Water dispenser

[Damien Alcor]

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Hi,

I have a confocal Nikon A1R with the JOB module for multiwell plate
screening. Nikon does not provide a system to feed water (or oil for oil
objective) to maintain the contact using a water immersion objective
(40x WI). Would you know a company that could provide such a device?
Have you developped your own device?

Kind regards

Damien Alcor

--
Damien Alcor
Responsable Plateforme Biophotonique
Bâtiment Universitaire Archimed
INSERM U1065, C3M
151 route Saint Antoine de Ginestière
BP 2 3194
06204 NICE Cedex 3
Phone: [phone]
email:[hidden email]
http://www.unice.fr/c3m/index.php/the-facility/

[Kurt Thorn]

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On 4/4/2016 7:57 AM, Damien Alcor wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi,
>
> I have a confocal Nikon A1R with the JOB module for multiwell plate
> screening. Nikon does not provide a system to feed water (or oil for
> oil objective) to maintain the contact using a water immersion
> objective (40x WI). Would you know a company that could provide such a
> device? Have you developped your own device?
>
> Kind regards
>
> Damien Alcor
>

Sutter Instruments (http://sutter.com/) can machine a collar with a
needle for water dispensing, that you can then mate to a syringe pump.  
We got partway into setting up such a system for a 40x WI objective, but
the project that needed it ended, so we never got it completely working.

Kurt

--
Kurt Thorn
Associate Professor
Director, Nikon Imaging Center
http://thornlab.ucsf.edu/
http://nic.ucsf.edu/blog/

[Damien Alcor]

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*****

Hi Bertrand,

Actually, I already have such a cap which I bought from Sutter (through
Nikon). The problem is the control of the amount of water not to “flood”
the microscope! They only provided a pump to infuse water. It seems
difficult to me to control the amount of water while sampling multiple
positions or long time lapse.

I guess a system infusion/withdrawal would be necessary to equilibrate
the amount of water on the lens (the cap is not suited for that). Or a
system to collect the excess of water by gravity?

Have you already planned something?

Best,

Damien



Le 04/04/2016 22:31, Bertrand Vernay a écrit :

> Hi Damien
>
> I'm in touch with Sutter in the States. See below. I'm thinking of ordering one cap from them for our Nikon water 1.15 x40.
>
> Bertrand
>
>
> -----------------------------------------------------------
> From: Chris Ballard [mailto:[hidden email]]
> Sent: 16 March 2016 17:35
> To: [hidden email]
> Subject: Re: Fwd: Brass caps for Nikon objectives
>
> Hi Bertand,
>
> Good timing!   I am making one for that exact lens for a customer in Italy right now.  In fact, we usually require that the objective in question is shipped to Sutter because the tolerance as so close.  I currently have this objective on my desk and I will be designing over the next couple of weeks.
>
> We now Nickel coat these parts.  (see attached)
>
> Would you like me to send you a quote once I figure out how much each one is going to cost?  Usually they are around ~$950 but I will have a precise cost soon.
>
> Best regards,
> Chris
>
>
> --
> Bertrand Vernay, PhD
> Imaging Facility Manager
> Website: http://www.crm.ed.ac.uk/facilities/imaging
> MRC Centre for Regenerative Medicine
> SCRM Building
> The University of Edinburgh
> Edinburgh bioQuarter
> 5 Little France Drive
> Edinburgh
> EH16 4UU
>
> Office: +0044 (0)131 651 9543
>
>
>

--
Damien Alcor
Responsable Plateforme Biophotonique
Bâtiment Universitaire Archimed
INSERM U1065, C3M
151 route Saint Antoine de Ginestière
BP 2 3194
06204 NICE Cedex 3
Phone: [phone]
email:[hidden email]
http://www.unice.fr/c3m/index.php/the-facility/

[Craig Brideau]

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*****

Maybe hook it up to a syringe pump? Some of these types of pumps can be
rigged to advance the plunger a fixed amount on receipt of a triggering
pulse. This would let you 'fire' the syringe a fixed amount every time you
change position.

