What did you do with your old LSM510?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi all, Ours ceased working because of yet another card breakdown and we do not feel it's worth putting another 5k EUR into it. Again. So we decided it's time. Did anyone try to convert it to a wide-field fl? Thanks, Avi -- Avi Jacob, Ph.D. Head of Light Microscopy The Mina & Everard Goodman Faculty of Life Sciences Bar-Ilan University, Ramat-Gan 5290002, Israel Cell: 052-5802544 (call here first), Desk: 972-3-5317647 http://tinyurl.com/BIU-Microscopy |
 
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George McNamara |
Re: What did you do with your old LSM510?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Avi, Yes, we converted the Zeiss LSM510 Axiovert 200M inverted microscope I managed at UMiami to widefield (internal motorized Z, manual stage). Went fine. This was several years ago, so a CCD camera was used. George On 7/6/2016 7:31 AM, Avi Jacob wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi all, > > Ours ceased working because of yet another card breakdown and we do not > feel it's worth putting another 5k EUR into it. Again. So we decided it's > time. > > Did anyone try to convert it to a wide-field fl? > > Thanks, > Avi > -- > Avi Jacob, Ph.D. > Head of Light Microscopy > The Mina & Everard Goodman Faculty of Life Sciences > Bar-Ilan University, Ramat-Gan 5290002, Israel > Cell: 052-5802544 (call here first), Desk: 972-3-5317647 > http://tinyurl.com/BIU-Microscopy -- George McNamara, PhD Houston, TX 77054 [hidden email] https://www.linkedin.com/in/georgemcnamara https://works.bepress.com/gmcnamara/75/ |
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Phillips, Thomas E. |
Re: What did you do with your old LSM510?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** We are in the process of converting our 510 to a WF scope with motorized z and motorized stage using Metamorph to drive it. We previously did this with our 5 Live scope (We now have two SP8's - one with MP, FLIM/FRET and 3 color STED, the other with digital light sheet plus a separate GSD system. Life is good!). Anyone interested in the lasers/laser control box scan head for parts can contact me. Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) [hidden email] http://www.biology.missouri.edu/faculty/phillips.html http://www.biotech.missouri.edu/mcc/ -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of George McNamara Sent: Wednesday, July 06, 2016 8:43 AM To: [hidden email] Subject: Re: What did you do with your old LSM510? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Avi, Yes, we converted the Zeiss LSM510 Axiovert 200M inverted microscope I managed at UMiami to widefield (internal motorized Z, manual stage). Went fine. This was several years ago, so a CCD camera was used. George On 7/6/2016 7:31 AM, Avi Jacob wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi all, > > Ours ceased working because of yet another card breakdown and we do > not feel it's worth putting another 5k EUR into it. Again. So we > decided it's time. > > Did anyone try to convert it to a wide-field fl? > > Thanks, > Avi > -- > Avi Jacob, Ph.D. > Head of Light Microscopy > The Mina & Everard Goodman Faculty of Life Sciences Bar-Ilan > University, Ramat-Gan 5290002, Israel > Cell: 052-5802544 (call here first), Desk: 972-3-5317647 > http://tinyurl.com/BIU-Microscopy -- George McNamara, PhD Houston, TX 77054 [hidden email] https://www.linkedin.com/in/georgemcnamara https://works.bepress.com/gmcnamara/75/ |
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DamirSudar |
Re: What did you do with your old LSM510?
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In reply to this post by Avi Jacob
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Avi, Indeed, we also converted one very old 510 into a pretty nice wide-field scope. It is one based on the Axiovert 100M and I found that Micro-Manager (https://micro-manager.org/) drives all the components on that model scope, including the dichroic block mover. Add a decent camera and good to go. (btw. Axiovision also drives all components on our scope but M-M gives more flexibility in camera choice). Best of luck, - Damir On 7/6/2016 5:31 AM, Avi Jacob wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi all, > > Ours ceased working because of yet another card breakdown and we do not > feel it's worth putting another 5k EUR into it. Again. So we decided it's > time. > > Did anyone try to convert it to a wide-field fl? > > Thanks, > Avi > -- > Avi Jacob, Ph.D. > Head of Light Microscopy > The Mina & Everard Goodman Faculty of Life Sciences > Bar-Ilan University, Ramat-Gan 5290002, Israel > Cell: 052-5802544 (call here first), Desk: 972-3-5317647 > http://tinyurl.com/BIU-Microscopy -- Damir Sudar - Affiliate Scientist Lawrence Berkeley Natl Laboratory / MBIB One Cyclotron Road, MS 977, Berkeley, CA 94720, USA T: 510/486-5346 - F: 510/486-5586 - E: [hidden email] http://biosciences.lbl.gov/profiles/damir-sudar-2/ Visiting Scientist, Oregon Health & Science University |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Damir, Did you