Sealing cover slips
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Does anybody know of a non toxic seal for coverslips? I am looking at live-dead stained bacteria (hoping to see them die) but I think that my agarose is shinking due to evaporation over time. I am worried that nail polish or vacuum grease will affect my results. As usual, thanks for any help! Mike |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** We seal them for live cell work with Valap- equal parts vaseline, paraffin wax, lanolin heated on a hot plate at 100C and applied with a fine art brush. Dave On Sep 18, 2012, at 4:42 PM, Mike Tighe wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Does anybody know of a non toxic seal for coverslips? I am looking at live-dead stained bacteria (hoping to see them die) but I think that my agarose is shinking due to evaporation over time. I am worried that nail polish or vacuum grease will affect my results. As usual, thanks for any help! Mike Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax) |
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In reply to this post by Mike Tighe
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Mike, Try VALAP (a 1:1:1 mixture of vaselin, lanolin, and paraffin) or simply use a thin silicone rubber gasket that will adhere to glass quite well with gentle pressure. Jake > Date: Tue, 18 Sep 2012 20:42:58 +0000 > From: [hidden email] > Subject: Sealing cover slips > To: [hidden email] > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Does anybody know of a non toxic seal for coverslips? I am looking at live-dead stained bacteria (hoping to see them die) but I think that my agarose is shinking due to evaporation over time. I am worried that nail polish or vacuum grease will affect my results. > > > > As usual, thanks for any help! > > Mike |
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In reply to this post by Mike Tighe
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I always use wax in this situation. Of course it's easy for me since the EM lab always has a dish of molten wax at hand (it's used for sealing the water-bath on glass microtome knives.) But it completely avoids the toxic effects of the solvents in nail polish. Guy -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Mike Tighe Sent: Wednesday, 19 September 2012 6:43 AM To: [hidden email] Subject: Sealing cover slips ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Does anybody know of a non toxic seal for coverslips? I am looking at live-dead stained bacteria (hoping to see them die) but I think that my agarose is shinking due to evaporation over time. I am worried that nail polish or vacuum grease will affect my results. As usual, thanks for any help! Mike |
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In reply to this post by Mike Tighe
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** This is a non-commercial post. In our lab we use an organic coverslip sealer from the company Biotium called CoverGrip. It comes in the same kind of container as nail polish, with a little brush attached to the lid for ease of use. I've had great luck using this product and the price is extremely fair for a RTU reagent, especially if you don't have the ingredients to make a custom cocktail. Definition from the site: CoverGrip Coverslip Sealant is made with d-limonene, a natural, environmentally-friendly solvent with a pleasant citrus aroma, and dries to form a clear, hard, durable seal along the edges of the coverslip. http://biotium.com/product/product_types/search/price_and_info.asp? item=23005 |
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In reply to this post by Mike Tighe
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Mike, I have successfully used a low temp glue gun and instead of glue, I use Crayola crayons. The crayons melt at the same temp as glue and the melted crayon seals the edge very well with a little practice. They're non-toxic and "color-coded". Contact me for details. Cheers, Mark **************************************************** Mark A. Sanders University of Minnesota Program Director Twin Cities Campus University Imaging Centers St. Paul office ph: 612-624-3454 Mpls office ph: 612-626-3645 fax: 612-624-1799 www.uic.umn.edu On Sep 18, 2012, at 3:42 PM, Mike Tighe wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Does anybody know of a non toxic seal for coverslips? I am looking at live-dead stained bacteria (hoping to see them die) but I think that my agarose is shinking due to evaporation over time. I am worried that nail polish or vacuum grease will affect my results. > > > > As usual, thanks for any help! > > Mike |
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In reply to this post by Chris Booth
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Something we've used on occasion is 2-part dental silicone (the same stuff that is used for making impression moulds) - the exact name and supplier will vary depending on where you are. You mix the parts (I tend to use a small weighing dish to mix in), and apply a bead round the edge of the coverslip (I usually use a pippette tip for this). I've mostly used it for fixed cells, but have heard it touted as being good for live cells (if you can put it in your mouth, it can't be particularly toxic) . It also sets slightly faster than nail-polish, which is helpful if you are under time pressure. cheers, David ________________________________ From: Chris Booth <[hidden email]> To: [hidden email] Sent: Wednesday, 19 September 2012 8:58 AM Subject: Re: Sealing cover slips ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** This is a non-commercial post. In our lab we use an organic coverslip sealer from the company Biotium called CoverGrip. It comes in the same kind of container as nail polish, with a little brush attached to the lid for ease of use. I've had great luck using this product and the price is extremely fair for a RTU reagent, especially if you don't have the ingredients to make a custom cocktail. Definition from the site: CoverGrip Coverslip Sealant is made with d-limonene, a natural, environmentally-friendly solvent with a pleasant citrus aroma, and dries to form a clear, hard, durable seal along the edges of the coverslip. http://biotium.com/product/product_types/search/price_and_info.asp? item=23005 |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** We use Sylgard, you can probably find it a neurobiology lab that does patch clam they use it to seal the patch electrodes. Its non toxic and easy to use. Naomi Book -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of David