Sources for fluorescent probes
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hi Folks, I am in need of a comprehensive list of vendors, large and small, of fluorescent probes. At this time I am particularly interested in fluorophores which can be conjugated directly to oligonucleotides for FISH. I am also in need of the same kind of list of companies that perform custom oligonucleotide synthesis and conjugation. Can anyone provide a source for these lists? If none are available I would be prepared to start a list. Therefore if you are a researcher who buys fluorophores or oligonucleotides, would you be willing to offer recommendations for sources? If you are a vendor, I would love to hear from you, as well. If people are interested I am happy to share this with the confocal list. I’ll start this list off with Molecular Probes/Invitrogen, Carlsbad, CA (www.probes.com). Best Regards, Alex _____________________________________ Alex Valm Graduate Student IMD/ADLC The Marine Biological Laboratory 7 MBL St., Woods Hole, MA 02543, USA |
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Alex, While this list isn't specific to fluorescent probes, it is a pretty comprehensive list antibody manufacturers, See: Nature 447:745-745 (7 June 2007) If you have institutional access to this journal, a direct link to the PDF is here: http://www.nature.com/nature/journal/v447/n7145/pdf/447745a.pdf Doug Alex Valm wrote: > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > Hi Folks, > > I am in need of a comprehensive list of vendors, large and small, of > fluorescent probes. At this time I am particularly interested in > fluorophores which can be conjugated directly to oligonucleotides for > FISH. I am also in need of the same kind of list of companies that > perform custom oligonucleotide synthesis and conjugation. Can anyone > provide a source for these lists? > > If none are available I would be prepared to start a list. Therefore > if you are a researcher who buys fluorophores or oligonucleotides, > would you be willing to offer recommendations for sources? If you are > a vendor, I would love to hear from you, as well. If people are > interested I am happy to share this with the confocal list. > > I’ll start this list off with Molecular Probes/Invitrogen, Carlsbad, > CA (www.probes.com <http://www.probes.com>). > > Best Regards, > > Alex > > _____________________________________ > > Alex Valm > Graduate Student > IMD/ADLC > The Marine Biological Laboratory > 7 MBL St., Woods Hole, MA 02543, USA > [hidden email] <mailto:[hidden email]> > > > -- ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ Douglas W. Cromey, M.S. - Assistant Scientific Investigator Dept. of Cell Biology & Anatomy, University of Arizona 1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA office: AHSC 4212 email: [hidden email] voice: 520-626-2824 fax: 520-626-2097 http://swehsc.pharmacy.arizona.edu/exppath/ Home of: "Microscopy and Imaging Resources on the WWW" |
 
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Craig Brideau |
Re: Sources for fluorescent probes
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In reply to this post by Alex Valm
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal A company called Visen Medical makes some very interesting Near-IR dyes. You pump them with red light, they emit NIR. They penetrate tissue extremely well, but of course you need the filters and detectors capable of detecting NIR light. Craig On 9/26/07, Alex Valm <[hidden email]> wrote: > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > > Hi Folks, > > I am in need of a comprehensive list of vendors, large and small, of > fluorescent probes. At this time I am particularly interested in > fluorophores which can be conjugated directly to oligonucleotides for FISH. > I am also in need of the same kind of list of companies that perform custom > oligonucleotide synthesis and conjugation. Can anyone provide a source for > these lists? > > If none are available I would be prepared to start a list. Therefore if you > are a researcher who buys fluorophores or oligonucleotides, would you be > willing to offer recommendations for sources? If you are a vendor, I would > love to hear from you, as well. If people are interested I am happy to > share this with the confocal list. > > I'll start this list off with Molecular Probes/Invitrogen, Carlsbad, CA > (www.probes.com). > > Best Regards, > > Alex > > _____________________________________ > Alex Valm > Graduate Student > IMD/ADLC > The Marine Biological Laboratory > 7 MBL St., Woods Hole, MA 02543, USA > [hidden email] > > > > |
