Volume stitching from phzsical tissue sections?

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Ekaterina PAPUSHEVA-2 Ekaterina PAPUSHEVA-2
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Volume stitching from phzsical tissue sections?

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Dear all,

we have confocal stacks from several tissue sections, that are slightly rotated relative to each other. There are no overlapping landmarks, except matching tissue boundaries. We would like to stitch them in a single volume, after correcting the drift

does someone know a good software, that can help with such semi/manual stitching?

best regards,
Ekaterina
Armstrong, Brian Armstrong, Brian
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Re: Volume stitching from phzsical tissue sections?

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Hi, I would use Amira for this. You can set landmarks based on structures and adjust the data sets until you are satisfied.
http://www.vsg3d.com/amira/overview

If I remember correctly,  similar functionality exists in Velocity and Imaris.

Cheers,

Brian D Armstrong PhD
Associate Research Professor
Director, Light Microscopy Core
Beckman Research Institute
City of Hope
Dept of Neuroscience
1450 E Duarte Rd
Duarte, CA 91010
626-256-4673 x62872


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ekaterina PAPUSHEVA
Sent: Friday, July 19, 2013 1:02 AM
To: [hidden email]
Subject: Volume stitching from phzsical tissue sections?

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Dear all,

we have confocal stacks from several tissue sections, that are slightly rotated relative to each other. There are no overlapping landmarks, except matching tissue boundaries. We would like to stitch them in a single volume, after correcting the drift

does someone know a good software, that can help with such semi/manual stitching?

best regards,
Ekaterina


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Niko Ehrenfeuchter Niko Ehrenfeuchter
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Re: Volume stitching from phzsical tissue sections?

In reply to this post by Ekaterina PAPUSHEVA-2
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Dear Ekaterina,

On 19.07.2013 10:01, Ekaterina PAPUSHEVA wrote:
> Dear all,
>
> we have confocal stacks from several tissue sections, that are
> slightly rotated relative to each other. There are no overlapping
> landmarks, except matching tissue boundaries. We would like to stitch
> them in a single volume, after correcting the drift
>
> does someone know a good software, that can help with such
> semi/manual stitching?

that's *the* application for TrakEM2/Fiji. See the Fiji-Wiki for
details, especially the video tutorials:

http://fiji.sc/wiki/index.php/TrakEM2
http://fiji.sc/wiki/index.php/TrakEM2_tutorials

Cheers
Niko