield fluorescent light on our Leica SP2
>>>> confocal
>>>>is broken. It uses a standard HBO mercury bulb, so no apertures or
>>>> Xcite
>>>>shuttering is available on the system. Since the microscope is an older
>>>>model, were having a tough time getting a replacement from the vendor,
>>>>that works. They sent us one, which was defective (last inspected in
>>>>2007) and theyre having a tough time finding another one for us.
>>>>
>>>>We discussed having a simple pull and push shutter system built, since
>>>>its easy enough to do I suppose and perhaps even purchasing an Xcite
>>>> with
>>>>the idea of applying it to a different microscope when the SP2
>>>> eventually
>>>>died (which might be sooner than later unfortunately). Before going
>>>>through all of that, I thought that it might be worth a shot to just
>>>> try
>>>>and see if anyone has recently decommissioned an SP2 confocal, or
>>>> perhaps
>>>>purchased an Xcite for their system. If anyone has a spare shutter from
>>>>the original set up of their microscope laying around and wouldnt mind
>>>>donating it to our facility, we would very much appreciate it. Please
>>>>just reply to this message and I would be happy to send along our
>>>>information for shipping. We would obviously pay for the shipping and
>>>> any
>>>>out of pocket costs.
>>>>
>>>>Thank you,
>>>>Christina Polumbo
>>>>
>>>>Analytical Imaging Facility
>>>>Albert Einstein College of Medicine
>>
>
>

--
P. Johannes Helm, M.Sc. PhD
Seniorengineer
CMBN
University of Oslo
Institute of Basic Medical Science
Department of Anatomy
Postboks 1105 - Blindern
NO-0317 Oslo

Voice: +47 228 51159
Fax: +47 228 51499

WWW: folk.uio.no/jhelm