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Barbara Foster |
Re: Sources for fluorescent probes
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Dear Listers,
The NIR has been a long neglected but useful part of the spectrum. In the early days, I had a chance to work with the PolyVar InfraPol from Reichert-Jung (aka, American Optical/Cambridge Instruments/now Leica). It turns out that most microscope objectives have good to excellent transmission properties throughout this spectral range, usually to about 2200nm (NIR is often done in the 1100-1300 range). The big challenge is the camera. If a heat absorbing filter has been added, it will absorb everything beyond about 800, so it is worthwhile discussing the chip's responsiveness with your camera provider. Ditto for the filters in your fluorescence cube. Both the microscope and the filter manufacturers can provide cube sets to optimize transmission in this area. One other caveat. Remember that there is a wavelength component in Rayleigh's resolution equation. Although not often discussed, because resolution is based on diffraction, this equation not only determines how small a feature you will be able to resolve, but also edge fidelity, so don't expect the same level of image sharpness in the NIR as you enjoy in the visible range of the spectrum. Fluorescence is more detection limited than diffraction limited, but this factor still affects image quality. Hope this was helpful, Best regards, Barbara Foster, President We've moved! Microscopy/Microscopy Education 7101 Royal Glen Trail, Suite A McKinney TX 75070 P: (972)924-5310 Skype: fostermme W: www.MicroscopyEducation.com MME is now scheduling customized, on-site courses through December. Call us today for details. P. S. Need a good general reference or light microscopy text for next semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Just email Ken Piel here in the MME office for details ([hidden email]). At 01:14 PM 9/26/2007, Craig Brideau wrote: Search the CONFOCAL archive at |
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George McNamara |
Re: Sources for fluorescent probes
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In reply to this post by Craig Brideau
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal The Visen dyes are rebranded Alexa Fluor ###'s. At 01:04 PM 9/26/2007, you wrote: >Search the CONFOCAL archive at >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >A company called Visen Medical makes some very interesting Near-IR >dyes. You pump them with red light, they emit NIR. They penetrate >tissue extremely well, but of course you need the filters and >detectors capable of detecting NIR light. > >Craig > >On 9/26/07, Alex Valm <[hidden email]> wrote: > > Search the CONFOCAL archive at > > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > > > > > Hi Folks, > > > > I am in need of a comprehensive list of vendors, large and small, of > > fluorescent probes. At this time I am particularly interested in > > fluorophores which can be conjugated directly to oligonucleotides for FISH. > > I am also in need of the same kind of list of companies that perform custom > > oligonucleotide synthesis and conjugation. Can anyone provide a source for > > these lists? > > > > If none are available I would be prepared to start a list. Therefore if you > > are a researcher who buys fluorophores or oligonucleotides, would you be > > willing to offer recommendations for sources? If you are a vendor, I would > > love to hear from you, as well. If people are interested I am happy to > > share this with the confocal list. > > > > I'll start this list off with Molecular Probes/Invitrogen, Carlsbad, CA > > (www.probes.com). > > > > Best Regards, > > > > Alex > > > > _____________________________________ > > Alex Valm > > Graduate Student > > IMD/ADLC > > The Marine Biological Laboratory > > 7 MBL St., Woods Hole, MA 02543, USA > > [hidden email] > > > > > > > > George McNamara, Ph.D. University of Miami, Miller School of Medicine Image Core Miami, FL 33010 [hidden email] [hidden email] 305-243-8436 office |
