Tokai Hit vs OkoLabs stage top incubation for Zeiss LMS 880?

[Mary Ellen Pease]

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HI all,

We have a Zeiss LSM 880 NLO on the AxioExaminer/upright body with 35 channel GaAsP spectral detector, BiG detector, Airyscan and Airyscan Fast. We have both the Prior Z deck, and the Piezo Z stages, though we found out that switching between the two is more difficult/time consuming than we first realized. Currently the Piezo Z stage is in place.

We wish to purchase an incubation system for use with our dipping lenses and it appears that Tokai Hit and OkoLabs are the main systems out there. I tried to get a Tokai Hit demo but the unit is booked until April, 2020. I reached out to and got a quote for a similar unit from OkoLabs but now we are ready to purchase and I'm concerned about buying either units sight unseen or without vetting from people in the community. What are people's experience with these systems? Thanks in advance.

Best,
Mary Ellen Pease
Manager, Wilmer Microscopy and Imaging Core Facility
Johns Hopkins University School of Medicine
[hidden email]

[Romin, Yevgeniy/Sloan Kettering Institute]

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Hi Mary

We don’t have okolabs in the facility here, but we have several Tokai hit incubation Systems that we use on our Leica Confocals. I have nothing but good things to say about them. On our Zeiss LSM 880 we use the zeiss incubation system, seems to be working all right.

Yevgeniy

> On Dec 9, 2019, at 18:08, Mary Ellen Pease <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> HI all,
>
> We have a Zeiss LSM 880 NLO on the AxioExaminer/upright body with 35 channel GaAsP spectral detector, BiG detector, Airyscan and Airyscan Fast. We have both the Prior Z deck, and the Piezo Z stages, though we found out that switching between the two is more difficult/time consuming than we first realized. Currently the Piezo Z stage is in place.
>
> We wish to purchase an incubation system for use with our dipping lenses and it appears that Tokai Hit and OkoLabs are the main systems out there. I tried to get a Tokai Hit demo but the unit is booked until April, 2020. I reached out to and got a quote for a similar unit from OkoLabs but now we are ready to purchase and I'm concerned about buying either units sight unseen or without vetting from people in the community. What are people's experience with these systems? Thanks in advance.
>
> Best,
> Mary Ellen Pease
> Manager, Wilmer Microscopy and Imaging Core Facility
> Johns Hopkins University School of Medicine
> [hidden email]
>
>
>
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[Sylvie Le Guyader]

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Hi Mary



We have many Life Imaging Services from Switzerland. Seriously crap website<https://www.lis.ch/thebox.html> but we are very happy with their full size incubators. We have been using them for 10 years with very little troubleshooting and we have imaged sensitive things like early mouse embryos.



Med vänlig hälsning / Best regards



Sylvie



@@@@@@@@@@@@@@@@@@@@@@@@

Sylvie Le Guyader, PhD

Live Cell Imaging Facility Manager

Karolinska Institutet- Bionut Dpt

Blickagången 16,

Room 7362 (lab)/7840 (office)

14157 Huddinge, Sweden

mobile: [phone]

LCI website

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-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Mary Ellen Pease
Sent: 09 December 2019 23:19
To: [hidden email]
Subject: Tokai Hit vs OkoLabs stage top incubation for Zeiss LMS 880?



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HI all,



We have a Zeiss LSM 880 NLO on the AxioExaminer/upright body with 35 channel GaAsP spectral detector, BiG detector, Airyscan and Airyscan Fast. We have both the Prior Z deck, and the Piezo Z stages, though we found out that switching between the two is more difficult/time consuming than we first realized. Currently the Piezo Z stage is in place.



We wish to purchase an incubation system for use with our dipping lenses and it appears that Tokai Hit and OkoLabs are the main systems out there. I tried to get a Tokai Hit demo but the unit is booked until April, 2020. I reached out to and got a quote for a similar unit from OkoLabs but now we are ready to purchase and I'm concerned about buying either units sight unseen or without vetting from people in the community. What are people's experience with these systems? Thanks in advance.



Best,

Mary Ellen Pease

Manager, Wilmer Microscopy and Imaging Core Facility Johns Hopkins University School of Medicine [hidden email]<mailto:[hidden email]>



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[darren.thomson]

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Haven't used Tokai, but we are very happy with our Okolab stage top enclosure for CO2. The quality is very high and the magnetic inserts are nice. There was even an adapter for our Cellasic microfluidic plates, which was a bonus.

Let us know how you get on,

BW,

Dr Darren Thomson
Uo Manchester, UK.
[hidden email]

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Mary Ellen Pease
Sent: 09 December 2019 22:19
To: [hidden email]
Subject: Tokai Hit vs OkoLabs stage top incubation for Zeiss LMS 880?

