Zeiss LSM710

[Claire Brown]

I was wondering if people have one or have done extensive demos on the new Zeiss LSM710. I would be interested in general feedback about the system and its performance. I am particularly interested in sensitivity and the new spectral detectors.

 

Feel free to contact me offline if you like.

 

Sincerely,

 

Claire

 

****NOTE MY NEW ADDRESS****

Claire M. Brown, PhD

Life Sciences Complex Imaging Facility Director

McGill University Department of Biochemistry

Bellini Building, Rm 137A

3649 Promenade Sir William Osler

Montreal, Quebec, H3G 0B1

514-398-4400 ext 00795 (Phone)

514-398-7452 (FAX)

[hidden email]

http://www.lifesciencescomplex.mcgill.ca/imaging

 

[Paloma Domínguez Giménez]

Dear all,

 

I write you because I am trying to install a perfusion system in an inverted Leica DMI6000 microscope and I was wondering if any of you had the same problems before.

 

Our microscope is installed in an incubator ML and it is equipped also with a heating insert P (heating stage).

This stage is a solid rectangular piece made in aluminium and is designed with a couple of openings (one in front the other) for the tubes that will allow the medium exchange.

BUT, the system does not come with a pump or the tubes for the perfusion.

 

I contacted Leica to check whether I was missing any parts of the system, but they suggested me I have to install them.

I checked then if the perfusion system FCS2 was suitable for DMI6000 microscopes, and the FCS chamber is for a circular stage, so I can not adapt that to my stage….

 

I was wondering if the solution is to buy a pump and the tubes independently.

 

Does anyone have any idea on how to solve this?

 

Thanks a lot in advanced!!!!

 

 

 

 

Paloma Dominguez, PhD.

Microscopy facility

Andalusian Center of Molecular Biology and Regenerative Medicine-CABIMER

Parque Tecnologico Cartuja 93

Avda. Americo Vespucio s/n

41092 Sevilla

Spain

Tel.:[phone]

Fax:[phone]

 

 

 

[Julio Vazquez]

Hi Paloma, 

What are your samples? are they just in a Petri dish  inside your heating insert P? Can you heat the whole incubator, or just the insert?

I can only give you some general pointers. Bioptechs has pumps and other accessories for perfusion. You may be able to build your own system from their components (pump, tubing, and needles), maybe with a bit of help from your mechanical shop:

http://www.bioptechs.com/Products/MPP/mpp.html

If that's too difficult, you could get one of their open dish systems, such as Delta T, where there are already holes for the accessories and such. If your incubator is heatable, all you need are the dishes and stage insert. They should have them for most microscope stage fittings.  If you can't heat the incubator, then you would need also the heating control unit... obviously, this would add a few thousand Euros. For more info, check their website and/or catalog:

http://www.bioptechs.com

I have no commercial interest with Bioptechs... just familiar with their products. Other vendors of microscope accessories should have similar products. I am surprised Leica wouldn't be able to help you with that. One European supplier of accessories for Leica and Zeiss microscopes is Pe-Con:

http://www.pe-con.de/pecon/index.htm

http://www.pe-con.de/lacon/interim/Main-LaCon.htm

They do have some perfusion accessories which should fit Zeiss or Leica stage inserts (your stage insert P is probably from them).

Buena suerte, 

Julio.

--

Julio Vazquez

Fred Hutchinson Cancer Research Center

Seattle, WA 98109-1024

[hidden email]

http://www.fhcrc.org/

On Feb 17, 2009, at 4:28 AM, Paloma Domínguez Giménez wrote:

Dear all,

 

I write you because I am trying to install a perfusion system in an inverted Leica DMI6000 microscope and I was wondering if any of you had the same problems before.

 

Our microscope is installed in an incubator ML and it is equipped also with a heating insert P (heating stage).

This stage is a solid rectangular piece made in aluminium and is designed with a couple of openings (one in front the other) for the tubes that will allow the medium exchange.

