concetric circles

[nicoletta fucà]

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Hi all,

I got a problem with my confocal microscope.
I see in the image some concentric circles.
Any advices to work this problem out?

thank you in advance

Nicoletta

[Cromey, Douglas W - (dcromey)]

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Newton rings from imaging too close to the coverslip (reflections)?
Doug

^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Douglas W. Cromey, M.S. - Associate Scientific Investigator
Dept. of Cellular & Molecular Medicine, University of Arizona
1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA

office:  AHSC 4212         email: [hidden email]
voice:  520-626-2824       fax:  [phone]

http://swehsc.pharmacy.arizona.edu/exppath/
Home of: "Microscopy and Imaging Resources on the WWW"

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Nicoletta Fucà
Sent: Wednesday, July 18, 2012 3:12 AM
To: [hidden email]
Subject: concetric circles

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Hi all,

I got a problem with my confocal microscope.
I see in the image some concentric circles.
Any advices to work this problem out?

thank you in advance

Nicoletta

[Glen MacDonald-2]

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Dear Nicoletta,
Does this occur with all lenses or predominately low magnification lenses, all wavelengths or more noticeable with short wavelengths?  As Doug said, could be coverslip.  Other possibilities are a DIC analyzer in the light path, double coverslips (it happens), choice of mounting medium or an incorrect or deteriorating dichroic or filter allowing  laser reflections to leak into the detector.  

Glen
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
[phone]
[hidden email]








On Jul 18, 2012, at 3:12 AM, Nicoletta Fucà  wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi all,
>
> I got a problem with my confocal microscope.
> I see in the image some concentric circles.
> Any advices to work this problem out?
>
> thank you in advance
>
> Nicoletta

[JOEL B. SHEFFIELD]

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This is interesting. I agree that it might be Newton's rings.   On our
Leica, SP!, which has spectral filters, we often see the rings if we set
the wavelength detector too close to the exciting wavelength.

Joel


On Wed, Jul 18, 2012 at 10:26 AM, Cromey, Douglas W - (dcromey) <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Newton rings from imaging too close to the coverslip (reflections)?
> Doug
>
> ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
> Douglas W. Cromey, M.S. - Associate Scientific Investigator
> Dept. of Cellular & Molecular Medicine, University of Arizona
> 1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA
>
> office:  AHSC 4212         email: [hidden email]
> voice:  520-626-2824       fax:  [phone]
>
> http://swehsc.pharmacy.arizona.edu/exppath/
> Home of: "Microscopy and Imaging Resources on the WWW"
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Nicoletta FucÃ
> Sent: Wednesday, July 18, 2012 3:12 AM
> To: [hidden email]
> Subject: concetric circles
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi all,
>
> I got a problem with my confocal microscope.
> I see in the image some concentric circles.
> Any advices to work this problem out?
>
> thank you in advance
>
> Nicoletta
>

--


Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL:  http://astro.temple.edu/~jbs

[nicoletta fucà]

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I have a Nikon C1si Spectral Confocal system on a Nikon TE2000E inverted
motorised microscope, I used a 60x lens and the 488 filter, placed on a Melles
Griot Ar laser 457-514 nm. It didn't happen with other channels but it happened
also with the 40x lens.
I saw the rings just on the upper left side of the image. The weird thing is that it
happened for two consecutive days, and this morning it didn't happen anymore.
That is the setting I usually use. I can say that it happened after I and a collegue
of mine alligned the laser.