Staining Plant roots for actin

[Terri Bruce]

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Hi!

Does anyone have any protocols for fixing and staining actin in whole plant roots that works really well?

All suggestions are appreciated!

Thanks!
Terri

Sent from my iPad

[Rosemary.White]

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Check out David Collings' papers. I think this one has intact root double labelling of actin and tubulin:

Collings, D.A. and Wasteneys, G.O. (2005) Actin microfilament and microtubule distribution patterns in the expanding root of Arabidopsis thaliana. Canadian Journal of Botany 83: 579-590
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From: Confocal Microscopy List [[hidden email]] on behalf of Terri Bruce [[hidden email]]
Sent: Friday, 22 November 2013 4:41 p.m.
To: [hidden email]
Subject: Staining Plant roots for actin

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Hi!

Does anyone have any protocols for fixing and staining actin in whole plant roots that works really well?

All suggestions are appreciated!

Thanks!
Terri

Sent from my iPad

[Tobias Baskin]

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Terri,
        In addition to the paper that Rosemary White mentioned, you
can also check:

Rahman et al. 2007 Plant Journal 50: 514-528.   THis protocol is a
bit complicated but I do feel that it works well. If you have
specific q's feel free to email me off line.

Good luck,
        Tobias

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>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>
>Hi!
>
>Does anyone have any protocols for fixing and staining actin in
>whole plant roots that works really well?
>
>All suggestions are appreciated!
>
>Thanks!
>Terri
>
>Sent from my iPad

... [show rest of quote]

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