Baddeley Sent: Wednesday, September 19, 2012 12:36 AM To: [hidden email] Subject: Re: Sealing cover slips ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Something we've used on occasion is 2-part dental silicone (the same stuff that is used for making impression moulds) - the exact name and supplier will vary depending on where you are. You mix the parts (I tend to use a small weighing dish to mix in), and apply a bead round the edge of the coverslip (I usually use a pippette tip for this). I've mostly used it for fixed cells, but have heard it touted as being good for live cells (if you can put it in your mouth, it can't be particularly toxic) . It also sets slightly faster than nail-polish, which is helpful if you are under time pressure. cheers, David ________________________________ From: Chris Booth <[hidden email]> To: [hidden email] Sent: Wednesday, 19 September 2012 8:58 AM Subject: Re: Sealing cover slips ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** This is a non-commercial post. In our lab we use an organic coverslip sealer from the company Biotium called CoverGrip. It comes in the same kind of container as nail polish, with a little brush attached to the lid for ease of use. I've had great luck using this product and the price is extremely fair for a RTU reagent, especially if you don't have the ingredients to make a custom cocktail. Definition from the site: CoverGrip Coverslip Sealant is made with d-limonene, a natural, environmentally-friendly solvent with a pleasant citrus aroma, and dries to form a clear, hard, durable seal along the edges of the coverslip. http://biotium.com/product/product_types/search/price_and_info.asp? item=23005 |
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In reply to this post by Mike Tighe
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Mike, I like using Carolina Observation Gel (http://www.carolina.com/product/life+science/microscope+slides/protist+slides/carolina+observation+gel.do, no commercial interest). It is mouldable and non-toxic polydimethylsiloxane, which can be made into custom gaskets to seal slides (I cut them to shape with a needle). It allows gas exchange, but minimises evaporation. Best wishes, George ****************************** Dr. George Littlejohn School of Biosciences, University of Exeter, Halpin Laboratory, Geoffrey Pope Building, Stocker Road, EX4 4QD, UK ****************************** Tel: +44(0)1392 269170 (Lab.) +44(0)1392 269297 (Office) Fax: +44(0)1392 263434 E-mail: [hidden email] ****************************** http://www.illuminatedcell.com/improved-imaging.html http://projects.exeter.ac.uk/ein/ On 18 Sep 2012, at 21:44, "Mike Tighe" <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Does anybody know of a non toxic seal for coverslips? I am looking at live-dead stained bacteria (hoping to see them die) but I think that my agarose is shinking due to evaporation over time. I am worried that nail polish or vacuum grease will affect my results. > > > > As usual, thanks for any help! > > Mike |
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In reply to this post by Mike Tighe
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi, I don't know it can be funny but one scientist at JNU, India uses feviquick to seal and he has validated that feviquick does not interfere in imaging or viability.I myself have never done this. On Wed, Sep 19, 2012 at 2:12 AM, Mike Tighe <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Does anybody know of a non toxic seal for coverslips? I am looking at > live-dead stained bacteria (hoping to see them die) but I think that my > agarose is shinking due to evaporation over time. I am worried that nail > polish or vacuum grease will affect my results. > > > > As usual, thanks for any help! > > Mike > -- regards Ruchi jain, Graduate student, Professor Deepak Saini Lab, Department of MRDG, IISc,Bangalore, Email id: [hidden email] Contact no.080-22932574 |
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In reply to this post by David Baddeley
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Further to David's email - we also use something similar, a 2-part dental silicone. The product is from PicoDent and called TwinSil ( http://www.picodent.de/artikel/831-picodent_twinsilsupsup_22.html?anz=0) and our local Leica people were able to help us get hold of some after we saw them using it for sample prep on the GSDIM system (no commercial interest in either). After you mix the two solutions you have a few minutes to use it before it sets and seals. The nice thing is that it peals off quite easily afterwards if you have to retrieve the coverslip for something else. Regards, Graham -- Dr Graham Wright Microscopy Unit Manager Institute of Medical Biology 8A Biomedical Grove, #06-06 Immunos, Singapore 138648 E: [hidden email] W: http://www.imb.a-star.edu.sg/imu/ On 19 September 2012 05:35, David Baddeley <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Something we've used on occasion is 2-part dental silicone (the same stuff > that is used for making impression moulds) - the exact name and supplier > will vary depending on where you are. You mix the parts (I tend to use a > small weighing dish to mix in), and apply a bead round the edge of the > coverslip (I usually use a pippette tip for this). I've mostly used it for > fixed cells, but have heard it touted as being good for live cells (if you > can put it in your mouth, it can't be particularly toxic) . It also sets > slightly faster than nail-polish, which is helpful if you are under time > pressure. > > cheers, > David > > > ________________________________ > From: Chris Booth <[hidden email]> > To: [hidden email] > Sent: Wednesday, 19 September 2012 8:58 AM > Subject: Re: Sealing cover slips > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > This is a non-commercial post. In our lab we use an organic coverslip > sealer > from the company Biotium called CoverGrip. It comes in the same kind of > container as nail polish, with a little brush attached to the lid for ease > of use. > I've had great luck using this product and the price is extremely fair for > a RTU > reagent, especially if you don't have the ingredients to make a custom > cocktail. > > Definition from the site: CoverGrip Coverslip Sealant is made with > d-limonene, a > natural, environmentally-friendly solvent with a pleasant citrus aroma, > and dries > to form a clear, hard, durable seal along the edges of the coverslip. > > http://biotium.com/product/product_types/search/price_and_info.asp? > item=23005 > |
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