Silcate coatings
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Hi, I am investigating methods to visualise metasilicate coatings on metal. Does anyone have any ideas on how to image the coating using confocal microscopy? Reflection doesn't work as the coating layer is too thin and the metal surface uneven. I thought possible mixing a dextran probe in with the coating solution but wondered if anyone had any experience or ideas for staining the coating once it had dried onto the metal surface? Liz |
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I do think you'd need to tell us a bit more about your metasilicate coating - its properties, expected thickness and how it is prepared. Having said that, though, my best guess would be that second harmonic might be a first line of attack.
Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope Unit, Madsen Building F09, University of Sydney, NSW 2006 ______________________________________________ Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net <http://www.guycox.net/> ________________________________ From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Elizabeth Nickless Sent: Monday, 23 March 2009 11:10 AM To: [hidden email] Subject: Silcate coatings Hi, I am investigating methods to visualise metasilicate coatings on metal. Does anyone have any ideas on how to image the coating using confocal microscopy? Reflection doesn't work as the coating layer is too thin and the metal surface uneven. I thought possible mixing a dextran probe in with the coating solution but wondered if anyone had any experience or ideas for staining the coating once it had dried onto the metal surface? Liz No virus found in this incoming message. Checked by AVG. Version: 7.5.557 / Virus Database: 270.11.24/2017 - Release Date: 22/03/2009 5:51 PM No virus found in this outgoing message. Checked by AVG. Version: 7.5.557 / Virus Database: 270.11.24/2017 - Release Date: 22/03/2009 5:51 PM |
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In reply to this post by Elizabeth Nickless
I would think that this might be a good application
for AFM (Atomic Force Microscopy).
Joe Goodhouse
Visit us at http://www.molbio1.princeton.edu/facility/confocal/ From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Elizabeth Nickless Sent: Sunday, March 22, 2009 8:10 PM To: [hidden email] Subject: Silcate coatings Hi, I am investigating methods to visualise metasilicate coatings on metal. Does anyone have any ideas on how to image the coating using confocal microscopy? Reflection doesn't work as the coating layer is too thin and the metal surface uneven. I thought possible mixing a dextran probe in with the coating solution but wondered if anyone had any experience or ideas for staining the coating once it had dried onto the metal surface? Liz |
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I
think there are well developed methods to measure thin films using interference
microscopy. Mike From: Confocal Microscopy List
[mailto:[hidden email]] On Behalf Of Elizabeth
Nickless Hi, I am
investigating methods to visualise metasilicate coatings on metal. Does
anyone have any ideas on how to image the coating using confocal
microscopy? Reflection doesn't work as the coating layer is too thin and
the metal surface uneven. I thought possible mixing a dextran probe in
with the coating solution but wondered if anyone had any experience or ideas
for staining the coating once it had dried onto the metal surface? Liz |
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In reply to this post by Guy Cox
The coating is an aqueous solution of disodium metasilicate and sodium
polyphosphate, stainless steel discs (approx.70mm diameter) are soaked in the solution and then let dry. The thickness of the coating is estimated to be around 1um but we are not 100% sure. (we can use smaller pieces of stainless steel if needed) I have been given advice from another member that back scattered SEM might be the way to do it. We need to image large areas across the disc so I thought AFM would not be that practical and I am not sure what the Interference microscopy application mentioned is? we cannot use transmission systems, also from Guy Cox 2nd Harmonics?? I only vaguely remember you talking about this at the microscopy conference in NZ sorry so I am not sure how this application works? Liz -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Guy Cox Sent: Monday, 23 March 2009 10:57 p.m. To: [hidden email] Subject: Re: Silcate coatings I do think you'd need to tell us a bit more about your metasilicate coating - its properties, expected thickness and how it is prepared. Having said that, though, my best guess would be that second harmonic might be a first line of attack. Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope Unit, Madsen Building F09, University of Sydney, NSW 2006 ______________________________________________ Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net <http://www.guycox.net/> ________________________________ From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Elizabeth Nickless Sent: Monday, 23 March 2009 11:10 AM To: [hidden email] Subject: Silcate coatings Hi, I am investigating methods to visualise metasilicate coatings on metal. Does anyone have any ideas on how to image the coating using confocal microscopy? Reflection doesn't work as the coating layer is too thin and the metal surface uneven. I thought possible mixing a dextran probe in with the coating solution but wondered if anyone had any experience or ideas for staining the coating once it had dried onto the metal surface? Liz No virus found in this incoming message. Checked by AVG. Version: 7.5.557 / Virus Database: 270.11.24/2017 - Release Date: 22/03/2009 5:51 PM No virus found in this outgoing message. Checked by AVG. Version: 7.5.557 / Virus Database: 270.11.24/2017 - Release Date: 22/03/2009 5:51 PM DISCLAIMER: This email contains confidential information and may be legally privileged. If you are not the intended recipient or have received this email in error, please notify the sender immediately and destroy this email. You may not use, disclose or copy this email or its attachments in any way. Any opinions expressed in this email are those of the author and are not necessarily those of the Fonterra Co-operative Group. http://www.fonterra.com/ |
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Liz-
you really could incorporate a fluorescent tracer such as TRITC-dextran into the mixture. It is a bit tricky getting the calibration sample but at least a known path length, such as you have in a Neuebauer chamber, should get you a sort of "ballpark" measure of how much dye is in your sample after drying. Carol Heckman Center for Microscopy & Microanalysis Bowling Green State University ________________________________________ From: Confocal Microscopy List [[hidden email]] On Behalf Of Elizabeth Nickless [[hidden email]] Sent: Monday, March 23, 2009 4:31 PM To: [hidden email] Subject: Re: Silcate coatings The coating is an aqueous solution of disodium metasilicate and sodium polyphosphate, stainless steel discs (approx.70mm diameter) are soaked in the solution and then let dry. The thickness of the coating is estimated to be around 1um but we are not 100% sure. (we can use smaller pieces of stainless steel if needed) I have been given advice from another member that back scattered SEM might be the way to do it. We need to image large areas across the disc so I thought AFM would not be that practical and I am not sure what the Interference microscopy application mentioned is? we cannot use transmission systems, also from Guy Cox 2nd Harmonics?? I only vaguely remember you talking about this at the microscopy conference in NZ sorry so I am not sure how this application works? Liz -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Guy Cox Sent: Monday, 23 March 2009 10:57 p.m. To: [hidden email] Subject: Re: Silcate coatings I do think you'd need to tell us a bit more about your metasilicate coating - its properties, expected thickness and how it is prepared. Having said that, though, my best guess would be that second harmonic might be a first line of attack. Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope Unit, Madsen Building F09, University of Sydney, NSW 2006 ______________________________________________ Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net <http://www.guycox.net/> ________________________________ From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Elizabeth Nickless Sent: Monday, 23 March 2009 11:10 AM To: [hidden email] Subject: Silcate coatings Hi, I am investigating methods to visualise metasilicate coatings on metal. Does anyone have any ideas on how to image the coating using confocal microscopy? Reflection doesn't work as the coating layer is too thin and the metal surface uneven. I thought possible mixing a dextran probe in with the coating solution but wondered if anyone had any experience or ideas for staining the coating once it had dried onto the metal surface? Liz No virus found in this incoming message. Checked by AVG. Version: 7.5.557 / Virus Database: 270.11.24/2017 - Release Date: 22/03/2009 5:51 PM No virus found in this outgoing message. Checked by AVG. Version: 7.5.557 / Virus Database: 270.11.24/2017 - Release Date: 22/03/2009 5:51 PM DISCLAIMER: This email contains confidential information and may be legally privileged. If you are not the intended recipient or have received this email in error, please notify the sender immediately and destroy this email. You may not use, disclose or copy this email or its attachments in any way. Any opinions expressed in this email are those of the author and are not necessarily those of the Fonterra Co-operative Group. http://www.fonterra.com/ |
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