Thorlabs 4 Color LED

[Christoph Heyder]

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Hello all,

I am a putting together a fluorescence microscope mainly for
deconvolution microscopy of fixed samples and have been looking at LED
light sources. Due to our limited budget, if am considering the 4 color
LED system from Thorlabs.

I am wondering if anyone is using it or has tested its intensity,
perhaps in comparison to other multi-color LED systems (e.g. Lumencor
Spectra X, COOLED or Xcite).

Any input is appreciated,

Chris

[Shalin Mehta]

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Hi Christopher,
I have not used Thorlabs LEDs. In case they are not good enough for you, an
option worth considering is Cairn's OptoLED:
http://www.cairn-research.co.uk/products/illumination/led-light-sources.
Their blue LED is certainly bright enough for typical sample with GFP .
Their electronics can transiently overdrive LED during the short exposure
time if your application demands. There are some other niceties in their
design. The 2 channel controller with 2 LEDs costs approx. USD 8K if I
recall correctly. But, there is a lite version which we haven't tried. If
you don't need all 4 channels at the same time, this may work for you.
James Kerin <[hidden email]> is our contact there.

Not commercial interest - just a satisfied user.

Shalin

website: http://mshalin.com
(office) Lillie 110, (ph) 508-289-7374.

HFSP Postdoctoral Fellow,
Marine Biological Laboratory,
7 MBL Street, Woods Hole MA 02543, USA


On Mar 1, 2013 5:11 AM, "Christoph Heyder" <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
> *****
>
> Hello all,
>
> I am a putting together a fluorescence microscope mainly for deconvolution
> microscopy of fixed samples and have been looking at LED light sources. Due
> to our limited budget, if am considering the 4 color LED system from
> Thorlabs.
>
> I am wondering if anyone is using it or has tested its intensity, perhaps
> in comparison to other multi-color LED systems (e.g. Lumencor Spectra X,
> COOLED or Xcite).
>
> Any input is appreciated,
>
> Chris
>

[Periasamy, Ammasi (ap3t)]

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I have heard good things about the Excite (EXFO) LED system
(http://www.ldgi-xcite.com/products-xled1-configurator.php). This company has been around for a while and products are good.    

No commercial interest

Dr. Ammasi Periasamy
Professor & Center Director
W.M. Keck Center for Cellular Imaging (KCCI)
(A University Imaging Center)
Biology, University of Virginia
Mail or FedEx: 485 McCormick Rd.
Charlottesville, VA 22904.
Office Location: Physical Life Sciences Building (B005)
90, Geldard Drive, Charlottesville, VA 22904
Voice: 434-243-7602 (Office); 982-4869 (lab)
Fax:[phone]; Email:[hidden email]
http://www.kcci.virginia.edu/contact/peri.php
************************
12th Annual Workshop on FRET Microscopy, March 11-16, 2013
http://www.kcci.virginia.edu/workshop/workshop2013/index.php
*************************

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Shalin Mehta
Sent: March-01-13 1:40 PM
To: [hidden email]
Subject: Re: Thorlabs 4 Color LED

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Hi Christopher,
I have not used Thorlabs LEDs. In case they are not good enough for you, an option worth considering is Cairn's OptoLED:
http://www.cairn-research.co.uk/products/illumination/led-light-sources.
Their blue LED is certainly bright enough for typical sample with GFP .
Their electronics can transiently overdrive LED during the short exposure time if your application demands. There are some other niceties in their design. The 2 channel controller with 2 LEDs costs approx. USD 8K if I recall correctly. But, there is a lite version which we haven't tried. If you don't need all 4 channels at the same time, this may work for you.
James Kerin <[hidden email]> is our contact there.

Not commercial interest - just a satisfied user.

Shalin

website: http://mshalin.com
(office) Lillie 110, (ph) 508-289-7374.

HFSP Postdoctoral Fellow,
Marine Biological Laboratory,
7 MBL Street, Woods Hole MA 02543, USA


On Mar 1, 2013 5:11 AM, "Christoph Heyder" < [hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.u
> mn.edu/cgi-bin/wa?A0=confocalmicroscopy>
> *****
>
> Hello all,
>
> I am a putting together a fluorescence microscope mainly for
> deconvolution microscopy of fixed samples and have been looking at LED
> light sources. Due to our limited budget, if am considering the 4
> color LED system from Thorlabs.
>
> I am wondering if anyone is using it or has tested its intensity,
> perhaps in comparison to other multi-color LED systems (e.g. Lumencor
> Spectra X, COOLED or Xcite).
>
> Any input is appreciated,
>
> Chris
>

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