Splitter for switching between two cameras on one c-mount?

[Weber, Michael]

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Dear all,

I’m looking for a camera switch/splitter with which I can switch between two cameras mounted on one c-mount. All options I found are dual camera adapters, i.e. running two cameras simultaneously. Instead of a dichroic mirror, all I need is a 4f system with a flip mirror. I’ve seen a simple-looking one on a Zeiss widefield setup recently. A field of view large enough to work for a full sCMOS chip would be great, but not a strict must. Here are the three adapters made for simultaneous dual-camera imaging I found online:

1) Thorlabs two-camera mount (no 4f system, though)
2) Andor dual-camera coupler
3) Cairn TwinCam

Do you know about other options, ideally with a flip mirror?

Best,
Michael

_____________

Dr. Michael Weber
Advanced Microscopy Fellow
Harvard Medical School
240 Longwood Ave, LHRRB 113, Boston, MA 02115
http://nic.med.harvard.edu

[Loralei Dewe-3]

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Yiu didn't mention the type of microscope but most of the big 4 companies
carry them for their higher end models. I put one on an IX-81 recently
purchasing it from Olympus...

Loralei

On Jul 6, 2017 6:56 AM, "Weber, Michael" <[hidden email]>
wrote:

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> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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> *****
>
> Dear all,
>
> I’m looking for a camera switch/splitter with which I can switch between
> two cameras mounted on one c-mount. All options I found are dual camera
> adapters, i.e. running two cameras simultaneously. Instead of a dichroic
> mirror, all I need is a 4f system with a flip mirror. I’ve seen a
> simple-looking one on a Zeiss widefield setup recently. A field of view
> large enough to work for a full sCMOS chip would be great, but not a strict
> must. Here are the three adapters made for simultaneous dual-camera imaging
> I found online:
>
> 1) Thorlabs two-camera mount (no 4f system, though)
> 2) Andor dual-camera coupler
> 3) Cairn TwinCam
>
> Do you know about other options, ideally with a flip mirror?
>
> Best,
> Michael
>
> _____________
>
> Dr. Michael Weber
> Advanced Microscopy Fellow
> Harvard Medical School
> 240 Longwood Ave, LHRRB 113, Boston, MA 02115
> http://nic.med.harvard.edu
>
>

[John Oreopoulos]

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Commercial Response:

Hi Michael,

I'm pretty sure the Andor TuCam can be fitted with a high-quality 100%  
mirror instead of a dichroic at the splitting position allowing one to  
switch between two different cameras for imaging.

Cheers,

John Oreopoulos
Staff Scientist
Andor Technology
www.andor.com



Quoting "Weber, Michael" <[hidden email]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear all,
>
> I?m looking for a camera switch/splitter with which I can switch  
> between two cameras mounted on one c-mount. All options I found are  
> dual camera adapters, i.e. running two cameras simultaneously.  
> Instead of a dichroic mirror, all I need is a 4f system with a flip  
> mirror. I?ve seen a simple-looking one on a Zeiss widefield setup  
> recently. A field of view large enough to work for a full sCMOS chip  
>  would be great, but not a strict must. Here are the three adapters  
> made for simultaneous dual-camera imaging I found online:
>
> 1) Thorlabs two-camera mount (no 4f system, though)
> 2) Andor dual-camera coupler
> 3) Cairn TwinCam
>
> Do you know about other options, ideally with a flip mirror?
>
> Best,
> Michael
>
> _____________
>
> Dr. Michael Weber
> Advanced Microscopy Fellow
> Harvard Medical School
> 240 Longwood Ave, LHRRB 113, Boston, MA 02115
> http://nic.med.harvard.edu
>
>

[Gurjeet Arora]

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Dear Michael,

You may also check on Hamamatsu Gemini 2c
http://www.hamamatsu.com/us/en/A12801-10.html

Regards

Gurjeet Arora


On Jul 6, 2017 7:26 PM, "Weber, Michael" <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear all,
>
> I’m looking for a camera switch/splitter with which I can switch between
> two cameras mounted on one c-mount. All options I found are dual camera
> adapters, i.e. running two cameras simultaneously. Instead of a dichroic
> mirror, all I need is a 4f system with a flip mirror. I’ve seen a
> simple-looking one on a Zeiss widefield setup recently. A field of view
> large enough to work for a full sCMOS chip would be great, but not a strict
> must. Here are the three adapters made for simultaneous dual-camera imaging
> I found online:
>
> 1) Thorlabs two-camera mount (no 4f system, though)
> 2) Andor dual-camera coupler
> 3) Cairn TwinCam
>
> Do you know about other options, ideally with a flip mirror?
>
> Best,
> Michael
>
> _____________
>
> Dr. Michael Weber
> Advanced Microscopy Fellow
> Harvard Medical School
> 240 Longwood Ave, LHRRB 113, Boston, MA 02115
> http://nic.med.harvard.edu
>
>

[Romain Laine]

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Hi Michael,
We built an image splitter out of Thorlabs parts for polarization imaging a
few years ago. You can design it to split at the Fourier plane with a 4f
system quite easily. We built it out of cage system and lens tubes and it
worked well.

