Well Plates for Fluorescent Studies

[B. Prabhakar Pandian]

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Hello,
              Can anyone suggest well plates with low background for
fluorescent studies.

Thanks,

-Prabhakar

--

[Kurt Thorn]

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On 6/30/2014 4:33 PM, B. Prabhakar Pandian wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello,
>              Can anyone suggest well plates with low background for
> fluorescent studies.

Most glass bottom plates should be pretty low background. You can
further decrease the background by filling the wells with sterile
filtered 0.1 M NaOH overnight and then rinsing extensively with 0.2 um
filtered water.  We have a list of plate suppliers on our wiki page:
http://nic.ucsf.edu/dokuwiki/doku.php?id=imaging_dishes

Best,
Kurt

>
> Thanks,
>
> -Prabhakar
>


--
Kurt Thorn
Director, Nikon Imaging Center
http://nic.ucsf.edu/blog/

[Casey Laris-3]

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Glass bottom plates perform the best but are expensive. The COC options
are really almost indistinguishable. Here's what we recommend most of
the time.

Recommended Plates For Imaging:
96 well: Greiner 655090
384 well: Greiner 781091

Best,

Casey

B. Prabhakar Pandian wrote:

>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello,
> Can anyone suggest well plates with low background for fluorescent
> studies.
>
> Thanks,
>
> -Prabhakar

[Rutger Schutten]

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Hi,

We use Whatman (96 well) plates from VWR for our confocal imaging which
works really well. Art no 512-1158. We used Greiner as well which works as
well but if you image upside down they are harder to seal properly in our
experience.

Best,
Rutger


2014-07-01 2:06 GMT+02:00 Casey Laris <[hidden email]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Glass bottom plates perform the best but are expensive. The COC options
> are really almost indistinguishable. Here's what we recommend most of the
> time.
>
> Recommended Plates For Imaging:
> 96 well: Greiner 655090
> 384 well: Greiner 781091
>
> Best,
>
> Casey
>
>
> B. Prabhakar Pandian wrote:
>
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Hello,
>> Can anyone suggest well plates with low background for fluorescent
>> studies.
>>
>> Thanks,
>>
>> -Prabhakar
>>
>

--
Rutger Schutten
Research engineer

Cell Profiling - Affinity Proteomics
School of Biotechnology, KTH
Science for Life Laboratory

mobile:  [phone]

Visiting address:
Tomtebodavägen 23A
171 65 Solna
Sweden

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