BUT, the system does not come with a pump or the tubes for the perfusion.

 

I contacted Leica to check whether I was missing any parts of the system, but they suggested me I have to install them.

I checked then if the perfusion system FCS2 was suitable for DMI6000 microscopes, and the FCS chamber is for a circular stage, so I can not adapt that to my stage….

 

I was wondering if the solution is to buy a pump and the tubes independently.

 

Does anyone have any idea on how to solve this?

 

Thanks a lot in advanced!!!!

 

 

 

 

Paloma Dominguez, PhD.

Microscopy facility

Andalusian Center of Molecular Biology and Regenerative Medicine-CABIMER

Parque Tecnologico Cartuja 93

Avda. Americo Vespucio s/n

41092 Sevilla

Spain

Tel.:[phone]

Fax:[phone]

 

 

 

[Aryeh Weiss]

I would like to hear from users of the Zeiss LSM700 system.
We are considering the purchase of this system, and I am wondering how users of
this system like it. Particularly, can the continuous emission beamsplitter be
used without  additional emission filters (which Zeiss suggests as a high
sensitivity mode).

--aryeh
--
Aryeh Weiss
School of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: [phone]

Israel Society for Microscopy 2009 meeting website:
http://materials.technion.ac.il/ism/ISM2009.html

[Khaled Machaca]

Claire,

We've purchased one and have been using it for a few weeks. It is a significant upgrade from the 510.  More sensitive and the spectral imaging is wonderful.

-Khaled

On Feb 17, 2009, at 1:14 AM, Claire Brown, Dr. wrote:

I was wondering if people have one or have done extensive demos on the new Zeiss LSM710. I would be interested in general feedback about the system and its performance. I am particularly interested in sensitivity and the new spectral detectors.

 

Feel free to contact me offline if you like.

 

Sincerely,

 

Claire

 

****NOTE MY NEW ADDRESS****

Claire M. Brown, PhD

Life Sciences Complex Imaging Facility Director

McGill University Department of Biochemistry

Bellini Building, Rm 137A

3649 Promenade Sir William Osler

Montreal, Quebec, H3G 0B1

514-398-4400 ext 00795 (Phone)

514-398-7452 (FAX)

[hidden email]

http://www.lifesciencescomplex.mcgill.ca/imaging

 

[Aryeh Weiss]

Have people with the LSM710 checked how close the laser lines the system  can
image? If so, I would be very interested in the results.

--aryeh
--
Aryeh Weiss
School of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: [phone]

Israel Society for Microscopy 2009 meeting website:
http://materials.technion.ac.il/ism/ISM2009.html

Khaled Machaca wrote:

> Claire,
>
> We've purchased one and have been using it for a few weeks. It is a
> significant upgrade from the 510.  More sensitive and the spectral
> imaging is wonderful.
>
> -Khaled
>
>
>
> On Feb 17, 2009, at 1:14 AM, Claire Brown, Dr. wrote:
>
>> I was wondering if people have one or have done extensive demos on the
>> new Zeiss LSM710. I would be interested in general feedback about the
>> system and its performance. I am particularly interested in
>> sensitivity and the new spectral detectors.
>>  
>> Feel free to contact me offline if you like.
>>  
>> Sincerely,
>>  
>> Claire
>>  
>> ****NOTE MY NEW ADDRESS****
>> Claire M. Brown, PhD
>> Life Sciences Complex Imaging Facility Director
>> McGill University Department of Biochemistry
>> Bellini Building, Rm 137A
>> 3649 Promenade Sir William Osler
>> Montreal, Quebec, H3G 0B1
>> 514-398-4400 ext 00795 (Phone)
>> 514-398-7452 (FAX)
>> /[hidden email]/ <mailto:[hidden email]>
>> */http://www.lifesciencescomplex.mcgill.ca/imaging/*
>>  
>

[Andreas Bruckbauer]

