Tool for Tissue Sectioning-- 100 to 700um Slices--

[Paulos Mengsteab]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi,

I am trying to sectioning mouse and rat knee joints. I would like to consistently section the
tissue in the range of 100um to 2mm in increments of 100um. However, I can not find a
cost-effective tool to do this.

Does anyone have a suggestion on a sectioning instrument for my application?

Thanks in advance for your help!

Best,
Paulos Mengsteab

[Patrick Van Oostveldt]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear,

Just from my gut feeling I should consider a vibratome. There are a lot of references on google. Leica has some sofisticated instruments, but other companies also have good working model.

Patrick Van Oostveldt
Sint-Denijslaan 199
9000 GENT

Mobile [phone]
Sent from my iPad

> On 11 Mar 2016, at 15:23, Paulos Mengsteab <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi,
>
> I am trying to sectioning mouse and rat knee joints. I would like to consistently section the
> tissue in the range of 100um to 2mm in increments of 100um. However, I can not find a
> cost-effective tool to do this.
>
> Does anyone have a suggestion on a sectioning instrument for my application?
>
> Thanks in advance for your help!
>
> Best,
> Paulos Mengsteab

[Michelle Aloni]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Paulos,

You know that you will have to fix and decalcify your specimens first for any kind of sectioning (this size) right?

Michelle Aloni
USC School of Mediscine
Los Angeles, CA
________________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of patrick van oostveldt <[hidden email]>
Sent: Saturday, March 12, 2016 5:54 AM
To: [hidden email]
Subject: Re: Tool for Tissue Sectioning-- 100 to 700um Slices--

*****
To join, leave or search the confocal microscopy listserv, go to:
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=CwIFAg&c=clK7kQUTWtAVEOVIgvi0NU5BOUHhpN0H8p7CSfnc_gI&r=vgF50MHOKVckLpjE2pt3zg&m=lx4_6diaaoe-ofLTWBiP29TPUnrBMQvwndTKAL_uPRE&s=QbTjtWQGJGS9baAVBzAXFtx0gfXXs0Xe_0HOJg-5FNc&e=
Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=CwIFAg&c=clK7kQUTWtAVEOVIgvi0NU5BOUHhpN0H8p7CSfnc_gI&r=vgF50MHOKVckLpjE2pt3zg&m=lx4_6diaaoe-ofLTWBiP29TPUnrBMQvwndTKAL_uPRE&s=mztQFIHoJNgBSzyOpUZPsOhsgds6kLug82f1fCg11Js&e=  and include the link in your posting.
*****

Dear,

Just from my gut feeling I should consider a vibratome. There are a lot of references on google. Leica has some sofisticated instruments, but other companies also have good working model.

Patrick Van Oostveldt
Sint-Denijslaan 199
9000 GENT

Mobile [phone]
Sent from my iPad

> On 11 Mar 2016, at 15:23, Paulos Mengsteab <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=CwIFAg&c=clK7kQUTWtAVEOVIgvi0NU5BOUHhpN0H8p7CSfnc_gI&r=vgF50MHOKVckLpjE2pt3zg&m=lx4_6diaaoe-ofLTWBiP29TPUnrBMQvwndTKAL_uPRE&s=QbTjtWQGJGS9baAVBzAXFtx0gfXXs0Xe_0HOJg-5FNc&e=
> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=CwIFAg&c=clK7kQUTWtAVEOVIgvi0NU5BOUHhpN0H8p7CSfnc_gI&r=vgF50MHOKVckLpjE2pt3zg&m=lx4_6diaaoe-ofLTWBiP29TPUnrBMQvwndTKAL_uPRE&s=mztQFIHoJNgBSzyOpUZPsOhsgds6kLug82f1fCg11Js&e=  and include the link in your posting.
> *****
>
> Hi,
>
> I am trying to sectioning mouse and rat knee joints. I would like to consistently section the
> tissue in the range of 100um to 2mm in increments of 100um. However, I can not find a
> cost-effective tool to do this.
>
> Does anyone have a suggestion on a sectioning instrument for my application?
>
> Thanks in advance for your help!
>
> Best,
> Paulos Mengsteab

[Daniel Possin]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

You will need to decalcify your specimens and use a good vibratome with a sapphire knife (Delaware Diamond Knives). Steel knives will not work. Cut with moderately high vibration amplitude and a very slow advance rate (<1.0 mm/sec).

Dan

Sent from my iPhone

> On Mar 11, 2016, at 6:23 AM, Paulos Mengsteab <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi,
>
> I am trying to sectioning mouse and rat knee joints. I would like to consistently section the
> tissue in the range of 100um to 2mm in increments of 100um. However, I can not find a
> cost-effective tool to do this.
>
> Does anyone have a suggestion on a sectioning instrument for my application?
>
> Thanks in advance for your help!
>
> Best,
> Paulos Mengsteab