ToPro3 vs DRAQ5
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I need to buy a nuclear stain equivalent to DAPI that fluoresces in the far red. This is for our core facility where potential users might work with mammalian tissues, plant, fungi and every other thing that has DNA in it. I want a stock that people can try out and determine whether it works for them. I would prefer only having one such dye and can't decide between TOPRO3 and DRAQ5. Any comments on which of these (or another alternative) is the most versatile? Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) [hidden email]<mailto:[hidden email]> http://www.biology.missouri.edu/faculty/phillips.html http://www.biotech.missouri.edu/mcc/ |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** We use both - but the nice thing about DRAQ5 is that it can be used to stain living cells as well as fixed ones. Of course, the cells won't divide in it, so you can't use it for long-term timelapse imaging, but it's still very useful for shorter timelapses. Best, Alison On 4/21/2011 3:51 PM, Phillips, Thomas E. wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > I need to buy a nuclear stain equivalent to DAPI that fluoresces in the far red. This is for our core facility where potential users might work with mammalian tissues, plant, fungi and every other thing that has DNA in it. I want a stock that people can try out and determine whether it works for them. I would prefer only having one such dye and can't decide between TOPRO3 and DRAQ5. Any comments on which of these (or another alternative) is the most versatile? > > > Thomas E. Phillips, Ph.D > Professor of Biological Sciences > Director, Molecular Cytology Core > 2 Tucker Hall > University of Missouri > Columbia, MO 65211-7400 > 573-882-4712 (office) > 573-882-0123 (fax) > [hidden email]<mailto:[hidden email]> > > http://www.biology.missouri.edu/faculty/phillips.html > http://www.biotech.missouri.edu/mcc/ -- Alison J. North, Ph.D., Research Associate Professor and Senior Director of the Bio-Imaging Resource Center, The Rockefeller University, 1230 York Avenue, New York, NY 10065. Tel: office ++ 212 327 7488 Tel: lab ++ 212 327 7486 Fax: ++ 212 327 7489 |
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In reply to this post by Phillips, Thomas E.
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi I have used TOPRO-3, it does photo bleach relatively rapidly and need to play with it more before I would say it's a good replacement for DAPI. Russ On 4/21/2011 2:51 PM, Phillips, Thomas E. wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > I need to buy a nuclear stain equivalent to DAPI that fluoresces in the far red. This is for our core facility where potential users might work with mammalian tissues, plant, fungi and every other thing that has DNA in it. I want a stock that people can try out and determine whether it works for them. I would prefer only having one such dye and can't decide between TOPRO3 and DRAQ5. Any comments on which of these (or another alternative) is the most versatile? > > > Thomas E. Phillips, Ph.D > Professor of Biological Sciences > Director, Molecular Cytology Core > 2 Tucker Hall > University of Missouri > Columbia, MO 65211-7400 > 573-882-4712 (office) > 573-882-0123 (fax) > [hidden email]<mailto:[hidden email]> > > http://www.biology.missouri.edu/faculty/phillips.html > http://www.biotech.missouri.edu/mcc/ |
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In reply to this post by Phillips, Thomas E.
