big fluorescent beads
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*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi all, Does anyone know of a commerical source of fluorescent beads that are around 25 microns in diameter? We are sorting large cells and need something in that size range to calibrate the instrument. Many thanks, Tobias -- _ ____ __ ____ / \ / / \ / \ \ Tobias I. Baskin / / / / \ \ \ Professor /_ / __ /__ \ \ \__ Biology Department / / / \ \ \ 611 N. Pleasant St. / / / \ \ \ University of Massachusetts / / ___ / \ \__/ \ ____ Amherst, MA, 01003 www.bio.umass.edu/biology/baskin Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243 |
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*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Gary (and anyone following this thread), Thanks. As far I can tell, introgen's beads top our at 15 µm. Well that might be ok, but I was hoping to find 25 µm (it is our average cell diameter). Sorry I didn't make that clear (or perhaps there is a product I missed on their web page amid all of the smaller beads?). As ever Tobias At 1:46 PM -0400 3/27/12, Gary G. Li wrote: >Invitrogen? > >On Tue, Mar 27, 2012 at 1:25 PM, Tobias Baskin ><<mailto:[hidden email]>[hidden email]> >wrote: > >***** >To join, leave or search the confocal microscopy listserv, go to: ><http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >***** > >Hi all, > Does anyone know of a commerical source >of fluorescent beads that are around 25 microns >in diameter? We are sorting large cells and need >something in that size range to calibrate the >instrument. > > Many thanks, > Tobias >-- > _ ____ __ ____ > / \ / / \ / \ \ Tobias I. Baskin > / / / / \ \ \ Professor > /_ / __ /__ \ \ \__ Biology Department > / / / \ \ \ 611 N. Pleasant St. > / / / \ \ \ > University of Massachusetts >/ / ___ / \ \__/ \ ____ Amherst, MA, 01003 ><http://www.bio.umass.edu/biology/baskin>www.bio.umass.edu/biology/baskin >Voice: <tel:413%20-%20545%20-%201533>413 - 545 - >1533 Fax: <tel:413%20-%20545%20-%203243>413 - >545 - 3243 |
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In reply to this post by Tobias Baskin
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** You may check spherotech. They appear to have particles up to 35 µm. Catalog link below. http://spherotech.com/five_up.htm Thanks Abhijit Deb Roy Biomedical Sciences Program University of Connecticut Health Centre, Farmington On Tue, Mar 27, 2012 at 1:58 PM, Tobias Baskin <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > ***** > > Gary (and anyone following this thread), > Thanks. As far I can tell, introgen's beads top our > at 15 µm. Well that might be ok, but I was hoping to find 25 µm (it is our > average cell diameter). Sorry I didn't make that clear (or perhaps there is > a product I missed on their web page amid all of the smaller beads?). > > As ever > Tobias > > At 1:46 PM -0400 3/27/12, Gary G. Li wrote: > >> Invitrogen? >> >> >> On Tue, Mar 27, 2012 at 1:25 PM, Tobias Baskin <<mailto: >> [hidden email]>**[hidden email] <[hidden email]>> >> wrote: >> >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> <http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> >> >http:**//lists.umn.edu/cgi-bin/wa?A0=**confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> >> >> ***** >> >> Hi all, >> Does anyone know of a commerical source of fluorescent beads that >> are around 25 microns in diameter? We are sorting large cells and need >> something in that size range to calibrate the instrument. >> >> Many thanks, >> Tobias >> -- >> _ ____ __ ____ >> / \ / / \ / \ \ Tobias I. Baskin >> / / / / \ \ \ Professor >> /_ / __ /__ \ \ \__ Biology Department >> / / / \ \ \ 611 N. Pleasant St. >> / / / \ \ \ University of Massachusetts >> / / ___ / \ \__/ \ ____ Amherst, MA, 01003 >> <http://www.bio.umass.edu/**biology/baskin<http://www.bio.umass.edu/biology/baskin> >> >www.bio.umass.**edu/biology/baskin<http://www.bio.umass.edu/biology/baskin> >> Voice: <tel:413%20-%20545%20-%201533>413 - 545 - 1533 Fax: >> <tel:413%20-%20545%20-%203243>413 - 545 - 3243 >> > -- |
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In reply to this post by Tobias Baskin