Also, we have  TwinCam from Cairn (no commercial interest here) and we are
happy with it (you can also fit a 100% mirror in it to image in the
reflected arm). But I understand this might be an overkill for you here.

Silly question: in our hands, it is easy enough to swap cameras around on
C-mount so why not simply change the cameras over when you need to change
if you do not need simultaneous dual imaging?This might actually perform
better overall since you do not have to deal with your relay being
completely light tight.

All the best,

Romain

--
Dr. Romain Laine, PhD in Biophotonics
Laser Analytics Group
Department of Chemical Engineering and Biotechnology
University of Cambridge
West Cambridge Site
Philippa Fawcett Drive
Cambridge
CB3 0AS


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On Thu, Jul 6, 2017 at 3:06 PM, <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Commercial Response:
>
> Hi Michael,
>
> I'm pretty sure the Andor TuCam can be fitted with a high-quality 100%
> mirror instead of a dichroic at the splitting position allowing one to
> switch between two different cameras for imaging.
>
> Cheers,
>
> John Oreopoulos
> Staff Scientist
> Andor Technology
> www.andor.com
>
>
>
> Quoting "Weber, Michael" <[hidden email]>:
>
> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Dear all,
>>
>> I?m looking for a camera switch/splitter with which I can switch  between
>> two cameras mounted on one c-mount. All options I found are  dual camera
>> adapters, i.e. running two cameras simultaneously.  Instead of a dichroic
>> mirror, all I need is a 4f system with a flip  mirror. I?ve seen a
>> simple-looking one on a Zeiss widefield setup  recently. A field of view
>> large enough to work for a full sCMOS chip  would be great, but not a
>> strict must. Here are the three adapters  made for simultaneous dual-camera
>> imaging I found online:
>>
>> 1) Thorlabs two-camera mount (no 4f system, though)
>> 2) Andor dual-camera coupler
>> 3) Cairn TwinCam
>>
>> Do you know about other options, ideally with a flip mirror?
>>
>> Best,
>> Michael
>>
>> _____________
>>
>> Dr. Michael Weber
>> Advanced Microscopy Fellow
>> Harvard Medical School
>> 240 Longwood Ave, LHRRB 113, Boston, MA 02115
>> http://nic.med.harvard.edu
>>
>>
>>

[Gary Laevsky]

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*****

We have an Andor TuCam with a Flash on one port and an ImagEm on the other,
with different mag optics for each path.  Works well.

On Thu, Jul 6, 2017 at 10:06 AM, <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Commercial Response:
>
> Hi Michael,
>
> I'm pretty sure the Andor TuCam can be fitted with a high-quality 100%
> mirror instead of a dichroic at the splitting position allowing one to
> switch between two different cameras for imaging.
>
> Cheers,
>
> John Oreopoulos
> Staff Scientist
> Andor Technology
> www.andor.com
>
>
>
> Quoting "Weber, Michael" <[hidden email]>:
>
> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Dear all,
>>
>> I?m looking for a camera switch/splitter with which I can switch  between
>> two cameras mounted on one c-mount. All options I found are  dual camera
>> adapters, i.e. running two cameras simultaneously.  Instead of a dichroic
>> mirror, all I need is a 4f system with a flip  mirror. I?ve seen a
>> simple-looking one on a Zeiss widefield setup  recently. A field of view
>> large enough to work for a full sCMOS chip  would be great, but not a
>> strict must. Here are the three adapters  made for simultaneous dual-camera
>> imaging I found online:
>>
>> 1) Thorlabs two-camera mount (no 4f system, though)
>> 2) Andor dual-camera coupler
>> 3) Cairn TwinCam
>>
>> Do you know about other options, ideally with a flip mirror?
>>
>> Best,
>> Michael
>>
>> _____________
>>
>> Dr. Michael Weber
>> Advanced Microscopy Fellow
>> Harvard Medical School
>> 240 Longwood Ave, LHRRB 113, Boston, MA 02115
>> http://nic.med.harvard.edu
>>
>>
>>

--
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310

[Craig Brideau]

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A quick-change bayonet mount and plate on each camera might get the job
done...

