avoid condensation??

[Richard Cole]

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We use a simple resistive heater on the top, similar to rear window defogger
on automobiles.  This works for days and avoids the need for oils.

 

Cheers

 

Rich

 

 

Richard Cole
Research Scientist V
Director: Advanced Light Microscopy Core Unit
Wadsworth Center

 

Research Assistant Professor
Dept. of Biomedical Sciences
School of Public Health State University of New York


P.O. Box 509 Albany N.Y. 12201-0509
518-474-7048 Phone
518-474-4430 Fax

 

Email  <mailto:[hidden email]> [hidden email]

Website  <http://www.wadsworth.org/cores/alm/index.htm>
www.wadsworth.org/cores/alm/index.htm

 

 



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[Christian Tischer]

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>
>We use these mebranes that have a hydrophobic side to place on top of dishes
instead of lids.  It prevents drying out and has no condensation. I haven't seen
these for 24-well plate, but I'm sure they have them.  we go ours through our
Zeiss applications specialist (no commercial interest)
>
>Yevgeniy
>

Hi Yevgeniy, thanks for the suggestion!
can you do transmission microscopy through those membranes?
Tischi

[Christian Tischer]

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Hi Jim,

wow. thanks!

that sounds very interesting indeed!

what do you mean by "saturating the oil with gas"; i mean what does that
mean in practice; do you put the oil "into gas" before using it???

Tischi



--------------

We cover the medium with a thin layer of mineral oil, which has been
saturated with gas; in our case CO2/O2/N2, at 37 degrees, and don't use
the lid.  Our mineral oil is from Sigma, other mineral oils may extract
ingredients from the medium. When saturated, gas exchange across the oil
is fine, as the absorption coefficients of CO2 and O2 are similar to those
in water (Kubie, JBC, 1927). We perform imaging for up to five days in
this manner, with no loss of medium and no condensation.

Jim Denegre
The Jackson Laboratory

[James Denegre]

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We saturate by leaving dishes of oil in a gassed incubator for at lease on
hour before use.

On 2/22/12 10:53 AM, "Christian Tischer" <[hidden email]> wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Hi Jim,
>
>wow. thanks!
>
>that sounds very interesting indeed!
>
>what do you mean by "saturating the oil with gas"; i mean what does that
>mean in practice; do you put the oil "into gas" before using it???
>
>Tischi
>
>
>
>--------------
>
>We cover the medium with a thin layer of mineral oil, which has been
>saturated with gas; in our case CO2/O2/N2, at 37 degrees, and don't use
>the lid.  Our mineral oil is from Sigma, other mineral oils may extract
>ingredients from the medium. When saturated, gas exchange across the oil
>is fine, as the absorption coefficients of CO2 and O2 are similar to those
>in water (Kubie, JBC, 1927). We perform imaging for up to five days in
>this manner, with no loss of medium and no condensation.
>
>Jim Denegre
>The Jackson Laboratory