Craig

On Mon, Apr 4, 2016 at 3:06 PM, Damien Alcor <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Bertrand,
>
> Actually, I already have such a cap which I bought from Sutter (through
> Nikon). The problem is the control of the amount of water not to “flood”
> the microscope! They only provided a pump to infuse water. It seems
> difficult to me to control the amount of water while sampling multiple
> positions or long time lapse.
>
> I guess a system infusion/withdrawal would be necessary to equilibrate the
> amount of water on the lens (the cap is not suited for that). Or a system
> to collect the excess of water by gravity?
>
> Have you already planned something?
>
> Best,
>
> Damien
>
>
>
> Le 04/04/2016 22:31, Bertrand Vernay a écrit :
>
>> Hi Damien
>>
>> I'm in touch with Sutter in the States. See below. I'm thinking of
>> ordering one cap from them for our Nikon water 1.15 x40.
>>
>> Bertrand
>>
>>
>> -----------------------------------------------------------
>> From: Chris Ballard [mailto:[hidden email]]
>> Sent: 16 March 2016 17:35
>> To: [hidden email]
>> Subject: Re: Fwd: Brass caps for Nikon objectives
>>
>> Hi Bertand,
>>
>> Good timing!   I am making one for that exact lens for a customer in
>> Italy right now.  In fact, we usually require that the objective in
>> question is shipped to Sutter because the tolerance as so close.  I
>> currently have this objective on my desk and I will be designing over the
>> next couple of weeks.
>>
>> We now Nickel coat these parts.  (see attached)
>>
>> Would you like me to send you a quote once I figure out how much each one
>> is going to cost?  Usually they are around ~$950 but I will have a precise
>> cost soon.
>>
>> Best regards,
>> Chris
>>
>>
>> --
>> Bertrand Vernay, PhD
>> Imaging Facility Manager
>> Website: http://www.crm.ed.ac.uk/facilities/imaging
>> MRC Centre for Regenerative Medicine
>> SCRM Building
>> The University of Edinburgh
>> Edinburgh bioQuarter
>> 5 Little France Drive
>> Edinburgh
>> EH16 4UU
>>
>> Office: +0044 (0)131 651 9543
>>
>>
>>
>>
> --
> Damien Alcor
> Responsable Plateforme Biophotonique
> Bâtiment Universitaire Archimed
> INSERM U1065, C3M
> 151 route Saint Antoine de Ginestière
> BP 2 3194
> 06204 NICE Cedex 3
> Phone: [phone]
> email:[hidden email]
> http://www.unice.fr/c3m/index.php/the-facility/
>

[jmvdwin]

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*****

Hi,

Bartels Mikrotechnik
http://www.bartels-mikrotechnik.de/content/view/9/15/lang,english/ 
produced a - "Water Immersion Micro Dispenser"
for automatic supply of water immersion during experiments - for Leica.
Such device could possibly be fitted onto other brands of microscopes, I guess.

Disclosure: no commercial interest, no personal experience with such device.

JM



At 23:19 4/04/2016, you wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>Post images on http://www.imgur.com and include the link in your posting.
>*****
>
>Maybe hook it up to a syringe pump? Some of these types of pumps can be
>rigged to advance the plunger a fixed amount on receipt of a triggering
>pulse. This would let you 'fire' the syringe a fixed amount every time you
>change position.
>
>Craig
>
>On Mon, Apr 4, 2016 at 3:06 PM, Damien Alcor <[hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your posting.
> > *****
> >
> > Hi Bertrand,
> >
> > Actually, I already have such a cap which I bought from Sutter (through
> > Nikon). The problem is the control of the
> amount of water not to “flood”
> > the microscope! They only provided a pump to infuse water. It seems
> > difficult to me to control the amount of water while sampling multiple
> > positions or long time lapse.
> >
> > I guess a system infusion/withdrawal would be necessary to equilibrate the
> > amount of water on the lens (the cap is not suited for that). Or a system
> > to collect the excess of water by gravity?
> >
> > Have you already planned something?
> >
> > Best,
> >
> > Damien
> >
> >
> >
> > Le 04/04/2016 22:31, Bertrand Vernay a écrit :
> >
> >> Hi Damien
> >>
> >> I'm in touch with Sutter in the States. See below. I'm thinking of
> >> ordering one cap from them for our Nikon water 1.15 x40.
> >>
> >> Bertrand
> >>
> >>
> >> -----------------------------------------------------------
> >> From: Chris Ballard [mailto:[hidden email]]
> >> Sent: 16 March 2016 17:35
> >> To: [hidden email]
> >> Subject: Re: Fwd: Brass caps for Nikon objectives
> >>
> >> Hi Bertand,
> >>
> >> Good timing!   I am making one for that exact lens for a customer in
> >> Italy right now.  In fact, we usually require that the objective in
> >> question is shipped to Sutter because the tolerance as so close.  I
> >> currently have this objective on my desk and I will be designing over the
> >> next couple of weeks.
> >>
> >> We now Nickel coat these parts.  (see attached)
> >>
> >> Would you like me to send you a quote once I figure out how much each one
> >> is going to cost?  Usually they are around ~$950 but I will have a precise
> >> cost soon.
> >>
> >> Best regards,
> >> Chris
> >>
> >>
> >> --
> >> Bertrand Vernay, PhD
> >> Imaging Facility Manager
> >> Website: http://www.crm.ed.ac.uk/facilities/imaging
> >> MRC Centre for Regenerative Medicine
> >> SCRM Building
> >> The University of Edinburgh
> >> Edinburgh bioQuarter
> >> 5 Little France Drive
> >> Edinburgh
> >> EH16 4UU
> >>
> >> Office: +0044 (0)131 651 9543
> >>
> >>
> >>
> >>
> > --
> > Damien Alcor
> > Responsable Plateforme Biophotonique
> > Bâtiment Universitaire Archimed
> > INSERM U1065, C3M
> > 151 route Saint Antoine de Ginestière
> > BP 2 3194
> > 06204 NICE Cedex 3
> > Phone: [phone]
> > email:[hidden email]
> > http://www.unice.fr/c3m/index.php/the-facility/
> >

