Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalHi Elaine,
The normalization occurs in the display, and does not affect the
intensities saved to the native Slidebook file. Most acquisition
software does this, some automatically set the exposures so that the
images themselves are falsely bright. I've not found a preferences
setting in Slidebook for a non-stretched default view. However, if
you open the normalization window, the lower button will normalize
all channels to the lowest and brightest intensities, preserving the
relative intensities in an exported RGB TIFF, although this does not
set all channels to 0 and 4095. If you were wanting to export the
Slide files for deconvolution or densitometric analysis in some other
application, you would export in 16-bit which uses native intensities
without any histogram stretch.
Regards,
Glen
On Sep 8, 2008, at 2:31 PM, Elaine Kunze wrote:
> Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal> Does anyone successfully and happily use Olympus' Slidebook? We
> have had a devil of a time with automatic image normalization
> (making controls look as bright as positives), merging images and
> just image file handling. Any expert help would be greatly
> appreciated. We are just hating this program.
> Elaine Kunze
> Cytometry Facility
> Huck Institute of the Life Sciences
> 319 Life Sciences Building
> Penn State University
> University Park, PA 16802
>
http://www.huck.psu.edu/facilities/cytometry-up/> 814-863-2762
>
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
[hidden email]
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