Ekaterina PAPUSHEVA-2 |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear all, we have confocal stacks from several tissue sections, that are slightly rotated relative to each other. There are no overlapping landmarks, except matching tissue boundaries. We would like to stitch them in a single volume, after correcting the drift does someone know a good software, that can help with such semi/manual stitching? best regards, Ekaterina |
Armstrong, Brian |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi, I would use Amira for this. You can set landmarks based on structures and adjust the data sets until you are satisfied. http://www.vsg3d.com/amira/overview If I remember correctly, similar functionality exists in Velocity and Imaris. Cheers, Brian D Armstrong PhD Associate Research Professor Director, Light Microscopy Core Beckman Research Institute City of Hope Dept of Neuroscience 1450 E Duarte Rd Duarte, CA 91010 626-256-4673 x62872 -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ekaterina PAPUSHEVA Sent: Friday, July 19, 2013 1:02 AM To: [hidden email] Subject: Volume stitching from phzsical tissue sections? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear all, we have confocal stacks from several tissue sections, that are slightly rotated relative to each other. There are no overlapping landmarks, except matching tissue boundaries. We would like to stitch them in a single volume, after correcting the drift does someone know a good software, that can help with such semi/manual stitching? best regards, Ekaterina --------------------------------------------------------------------- *SECURITY/CONFIDENTIALITY WARNING: This message and any attachments are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. (fpc5p) --------------------------------------------------------------------- |
Niko Ehrenfeuchter |
In reply to this post by Ekaterina PAPUSHEVA-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear Ekaterina, On 19.07.2013 10:01, Ekaterina PAPUSHEVA wrote: > Dear all, > > we have confocal stacks from several tissue sections, that are > slightly rotated relative to each other. There are no overlapping > landmarks, except matching tissue boundaries. We would like to stitch > them in a single volume, after correcting the drift > > does someone know a good software, that can help with such > semi/manual stitching? that's *the* application for TrakEM2/Fiji. See the Fiji-Wiki for details, especially the video tutorials: http://fiji.sc/wiki/index.php/TrakEM2 http://fiji.sc/wiki/index.php/TrakEM2_tutorials Cheers Niko |
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