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Polysciences has fluorescent polystyrene in that size range: http://www.polysciences.com/Catalog/Department/81/categoryId__338/ Kisker (Discover science) has 10micron fluorescent silica and 20 micron biotinylated that should be easy to label with fluorescent avidin. http://www.microparticles-nanospheres.com/micro_silicia.htm Bang has 10-25 micron fluorescent http://www.bangslabs.com/products/fluorescent_microspheres On Mar 27, 2012, at 6:25 PM, Tobias Baskin wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi all, > Does anyone know of a commerical source of fluorescent beads > that are around 25 microns in diameter? We are sorting large cells > and need something in that size range to calibrate the instrument. > > Many thanks, > Tobias > -- > _ ____ __ ____ > / \ / / \ / \ \ Tobias I. Baskin > / / / / \ \ \ Professor > /_ / __ /__ \ \ \__ Biology Department > / / / \ \ \ 611 N. Pleasant St. > / / / \ \ \ University of > Massachusetts > / / ___ / \ \__/ \ ____ Amherst, MA, 01003 > www.bio.umass.edu/biology/baskin > Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243 Visiting Professor David Knecht Beatson Institute for Cancer Research University of Glasgow Switchback Road, Bearsden Glasgow Scotland G61 1BD UK Visiting Professor David Knecht Beatson Institute for Cancer Research University of Glasgow Switchback Road, Bearsden Glasgow Scotland G61 1BD UK |
 
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Phillips, Thomas E. |
Re: big fluorescent beads
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In reply to this post by Tobias Baskin
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Check out http://bangslabs.com/ - they may have what you want. tom Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) [hidden email] http://www.biology.missouri.edu/faculty/phillips.html http://www.biotech.missouri.edu/mcc/ -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Tobias Baskin Sent: Tuesday, March 27, 2012 12:58 PM To: [hidden email] Subject: Re: big fluorescent beads ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Gary (and anyone following this thread), Thanks. As far I can tell, introgen's beads top our at 15 µm. Well that might be ok, but I was hoping to find 25 µm (it is our average cell diameter). Sorry I didn't make that clear (or perhaps there is a product I missed on their web page amid all of the smaller beads?). As ever Tobias At 1:46 PM -0400 3/27/12, Gary G. Li wrote: >Invitrogen? > >On Tue, Mar 27, 2012 at 1:25 PM, Tobias Baskin ><<mailto:[hidden email]>[hidden email]> >wrote: > >***** >To join, leave or search the confocal microscopy listserv, go to: ><http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>http://lists.umn >.edu/cgi-bin/wa?A0=confocalmicroscopy >***** > >Hi all, > Does anyone know of a commerical source of fluorescent beads >that are around 25 microns in diameter? We are sorting large cells and >need something in that size range to calibrate the instrument. > > Many thanks, > Tobias >-- > _ ____ __ ____ > / \ / / \ / \ \ Tobias I. Baskin > / / / / \ \ \ Professor > /_ / __ /__ \ \ \__ Biology Department > / / / \ \ \ 611 N. Pleasant St. > / / / \ \ \ > University of Massachusetts >/ / ___ / \ \__/ \ ____ Amherst, MA, 01003 ><http://www.bio.umass.edu/biology/baskin>www.bio.umass.edu/biology/bask >in >Voice: <tel:413%20-%20545%20-%201533>413 - 545 - >1533 Fax: <tel:413%20-%20545%20-%203243>413 - >545 - 3243 |
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In reply to this post by Knecht, David
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** As David has said, do check out Polysciences: http://www.polysciences.com/Catalog/40/categoryId__4 They used to manufacture beads to your specification if required, and I don't think that has changed or that it was that expensive - but it's been many years since I last did it. Regards Keith --------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom. Telephone: +44 (0)1865 287568 Email: [hidden email] Web-pages: http://www.well.ox.ac.uk/molecular-cytogenetics-and-microscopy -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of David Knecht Sent: 27 March 2012 19:10 To: [hidden email] Subject: Re: big fluorescent beads ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Polysciences has fluorescent polystyrene in that size range: http://www.polysciences.com/Catalog/Department/81/categoryId__338/ Kisker (Discover science) has 