Zeiss vs Leica
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We are looking to upgrade our facility with a new confocal system. For the past 10 years we have used both the Leica TCSNT and SP2 systems and have been quite satisfied with their performance. We are now leaning towards the Leica SP5 but we would like to hear from others about their experiences with other confocal systems and why they think one might be better than another. We recently saw a demo of the Zeiss LSM 710 which appears to be a good system and know of some researchers who have used the 510 and love it. Most of our users have fixed samples and use an upright microscope.
Thanks for your suggestions and advice, Page -- Page Baluch, Ph.D. W.M. Keck Bioimaging Lab Manager Arizona State University/SoLS PO Box 874501 Tempe, AZ 85287-4501 |
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Hi Page,
We had a new SP5 installed mid-August 2007 and we have been happy with it. The system had the usual teething problems, but Leica was rather efficient in fixing them. The software was never Leica's strong side, and the new LAS AF is no exception, but you learn to live with it; the 1.8.2 version is definitely an improvement. The PMTs are great with a wonderful STN ratio, and the objectives are very good as well (the system shows practically no lateral or axial chromatic aberration, e.g.). I don't know the 710.
Hope this helps!
Zoltan
On Mon, Mar 31, 2008 at 7:28 PM, D. Page Baluch <[hidden email]> wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal We are looking to upgrade our facility with a new confocal system. For the past 10 years we have used both the Leica TCSNT and SP2 systems and have been quite satisfied with their performance. We are now leaning towards the Leica SP5 but we would like to hear from others about their experiences with other confocal systems and why they think one might be better than another. We recently saw a demo of the Zeiss LSM 710 which appears to be a good system and know of some researchers who have used the 510 and love it. Most of our users have fixed samples and use an upright microscope. -- -- Zoltan Cseresnyes Facility manager, Imaging Suite Dept. of Zoology University of Cambridge Downing Street, Cambridge CB2 3EJ UK Tel.: (++44) (0)1223 769282 Fax.: (++44) (0)1223 336676 |
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Page, check out also the Leica spectral excitation and spectral emission confocal SP5 X. This has a single white laser with a single laser capable of tuning excitation wavelengths at the entire visible spectrum at 1nm resolution. In addition, remember the SP5 comes with a resonant scanner too. I have personally tested a SP5 system with STED with the samples I have prepared and very pleased with the results. That means if you have a SP5 with resonant scanner you can latter upgrade to superresolution technique (STED) which can yield around 90 nm resolutions in xy. THe AOBS based system is great as you loose less photons. But, the Zeiss 710 system's new grating design is worth to have a look. I personally feel that the Zeiss 710 is a system step cloaser to a spectral confocal such as an SP5. Zeiss has great flexibility in your laser selection and that will let you not burn your wallet upfront and you can add lasers latter as and when it is needed. No commercial interest on either vendors. I feel both should be great systems and you should select depending on your necessity and your budget. Shiv At 02:40 PM 3/31/2008, you wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Microscopy Facility Manager 8, Institute for Genomic Biology University of Illinois at Urbana-Champaign 1206 West Gregory Dr. Urbana, IL 61801 USA Office: 217.333.1214 Fax: 217.244.2496 [hidden email] http://core.igb.uiuc.edu |
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Search the CONFOCAL archive at
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You need to consider if you are doing any spectroscopy
work. I am not sure how the new Zeiss spectral system works but the LSM510
meta scans the whole spectrum simultaneously where as the Leica does this
sequentially. If you have photobleaching considerations this could affect
your choice. I have used both and for most needs either should suit very
