Used confocal?

[Jonathan M Krupp]

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Greetings

I am not a real confocal guy, but I monitor this list. In a past life I looked after an instrument, but no  more.

I am now an instructor at Delta College in Stockton, California where we have one of only a few EM training programs at the community college level.

I want to jazz up the light microscopy side of things here. We have several light microscopes with cameras and I want to add something like confocal.

We have a widefield fluorescence scope that can introduce  simple fluorescence, but I tell them all about confocal (at least as much as I know).

They need some hands on experience so they can really 'get it'.

Funding is the same sad song, there is none, or at least none unless a great deal shows up.

Anybody have advice or a spare scope to share?

Thanks

Jon

[George McNamara]

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Hi Jon,

I still have a Zeiss LSM510 (4-PMT, not META), that I have permission to
sell off (not give away). No longer under service contract. Argon ion
laser is a year old and I expect it to die soon (still has HeNe 543 and
HeNe 633 nm lasers). Zeiss can likely round up Zeiss crates for shipping
- buyer would be responsible for all shipping costs, Zeiss
pre-inspection/packing/unpacking/installing/calibrating (shipping and
Zeiss could be $10K, maybe more). For you, would make most sense to get
our Axiovert 200M as well - for others in needs of spare components I am
happy to hang on to the Axiovert (plan to turn it into a
MetaMorph/ORCA-ER acquisition system when the Argon laser and/or
scanhead die). Please contact me at www.sylvester.org/AICF if interested.


As for your class, consider one-upping the $3K single molecule WF+ TIRF
system (including 100x TIRF lens) described by

Demonstration of a low-cost, single molecule capable, multimode optical
microscope”
V. Protasenko, K. L. Hull, _M. Kuno_, Chem. Ed. *2005*, 10, 269.
DOI: 10.1333/s00897050912a <http://dx.doi.org/10.1333/s00897050912a>
http://nd.edu/~mkuno/Publications.html

I forget if it has a motorized stage ... if not, focusing the laser to a
point, adding a pinhole to the detector, and (maybe) Lego XY motors,
could get confocal ... some www.wickedlasers.com laser pointers might be
intense enough for multiphoton excitation.

Sincerely,

George


On 11/3/2011 4:49 PM, Jon Krupp wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Greetings
>
> I am not a real confocal guy, but I monitor this list. In a past life I looked after an instrument, but no  more.
>
> I am now an instructor at Delta College in Stockton, California where we have one of only a few EM training programs at the community college level.
>
> I want to jazz up the light microscopy side of things here. We have several light microscopes with cameras and I want to add something like confocal.
>
> We have a widefield fluorescence scope that can introduce  simple fluorescence, but I tell them all about confocal (at least as much as I know).
>
> They need some hands on experience so they can really 'get it'.
>
> Funding is the same sad song, there is none, or at least none unless a great deal shows up.
>
> Anybody have advice or a spare scope to share?
>
> Thanks
>
> Jon
>
>    

--


George McNamara, PhD
Analytical Imaging Core Facility
University of Miami

[Nobby Clark]

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To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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Do You have a budget? I can get you older cheaper units Leiva so on
Thanks
On Nov 3, 2011 2:25 PM, "Jon Krupp" <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Greetings
>
> I am not a real confocal guy, but I monitor this list. In a past life I
> looked after an instrument, but no  more.
>
> I am now an instructor at Delta College in Stockton, California where we
> have one of only a few EM training programs at the community college level.
>
> I want to jazz up the light microscopy side of things here. We have
> several light microscopes with cameras and I want to add something like
> confocal.
>
> We have a widefield fluorescence scope that can introduce  simple
> fluorescence, but I tell them all about confocal (at least as much as I
> know).
>
> They need some hands on experience so they can really 'get it'.
>
> Funding is the same sad song, there is none, or at least none unless a
> great deal shows up.
>
> Anybody have advice or a spare scope to share?
>
> Thanks
>
> Jon
>