Jean-Marie.

Jean-Marie Vanderwinden, M.D., Ph.D.
Directeur de Recherche F.N.R.S. / Research
Director, National Fund for Scientific Research (Belgium)

Université Libre de Bruxelles (ULB)

ULB Neuroscience Institute - Neurophysiology lab
http://www.ulb.ac.be/medecine/neurophy/

Light Microscopy Facility http://limif.ulb.ac.be/

Postal address:
Laboratoire de Neurophysiologie (Prof. S.N. Schiffmann),
Faculté de Médecine, Campus Erasme, CP 601,
Université Libre de Bruxelles,
808 route de Lennik, B-1070 Brussels, Belgium.

(: Secretary: +(32).2.555.42.30, Direct: +(32).2.555.69.88
7: +(32).2.555.41.21,
. [hidden email]  

[Stephen Cody-2]

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*****

G'day Damien et al.,

I discussed my solution for a Water Dispenser on this list many years ago.
I have used various solutions for delivery, gravity feed, and syringe pump,
as well as peristaltic pump or vacuum and trap for collection of waste. I'm
currently using a syringe pump with four 50ml syringes in parallel which
can give up to four days water supply (I've not gone beyond three days).
And a vacuum line with water trap to remove excess. Rather than machine a
collar, I use poly vinyl tubing fitted over the lens. Very short lengths of
this can be purchased in hardware stores as "joiners". To hold your two
tubing lines onto the lens, small holes can be "drilled" in the side of
this tubing by rotating a hypodermic needle in your fingers.

I use to pull fine tubing next to a Bunsen to a fine tip for the irrigation
line, but these days I opt for the convenience of cutting down a gel
loading tip.

I think you'll find every thing you need to make your own in this pdf. I
have heard a while ago that a certain company was threatening to take legal
action against a small company for manufacturing such a device. However I
believe I had "published" in the public domain at Jim Pawley's course and
later on this list long before there were commercial solutions. I hope this
link works. Please let me know if it doesn't. If you do publish where the
watering device has helped I'd appreciate it if you acknowledge "Stephen H.
Cody, Monash Micro Imaging".