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George McNamara |
Re: Sources for fluorescent probes
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In reply to this post by Alex Valm
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
One of the columns in McNamara 2007 Fluorophore Data Tables (if the web link does not work in your email, you can go to http://home.earthlink.net/~pubspectra/ and download it from there) has the manufacturer name or web link. Copy the column to a new Excel sheet, sort, get rid of duplicates. As for labeling oligo's, check out http://www.biosearchtech.com/products/display.asp?catID=40 and http://www.sigmaaldrich.com/Brands/Sigma_Genosys/Custom_DNA/Product_Lines/Fluorescent_Oligos.html At 12:13 PM 9/26/2007, you wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal George McNamara, Ph.D. University of Miami, Miller School of Medicine Image Core Miami, FL 33010 [hidden email] [hidden email] 305-243-8436 office |
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In reply to this post by George McNamara
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Just out of curiosity, why would MP/Invitrogen allow "rebranding" of such successful patented products? >Search the CONFOCAL archive at >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >The Visen dyes are rebranded Alexa Fluor ###'s. > >At 01:04 PM 9/26/2007, you wrote: >>Search the CONFOCAL archive at >>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >> >>A company called Visen Medical makes some very interesting Near-IR >>dyes. You pump them with red light, they emit NIR. They penetrate >>tissue extremely well, but of course you need the filters and >>detectors capable of detecting NIR light. >> >>Craig >> >>On 9/26/07, Alex Valm <[hidden email]> wrote: >>> Search the CONFOCAL archive at >>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >>> >>> >>> Hi Folks, >>> >>> I am in need of a comprehensive list of vendors, large and small, of >>> fluorescent probes. At this time I am particularly interested in >>> fluorophores which can be conjugated directly to oligonucleotides for FISH. >>> I am also in need of the same kind of list of companies that perform custom >>> oligonucleotide synthesis and conjugation. Can anyone provide a source for >>> these lists? >>> >>> If none are available I would be prepared to start a list. Therefore if you >>> are a researcher who buys fluorophores or oligonucleotides, would you be >>> willing to offer recommendations for sources? If you are a vendor, I would >>> love to hear from you, as well. If people are interested I am happy to >>> share this with the confocal list. >>> >>> I'll start this list off with Molecular Probes/Invitrogen, Carlsbad, CA >>> (www.probes.com). >>> >>> Best Regards, >>> >>> Alex >>> >>> _____________________________________ >>> Alex Valm >>> Graduate Student >>> IMD/ADLC >>> The Marine Biological Laboratory >>> 7 MBL St., Woods Hole, MA 02543, USA >>> [hidden email] >>> >>> >>> >>> > > > > > > >George McNamara, Ph.D. >University of Miami, Miller School of Medicine >Image Core >Miami, FL 33010 >[hidden email] >[hidden email] >305-243-8436 office -- Robert J. Palmer Jr., Ph.D. Natl Inst Dental Craniofacial Res - Natl Insts Health Oral Infection and Immunity Branch Bldg 30, Room 310 30 Convent Drive Bethesda MD 20892 ph 301-594-0025 fax 301-402-0396 |
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David Barnes-2 |
Re: Sources for fluorescent probes
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In reply to this post by Barbara Foster
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Barbara, you must remember when John Bogan of QImaging introduced the QICAMIR, now the RoleraXR, esp. designed for NIR work. Plus many (most) high end Sony 285 cameras have a high QE mode, which shifts the sensitivity spectrum toward the red range. I know of one lab in Pittsburgh which routinely images at 1000nm with a QImaging camera.