well. From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Mayandi Sivaguru Sent: Tuesday, 1 April 2008 3:55 p.m. To: [hidden email] Subject: Re: Zeiss vs Leica Page, check out also the Leica spectral excitation and spectral emission confocal SP5 X. This has a single white laser with a single laser capable of tuning excitation wavelengths at the entire visible spectrum at 1nm resolution. In addition, remember the SP5 comes with a resonant scanner too. I have personally tested a SP5 system with STED with the samples I have prepared and very pleased with the results. That means if you have a SP5 with resonant scanner you can latter upgrade to superresolution technique (STED) which can yield around 90 nm resolutions in xy. THe AOBS based system is great as you loose less photons. But, the Zeiss 710 system's new grating design is worth to have a look. I personally feel that the Zeiss 710 is a system step cloaser to a spectral confocal such as an SP5. Zeiss has great flexibility in your laser selection and that will let you not burn your wallet upfront and you can add lasers latter as and when it is needed. No commercial interest on either vendors. I feel both should be great systems and you should select depending on your necessity and your budget. Shiv At 02:40 PM 3/31/2008, you wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Microscopy Facility Manager 8, Institute for Genomic Biology University of Illinois at Urbana-Champaign 1206 West Gregory Dr. Urbana, IL 61801 USA Office: 217.333.1214 Fax: 217.244.2496 [hidden email] http://core.igb.uiuc.edu DISCLAIMER: |
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In reply to this post by Mayandi Sivaguru
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Just a correction ......... Leica SP5X tunable white light laser system covers 470-670 nm only, not the entire visible spectrum. Very stable, provide 1.5 mW per nm, need more power... increase the bandwidth. ammasi On Mon, 31 Mar 2008 21:55:26 -0500 Mayandi Sivaguru <[hidden email]> wrote: > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > > Page, check out also the Leica spectral excitation and spectral emission >confocal SP5 X. This has a single white laser with a single laser capable >of tuning excitation wavelengths at the entire visible spectrum at 1nm >resolution. > In addition, remember the SP5 comes with a resonant scanner too. I have >personally tested a SP5 system with STED with the samples I have prepared >and very pleased with the results. That means if you have a SP5 with >resonant scanner you can latter upgrade to superresolution technique (STED) >which can yield around 90 nm resolutions in xy. THe AOBS based system is >great as you loose less photons. > But, the Zeiss 710 system's new grating design is worth to have a look. I >personally feel that the Zeiss 710 is a system step cloaser to a spectral >confocal such as an SP5. Zeiss has great flexibility in your laser >selection and that will let you not burn your wallet upfront and you can >add lasers latter as and when it is needed. > No commercial interest on either vendors. I feel both should be great >systems and you should select depending on your necessity and your budget. > Shiv > > > At 02:40 PM 3/31/2008, you wrote: >>Search the CONFOCAL archive at >>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >>Hi Page, >> >>We had a new SP5 installed mid-August 2007 and we have been happy with it. >> The system had the usual teething problems, but Leica was rather >>efficient in fixing them. The software was never Leica's strong side, and >>the new LAS AF is no exception, but you learn to live with it; the 1.8.2 >>version is definitely an improvement. The PMTs are great with a wonderful >>STN ratio, and the objectives are very good as well (the system shows >>practically no lateral or axial chromatic aberration, e.g.). I don't know >>the 710. >>Hope this helps! >> >>Zoltan >> >>On Mon, Mar 31, 2008 at 7:28 PM, D. Page Baluch >><<mailto:[hidden email]>[hidden email]> wrote: >>Search the CONFOCAL archive at >><http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >>We are looking to upgrade our facility with a new confocal system. For the >>past 10 years we have used both the Leica TCSNT and SP2 systems and have >>been quite satisfied with their performance. We are now leaning towards the >>Leica SP5 but we would like to hear from others about their experiences >>with other confocal systems and why they think one might be better than >>another. We recently saw a demo of the Zeiss LSM 710 which appears to