cmos camera: appearance of boxes/bubbles over images

[Laurel Ballantyne]

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Images can be seen here,
https://imgur.com/a/ff7aePj

Has anyone seen/experienced this issue before?  Minutes into using the camera, these strange "boxes" appear in the center of the image and spread to cover the whole field, eventually turning into "bubbles".  These "boxes" are the same size no matter the magnification used.  Any help/advice is greatly appreciated!

[Oshel, Philip Eugene]

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Do you see these features after some period of observing with the binocs (your eyes) and not using the camera? If so, I'd suspect your mounting medium.

Phil
-------------
Philip Oshel    
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> on behalf of Laurel Ballantyne <[hidden email]>
Reply-To: Confocal Microscopy List <[hidden email]>
Date: Friday,  10January, 2020 at 10:55
To: "[hidden email]" <[hidden email]>
Subject: cmos camera: appearance of boxes/bubbles over images

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    Images can be seen here,
    https://imgur.com/a/ff7aePj
   
    Has anyone seen/experienced this issue before?  Minutes into using the camera, these strange "boxes" appear in the center of the image and spread to cover the whole field, eventually turning into "bubbles".  These "boxes" are the same size no matter the magnification used.  Any help/advice is greatly appreciated!
   

[weber]

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I had this once. There was moisture between the sensor and the protective glass.
Hamamatsu have changed the camera.
Best Regards

[Michael Delannoy]

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Hello,
I have never seen boxes before, but if the artifact appears under the beam to grow then the section is probably still wet.  Otherwise perhaps a salt contamination.  Good luck.

Sincerely,
Michael Delannoy

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Laurel Ballantyne
Sent: Friday, January 10, 2020 10:45 AM
To: [hidden email]
Subject: cmos camera: appearance of boxes/bubbles over images

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Images can be seen here,
https://imgur.com/a/ff7aePj

Has anyone seen/experienced this issue before?  Minutes into using the camera, these strange "boxes" appear in the center of the image and spread to cover the whole field, eventually turning into "bubbles".  These "boxes" are the same size no matter the magnification used.  Any help/advice is greatly appreciated!

[Thews, Elmar]

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Moin Moin Laurel,

definitely broken seal in the camera with condensation on the inner surface of the protective window. If you leave the camera on for a few days it could vanish.

Tschüß
Elmar
53°35'15.55'' N; 9°54'21.35'' E, 25m above NHN
-
Dr. Elmar Thews | Principal Scientist, DAS
PerkinElmer | For the Better
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-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Laurel Ballantyne
Sent: Freitag, 10. Januar 2020 16:45
To: [hidden email]
Subject: [External] cmos camera: appearance of boxes/bubbles over images


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Images can be seen here,
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Has anyone seen/experienced this issue before?  Minutes into using the camera, these strange "boxes" appear in the center of the image and spread to cover the whole field, eventually turning into "bubbles".  These "boxes" are the same size no matter the magnification used.  Any help/advice is greatly appreciated!

PerkinElmer Cellular Technologies Germany GmbH
Sitz Hamburg
Amtsgericht Hamburg, HRB 101440
Geschäftsführer: Dr. Jürgen Müller

[Tanneke den Blaauwen]

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Test whether you see it also if you only have an object glass. We had the same with an hamamatsu camera and got a replacement. Condense in the camera. For the rest the camara is very good : >)

Tanneke


On 10 Jan 2020, at 17:05, Oshel, Philip Eugene <[hidden email]<mailto:[hidden email]>> wrote:

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Do you see these features after some period of observing with the binocs (your eyes) and not using the camera? If so, I'd suspect your mounting medium.

Phil
-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> on behalf of Laurel Ballantyne <[hidden email]>
Reply-To: Confocal Microscopy List <[hidden email]>
Date: Friday,  10January, 2020 at 10:55
To: "[hidden email]" <[hidden email]>
Subject: cmos camera: appearance of boxes/bubbles over images

   *****
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   Images can be seen here,
   https://imgur.com/a/ff7aePj

   Has anyone seen/experienced this issue before?  Minutes into using the camera, these strange "boxes" appear in the center of the image and spread to cover the whole field, eventually turning into "bubbles".  These "boxes" are the same size no matter the magnification used.  Any help/advice is greatly appreciated!



Guardare meravigliarsi et tornar a guardare

Tanneke den Blaauwen
Assoc prof Bacterial Cell Biology

Visiting address:
SILS, UvA
Science Park 904, C3.108
1098 XH Amsterdam, NL
[hidden email]<mailto:[hidden email]>
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Mail address:
T. den Blaauwen
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SILS
PO BOX 94215
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The Netherlands

[Brian Kain]

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If you rotate the camera while viewing live, do the bubbles move with the image?  If not, then the bubbles are on the camera, and likely condensation from a failed seal within the camera.  Best to contact your local camera or microscope rep and review for warranty replacement.
Best,
Brian

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Michael Delannoy
Sent: Friday, January 10, 2020 11:10 AM
To: [hidden email]
Subject: Re: cmos camera: appearance of boxes/bubbles over images

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Hello,
I have never seen boxes before, but if the artifact appears under the beam to grow then the section is probably still wet.  Otherwise perhaps a salt contamination.  Good luck.