10micron fluorescent silica and 20 micron biotinylated that should be easy to label with fluorescent avidin. http://www.microparticles-nanospheres.com/micro_silicia.htm Bang has 10-25 micron fluorescent http://www.bangslabs.com/products/fluorescent_microspheres On Mar 27, 2012, at 6:25 PM, Tobias Baskin wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi all, > Does anyone know of a commerical source of fluorescent beads > that are around 25 microns in diameter? We are sorting large cells > and need something in that size range to calibrate the instrument. > > Many thanks, > Tobias > -- > _ ____ __ ____ > / \ / / \ / \ \ Tobias I. Baskin > / / / / \ \ \ Professor > /_ / __ /__ \ \ \__ Biology Department > / / / \ \ \ 611 N. Pleasant St. > / / / \ \ \ University of > Massachusetts > / / ___ / \ \__/ \ ____ Amherst, MA, 01003 > www.bio.umass.edu/biology/baskin > Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243 Visiting Professor David Knecht Beatson Institute for Cancer Research University of Glasgow Switchback Road, Bearsden Glasgow Scotland G61 1BD UK Visiting Professor David Knecht Beatson Institute for Cancer Research University of Glasgow Switchback Road, Bearsden Glasgow Scotland G61 1BD UK |
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John Oreopoulos |
Small multi-color fluorescent beads (was "big fluorescent beads")
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Somewhat related to this last posting, can anyone recommend a source of small (50 nm diameter or less) multi-color (at least two distinct emission spectra) fluorescent microspheres? Commercial vendors may also contact me directly offline. Thank you. John Oreopoulos Research Assistant Spectral Applied Research Richmond Hill, Ontario Canada www.spectral.ca On 2012-03-28, at 4:59 AM, Keith Morris wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > As David has said, do check out Polysciences: > > http://www.polysciences.com/Catalog/40/categoryId__4 > > They used to manufacture beads to your specification if required, and I > don't think that has changed or that it was that expensive - but it's been > many years since I last did it. > > Regards > > Keith > > --------------------------------------------------------------------------- > Dr Keith J. Morris, > Molecular Cytogenetics and Microscopy Core, > Laboratory 00/069 and 00/070, > The Wellcome Trust Centre for Human Genetics, > Roosevelt Drive, > Oxford OX3 7BN, > United Kingdom. > > Telephone: +44 (0)1865 287568 > Email: [hidden email] > Web-pages: http://www.well.ox.ac.uk/molecular-cytogenetics-and-microscopy > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On > Behalf Of David Knecht > Sent: 27 March 2012 19:10 > To: [hidden email] > Subject: Re: big fluorescent beads > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Polysciences has fluorescent polystyrene in that size range: > http://www.polysciences.com/Catalog/Department/81/categoryId__338/ > > Kisker (Discover science) has 10micron fluorescent silica and 20 micron > biotinylated that should be easy to label with fluorescent avidin. > http://www.microparticles-nanospheres.com/micro_silicia.htm > > Bang has 10-25 micron fluorescent > http://www.bangslabs.com/products/fluorescent_microspheres > > > On Mar 27, 2012, at 6:25 PM, Tobias Baskin wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Hi all, >> Does anyone know of a commerical source of fluorescent beads >> that are around 25 microns in diameter? We are sorting large cells >> and need something in that size range to calibrate the instrument. >> >> Many thanks, >> Tobias >> -- >> _ ____ __ ____ >> / \ / / \ / \ \ Tobias I. Baskin >> / / / / \ \ \ Professor >> /_ / __ /__ \ \ \__ Biology Department >> / / / \ \ \ 611 N. Pleasant St. >> / / / \ \ \ University of >> Massachusetts >> / / ___ / \ \__/ \ ____ Amherst, MA, 01003 >> www.bio.umass.edu/biology/baskin >> Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243 > > Visiting Professor David Knecht > Beatson Institute for Cancer Research > University of Glasgow > Switchback Road, Bearsden > Glasgow Scotland G61 1BD > UK > > > > > > > > Visiting Professor David Knecht > Beatson Institute for Cancer Research > University of Glasgow > Switchback Road, Bearsden > Glasgow Scotland G61 1BD > UK |
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