be a >>good system and know of some researchers who have used the 510 and love it. >>Most of our users have fixed samples and use an upright microscope. >>Thanks for your suggestions and advice, >>Page >> >>-- >>Page Baluch, Ph.D. >>W.M. Keck Bioimaging Lab Manager >>Arizona State University/SoLS >>PO Box 874501 >>Tempe, AZ 85287-4501 >> >> >> >> >>-- >>-- >>Zoltan Cseresnyes >>Facility manager, Imaging Suite >>Dept. of Zoology University of Cambridge >>Downing Street, Cambridge >>CB2 3EJ UK >> >>Tel.: (++44) (0)1223 769282 >>Fax.: (++44) (0)1223 336676 > > Mayandi Sivaguru, PhD, PhD > Microscopy Facility Manager > 8, Institute for Genomic Biology > University of Illinois at Urbana-Champaign > 1206 West Gregory Dr. > Urbana, IL 61801 USA > > Office: 217.333.1214 >Fax: 217.244.2496 > [hidden email] > http://core.igb.uiuc.edu > Ammasi Periasamy, Ph.D. Director, Keck Center for Cellular Imaging (KCCI) Professor of Biology and Biomedical Engineering Biology, Gilmer Hall (064), McCormick Rd University of Virginia Charlottesville, VA 22904 Voice: 434-243-7602 (Office); 982-4869 (lab) Fax:434-982-5210; Email:[hidden email] http//:www.kcci.virginia.edu ************************ Workshop on FRET Microscopy, March 3-7, 2009 http://www.kcci.virginia.edu/workshop/workshop2009/index.php ************************* |
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > Do they offer the option of violet (or other) lasers in addition to the supercontinum laser? That would extend it beyond the 470-670 limits? The brochure also mentions cooled PMTs - I wonder what real-world impact that will have? Regards, Adrian Smith Centenary Institute, Sydney, Australia > > Just a correction ......... > Leica SP5X tunable white light laser system covers 470-670 nm only, > not the entire visible spectrum. Very stable, provide 1.5 mW per nm, > need more power... increase the bandwidth. > ammasi > > > > On Mon, 31 Mar 2008 21:55:26 -0500 > Mayandi Sivaguru <[hidden email]> wrote: >> Search the CONFOCAL archive at >> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >> Page, check out also the Leica spectral excitation and spectral >> emission confocal SP5 X. This has a single white laser with a >> single laser capable of tuning excitation wavelengths at the entire >> visible spectrum at 1nm resolution. >> In addition, remember the SP5 comes with a resonant scanner too. I >> have personally tested a SP5 system with STED with the samples I >> have prepared and very pleased with the results. That means if you >> have a SP5 with resonant scanner you can latter upgrade to >> superresolution technique (STED) which can yield around 90 nm >> resolutions in xy. THe AOBS based system is great as you loose less >> photons. >> But, the Zeiss 710 system's new grating design is worth to have a >> look. I personally feel that the Zeiss 710 is a system step cloaser >> to a spectral confocal such as an SP5. Zeiss has great flexibility >> in your laser selection and that will let you not burn your wallet >> upfront and you can add lasers latter as and when it is needed. >> No commercial interest on either vendors. I feel both should be >> great systems and you should select depending on your necessity and >> your budget. >> Shiv >> At 02:40 PM 3/31/2008, you wrote: >>> Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >>> Hi Page, >>> >>> We had a new SP5 installed mid-August 2007 and we have been happy >>> with it. The system had the usual teething problems, but Leica >>> was rather efficient in fixing them. The software was never >>> Leica's strong side, and the new LAS AF is no exception, but you >>> learn to live with it; the 1.8.2 version is definitely an >>> improvement. The PMTs are great with a wonderful STN ratio, and >>> the objectives are very good as well (the system shows practically >>> no lateral or axial chromatic aberration, e.g.). I don't know the >>> 710. >>> Hope this helps! >>> >>> Zoltan >>> >>> On Mon, Mar 31, 2008 at 7:28 PM, D. Page Baluch <<mailto:[hidden email] >>> >[hidden email]> wrote: >>> Search the CONFOCAL archive at <http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >>> >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal We are >>> looking to upgrade our facility with a new confocal system. For >>> the past 10 years we have used both the Leica TCSNT and SP2 >>> systems and have been quite satisfied with their performance. We >>> are now leaning