Tissue phantom for MP

[Vladimir Ghukasyan-2]

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Dear Listers,

I'm thinking of a possible tissue phantom to test the penetration
depth of a multiphoton microscope.
I would be thankful if you have any ideas/experience.

Thank you in advance,
Vladimir

[Mollie Lange]

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Vladimir,
People who develop MRI sequences use tissue phantoms.  I think the phantoms are agar based. If you have an MRI group close by, you could check with them.
Best,
Mollie

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Vladimir Gukassyan
Sent: Friday, May 13, 2011 4:04 PM
To: [hidden email]
Subject: Tissue phantom for MP

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Dear Listers,

I'm thinking of a possible tissue phantom to test the penetration
depth of a multiphoton microscope.
I would be thankful if you have any ideas/experience.

Thank you in advance,
Vladimir



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[Kate Luby-Phelps]

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I have successfully used a mixture of low melting temperature agarose with
reconstituted powdered milk to make it turbid. The agarose concentration needs
to be high enough that the gel does not jiggle when it solidifies. I don't have my
notebook here so I can't tell you exactly what concentrations I used.

You can either include Tetraspeck beads as point sources of fluorescence or a
soluble fluorophore to get more uniform fluorescence. I put the mixture in a 35
mm dish and let it gel overnight before putting it on the scope. It is important to
keep it hydrated. The samples don't keep forever even in the refrigerator
because the milk spoils. So I make the samples fresh from stock solutions the
day before I use them. Here is a reference from Peter So's lab describing a
similar approach.


Journal of Biomedical Optics 8(3), 450–459 (July 2003)


Kate

[stu_the_flat]

Hello Vladimir

 

At present I am developing a method to mimic cardiac muscle. I am using a 1% agar mix and 100nm non-fluorescent beads to induce scattering.

 

To measure the light absorption and scattering through depth of tissue have dispersed live dissociated cardomyctes through the media and then look at the signal intensities as I image deeper in the media.

 

Hope that helps

 

Stuart

--- On Fri, 13/5/11, Vladimir Gukassyan [via Confocal Microscopy List] <[hidden email]> wrote:

From: Vladimir Gukassyan [via Confocal Microscopy List] <[hidden email]>
Subject: Tissue phantom for MP
To: "stu_the_flat" <[hidden email]>
Date: Friday, 13 May, 2011, 21:05

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Dear Listers,

I'm thinking of a possible tissue phantom to test the penetration
depth of a multiphoton microscope.
I would be thankful if you have any ideas/experience.

Thank you in advance,
Vladimir

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