argon laser refurbishing

[Sylvie Le Guyader]

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Dear all

We have little experience with refurbishing argon lasers. We only have 1 refurbished argon and this was done only a couple of months ago.
Now that we are in need of refurbishing one again, our local vendor claims that refurbished argon lasers last a lot less time than new ones and often stop working after one year.
I have search the list archives without finding any comparison of the lifetime of refurbished vs new argon lasers.

What is your experience with refurbished argon lasers? Do they last less than new argons?

thanks

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Hälsovägen 7C,
Room 7362/7840
14157 Huddinge, Sweden
mobile: [phone]
LCI website<http://ki.se/en/bionut/welcome-to-the-lci-facility>

[Craig Brideau]

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The things that usually go on an Argon are the electrodes in the gas tube
or the high-voltage power supply. When you get an argon refurbished the gas
tube is replaced, so you get fresh electrodes, and a fresh batch of gas at
the correct pressure. Assuming the cavity mirrors are relatively clean then
the new tube basically makes it like a new laser. The exception is if you
are having problems with the power supply, although the repair company can
also deal with issues there by replacing capacitors, etc.

Craig

On Tue, Feb 6, 2018 at 4:21 AM, Sylvie Le Guyader <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear all
>
> We have little experience with refurbishing argon lasers. We only have 1
> refurbished argon and this was done only a couple of months ago.
> Now that we are in need of refurbishing one again, our local vendor claims
> that refurbished argon lasers last a lot less time than new ones and often
> stop working after one year.
> I have search the list archives without finding any comparison of the
> lifetime of refurbished vs new argon lasers.
>
> What is your experience with refurbished argon lasers? Do they last less
> than new argons?
>
> thanks
>
> Med vänlig hälsning / Best regards
>
> Sylvie
>
> @@@@@@@@@@@@@@@@@@@@@@@@
> Sylvie Le Guyader, PhD
> Live Cell Imaging Facility Manager
> Karolinska Institutet- Bionut Dpt
> Hälsovägen 7C,
> Room 7362/7840
> 14157 Huddinge, Sweden
> mobile: [phone]
> LCI website<http://ki.se/en/bionut/welcome-to-the-lci-facility>
>

[Arvydas Matiukas]

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Dear microscopists,
 
I am looking for  a  LSM510/710 sscanhead  with optics/filters/detectors
supporting at least 405/488/543/633 laser lines and two PMT detectors.
 
 A working condition is strongly preferred, as well as accompanying acquisition electronics
module though non working condition also may be considered for cheap price - maybe it
would be possible to combine parts from several into single working scanhead.
 
Thanks,
Arvydas
**********************
 
 
 
Arvydas Matiukas, Ph.D.
Director of Confocal&Two-Photon Core
SUNY Upstate Medical University
Neuroscience & Physiology Dept

Syracuse, NY
 

[Arvydas Matiukas]

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Hello Microscopists,
 
Please share you thoughts/experience what would be an inexpensive way
to upgrade
510 META NLO on the upright Axioskop 2 FS mot stand. i am aiming at the
most sensitive
available detectors( Idealy 2x GaAsP  for two colors). I would prefer
keep the 2p Chameleon laser
and the upright stand. By inexpensive plan I assume a fraction of the
Thorlabs 2p scope price ($100k).
Fitting into $20k budget would be perfect.
 
Initially I was thinking to reconnect the priginal LSM510 META NLO
scanhead to tothe upright stand,
however, some people working with 2p setups pointed to me that the
sensitivity of such system won't
be good enough for the most deep tissue imaging projects. Therefore I
am wondering if it is possible
to significantly improve sensitivity just by replacing old PMT/NDD
detectors, or optics/beampath still be
an issue .
 
