Spinning disk with rolling shutter sCMOS cameras

[Csúcs Gábor-3]

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Dear All,

I would very much appreciate some feedback from those who run spinning disk systems with sCMOS cameras with a rolling shutter (like the Flash 4 or similar). Have you experienced any problems? I know of course that your can create imaging condisitions where having a "only" a rolling shutter can be a problem (can create artefacts) but I am wondering whether if in real experimental situations this is indeed an issue.

Thanks for your comments Gabor

[Bálint Balázs]

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Dear Gabor,

Although I haven't used these cameras on a spinning disk system, I have
some experience with them on a light-sheet setup. The Flash4 models have a
global reset mode for the exposure, which means all rows will expose
simultaneously, and only the readout follows the rolling mode. This way
it's possible to avoid the typical imaging artifacts affecting the standard
rolling shutter sensors. One drawback though, is that the exposure and
readout for different rows can't be overlapped, which means the maximum
frame rate is slightly lower.

Best,
Balint

Csucs Gabor <[hidden email]> ezt írta (időpont: 2019. jún. 6.,
Cs, 6:40):

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> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
> I would very much appreciate some feedback from those who run spinning
> disk systems with sCMOS cameras with a rolling shutter (like the Flash 4 or
> similar). Have you experienced any problems? I know of course that your can
> create imaging condisitions where having a "only" a rolling shutter can be
> a problem (can create artefacts) but I am wondering whether if in real
> experimental situations this is indeed an issue.
>
> Thanks for your comments                                Gabor
>

[ICIT]

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Dear Gabor.
I've been using the following sCMOS cameras with Clarity Laser-free Spinning Disk Confocal unit without any problems what so ever:

Hamamatsu: ORCA Flash 4 V2, Flash V3 and Fusion

Photometrics: Prime 95B and Prime BSI

PCO Edge 5.5

Andor Zyla 5.5

Perhaps the unique disk architecture basing on structured grids rather than pinholes plays a role. Also other type of cameras than sCMOS work fine, right now I'm testing Retiga R6 CCD.

Best,

Mika

[leavesley]

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We tried a Prime 95B on our Andor WD spinning disk during an equipment
demo and it seemed to work fine.

Best regards,

Silas

On 6/6/2019 8:44 AM, Mika Ruonala wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Gabor.
> I've been using the following sCMOS cameras with Clarity Laser-free Spinning Disk Confocal unit without any problems what so ever:
>
> Hamamatsu: ORCA Flash 4 V2, Flash V3 and Fusion
>
> Photometrics: Prime 95B and Prime BSI
>
> PCO Edge 5.5
>
> Andor Zyla 5.5
>
> Perhaps the unique disk architecture basing on structured grids rather than pinholes plays a role. Also other type of cameras than sCMOS work fine, right now I'm testing Retiga R6 CCD.
>
> Best,
>
> Mika

[Moritz Armbruster]

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We're using a Prime95B and have tried a Andor Zyla as well on our X-light spinning disk without trouble as well.  We've gone up to 200fps.  I can't say if there are issues if you try to go faster.

Best,
Moritz