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi, We have been very successful with DRAQ5 in both fixed and live cell imaging. It is quite resistant to photobleaching and doesn't need RNAse treatment to reduce RNA staining. Jake Cha > Date: Thu, 21 Apr 2011 14:51:23 -0500 > From: [hidden email] > Subject: ToPro3 vs DRAQ5 > To: [hidden email] > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > I need to buy a nuclear stain equivalent to DAPI that fluoresces in the far red. This is for our core facility where potential users might work with mammalian tissues, plant, fungi and every other thing that has DNA in it. I want a stock that people can try out and determine whether it works for them. I would prefer only having one such dye and can't decide between TOPRO3 and DRAQ5. Any comments on which of these (or another alternative) is the most versatile? > > > Thomas E. Phillips, Ph.D > Professor of Biological Sciences > Director, Molecular Cytology Core > 2 Tucker Hall > University of Missouri > Columbia, MO 65211-7400 > 573-882-4712 (office) > 573-882-0123 (fax) > [hidden email]<mailto:[hidden email]> > > http://www.biology.missouri.edu/faculty/phillips.html > http://www.biotech.missouri.edu/mcc/ |
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In reply to this post by Alison J. North
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** We have used 7-amino-actinomycin D (7-aad) with some success. Joel On Thu, Apr 21, 2011 at 4:07 PM, Alison North <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > We use both - but the nice thing about DRAQ5 is that it can be used to > stain living cells as well as fixed ones. Of course, the cells won't divide > in it, so you can't use it for long-term timelapse imaging, but it's still > very useful for shorter timelapses. > > Best, > Alison > > > On 4/21/2011 3:51 PM, Phillips, Thomas E. wrote: > >> ***** >> >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> I need to buy a nuclear stain equivalent to DAPI that fluoresces in the >> far red. This is for our core facility where potential users might work >> with mammalian tissues, plant, fungi and every other thing that has DNA in >> it. I want a stock that people can try out and determine whether it works >> for them. I would prefer only having one such dye and can't decide between >> TOPRO3 and DRAQ5. Any comments on which of these (or another alternative) >> is the most versatile? >> >> >> Thomas E. Phillips, Ph.D >> Professor of Biological Sciences >> Director, Molecular Cytology Core >> 2 Tucker Hall >> University of Missouri >> Columbia, MO 65211-7400 >> 573-882-4712 (office) >> 573-882-0123 (fax) >> [hidden email]<mailto:[hidden email]> >> >> http://www.biology.missouri.edu/faculty/phillips.html >> http://www.biotech.missouri.edu/mcc/ >> > > -- > Alison J. North, Ph.D., > Research Associate Professor and > Senior Director of the Bio-Imaging Resource Center, > The Rockefeller University, > 1230 York Avenue, > New York, > NY 10065. > Tel: office ++ 212 327 7488 > Tel: lab ++ 212 327 7486 > Fax: ++ 212 327 7489 > -- Joel B. Sheffield, Ph.D Department of Biology Temple University Philadelphia, PA 19122 Voice: 215 204 8839 e-mail: [hidden email] URL: http://astro.temple.edu/~jbs |
 
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Ignatius, Mike-2 |
Re: ToPro3 vs DRAQ5 Vendor Response
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi, Another suggestion from Molecular Probes: H10294, Nuclear Mask deep red stain. Very work flow friendly, as it stains nuclei of live or fixed cells. In addition it survives fixation and detergents. Emits in the far red with 633 excitation. Adherent cells won't divide much in it, though blood cells divide in it, and show no toxic effects. To-Pro 3 won't stain live cells, it is for fixed cells. Mike Ignatius Molecular Probes -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of JOEL B. SHEFFIELD Sent: Friday, April 22, 2011 4:39 AM To: [hidden email] Subject: Re: ToPro3 vs DRAQ5 ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** We have used 7-amino-actinomycin D (7-aad) with some success. Joel On Thu, Apr 21, 2011 at 4:07 PM, Alison North <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > We use both - but the nice thing about DRAQ5 is that it can be used to > stain living cells as well as fixed ones. Of course, the cells won't divide > in it, so you can't use it for long-term timelapse imaging, but it's still > very useful for shorter timelapses. > > Best, > Alison > > > On 4/21/2011 3:51 PM, Phillips, Thomas E. wrote: > >> ***** >> >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> I need to buy a nuclear stain equivalent to DAPI that fluoresces in the >> far red. This is for our core facility where potential users might work >> with mammalian tissues, plant, fungi and every other thing that has DNA in >> it. I want a stock that