*****
To join, leave or search the confocal microscopy listserv, go to:
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HI all,

We have a Zeiss LSM 880 NLO on the AxioExaminer/upright body with 35 channel GaAsP spectral detector, BiG detector, Airyscan and Airyscan Fast. We have both the Prior Z deck, and the Piezo Z stages, though we found out that switching between the two is more difficult/time consuming than we first realized. Currently the Piezo Z stage is in place.

We wish to purchase an incubation system for use with our dipping lenses and it appears that Tokai Hit and OkoLabs are the main systems out there. I tried to get a Tokai Hit demo but the unit is booked until April, 2020. I reached out to and got a quote for a similar unit from OkoLabs but now we are ready to purchase and I'm concerned about buying either units sight unseen or without vetting from people in the community. What are people's experience with these systems? Thanks in advance.

Best,
Mary Ellen Pease
Manager, Wilmer Microscopy and Imaging Core Facility
Johns Hopkins University School of Medicine
[hidden email]

[Dr. K N Ganesh]

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One our Olympus FV3000 upright confocal system with fixed stage has Tokai dedicated Incubator for upright scope with variable collar for various water dipping objectives and till now no complaints.
Reg
Ganesh

Sent from my iPhone

> On 10-Dec-2019, at 04:38, Mary Ellen Pease <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> HI all,
>
> We have a Zeiss LSM 880 NLO on the AxioExaminer/upright body with 35 channel GaAsP spectral detector, BiG detector, Airyscan and Airyscan Fast. We have both the Prior Z deck, and the Piezo Z stages, though we found out that switching between the two is more difficult/time consuming than we first realized. Currently the Piezo Z stage is in place.
>
> We wish to purchase an incubation system for use with our dipping lenses and it appears that Tokai Hit and OkoLabs are the main systems out there. I tried to get a Tokai Hit demo but the unit is booked until April, 2020. I reached out to and got a quote for a similar unit from OkoLabs but now we are ready to purchase and I'm concerned about buying either units sight unseen or without vetting from people in the community. What are people's experience with these systems? Thanks in advance.
>
> Best,
> Mary Ellen Pease
> Manager, Wilmer Microscopy and Imaging Core Facility
> Johns Hopkins University School of Medicine
> [hidden email]

[Dan Focht]

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Mary & "All"

There is another option that is an ideal companion for dipping lenses that provides more functionality than a heated box.
The Bioptechs Delta T system uses an ITO coated glass bottomed dish that generates heat directly to the specimen without having to heat a box or the stage. Temperature is established and held steady within two minutes with a proprietary thermal control system that eliminates the wait time and equilibration time of warming a box. Although originally designed for inverted use the following accessories make it an ideal companion for dipping lenses. It has the option of an intermediate piece to facilitate perfusion and humidified CO2 if needed. There is also an atmospheric barrier ring that surrounds the objective to exclude ambient atmosphere from the specimen area. The barrier ring fits around the objective and slides on top of the dish, or intermediate adapter, for X,Y  translation and allows the dipping lens to come into focus on the specimen. It accommodates adherent cells, live tissue and artificial membranes. It can be used on both upright and inverted scopes with high N.A. lenses. Take a look at it and the many accessories that accommodate a variety of specialized imaging scenarios.


Additional info can be found at the following links:
Make sure you scroll down the page to see details.

https://bioptechs.com/product/delta-t-starter-set/ <https://bioptechs.com/product/delta-t-starter-set/>

https://bioptechs.com/product/acbr/ <https://bioptechs.com/product/acbr/>



Dan


Dan Focht
Bioptechs, Inc.
3560 Beck Rd.
Butler PA 16002
V724-282-7145
F724-282-0745
Toll Free 877 lIVE-CELL (548-3235)
[hidden email]

> On Dec 9, 2019, at 5:19 PM, Mary Ellen Pease <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> HI all,
>
> We have a Zeiss LSM 880 NLO on the AxioExaminer/upright body with 35 channel GaAsP spectral detector, BiG detector, Airyscan and Airyscan Fast. We have both the Prior Z deck, and the Piezo Z stages, though we found out that switching between the two is more difficult/time consuming than we first realized. Currently the Piezo Z stage is in place.
>
> We wish to purchase an incubation system for use with our dipping lenses and it appears that Tokai Hit and OkoLabs are the main systems out there. I tried to get a Tokai Hit demo but the unit is booked until April, 2020. I reached out to and got a quote for a similar unit from OkoLabs but now we are ready to purchase and I'm concerned about buying either units sight unseen or without vetting from people in the community. What are people's experience with these systems? Thanks in advance.
>
> Best,
> Mary Ellen Pease
> Manager, Wilmer Microscopy and Imaging Core Facility
> Johns Hopkins University School of Medicine
> [hidden email]

[weber]

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Hi all,

I did few years ago my own thermal box. As i remember the cost was less than 1000€.
It was on LSM 7MP .
I can send images about it i f you want.

Let me know.

Regards