Craig

On Thu, Jul 6, 2017 at 8:29 AM, Romain Laine <[hidden email]> wrote:

> Silly question: in our hands, it is easy enough to swap cameras around on
> C-mount so why not simply change the cameras over when you need to change
> if you do not need simultaneous dual imaging?This might actually perform
> better overall since you do not have to deal with your relay being
> completely light tight.
>
> All the best,
>
> Romain
>
> --
> Dr. Romain Laine, PhD in Biophotonics
> Laser Analytics Group
> Department of Chemical Engineering and Biotechnology
> University of Cambridge
> West Cambridge Site
> Philippa Fawcett Drive
> Cambridge
> CB3 0AS
>
>
> <https://www.avast.com/sig-email?utm_medium=email&utm_
> source=link&utm_campaign=sig-email&utm_content=webmail>
> Virus-free.
> www.avast.com
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> source=link&utm_campaign=sig-email&utm_content=webmail>
> <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2>
>
> On Thu, Jul 6, 2017 at 3:06 PM, <[hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Commercial Response:
> >
> > Hi Michael,
> >
> > I'm pretty sure the Andor TuCam can be fitted with a high-quality 100%
> > mirror instead of a dichroic at the splitting position allowing one to
> > switch between two different cameras for imaging.
> >
> > Cheers,
> >
> > John Oreopoulos
> > Staff Scientist
> > Andor Technology
> > www.andor.com
> >
> >
> >
> > Quoting "Weber, Michael" <[hidden email]>:
> >
> > *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >> Post images on http://www.imgur.com and include the link in your
> posting.
> >> *****
> >>
> >> Dear all,
> >>
> >> I?m looking for a camera switch/splitter with which I can switch
> between
> >> two cameras mounted on one c-mount. All options I found are  dual camera
> >> adapters, i.e. running two cameras simultaneously.  Instead of a
> dichroic
> >> mirror, all I need is a 4f system with a flip  mirror. I?ve seen a
> >> simple-looking one on a Zeiss widefield setup  recently. A field of view
> >> large enough to work for a full sCMOS chip  would be great, but not a
> >> strict must. Here are the three adapters  made for simultaneous
> dual-camera
> >> imaging I found online:
> >>
> >> 1) Thorlabs two-camera mount (no 4f system, though)
> >> 2) Andor dual-camera coupler
> >> 3) Cairn TwinCam
> >>
> >> Do you know about other options, ideally with a flip mirror?
> >>
> >> Best,
> >> Michael
> >>
> >> _____________
> >>
> >> Dr. Michael Weber
> >> Advanced Microscopy Fellow
> >> Harvard Medical School
> >> 240 Longwood Ave, LHRRB 113, Boston, MA 02115
> >> http://nic.med.harvard.edu
> >>
> >>
> >>
>

[Kyle Michael Douglass]

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Hi Romain,


 > We built an image splitter out of Thorlabs parts for polarization
imaging a
 > few years ago. You can design it to split at the Fourier plane with a 4f
 > system quite easily. We built it out of cage system and lens tubes
and it
 > worked well.

Slightly off-topic from the original question, but related: is there a
reason why you split the channels at the Fourier plane of your 4f
system? I can imagine that there may be advantages to placing the
dichroic elsewhere in the infinity space, such as immediately before the
Fourier plane. This should essentially give you two independent copies
of the pupil (one for each camera).

I am just curious whether there is an optimum position of the dichroic
for minimizing the aberrations imparted on the split images.

Cheers,
Kyle

--
Kyle M. Douglass, PhD
Post-doctoral researcher
The Laboratory of Experimental Biophysics
EPFL, Lausanne, Switzerland
http://kmdouglass.github.io
http://leb.epfl.ch

[Romain Laine]

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Hi Kyle,
No there isn't really. As you mentioned, having access to the two separate
pupil is a good reason to split them right before the Fourier plane.
As long as the optical component is not anywhere near an image plane (so
typically somewhere in the infinity space) it should be fine.
It could be advantageous to use the FP if it is difficult to manufacture
the optics to insert with large size since the Fourier plane will be where
the beam is smallest (although with very small difference due to the
typically small NAs in the detection side). That's probably not the case
here.

Cheers,

Romain

--
Dr. Romain Laine, PhD in Biophotonics
Laser Analytics Group
Department of Chemical Engineering and Biotechnology
University of Cambridge
West Cambridge Site
Philippa Fawcett Drive
Cambridge
CB3 0AS





--
Dr. Romain Laine, PhD in Biophotonics
Laser Analytics Group
Department of Chemical Engineering and Biotechnology
University of Cambridge
West Cambridge Site
Philippa Fawcett Drive
Cambridge
CB3 0AS

On Fri, Jul 7, 2017 at 9:32 AM, Kyle Douglass <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Romain,
>
>
> > We built an image splitter out of Thorlabs parts for polarization
> imaging a
> > few years ago. You can design it to split at the Fourier plane with a 4f
> > system quite easily. We built it out of cage system and lens tubes and it
> > worked well.
>
> Slightly off-topic from the original question, but related: is there a
> reason why you split the channels at the Fourier plane of your 4f system? I
> can imagine that there may be advantages to placing the dichroic elsewhere
> in the infinity space, such as immediately before the Fourier plane. This
> should essentially give you two independent copies of the pupil (one for
> each camera).
>
> I am just curious whether there is an optimum position of the dichroic for
> minimizing the aberrations imparted on the split images.
>
> Cheers,
> Kyle
>
> --
> Kyle M. Douglass, PhD
> Post-doctoral researcher
> The Laboratory of Experimental Biophysics
> EPFL, Lausanne, Switzerland
> http://kmdouglass.github.io
> http://leb.epfl.ch
>