towards the Leica SP5 but we would like to hear >>> from others about their experiences with other confocal systems >>> and why they think one might be better than another. We recently >>> saw a demo of the Zeiss LSM 710 which appears to be a good system >>> and know of some researchers who have used the 510 and love it. >>> Most of our users have fixed samples and use an upright microscope. >>> Thanks for your suggestions and advice, >>> Page >>> >>> -- >>> Page Baluch, Ph.D. >>> W.M. Keck Bioimaging Lab Manager >>> Arizona State University/SoLS >>> PO Box 874501 >>> Tempe, AZ 85287-4501 >>> >>> >>> >>> >>> -- >>> -- >>> Zoltan Cseresnyes >>> Facility manager, Imaging Suite >>> Dept. of Zoology University of Cambridge >>> Downing Street, Cambridge >>> CB2 3EJ UK >>> >>> Tel.: (++44) (0)1223 769282 >>> Fax.: (++44) (0)1223 336676 >> Mayandi Sivaguru, PhD, PhD >> Microscopy Facility Manager >> 8, Institute for Genomic Biology >> University of Illinois at Urbana-Champaign >> 1206 West Gregory Dr. >> Urbana, IL 61801 USA >> Office: 217.333.1214 >> Fax: 217.244.2496 >> [hidden email] >> http://core.igb.uiuc.edu > > Ammasi Periasamy, Ph.D. > Director, Keck Center for Cellular Imaging (KCCI) > Professor of Biology and Biomedical Engineering > Biology, Gilmer Hall (064), McCormick Rd > University of Virginia > Charlottesville, VA 22904 > Voice: 434-243-7602 (Office); 982-4869 (lab) > Fax:434-982-5210; Email:[hidden email] > http//:www.kcci.virginia.edu > ************************ > Workshop on FRET Microscopy, March 3-7, 2009 > http://www.kcci.virginia.edu/workshop/workshop2009/index.php > ************************* |
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear all, afaik one can combine the white laser with the standard laser module. Don't ask me about the details, but this offers possibilities if one needs more power for bleaching, or additional wavelengths next to 470-670nm. There are some fundamental differences between SP5 and LSM710: -SP5: white laser, AOBS, STED -LSM710: option for two scanners, simultaneous spectral detection Yes, the SP5 detectors are water-cooled. The performance in terms of signal-to-noise is really nice. But so far I have no comparison to LSM710 (Zeiss claims it's MUCH better than LSM510...). Personally I found the SP5 to be more user-friendly compared to the LSM510 (straight forward layout and usage); whereas the Zeiss (LSM510) feels more "scientific" (more values/details given, more complex hardware). Usage on software-level should be now easier with the Zeiss ZEN software, unfortunately the first version introduced some instabilities. Both systems offer fast scanners, which might be interesting for fixed samples as well (more time-efficient imaging of big/thick samples). Both systems have single pinholes, so colocalization in the visible range should not be that tricky. I am still not sure about the laser coupling of LSM710 - this is now done in the scanhead after the fiber, so how do they deal with the alignment over multiple wavelengths? The implementation of the Hamamatsu detector in the old Meta was not perfect (low sensitivity and signal-to-noise), however, with bright samples still the second-fastest solution after Nikon. One needs to test if the new version in the LSM710 does perform better. The beam-path looks promising. Michael > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >> > > > Do they offer the option of violet (or other) lasers in addition to > the supercontinum laser? That would extend it beyond the 470-670 limits? > > The brochure also mentions cooled PMTs - I wonder what real-world > impact that will have? > > Regards, > > Adrian Smith > Centenary Institute, Sydney, Australia > > > >> >> Just a correction ......... >> Leica SP5X tunable white light laser system covers 470-670 nm only, >> not the entire visible spectrum. Very stable, provide 1.5 mW per nm, >> need more power... increase the bandwidth. >> ammasi >> >> >> >> On Mon, 31 Mar 2008 21:55:26 -0500 >> Mayandi Sivaguru <[hidden email]> wrote: >>> Search the CONFOCAL archive at >>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >>> Page, check out also the Leica spectral excitation and spectral >>> emission confocal SP5 X. This has a single white laser with a >>> single laser capable of tuning excitation wavelengths at the entire >>> visible spectrum at 1nm resolution. >>> In addition, remember the SP5 comes with a resonant