https://cloudstor.aarnet.edu.au/plus/index.php/s/thLe6ZNrA7C2FEz

Cheers
Steve

On 5 April 2016 at 14:57, Jean-Marie Vanderwinden <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi,
>
> Bartels Mikrotechnik
> http://www.bartels-mikrotechnik.de/content/view/9/15/lang,english/
> produced a - "Water Immersion Micro Dispenser" for automatic supply of
> water immersion during experiments - for Leica.
> Such device could possibly be fitted onto other brands of microscopes, I
> guess.
>
> Disclosure: no commercial interest, no personal experience with such
> device.
>
> JM
>
>
>
> At 23:19 4/04/2016, you wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Maybe hook it up to a syringe pump? Some of these types of pumps can be
>> rigged to advance the plunger a fixed amount on receipt of a triggering
>> pulse. This would let you 'fire' the syringe a fixed amount every time you
>> change position.
>>
>> Craig
>>
>> On Mon, Apr 4, 2016 at 3:06 PM, Damien Alcor <[hidden email]>
>> wrote:
>>
>> > *****
>> > To join, leave or search the confocal microscopy listserv, go to:
>> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> > Post images on http://www.imgur.com and include the link in your
>> posting.
>> > *****
>> >
>> > Hi Bertrand,
>> >
>> > Actually, I already have such a cap which I bought from Sutter (through
>> > Nikon). The problem is the control of the amount of water not to
>> “flood†> the microscope! They only provided a pump to infuse water. It
>> seems
>> > difficult to me to control the amount of water while sampling multiple
>> > positions or long time lapse.
>> >
>> > I guess a system infusion/withdrawal would be necessary to equilibrate
>> the
>> > amount of water on the lens (the cap is not suited for that). Or a
>> system
>> > to collect the excess of water by gravity?
>> >
>> > Have you already planned something?
>> >
>> > Best,
>> >
>> > Damien
>> >
>> >
>> >
>> > Le 04/04/2016 22:31, Bertrand Vernay a écrit :
>>
>> >
>> >> Hi Damien
>> >>
>> >> I'm in touch with Sutter in the States. See below. I'm thinking of
>> >> ordering one cap from them for our Nikon water 1.15 x40.
>> >>
>> >> Bertrand
>> >>
>> >>
>> >> -----------------------------------------------------------
>> >> From: Chris Ballard [mailto:[hidden email]]
>> >> Sent: 16 March 2016 17:35
>> >> To: [hidden email]
>> >> Subject: Re: Fwd: Brass caps for Nikon objectives
>> >>
>> >> Hi Bertand,
>> >>
>> >> Good timing!   I am making one for that exact lens for a customer in
>> >> Italy right now.  In fact, we usually require that the objective in
>> >> question is shipped to Sutter because the tolerance as so close.  I
>> >> currently have this objective on my desk and I will be designing over
>> the
>> >> next couple of weeks.
>> >>
>> >> We now Nickel coat these parts.  (see attached)
>> >>
>> >> Would you like me to send you a quote once I figure out how much each
>> one
>> >> is going to cost?  Usually they are around ~$950 but I will have a
>> precise
>> >> cost soon.
>> >>
>> >> Best regards,
>> >> Chris
>> >>
>> >>
>> >> --
>> >> Bertrand Vernay, PhD
>> >> Imaging Facility Manager
>> >> Website: http://www.crm.ed.ac.uk/facilities/imaging
>> >> MRC Centre for Regenerative Medicine
>> >> SCRM Building
>> >> The University of Edinburgh
>> >> Edinburgh bioQuarter
>> >> 5 Little France Drive
>> >> Edinburgh
>> >> EH16 4UU
>> >>
>> >> Office: +0044 (0)131 651 9543
>> >>
>> >>
>> >>
>> >>
>> > --
>> > Damien Alcor
>> > Responsable Plateforme Biophotonique
>> > Bâtiment Universitaire Archimed
>> > INSERM U1065, C3M
>> > 151 route Saint Antoine de Ginestière
>> > BP 2 3194
>> > 06204 NICE Cedex 3
>> > Phone: [phone]
>> > email:[hidden email]
>> > http://www.unice.fr/c3m/index.php/the-facility/
>> >
>>
>
> Jean-Marie.
>
> Jean-Marie Vanderwinden, M.D., Ph.D.
> Directeur de Recherche F.N.R.S. / Research Director, National Fund for
> Scientific Research (Belgium)
>
> Université Libre de Bruxelles (ULB)
>
> ULB Neuroscience Institute - Neurophysiology lab
> http://www.ulb.ac.be/medecine/neurophy/
>
> Light Microscopy Facility http://limif.ulb.ac.be/
>
> Postal address:
> Laboratoire de Neurophysiologie (Prof. S.N. Schiffmann),
> Faculté de Médecine, Campus Erasme, CP 601,
> Université Libre de Bruxelles,
> 808 route de Lennik, B-1070 Brussels, Belgium.
>
> (: Secretary: +(32).2.555.42.30, Direct: +(32).2.555.69.88
> 7: +(32).2.555.41.21,
> . [hidden email]

--
Stephen H. Cody