people can try out and determine whether it works >> for them. I would prefer only having one such dye and can't decide between >> TOPRO3 and DRAQ5. Any comments on which of these (or another alternative) >> is the most versatile? >> >> >> Thomas E. Phillips, Ph.D >> Professor of Biological Sciences >> Director, Molecular Cytology Core >> 2 Tucker Hall >> University of Missouri >> Columbia, MO 65211-7400 >> 573-882-4712 (office) >> 573-882-0123 (fax) >> [hidden email]<mailto:[hidden email]> >> >> http://www.biology.missouri.edu/faculty/phillips.html >> http://www.biotech.missouri.edu/mcc/ >> > > -- > Alison J. North, Ph.D., > Research Associate Professor and > Senior Director of the Bio-Imaging Resource Center, > The Rockefeller University, > 1230 York Avenue, > New York, > NY 10065. > Tel: office ++ 212 327 7488 > Tel: lab ++ 212 327 7486 > Fax: ++ 212 327 7489 > -- Joel B. Sheffield, Ph.D Department of Biology Temple University Philadelphia, PA 19122 Voice: 215 204 8839 e-mail: [hidden email] URL: http://astro.temple.edu/~jbs |
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Phillips, Thomas E. |
Re: ToPro3 vs DRAQ5 Vendor Response
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Mike - Thanks for the alternative suggestion - I looked at your website and this stain was clearly effective used in some nuclear segmentation studies. But it is fair to say that it has higher cytoplasmic staining that ToPro3? The images suggested that. I realize its advantage is it works on both live and fixed cells. One real concern I have on ToPro3 is that it is sold as a DMSO stock. This requires a tad higher degree of care when you are talking about a DNA intercalating agent in this solvent. I believe the DRAQ5 stock is aqueous - still undoubtedly toxic but presumably latex gloves would be protective. Comment? Thanks for your insight. I am a big fan of both the Molecular Probes family of labels and their incredible website with so much info. Tom Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) [hidden email] http://www.biology.missouri.edu/faculty/phillips.html http://www.biotech.missouri.edu/mcc/ -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ignatius, Mike Sent: Friday, April 22, 2011 2:44 PM To: [hidden email] Subject: Re: ToPro3 vs DRAQ5 Vendor Response ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi, Another suggestion from Molecular Probes: H10294, Nuclear Mask deep red stain. Very work flow friendly, as it stains nuclei of live or fixed cells. In addition it survives fixation and detergents. Emits in the far red with 633 excitation. Adherent cells won't divide much in it, though blood cells divide in it, and show no toxic effects. To-Pro 3 won't stain live cells, it is for fixed cells. Mike Ignatius Molecular Probes -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of JOEL B. SHEFFIELD Sent: Friday, April 22, 2011 4:39 AM To: [hidden email] Subject: Re: ToPro3 vs DRAQ5 ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** We have used 7-amino-actinomycin D (7-aad) with some success. Joel On Thu, Apr 21, 2011 at 4:07 PM, Alison North <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > We use both - but the nice thing about DRAQ5 is that it can be used to > stain living cells as well as fixed ones. Of course, the cells won't divide > in it, so you can't use it for long-term timelapse imaging, but it's still > very useful for shorter timelapses. > > Best, > Alison > > > On 4/21/2011 3:51 PM, Phillips, Thomas E. wrote: > >> ***** >> >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> I need to buy a nuclear stain equivalent to DAPI that fluoresces in the >> far red. This is for our core facility where potential users might work >> with mammalian tissues, plant, fungi and every other thing that has DNA in >> it. I want a stock that people can try out and determine whether it works >> for them. I would prefer only having one such dye and can't decide between >> TOPRO3 and DRAQ5. Any comments on which of these (or another alternative) >> is the most versatile? >> >> >> Thomas E. Phillips, Ph.D >> Professor of Biological Sciences >> Director, Molecular Cytology Core >> 2 Tucker Hall >> University of Missouri >> Columbia, MO 65211-7400 >> 573-882-4712 (office) >> 573-882-0123 (fax) >> [hidden email]<mailto:[hidden email]> >> >> http://www.biology.missouri.edu/faculty/phillips.html >> http://www.biotech.missouri.edu/mcc/ >> > > -- > Alison J. North, Ph.D., > Research Associate Professor and > Senior Director of the Bio-Imaging Resource Center, > The Rockefeller University, > 1230 York Avenue, > New York, > NY 10065. > Tel: office ++ 212 327 7488 > Tel: lab ++ 212 327 7486 > Fax: ++ 212 327 7489 > -- Joel B. Sheffield, Ph.D Department of Biology Temple University Philadelphia, PA 19122 Voice: 215 204 8839 e-mail: [hidden email] URL: http://astro.temple.edu/~jbs |