Sincerely,
Michael Delannoy

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Laurel Ballantyne
Sent: Friday, January 10, 2020 10:45 AM
To: [hidden email]
Subject: cmos camera: appearance of boxes/bubbles over images

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Images can be seen here,
https://urldefense.proofpoint.com/v2/url?u=https-3A__imgur.com_a_ff7aePj&d=DwIGaQ&c=7WMbxJudsInepJ54aTu5FB4kE34qljjw4wOb_YlLTpQ&r=gIFsZfJbmBb0FkHwBLdl_lx5F1ErV-NmoknCO-hDrUw&m=wUz-PScfcYnQlmJKViUrG8EMeNMYxj4kYDLPzDXq8E0&s=fuLGaTLg3-tm9Cv-o53bE_3bZrx_TVqJ474o_cTXfWk&e= 

Has anyone seen/experienced this issue before?  Minutes into using the camera, these strange "boxes" appear in the center of the image and spread to cover the whole field, eventually turning into "bubbles".  These "boxes" are the same size no matter the magnification used.  Any help/advice is greatly appreciated!

[Laurel Ballantyne]

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They're only there through the camera, not when looking through the eyepieces.

[Laurel Ballantyne]

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Thanks for the input, though quite sad.

[Ho-chun LAI]

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We did experienced once with a Zyla camera.
No the condensation won't go away and would need a factory refurbishing.
We then put a dehumidifier right next to it to prevent it from happening again.

Best regards,
John

________________________________
寄件者: Confocal Microscopy List <[hidden email]> 代表 Thews, Elmar <[hidden email]>
寄件日期: 2020年1月11日 0:12
收件者: [hidden email] <[hidden email]>
主旨: Re: [External] cmos camera: appearance of boxes/bubbles over images

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Moin Moin Laurel,

definitely broken seal in the camera with condensation on the inner surface of the protective window. If you leave the camera on for a few days it could vanish.

Tschüß
Elmar
53°35'15.55'' N; 9°54'21.35'' E, 25m above NHN
-
Dr. Elmar Thews | Principal Scientist, DAS
PerkinElmer | For the Better
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-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Laurel Ballantyne
Sent: Freitag, 10. Januar 2020 16:45
To: [hidden email]
Subject: [External] cmos camera: appearance of boxes/bubbles over images


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Images can be seen here,
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Has anyone seen/experienced this issue before?  Minutes into using the camera, these strange "boxes" appear in the center of the image and spread to cover the whole field, eventually turning into "bubbles".  These "boxes" are the same size no matter the magnification used.  Any help/advice is greatly appreciated!

PerkinElmer Cellular Technologies Germany GmbH
Sitz Hamburg
Amtsgericht Hamburg, HRB 101440
Geschäftsführer: Dr. Jürgen Müller

[Cammer, Michael]

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Back in the day, we had to periodically hook up our Princeton Instruments camera to a vacuum pump.

More recently, a seal on a sCMOS broke.  One picture of the condensation that built up as the camera cooled:  https://www.flickr.com/photos/mcammer/34501637964/ 

The camera company fixed it.

Cheers-
Michael C.

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Ho-chun LAI
Sent: Friday, January 10, 2020 11:26 AM
To: [hidden email]
Subject: 回覆: [External] cmos camera: appearance of boxes/bubbles over images

[EXTERNAL]

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We did experienced once with a Zyla camera.
No the condensation won't go away and would need a factory refurbishing.
We then put a dehumidifier right next to it to prevent it from happening again.

Best regards,
John

________________________________
寄件者: Confocal Microscopy List <[hidden email]> 代表 Thews, Elmar <[hidden email]>
寄件日期: 2020年1月11日 0:12
收件者: [hidden email] <[hidden email]>
主旨: Re: [External] cmos camera: appearance of boxes/bubbles over images

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Moin Moin Laurel,

definitely broken seal in the camera with condensation on the inner surface of the protective window. If you leave the camera on for a few days it could vanish.

Tschüß
Elmar
53°35'15.55'' N; 9°54'21.35'' E, 25m above NHN
-
Dr. Elmar Thews | Principal Scientist, DAS PerkinElmer | For the Better [hidden email]
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-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Laurel Ballantyne
Sent: Freitag, 10. Januar 2020 16:45
To: [hidden email]
Subject: [External] cmos camera: appearance of boxes/bubbles over images


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Has anyone seen/experienced this issue before?  Minutes into using the camera, these strange "boxes" appear in the center of the image and spread to cover the whole field, eventually turning into "bubbles".  These "boxes" are the same size no matter the magnification used.  Any help/advice is greatly appreciated!

PerkinElmer Cellular Technologies Germany GmbH Sitz Hamburg Amtsgericht Hamburg, HRB 101440
Geschäftsführer: Dr. Jürgen Müller

[Laurel Ballantyne]

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Hi Brian,

When I rotate the camera, the squares/bubbles don't move.  Thanks for the info!

[Laurel Ballantyne]

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Thanks so much, everyone, for all the info!  We'll look into sending the camera back for repair.