scanner too. I >>> have personally tested a SP5 system with STED with the samples I >>> have prepared and very pleased with the results. That means if you >>> have a SP5 with resonant scanner you can latter upgrade to >>> superresolution technique (STED) which can yield around 90 nm >>> resolutions in xy. THe AOBS based system is great as you loose less >>> photons. >>> But, the Zeiss 710 system's new grating design is worth to have a >>> look. I personally feel that the Zeiss 710 is a system step cloaser >>> to a spectral confocal such as an SP5. Zeiss has great flexibility >>> in your laser selection and that will let you not burn your wallet >>> upfront and you can add lasers latter as and when it is needed. >>> No commercial interest on either vendors. I feel both should be >>> great systems and you should select depending on your necessity and >>> your budget. >>> Shiv >>> At 02:40 PM 3/31/2008, you wrote: >>>> Search the CONFOCAL archive at >>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >>>> Hi Page, >>>> >>>> We had a new SP5 installed mid-August 2007 and we have been happy >>>> with it. The system had the usual teething problems, but Leica >>>> was rather efficient in fixing them. The software was never >>>> Leica's strong side, and the new LAS AF is no exception, but you >>>> learn to live with it; the 1.8.2 version is definitely an >>>> improvement. The PMTs are great with a wonderful STN ratio, and >>>> the objectives are very good as well (the system shows practically >>>> no lateral or axial chromatic aberration, e.g.). I don't know the >>>> 710. >>>> Hope this helps! >>>> >>>> Zoltan >>>> >>>> On Mon, Mar 31, 2008 at 7:28 PM, D. Page Baluch >>>> <<mailto:[hidden email] >>>> >[hidden email]> wrote: >>>> Search the CONFOCAL archive at >>>> <http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >>>> >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal We are >>>> looking to upgrade our facility with a new confocal system. For >>>> the past 10 years we have used both the Leica TCSNT and SP2 >>>> systems and have been quite satisfied with their performance. We >>>> are now leaning towards the Leica SP5 but we would like to hear >>>> from others about their experiences with other confocal systems >>>> and why they think one might be better than another. We recently >>>> saw a demo of the Zeiss LSM 710 which appears to be a good system >>>> and know of some researchers who have used the 510 and love it. >>>> Most of our users have fixed samples and use an upright microscope. >>>> Thanks for your suggestions and advice, >>>> Page >>>> >>>> -- >>>> Page Baluch, Ph.D. >>>> W.M. Keck Bioimaging Lab Manager >>>> Arizona State University/SoLS >>>> PO Box 874501 >>>> Tempe, AZ 85287-4501 >>>> >>>> >>>> >>>> >>>> -- >>>> -- >>>> Zoltan Cseresnyes >>>> Facility manager, Imaging Suite >>>> Dept. of Zoology University of Cambridge >>>> Downing Street, Cambridge >>>> CB2 3EJ UK >>>> >>>> Tel.: (++44) (0)1223 769282 >>>> Fax.: (++44) (0)1223 336676 >>> Mayandi Sivaguru, PhD, PhD >>> Microscopy Facility Manager >>> 8, Institute for Genomic Biology >>> University of Illinois at Urbana-Champaign >>> 1206 West Gregory Dr. >>> Urbana, IL 61801 USA >>> Office: 217.333.1214 >>> Fax: 217.244.2496 >>> [hidden email] >>> http://core.igb.uiuc.edu >> >> Ammasi Periasamy, Ph.D. >> Director, Keck Center for Cellular Imaging (KCCI) >> Professor of Biology and Biomedical Engineering >> Biology, Gilmer Hall (064), McCormick Rd >> University of Virginia >> Charlottesville, VA 22904 >> Voice: 434-243-7602 (Office); 982-4869 (lab) >> Fax:434-982-5210; Email:[hidden email] >> http//:www.kcci.virginia.edu >> ************************ >> Workshop on FRET Microscopy, March 3-7, 2009 >> http://www.kcci.virginia.edu/workshop/workshop2009/index.php >> ************************* |
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal On 01/04/2008, at 7:19 PM, Michael Weber wrote: > Yes, the SP5 detectors are water-cooled. The performance in terms of > signal-to-noise is really nice Is that true of the "standard" SP5 or is that something new with the SP5 X? I don't recall seeing anything in the SP5 literature about water cooling (or cooling or any sort) but I might have missed it? Is anyone else using cooled PMTs? Also I noticed the SP5 X brochure that Leica is claiming "best red emission detection available" - does anyone know what is behind this claim, ie have they done something new with the SP5 X or would they also make that claim of the "standard" SP5? Regards, Adrian Smith Centenary Institute, Sydney, Australia |