get help from reps, or did you manage to do this on your own. What about connection to the MM computer? I have not opned it up yet, but everything seems go through the giant laser box thing. Is it a matter of just connecting cables to a computer? Thanks, Avi -- On Wed, Jul 6, 2016 at 6:31 PM, Damir Sudar <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Avi, > > Indeed, we also converted one very old 510 into a pretty nice wide-field > scope. It is one based on the Axiovert 100M and I found that Micro-Manager ( > https://micro-manager.org/) drives all the components on that model > scope, including the dichroic block mover. Add a decent camera and good to > go. (btw. Axiovision also drives all components on our scope but M-M gives > more flexibility in camera choice). > > Best of luck, > - Damir > > On 7/6/2016 5:31 AM, Avi Jacob wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> Post images on http://www.imgur.com and include the link in your posting. >> ***** >> >> Hi all, >> >> Ours ceased working because of yet another card breakdown and we do not >> feel it's worth putting another 5k EUR into it. Again. So we decided it's >> time. >> >> Did anyone try to convert it to a wide-field fl? >> >> Thanks, >> Avi >> -- >> Avi Jacob, Ph.D. >> Head of Light Microscopy >> The Mina & Everard Goodman Faculty of Life Sciences >> Bar-Ilan University, Ramat-Gan 5290002, Israel >> Cell: 052-5802544 (call here first), Desk: 972-3-5317647 >> http://tinyurl.com/BIU-Microscopy >> > > -- > Damir Sudar - Affiliate Scientist > Lawrence Berkeley Natl Laboratory / MBIB > One Cyclotron Road, MS 977, Berkeley, CA 94720, USA > T: 510/486-5346 - F: 510/486-5586 - E: [hidden email] > http://biosciences.lbl.gov/profiles/damir-sudar-2/ > > Visiting Scientist, Oregon Health & Science University > |
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George McNamara |
Re: What did you do with your old LSM510?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Avi, The real time computer (has a bunch of aluminum slats on the front) is for the confocal components only. Just disconnect from the Axiovert the scan head and any cables that go to the scanhead and real time computer. If there is another Aciovert 100 on campus you might compare cabling with that, and maybe even try Micro-Manager on that first. George On 7/10/2016 4:30 AM, Avi Jacob wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Damir, > Did you get help from reps, or did you manage to do this on your own. What > about connection to the MM computer? > I have not opned it up yet, but everything seems go through the giant > laser box thing. Is it a matter of just connecting cables to a computer? > Thanks, > Avi > > -- > > > > > > On Wed, Jul 6, 2016 at 6:31 PM, Damir Sudar <[hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> Post images on http://www.imgur.com and include the link in your posting. >> ***** >> >> Hi Avi, >> >> Indeed, we also converted one very old 510 into a pretty nice wide-field >> scope. It is one based on the Axiovert 100M and I found that Micro-Manager ( >> https://micro-manager.org/) drives all the components on that model >> scope, including the dichroic block mover. Add a decent camera and good to >> go. (btw. Axiovision also drives all components on our scope but M-M gives >> more flexibility in camera choice). >> >> Best of luck, >> - Damir >> >> On 7/6/2016 5:31 AM, Avi Jacob wrote: >> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> Post images on http://www.imgur.com and include the link in your posting. >>> ***** >>> >>> Hi all, >>> >>> Ours ceased working because of yet another card breakdown and we do not >>> feel it's worth putting another 5k EUR into it. Again. So we decided it's >>> time. >>> >>> Did anyone try to convert it to a wide-field fl? >>> >>> Thanks, >>> Avi >>> -- >>> Avi Jacob, Ph.D. >>> Head of Light Microscopy >>> The Mina & Everard Goodman Faculty of Life Sciences >>> Bar-Ilan University, Ramat-Gan 5290002, Israel >>> Cell: 052-5802544 (call here first), Desk: 972-3-5317647 >>> http://tinyurl.com/BIU-Microscopy >>> >> -- >> Damir Sudar - Affiliate Scientist >> Lawrence Berkeley Natl Laboratory / MBIB >> One Cyclotron Road, MS 977, Berkeley, CA 94720, USA >> T: 510/486-5346 - F: 510/486-5586 - E: [hidden email] >> http://biosciences.lbl.gov/profiles/damir-sudar-2/ >> >> Visiting Scientist, Oregon Health & Science University >> -- George McNamara, PhD Houston, TX 77054 [hidden email] https://www.linkedin.com/in/georgemcnamara https://works.bepress.com/gmcnamara/75/ |
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DamirSudar |