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Amanda M. Lawrence |
Microscopy and Microanalysis 2011 in Nashville, TN
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Greetings, To all students (or those of you who might have students) attending the 2011 M&M meeting in Nashville, TN, please consider the student bursary program offered by MSA. The purpose of these bursaries is to encourage students to attend the annual MSA/MAS Microscopy and Microanalysis meeting, where they can meet and interact with the established microscopy community while defraying some meeting costs. Students work for 20 hours (or up to 40 hours) during the meeting and pre-meeting events and are paid $10 an hour. The jobs involve such things as providing support in the different symposia (helping with audio-visual needs, maintaining an attendance count, and helping speakers set up for their presentation), staffing the volunteer office, monitoring use of the Internet Café, and helping with vendor tutorials and poster set-up. Payment is given as a check at the end of the meetings or when the student leaves Nashville. Once the task list has been finalized, each bursary will be contacted and allowed to choose the times and activities they would like to work. Many times they end up *working* sessions they would attend anyway. There is an added bonus of $10 cash meal allotment for each morning and/or afternoon worked and a meeting shirt. If anyone would like to participate in the bursary program, please check the *I wish to apply for a student bursary* box in section 2 of the registration form. Bursary space is limited, so sign-up early. Applicants for the bursaries must be members of MSA or MAS, enrolled as students at a recognized educational institution, and have paid the registration fee. For those *non-students* volunteers are needed to help with the above mentioned meeting activities as well. Although not paid on an hourly basis as the student bursaries, volunteers do receive the same cash allotment for meals and shirt. Plus they also have the opportunity to interact more with the microscopy community as they assist with meeting tasks. If anyone has any questions about the bursary/volunteer program, or would like to participate, please contact: Amanda Lawrence Institute for Imaging and Analytical Technologies Mississippi State University 662-325-3019 [hidden email] |
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Ignatius, Mike-2 |
Re: ToPro3 vs DRAQ5 Vendor Response
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In reply to this post by Phillips, Thomas E.
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Tom, Yes, like Draq5, HCS Nuclear Mask Deep Red does have some cytoplasmic staining, presumably RNA. For image segmentation, in HCS mode, this is a positive feature for many users. It allows one to segment on either the nuclear or cytoplasmic compartment. If you want just nuclear, reducing the concentration and thresholding for higher signals can help. Yes, Topro3 is a more nuclear specific stain, but again only works on dead cells. And can't argue with that the DMSO will hasten delivery, but aqueous material can get into skin as well. We only use DMSO when it is completely warranted for solubility. PPE (esp gloves) is always encouraged with any dye - esp DNA stains. Though Ethidium Bromide, was used as meat coloring and fed to cattle in the past, so we may have some capacity to tolerate it. Google ethidium and food dyes for interesting commentary there. Thanks for the positive words - the site does make a sincere effort trying to get the science out in front of anything we do. Hopefully you get our BioProbes issues 2-3 times a year - to keep even more current on new apps and materials. Best, Mike -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Phillips, Thomas E. Sent: Sunday, April 24, 2011 9:46 AM To: [hidden email] Subject: Re: ToPro3 vs DRAQ5 Vendor Response ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Mike - Thanks for the alternative suggestion - I looked at your website and this stain was clearly effective used in some nuclear segmentation studies. But it is fair to say that it has higher cytoplasmic staining that ToPro3? The images suggested that. I realize its advantage is it works on both live and fixed cells. One real concern I have on ToPro3 is that it is sold as a DMSO stock. This requires a tad higher degree of care when you are talking about a DNA intercalating agent in this solvent. I believe the DRAQ5 stock is aqueous - still undoubtedly toxic but presumably latex gloves would be protective. Comment? Thanks for your insight. I am a big fan of both the Molecular Probes family of labels and their incredible website with so much info. Tom Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) [hidden email] http://www.biology.missouri.edu/faculty/phillips.html http://www.biotech.missouri.edu/mcc/ -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ignatius, Mike Sent: Friday, April 22, 2011 2:44 PM To: [hidden email] Subject: Re: ToPro3 vs DRAQ5 Vendor Response ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi, Another suggestion from Molecular Probes: H10294, Nuclear Mask deep red stain. Very work flow friendly, as it stains nuclei of live or fixed cells. In addition it survives fixation and detergents. Emits in the far red with 633 excitation. Adherent cells won't divide much in it, though blood cells divide in it, and show no toxic effects. To-Pro 3 won't stain live cells, it is for fixed cells. Mike Ignatius Molecular Probes -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of JOEL B. SHEFFIELD Sent: Friday, April 22, 2011 4:39 AM To: [hidden email] Subject: Re: ToPro3 vs DRAQ5 ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** We have used 7-amino-actinomycin D (7-aad) with some success. Joel On Thu, Apr 21, 2011 at 4:07 PM, Alison North <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > We use both - but the nice thing about DRAQ5 is that it can be used to > stain living cells as well as fixed ones. Of course, the cells won't divide > in it, so you can't use it for long-term timelapse imaging, but it's still > very useful for shorter timelapses. > > Best, > Alison > > > On 4/21/2011 3:51 PM, Phillips, Thomas E. wrote: > >> ***** >> >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> I need to buy a nuclear stain equivalent to DAPI that fluoresces in the >> far red. This is for our core facility where potential users might work >> with mammalian tissues, plant, fungi and every other thing that has DNA in >> it. I want a stock that people can try out and determine whether it works >> for them. I would prefer only having one such dye and can't decide between >> TOPRO3 and DRAQ5. Any comments on which of these (or another alternative) >> is the most versatile? >> >> >> Thomas E. Phillips, Ph.D >> Professor of Biological Sciences >> Director, Molecular Cytology Core >> 2 Tucker Hall >> University of Missouri >> Columbia, MO 65211-7400 >> 573-882-4712 (office) >> 573-882-0123 (fax) >> [hidden email]<mailto:[hidden email]> >> >> http://www.biology.missouri.edu/faculty/phillips.html >> http://www.biotech.missouri.edu/mcc/ >> > > -- > Alison J. North, Ph.D., > Research Associate Professor and > Senior Director of the Bio-Imaging Resource Center, > The Rockefeller University, > 1230 York Avenue, > New York, > NY 10065. > Tel: office ++ 212 327 7488 > Tel: lab ++ 212 327 7486 > Fax: ++ 212 327 7489 > -- Joel B. Sheffield, Ph.D Department of Biology Temple University Philadelphia, PA 19122 Voice: 215 204 8839 e-mail: [hidden email] URL: http://astro.temple.edu/~jbs |
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In reply to this post by Alison J. North
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** WE did a few trials with DRAQ5 in the past and found that it gets brighter when illuminated, which created some problems for us, also we observed in preliminary tests that it stops RelA translocation in A549 cells after TNFalfa stimulation (1-2 hrs incubations), so we dropped it -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Alison North Sent: Thursday, April 21, 2011 3:08 PM To: [hidden email] Subject: Re: ToPro3 vs DRAQ5 ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** We use both - but the nice thing about DRAQ5 is that it can be used to stain living cells as well as fixed ones. Of course, the cells won't divide in it, so you can't use it for long-term timelapse imaging, but it's still very useful for shorter timelapses. Best, Alison On 4/21/2011 3:51 PM, Phillips, Thomas E. wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > I need to buy a nuclear stain equivalent to DAPI that fluoresces in the far red. This is for our core facility where potential users might work with mammalian tissues, plant, fungi and every other thing that has DNA in it. I want a stock that people can try out and determine whether it works for them. I would prefer only having one such dye and can't decide between TOPRO3 and DRAQ5. Any comments on which of these (or another alternative) is the most versatile? > > > Thomas E. Phillips, Ph.D > Professor of Biological Sciences > Director, Molecular Cytology Core > 2 Tucker Hall > University of Missouri > Columbia, MO 65211-7400 > 573-882-4712 (office) > 573-882-0123 (fax) > [hidden email]<mailto:[hidden email]> > > http://www.biology.missouri.edu/faculty/phillips.html > http://www.biotech.missouri.edu/mcc/ -- Alison J. North, Ph.D., Research Associate Professor and Senior Director of the Bio-Imaging Resource Center, The Rockefeller University, 1230 York Avenue, New York, NY 10065. Tel: office ++ 212 327 7488 Tel: lab ++ 212 327 7486 Fax: ++ 212 327 7489 |
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