Thanks,
Arvydas
***************************
 
 


 
 
Arvydas Matiukas, Ph.D.
Director of Confocal&Two-Photon Core
SUNY Upstate Medical University
Neuroscience & Physiology Dept

Syracuse, NY
 >>> Sylvie Le Guyader <[hidden email]> 2/6/2018 6:21 AM >>>
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posting.
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Dear all

We have little experience with refurbishing argon lasers. We only have
1 refurbished argon and this was done only a couple of months ago.
Now that we are in need of refurbishing one again, our local vendor
claims that refurbished argon lasers last a lot less time than new ones
and often stop working after one year.
I have search the list archives without finding any comparison of the
lifetime of refurbished vs new argon lasers.

What is your experience with refurbished argon lasers? Do they last
less than new argons?

thanks

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Hälsovägen 7C,
Room 7362/7840
14157 Huddinge, Sweden
mobile: [phone]
LCI website<http://ki.se/en/bionut/welcome-to-the-lci-facility>

[George McNamara]

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*****

Hi Arvydas,

I suggest investing in GPU deconvolution, for example,
www.microvolution.com or SVI Huygens (www.svi.nl) or AutoQuant. All
three commercial software give comparable results (I trialed each summer
2017 on an NVidia GTX 1060 on my office PC ... and then another division
dropped in an Leica SP8 + HyVolution2 [SVI] instrument to keep me busy).

See the example image pair on top of page 2 (ignoring the cover page) of
McTips 2017 download

https://works.bepress.com/gmcnamara/81



I am still very interested in buying a GPU deconvolution license and
having "drop&drag" instant gratification on our campus intranet ...
ideally everyone who publishes would acknowledge our image core, parent
P30 center grant and any S10 grants (and other funding sources) to fund
instruments used in the research.

With respect to S10 grants: my thanks to the study section that awarded
our image core's proposal a score in the funding range (I started work a
few days before it was submitted, so all the credit goes to Brian, Olga,
Mark, Bin, John Gibas, our users, and Jason Brenner of Olympus [John is
now also at Olympus]). Hopefully the new instrument will be installed
and operational before the Microscopy Society of America (MSA) meeting
in August here in Baltimore and we can have meeting attendees over.

https://www.microscopy.org/MandM/2018

George

p.s. disclosure: a very nice dataset that I 'clicked' is on the gallery
at https://www.microvolution.com/gallery (no, Marc Bruce did not buy me
a new Tesla, or even send me a matchbox Tesla, nor any other 'direct
deposit').  I also recommend the TOP1 single molecule RNA FISH  dataset
(likely Stellaris RNA FISH probe set) acquired by Dane Maxfield.



On 2/15/2018 2:06 PM, Arvydas Matiukas wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello Microscopists,
>  
> Please share you thoughts/experience what would be an inexpensive way
> to upgrade
> 510 META NLO on the upright Axioskop 2 FS mot stand. i am aiming at the
> most sensitive
> available detectors( Idealy 2x GaAsP  for two colors). I would prefer
> keep the 2p Chameleon laser
> and the upright stand. By inexpensive plan I assume a fraction of the
> Thorlabs 2p scope price ($100k).
> Fitting into $20k budget would be perfect.
>  
> Initially I was thinking to reconnect the priginal LSM510 META NLO
> scanhead to tothe upright stand,
> however, some people working with 2p setups pointed to me that the
> sensitivity of such system won't
> be good enough for the most deep tissue imaging projects. Therefore I
> am wondering if it is possible
> to significantly improve sensitivity just by replacing old PMT/NDD
> detectors, or optics/beampath still be
> an issue .
>  
> Thanks,
> Arvydas
> ***************************
>  
>  
>
>
>  
>  
> Arvydas Matiukas, Ph.D.
> Director of Confocal&Two-Photon Core
> SUNY Upstate Medical University
> Neuroscience & Physiology Dept
>
> Syracuse, NY
>   >>> Sylvie Le Guyader <[hidden email]> 2/6/2018 6:21 AM >>>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your
> posting.
> *****
>
> Dear all
>
> We have little experience with refurbishing argon lasers. We only have
> 1 refurbished argon and this was done only a couple of months ago.
> Now that we are in need of refurbishing one again, our local vendor
> claims that refurbished argon lasers last a lot less time than new ones
> and often stop working after one year.
> I have search the list archives without finding any comparison of the
> lifetime of refurbished vs new argon lasers.
>
> What is your experience with refurbished argon lasers? Do they last
> less than new argons?
>
> thanks
>
> Med vänlig hälsning / Best regards
>
> Sylvie
>
> @@@@@@@@@@@@@@@@@@@@@@@@
> Sylvie Le Guyader, PhD
> Live Cell Imaging Facility Manager
> Karolinska Institutet- Bionut Dpt
> Hälsovägen 7C,
> Room 7362/7840
> 14157 Huddinge, Sweden
> mobile: [phone]
> LCI website<http://ki.se/en/bionut/welcome-to-the-lci-facility>