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Adrian, every SP5 has water-cooled detectors. Part of the scan-head electronics (detector read-out?) also gets cooled. I don't know why Leica doesn't put this on the feature list. I am sure they have a patent, so they shouldn't be afraid ;). Maybe they think that it's not important for the ordinary customer? To my understanding there are no new technical developments in the X except the implementation of the superk laser. I cannot say anything about the far-red performance, expect that it's good. But no quantitative results so far. Leica people are on this list too, so maybe they can comment on that? Michael Adrian Smith wrote: > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > On 01/04/2008, at 7:19 PM, Michael Weber wrote: > >> Yes, the SP5 detectors are water-cooled. The performance in terms of >> signal-to-noise is really nice > > > Is that true of the "standard" SP5 or is that something new with the SP5 > X? I don't recall seeing anything in the SP5 literature about water > cooling (or cooling or any sort) but I might have missed it? Is anyone > else using cooled PMTs? > > Also I noticed the SP5 X brochure that Leica is claiming "best red > emission detection available" - does anyone know what is behind this > claim, ie have they done something new with the SP5 X or would they also > make that claim of the "standard" SP5? > > Regards, > > Adrian Smith > Centenary Institute, Sydney, Australia |
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In reply to this post by Adrian Smith-6
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi, The SP5 has water cooled pmts but I think is room temp. Actually I'm also thinking on making a cooling system for the new external PMTs that we will attach to a biorad1024. It will be active cooling with Peltier elements. I expect this would decrease dark current which will enable to push a bit the electronic gain. Biggest problem is condensation... Did anyone make something similar? Regards, NM Adrian Smith wrote: > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > On 01/04/2008, at 7:19 PM, Michael Weber wrote: > >> Yes, the SP5 detectors are water-cooled. The performance in terms of >> signal-to-noise is really nice > > > Is that true of the "standard" SP5 or is that something new with the SP5 > X? I don't recall seeing anything in the SP5 literature about water > cooling (or cooling or any sort) but I might have missed it? Is anyone > else using cooled PMTs? > > Also I noticed the SP5 X brochure that Leica is claiming "best red > emission detection available" - does anyone know what is behind this > claim, ie have they done something new with the SP5 X or would they also > make that claim of the "standard" SP5? > > Regards, > > Adrian Smith > Centenary Institute, Sydney, Australia > -- Nuno Moreno Cell Imaging Unit Instituto Gulbenkian de Ciência http://uic.igc.gulbekian.pt http://www.igc.gulbekian.pt phone +351 214464606 fax +351 214407970 |
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In reply to this post by Page Baluch
Search the CONFOCAL archive at
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Commercial Vendor Response
All,
just to confirm that the White Light Laser in the Leica TCS SP5X can
be combined with e.g. an Argon Laser for high-power photobleaching
experiments - this is done by an AOBM (Acousto-Optical Beam Merger)
Module, which is part of the White Light Laser Module.
Combination with 405 nm/365nm excitation is possible as well. The Leica TCS SP5 X can handle up to 8 freely
definable simultaneous excitation wavelengths and intensities for optimally
matching the excitation requirements of virtually any dye.
The Leica TCS SP5 Electronics and Detectors are cooled by a closed-loop
cooling system for optimised Signal-to-Noise ratio.
Equipped with resonant scanner, the system also offers high speed true confocal imaging @ up to 16000 lines per second. Best regards
Martin
Martin Hoppe, Ph.D.
Head of Market Management Life Science Division
Leica Microsystems CMS GmbH
Am Friedensplatz 3
D-68165 Mannheim
+49-172-6230409
In a message dated 01.04.2008 10:19:48 Mitteleuropäische Sommerzeit,
[hidden email] writes:
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