alternative dye for ICG using shorter excitation wavelength

[Ko, Alex]

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Hi ,

We have been working with ICG dyes to track blood flow in vivo using a 806nm laser light, however our new equipment does not work very well with such long wavelength, I am wondering if anyone knows about an alternative dye which binds with plasma protein but using a shorter excitation wavelength ( like in the upper visible region 660-735nm range ), thanks in advance.

Alex

[Craig Brideau]

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I've had some success using IRDye 680LT for confocal imaging.  It has a
fairly broad absorption spectrum and I've used it with a 640nm diode laser
with some success.  It is quite photostable as well, but you will have to
see if they can get it conjugated for what you want.

Craig Brideau


On Thu, Mar 27, 2014 at 5:17 PM, Ko, Alex <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi ,
>
> We have been working with ICG dyes to track blood flow in vivo using a
> 806nm laser light, however our new equipment does not work very well with
> such long wavelength, I am wondering if anyone knows about an alternative
> dye which binds with plasma protein but using a shorter excitation
> wavelength ( like in the upper visible region 660-735nm range ), thanks in
> advance.
>
> Alex

[Armstrong, Brian]

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Hi, you could try dextran conjugates. Molecular Probes has dextrans in many different spectra. Of course dextran is a sugar (polysaccharide) and does not specifically bind to plasma.
Cheers,

Brian Armstrong PhD
Director, Light Microscopy Core
x62872

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ko, Alex
Sent: Thursday, March 27, 2014 4:18 PM
To: [hidden email]
Subject: alternative dye for ICG using shorter excitation wavelength

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Hi ,

We have been working with ICG dyes to track blood flow in vivo using a 806nm laser light, however our new equipment does not work very well with such long wavelength, I am wondering if anyone knows about an alternative dye which binds with plasma protein but using a shorter excitation wavelength ( like in the upper visible region 660-735nm range ), thanks in advance.

Alex


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[Rainer Kohler-2]

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Hi,

We use Angiosense680 or Angiospark 680 but it is expensive.


Rainer

On Mar 27, 2014, at 7:42 PM, Armstrong, Brian <[hidden email]<mailto:[hidden email]>> wrote:

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Hi, you could try dextran conjugates. Molecular Probes has dextrans in many different spectra. Of course dextran is a sugar (polysaccharide) and does not specifically bind to plasma.
Cheers,

Brian Armstrong PhD
Director, Light Microscopy Core
x62872

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ko, Alex
Sent: Thursday, March 27, 2014 4:18 PM
To: [hidden email]
Subject: alternative dye for ICG using shorter excitation wavelength

*****
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Hi ,

We have been working with ICG dyes to track blood flow in vivo using a 806nm laser light, however our new equipment does not work very well with such long wavelength, I am wondering if anyone knows about an alternative dye which binds with plasma protein but using a shorter excitation wavelength ( like in the upper visible region 660-735nm range ), thanks in advance.

Alex


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Rainer Kohler, Ph.D
Microscopist
Center for Systems Biology
Massachusetts General Hospital
Richard B. Simches Research Center
185 Cambridge Street
Suite 5.210
Boston, MA 02114
Phone.: [phone]
Cell phone: [phone]



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[George McNamara]

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http://www.perkinelmer.com/Catalog/Category/ID/Vascular

AngioSense 680 etc (Alexa Fluor 680 on something)

see also

http://www.perkinelmer.com/Catalog/Category/ID/Fluorescence%20Agents%20Portfolio

for lighting up more body parts. These are from VisEn Medical, which was
bought by Caliper Life Sciences which was bought by PerkinElmer ...
which we'll have to see if it gets bought by Thermo Fisher someday. At
least they shouldn't need to divest their NIRF line since it is sourced
from T.F. (and then GE Healthcare will but T.F. and Neutron Jack 2.0
will split out the loser business units).

Enjoy,

George


On 3/27/2014 6:42 PM, Armstrong, Brian wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi, you could try dextran conjugates. Molecular Probes has dextrans in many different spectra. Of course dextran is a sugar (polysaccharide) and does not specifically bind to plasma.
> Cheers,
>
> Brian Armstrong PhD
> Director, Light Microscopy Core
> x62872
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ko, Alex
> Sent: Thursday, March 27, 2014 4:18 PM
> To: [hidden email]
> Subject: alternative dye for ICG using shorter excitation wavelength
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi ,
>
> We have been working with ICG dyes to track blood flow in vivo using a 806nm laser light, however our new equipment does not work very well with such long wavelength, I am wondering if anyone knows about an alternative dye which binds with plasma protein but using a shorter excitation wavelength ( like in the upper visible region 660-735nm range ), thanks in advance.
>
> Alex
>
>
> ---------------------------------------------------------------------
> *SECURITY/CONFIDENTIALITY WARNING:
> This message and any attachments are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. (fpc5p)
> ---------------------------------------------------------------------
>
>    

--



George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
Tattletales http://works.bepress.com/gmcnamara/26/