--


George McNamara, PhD
Baltimore, MD 21231
[hidden email]
https://www.linkedin.com/in/georgemcnamara
https://works.bepress.com/gmcnamara/75   (may need to use Microsoft Edge or Firefox, rather than Google Chrome)
http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650
http://confocal.jhu.edu

July 2017 Current Protocols article, open access:
UNIT 4.4 Microscopy and Image Analysis
http://onlinelibrary.wiley.com/doi/10.1002/cphg.42/abstract
supporting materials direct link is
http://onlinelibrary.wiley.com/doi/10.1002/cphg.42/full#hg0404-sec-0023
figures at
http://onlinelibrary.wiley.com/doi/10.1002/cphg.42/figures

[Reece, Jeff (NIH/NIDDK) [E]]

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*****

Hi Arvydas,

Try LSM Tech:
http://lsmtech.com/computer-upgrade/

Kind Regards,
Jeff


-----Original Message-----
From: Arvydas Matiukas [mailto:[hidden email]]
Sent: Thursday, February 15, 2018 2:06 PM
To: [hidden email]
Subject: Re: upgrade of 2p system on Axioskop 2 FS mot stand

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hello Microscopists,
 
Please share you thoughts/experience what would be an inexpensive way to upgrade
510 META NLO on the upright Axioskop 2 FS mot stand. i am aiming at the most sensitive available detectors( Idealy 2x GaAsP  for two colors). I would prefer keep the 2p Chameleon laser and the upright stand. By inexpensive plan I assume a fraction of the Thorlabs 2p scope price ($100k).
Fitting into $20k budget would be perfect.
 
Initially I was thinking to reconnect the priginal LSM510 META NLO scanhead to tothe upright stand, however, some people working with 2p setups pointed to me that the sensitivity of such system won't be good enough for the most deep tissue imaging projects. Therefore I am wondering if it is possible to significantly improve sensitivity just by replacing old PMT/NDD detectors, or optics/beampath still be an issue .
 
Thanks,
Arvydas
***************************
 
 


 
 
Arvydas Matiukas, Ph.D.
Director of Confocal&Two-Photon Core
SUNY Upstate Medical University
Neuroscience & Physiology Dept

Syracuse, NY
 >>> Sylvie Le Guyader <[hidden email]> 2/6/2018 6:21 AM >>>
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear all

We have little experience with refurbishing argon lasers. We only have
1 refurbished argon and this was done only a couple of months ago.
Now that we are in need of refurbishing one again, our local vendor claims that refurbished argon lasers last a lot less time than new ones and often stop working after one year.
I have search the list archives without finding any comparison of the lifetime of refurbished vs new argon lasers.

What is your experience with refurbished argon lasers? Do they last less than new argons?

thanks

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Hälsovägen 7C,
Room 7362/7840
14157 Huddinge, Sweden
mobile: [phone]
LCI website<http://ki.se/en/bionut/welcome-to-the-lci-facility>