Re: What did you do with your old LSM510?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Avi, As George says, the huge box with lasers, power supplies, RTC, etc. all become surplus and any cables between the Axiovert and that box can be disconnected. I look briefly on your facility's website and noticed that your LSM 510 is built on a Axiovert 200M. That's a great scope and very well supported by MM through the ZeissCAN adapter (not the ZeissCAN29 one!!). All your motorized components will be controlled by MM. A few things I note from looking at the picture on: http://lifefaculty.biu.ac.il/FacultyInstruments/index.php?option=com_content&view=article&id=2&Itemid=71 - I cannot see where the META scanhead is connected. Is the right port? I don't know how to properly defeat the laser interlock on a 200M so you may need to ask the Zeiss technician for help on that. - It appears that you don't have a scanning stage but if you do, its controller (MCU27 or 28) will need to be plugged through a CAN bus cable into the back of the Axiovert 200M. So, a single RS232 serial cable is all you'll need between the Axiovert and the MM computer. - It looks like you have a transmitted PMT. You'll need to take that and the switcher off of the transmitted light pillar and mount the transmitted light lamp directly onto the back of the pillar. Cheers, - Damir On 7/12/2016 5:48 AM, George McNamara wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Avi, > > The real time computer (has a bunch of aluminum slats on the front) is > for the confocal components only. Just disconnect from the Axiovert > the scan head and any cables that go to the scanhead and real time > computer. > > If there is another Aciovert 100 on campus you might compare cabling > with that, and maybe even try Micro-Manager on that first. > > George > > > On 7/10/2016 4:30 AM, Avi Jacob wrote: >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> Post images on http://www.imgur.com and include the link in your >> posting. >> ***** >> >> Hi Damir, >> Did you get help from reps, or did you manage to do this on your >> own. What >> about connection to the MM computer? >> I have not opned it up yet, but everything seems go through the giant >> laser box thing. Is it a matter of just connecting cables to a computer? >> Thanks, >> Avi >> >> -- >> >> >> >> >> >> On Wed, Jul 6, 2016 at 6:31 PM, Damir Sudar <[hidden email]> wrote: >> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> Post images on http://www.imgur.com and include the link in your >>> posting. >>> ***** >>> >>> Hi Avi, >>> >>> Indeed, we also converted one very old 510 into a pretty nice >>> wide-field >>> scope. It is one based on the Axiovert 100M and I found that >>> Micro-Manager ( >>> https://micro-manager.org/) drives all the components on that model >>> scope, including the dichroic block mover. Add a decent camera and >>> good to >>> go. (btw. Axiovision also drives all components on our scope but M-M >>> gives >>> more flexibility in camera choice). >>> >>> Best of luck, >>> - Damir >>> >>> On 7/6/2016 5:31 AM, Avi Jacob wrote: >>> >>>> ***** >>>> To join, leave or search the confocal microscopy listserv, go to: >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>> Post images on http://www.imgur.com and include the link in your >>>> posting. >>>> ***** >>>> >>>> Hi all, >>>> >>>> Ours ceased working because of yet another card breakdown and we do >>>> not >>>> feel it's worth putting another 5k EUR into it. Again. So we >>>> decided it's >>>> time. >>>> >>>> Did anyone try to convert it to a wide-field fl? >>>> >>>> Thanks, >>>> Avi >>>> -- >>>> Avi Jacob, Ph.D. >>>> Head of Light Microscopy >>>> The Mina & Everard Goodman Faculty of Life Sciences >>>> Bar-Ilan University, Ramat-Gan 5290002, Israel >>>> Cell: 052-5802544 (call here first), Desk: 972-3-5317647 >>>> http://tinyurl.com/BIU-Microscopy >>>> >>> -- >>> Damir Sudar - Affiliate Scientist >>> Lawrence Berkeley Natl Laboratory / MBIB >>> One Cyclotron Road, MS 977, Berkeley, CA 94720, USA >>> T: 510/486-5346 - F: 510/486-5586 - E: [hidden email] >>> http://biosciences.lbl.gov/profiles/damir-sudar-2/ >>> >>> Visiting Scientist, Oregon Health & Science University >>> > -- Damir Sudar - Affiliate Scientist Lawrence Berkeley Natl Laboratory / MBIB One Cyclotron Road, MS 977, Berkeley, CA 94720, USA T: 510/486-5346 - F: 510/486-5586 - E: [hidden email] http://biosciences.lbl.gov/profiles/damir-sudar-2/ Visiting Scientist, Oregon Health & Science University |
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Jan-Peter Siebrasse |
Re: What did you do with your old LSM510?
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In reply to this post by Avi Jacob
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Avi, we just did this and as the other replies say, its is easily possible to use the µManager or AxioVision software to control the microscope via the serial port. HOWEVER, in our case (LSM510 META with Axiovert200M from 2006) we had to remove an additional safety shutter behind the eyepieces. This shutter could NOT be controlled by any other software but can be easily blocked in open position or completely removed. I did it myself, no need to call the service. If you need more infos/instruction how do do it, you can contact me off list. Regards, JP -- ************************************************** Dr. Jan-Peter Siebrasse [hidden email] Institut für Physikalische und Theoretische Chemie AG Biophysikalische Chemie Universität Bonn Wegeler Str. 12 53115 Bonn http://www.thch.uni-bonn.de/pc/kubitscheck/ fon +49 (0)228 - 73 2311 fax: 73 9424 |
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