On 9/26/07, Barbara Foster <[hidden email]> wrote:
Search the CONFOCAL archive at <a onclick="return top.js.OpenExtLink(window,event,this)" href="http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal" target="_blank"> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal |
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Jos de Lange |
Re: Sources for fluorescent probes
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In reply to this post by rjpalmer
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal I believe that there is some misinformation. VisEn Medical probes are proprietary molecules and they have developed many of the dyes in-house. Jos "Robert J. Palmer Jr." <[hidden email] To CR.NIH.GOV> [hidden email] Sent by: Confocal cc Microscopy List <CONFOCAL@LISTSER Subject V.BUFFALO.EDU> Re: Sources for fluorescent probes 09/27/2007 08:05 PM Please respond to Confocal Microscopy List <CONFOCAL@LISTSER V.BUFFALO.EDU> Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Just out of curiosity, why would MP/Invitrogen allow "rebranding" of such successful patented products? >Search the CONFOCAL archive at >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >The Visen dyes are rebranded Alexa Fluor ###'s. > >At 01:04 PM 9/26/2007, you wrote: >>Search the CONFOCAL archive at >>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >> >>A company called Visen Medical makes some very interesting Near-IR >>dyes. You pump them with red light, they emit NIR. They penetrate >>tissue extremely well, but of course you need the filters and >>detectors capable of detecting NIR light. >> >>Craig >> >>On 9/26/07, Alex Valm <[hidden email]> wrote: >>> Search the CONFOCAL archive at >>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >>> >>> >>> Hi Folks, >>> >>> I am in need of a comprehensive list of vendors, large and small, of >>> fluorescent probes. At this time I am particularly interested in >>> fluorophores which can be conjugated directly to oligonucleotides for >>> I am also in need of the same kind of list of companies that perform custom >>> oligonucleotide synthesis and conjugation. Can anyone provide a source for >>> these lists? >>> >>> If none are available I would be prepared to start a list. Therefore if you >>> are a researcher who buys fluorophores or oligonucleotides, would you be >>> willing to offer recommendations for sources? If you are a vendor, I would >>> love to hear from you, as well. If people are interested I am happy to >>> share this with the confocal list. >>> >>> I'll start this list off with Molecular Probes/Invitrogen, Carlsbad, CA >>> (www.probes.com). >>> >>> Best Regards, >>> >>> Alex >>> >>> _____________________________________ >>> Alex Valm >>> Graduate Student >>> IMD/ADLC >>> The Marine Biological Laboratory >>> 7 MBL St., Woods Hole, MA 02543, USA >>> [hidden email] >>> >>> >>> >>> > > > > > > >George McNamara, Ph.D. >University of Miami, Miller School of Medicine >Image Core >Miami, FL 33010 >[hidden email] >[hidden email] >305-243-8436 office -- Robert J. Palmer Jr., Ph.D. Natl Inst Dental Craniofacial Res - Natl Insts Health Oral Infection and Immunity Branch Bldg 30, Room 310 30 Convent Drive Bethesda MD 20892 ph 301-594-0025 fax 301-402-0396 |
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Carol Bayles |
NERLSCD - Regional Core Directors Meeting on Nov. 7-9, 2007
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear Core Directors and Managers, We would like to invite you to the second annual "Northeast Regional Life Sciences Core Directors" (NERLSCD) meeting, Nov. 7-9, 2007, at Cornell University in Ithaca, NY. This is a networking meeting for core directors and managers in the northeastern area of the United States. There will be a reception on the evening of Wednesday, Nov. 7th, followed by a two day meeting from Thursday to Friday. The meeting will include plenary presentations of general interest to all core directors, a poster session (the posters will describe the activities and services provided by the core facilities of the participants), and roundtable discussion sessions on DNA Sequencing, Genotyping, Microarrays, Quantitative PCR, Proteomics and Mass Spectrometry, Protein Production and Characterization, Flow Cytometry, Optical Imaging, Transgenics, Outreach, and Bioinformatics and Bio-IT. For more information and registration, please go to http://nerlscd.biotech.cornell.edu. Registration to this meeting will be limited, so please register soon. We look forward to seeing you at the meeting! The Organizing Committee of the NERLSCD 2007 meeting, including George Grills (Cornell University), Michelle Detwiler (Roswell Park Cancer Institute), Tim Hunter (University of Vermont), Stephen Bobin (Dartmouth Medical Center), Katia Sol-Church (NCC-Delaware), Robert Keefe (Wadsworth Center/NYS DOH), Scottie Adams (Trudeau Institute), and Ted Thannhauser (USDA-ARS). -- <><><><><><><><><><><><><><><><><><><><><> Carol Bayles, Manager Microscopy & Imaging Facility (MIF) Life Sciences Core Lab Center 160a Biotech Bldg 607-254-4860 http://cores.lifesciences.cornell.edu Confocal and Multiphoton Microscopy Nanobiotechnology Center www.nbtc.cornell.edu Cornell University Ithaca NY 14853 |
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