I guess that depends on your sample and laser power, during our demo they
imaged right over the laser lines and you could see nothing of the laser,
but this was with one of the usual super bright test samples. In this case
the laser lines are completely filtered out by the dichroics.
I later did some test on individual, multiple fluorophore labelled Fab's
(cy3) attached directly to a coverslip, you loose about 3 images (30 nm), i
had to open the pinhole a bit to get good S/N this might have affected the
laser line suppression. However the signal was impressive for a confocal.
According to Zeiss you can get the LSM 710 with special dichroics for single
molecule detection (FCS) which have double the suppression than the ususal
ones, but this is more expensive.

Andreas



On Mon, 23 Feb 2009 10:32:30 +0200, Aryeh Weiss <[hidden email]> wrote:

>Have people with the LSM710 checked how close the laser lines the system  can
>image? If so, I would be very interested in the results.
>
>--aryeh
>--
>Aryeh Weiss
>School of Engineering
>Bar Ilan University
>Ramat Gan 52900 Israel
>
>Ph:  972-3-5317638
>FAX: [phone]
>
>Israel Society for Microscopy 2009 meeting website:
>http://materials.technion.ac.il/ism/ISM2009.html
>
>Khaled Machaca wrote:
>> Claire,
>>
>> We've purchased one and have been using it for a few weeks. It is a
>> significant upgrade from the 510.  More sensitive and the spectral
>> imaging is wonderful.
>>
>> -Khaled
>>
>>
>>
>> On Feb 17, 2009, at 1:14 AM, Claire Brown, Dr. wrote:
>>
>>> I was wondering if people have one or have done extensive demos on the
>>> new Zeiss LSM710. I would be interested in general feedback about the
>>> system and its performance. I am particularly interested in
>>> sensitivity and the new spectral detectors.
>>>
>>> Feel free to contact me offline if you like.
>>>
>>> Sincerely,
>>>
>>> Claire
>>>
>>> ****NOTE MY NEW ADDRESS****
>>> Claire M. Brown, PhD
>>> Life Sciences Complex Imaging Facility Director
>>> McGill University Department of Biochemistry
>>> Bellini Building, Rm 137A
>>> 3649 Promenade Sir William Osler
>>> Montreal, Quebec, H3G 0B1
>>> 514-398-4400 ext 00795 (Phone)
>>> 514-398-7452 (FAX)
>>> /[hidden email]/ <mailto:[hidden email]>
>>> */http://www.lifesciencescomplex.mcgill.ca/imaging/*
>>>
>>

[Joe Shope]

We have an old MRC 1024 and the optic fiber is shot. Does anyone have a
source for replacing one?

Thanks,
Joe

*********************
Joe Shope
Utah State University
Department of Biology
5305 Old Main Hill
*********************

[Engstrom, Lars]

Try Oz optics in Canada.

Sent from my iPhone

On Feb 25, 2009, at 7:56 AM, "Joe Shope" <[hidden email]> wrote:

> We have an old MRC 1024 and the optic fiber is shot. Does anyone  
> have a
> source for replacing one?
>
> Thanks,
> Joe
>
> *********************
> Joe Shope
> Utah State University
> Department of Biology
> 5305 Old Main Hill
> *********************

[Craig Brideau]

Oz is good for custom stuff.  If you can get away with an off-the-shelf patch it will be cheaper.  Edmund and Thor (among others) sell these.  You just need to know the fiber type and termination options.

Craig

On Wed, Feb 25, 2009 at 10:12 AM, Engstrom, Lars <[hidden email]> wrote:

Try Oz optics in Canada.

Sent from my iPhone

On Feb 25, 2009, at 7:56 AM, "Joe Shope" <[hidden email]> wrote:

We have an old MRC 1024 and the optic fiber is shot. Does anyone have a
source for replacing one?

Thanks,
Joe

*********************
Joe Shope
Utah State University
Department of Biology
5305 Old Main Hill
*********************