TagRFP vs mCherry

[lgelman]

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Hi everybody,

 

Has anybody tested TagRFP, and eventually compared it to mCherry?

 

It seems from the literature that TagRFP is much brighter. Is it also
photostable? On our scopes, we have filters matching both proteins, we
just want to pick up the "best" one...

 

Thanks in advance for your feedback!

 

Laurent.

 

 

___________________________

Laurent Gelman, PhD

Friedrich Miescher Institut

Facility for Advanced Imaging and Microscopy

Head of Light Microscopy

WRO 1066.2.16

Maulbeerstrasse 66

CH-4058 Basel

[phone]

 

[Stephen Bunnell]

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You'll want the turbo variant, which is critical for photostability. In our hands that has been much brighter than mCherry. However, we have been restricted to a 568 laser.

Sent from iPhone

On May 23, 2011, at 10:30 AM, "Gelman, Laurent" <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi everybody,
>
>
>
> Has anybody tested TagRFP, and eventually compared it to mCherry?
>
>
>
> It seems from the literature that TagRFP is much brighter. Is it also
> photostable? On our scopes, we have filters matching both proteins, we
> just want to pick up the "best" one...
>
>
>
> Thanks in advance for your feedback!
>
>
>
> Laurent.
>
>
>
>
>
> ___________________________
>
> Laurent Gelman, PhD
>
> Friedrich Miescher Institut
>
> Facility for Advanced Imaging and Microscopy
>
> Head of Light Microscopy
>
> WRO 1066.2.16
>
> Maulbeerstrasse 66
>
> CH-4058 Basel
>
> [phone]
>
>

[Kurt Thorn]

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  I tested TagRFP-T in yeast recently and found that it was less bright
than mCherry.  However, there could be issues arising from different
codon usage in the two constructs - it wasn't as carefully controlled an
experiment as could be done.

Kurt

On 5/23/2011 8:57 AM, Bunnell, Stephen C. wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> You'll want the turbo variant, which is critical for photostability. In our hands that has been much brighter than mCherry. However, we have been restricted to a 568 laser.
>
> Sent from iPhone
>
> On May 23, 2011, at 10:30 AM, "Gelman, Laurent"<[hidden email]>  wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Hi everybody,
>>
>>
>>
>> Has anybody tested TagRFP, and eventually compared it to mCherry?
>>
>>
>>
>> It seems from the literature that TagRFP is much brighter. Is it also
>> photostable? On our scopes, we have filters matching both proteins, we
>> just want to pick up the "best" one...
>>
>>
>>
>> Thanks in advance for your feedback!
>>
>>
>>
>> Laurent.
>>
>>
>>
>>
>>
>> ___________________________
>>
>> Laurent Gelman, PhD
>>
>> Friedrich Miescher Institut
>>
>> Facility for Advanced Imaging and Microscopy
>>
>> Head of Light Microscopy
>>
>> WRO 1066.2.16
>>
>> Maulbeerstrasse 66
>>
>> CH-4058 Basel
>>
>> [phone]
>>
>>

[sprag]

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Laurent,

It depends on the laser you've got.
TagRFP absorbs at ~560, whereas mCherry is at ~590.
My experience is TagRFP is a lot  more brighter and stable than mCherry at 561nm.

Soren


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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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Hi everybody,



Has anybody tested TagRFP, and eventually compared it to mCherry?



It seems from the literature that TagRFP is much brighter. Is it also
photostable? On our scopes, we have filters matching both proteins, we
just want to pick up the "best" one...



Thanks in advance for your feedback!



Laurent.





___________________________

Laurent Gelman, PhD

Friedrich Miescher Institut

Facility for Advanced Imaging and Microscopy

Head of Light Microscopy

WRO 1066.2.16

Maulbeerstrasse 66

CH-4058 Basel

[phone]




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