Zeiss Lightsheet Z1 owners

[Ekaterina PAPUSHEVA-2]

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Dear all,

If you are using 5x/0.1 illumination objective with your Lightsheet Z1, could you tell me what is a working distance of this objective, approximately (in water)? I.e, what is the approximate distance from the front lens to the axis of an imaging objective, in original configuration?
I am asking because my calculations vary very much from the measured value...

Thanks in advance,
Ekaterina

___

Dr Ekaterina Papusheva

Bioimaging Facility

Institute of Science and Technology Austria

Phone  [phone];
Mobile [phone]
Fax    [phone]

Am Campus 1

A-3400 Klosterneuburg

Visit our website: www.ist.ac.at<http://www.ist.ac.at/>


This message and any attachment (together "the Message") are confidential and intended only for the individual named. If you have received the Message in error you should not distribute or copy this Message. Please notify the sender immediately by e-mail if you have received this Message in error and delete the Message from your system. Correspondence via e-mail is for information purposes. IST Austria neither makes nor accepts legally binding statements unless explicitly otherwise agreed to the contrary.

[Power, Rory]

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Hi Ekaterina,

I’m not familiar with exactly how the Z1 is setup but have used these lenses for custom applications.

I believe that the mechanical working distance (from the front of the objective housing) to the cover slip should be 4.376 mm. The cover glass is specified as 0.17 mm thick and the remaining distance to the focus through water should be 16 mm.

Hope that helps

Regards

Rory

…………………………………………………………

Dr. Rory Power
Associate Scientist
Morgridge Institute for Research
Medical Engineering | Huisken Lab
330 N Orchard St, Madison, WI, 53715
Tel: [phone]
[hidden email]<mailto:[hidden email]>





On Oct 12, 2018, at 08:53, Ekaterina PAPUSHEVA <[hidden email]<mailto:[hidden email]>> wrote:

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*****

Dear all,

If you are using 5x/0.1 illumination objective with your Lightsheet Z1, could you tell me what is a working distance of this objective, approximately (in water)? I.e, what is the approximate distance from the front lens to the axis of an imaging objective, in original configuration?
I am asking because my calculations vary very much from the measured value...

Thanks in advance,
Ekaterina

___

Dr Ekaterina Papusheva

Bioimaging Facility

Institute of Science and Technology Austria

Phone  [phone];
Mobile [phone]
Fax    [phone]

Am Campus 1

A-3400 Klosterneuburg

Visit our website: www.ist.ac.at<http://www.ist.ac.at/>


This message and any attachment (together "the Message") are confidential and intended only for the individual named. If you have received the Message in error you should not distribute or copy this Message. Please notify the sender immediately by e-mail if you have received this Message in error and delete the Message from your system. Correspondence via e-mail is for information purposes. IST Austria neither makes nor accepts legally binding statements unless explicitly otherwise agreed to the contrary.

[Olaf Selchow]

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Dear Ekaterina,

From what I remember from the times I was involved in the Lightsheet Z.1 at Zeiss, I can confirm Rorys answer: that this specific illumination objective lens was designed such that

- the mechanical working distance (from the front of the objective housing) to the cover slip is 4.3 mm (Rory could be correct that this is exactly
 4.376 mm, I can't recall the details to that precision ;-)

- The cover glass should definitely be  0.17 mm thick (with minimal tolerances, it is worth looking for the low tolerance coverslips, in doubt, ask Zeiss, they have them)

- and the remaining distance to the focus through water is 16 mm

This is also reflected in the size of the Lightsheet Z.1 sample chamber for water: from coverslip to coverslip it measures 32 mm ... so that the 2 opposite illumination lenses both look at the central detection axis of the imaging objective(s).

Hope this helps.
Best regards,
Olaf

[Ekaterina PAPUSHEVA-2]

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Dear Rory, fantastic, thank you!

It helps a lot!
Katja

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Power, Rory
Sent: Friday, October 12, 2018 4:25 PM
To: [hidden email]
Subject: Re: Zeiss Lightsheet Z1 owners

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*****

Hi Ekaterina,

I’m not familiar with exactly how the Z1 is setup but have used these lenses for custom applications.

I believe that the mechanical working distance (from the front of the objective housing) to the cover slip should be 4.376 mm. The cover glass is specified as 0.17 mm thick and the remaining distance to the focus through water should be 16 mm.

Hope that helps

Regards

Rory

…………………………………………………………

Dr. Rory Power
Associate Scientist
Morgridge Institute for Research
Medical Engineering | Huisken Lab
330 N Orchard St, Madison, WI, 53715
Tel: [phone]
[hidden email]<mailto:[hidden email]>





On Oct 12, 2018, at 08:53, Ekaterina PAPUSHEVA <[hidden email]<mailto:[hidden email]>> wrote:

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear all,

If you are using 5x/0.1 illumination objective with your Lightsheet Z1, could you tell me what is a working distance of this objective, approximately (in water)? I.e, what is the approximate distance from the front lens to the axis of an imaging objective, in original configuration?
I am asking because my calculations vary very much from the measured value...

Thanks in advance,
Ekaterina

___

Dr Ekaterina Papusheva

Bioimaging Facility

Institute of Science and Technology Austria

Phone  [phone];
Mobile [phone]
Fax    [phone]

Am Campus 1

A-3400 Klosterneuburg

Visit our website: www.ist.ac.at<http://www.ist.ac.at/>


This message and any attachment (together "the Message") are confidential and intended only for the individual named. If you have received the Message in error you should not distribute or copy this Message. Please notify the sender immediately by e-mail if you have received this Message in error and delete the Message from your system. Correspondence via e-mail is for information purposes. IST Austria neither makes nor accepts legally binding statements unless explicitly otherwise agreed to the contrary.

[Ekaterina PAPUSHEVA-2]

*****
To join, leave or search the confocal microscopy listserv, go to:
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*****

Dear Olaf, thanks a lot for your answer!!!

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Olaf Selchow
Sent: Sunday, October 14, 2018 2:04 PM
To: [hidden email]
Subject: Re: Zeiss Lightsheet Z1 owners

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Ekaterina,

From what I remember from the times I was involved in the Lightsheet Z.1 at Zeiss, I can confirm Rorys answer: that this specific illumination objective lens was designed such that

- the mechanical working distance (from the front of the objective housing) to the cover slip is 4.3 mm (Rory could be correct that this is exactly
 4.376 mm, I can't recall the details to that precision ;-)

- The cover glass should definitely be  0.17 mm thick (with minimal tolerances, it is worth looking for the low tolerance coverslips, in doubt, ask Zeiss, they have them)

- and the remaining distance to the focus through water is 16 mm

This is also reflected in the size of the Lightsheet Z.1 sample chamber for water: from coverslip to coverslip it measures 32 mm ... so that the 2 opposite illumination lenses both look at the central detection axis of the imaging objective(s).

Hope this helps.
Best regards,
Olaf