confocal for a teaching lab
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello, I am helping to prepare a set of labs for a graduate course on biological light microscopy. For a few of the labs, we are interested in constructing a confocal microscope, to show students how it works, and to use in imaging cells. Specifically, we're interested in the nipkow disk-scanning type (though we are still considering some designs using conventional scanning with galvos). I am wondering if anyone with experience doing this has any words of advice? Also, I would be interested to know if anyone has or knows of an old or broken confocal that we might be able to purchase at a bargain either use for parts or repair. Thanks in advance, Matt Nicholas MSTP III Albert Einstein College of Medicine |
 
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Paul Herzmark |
Fwd: confocal for a teaching lab
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Mathew,
The book Fundamentals of Light Microscopy and electronic imaging by Douglas B. Murphy has a nice experimental bench-top confocal on page 212 But it doesn't involve either scan mirrors or a spinning disk. -- Paul Herzmark Specialist [hidden email] Department of Molecular and Cell Biology 479 Life Science Addition University of California, Berkeley Berkeley, CA 94720-3200 (510) 643-9603 (510) 643-9500 fax ---------- Forwarded message ---------- From: Matthew Nicholas <[hidden email]> Date: Fri, Aug 8, 2008 at 9:40 AM Subject: confocal for a teaching lab To: [hidden email] Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello, I am helping to prepare a set of labs for a graduate course on biological light microscopy. For a few of the labs, we are interested in constructing a confocal microscope, to show students how it works, and to use in imaging cells. Specifically, we're interested in the nipkow disk-scanning type (though we are still considering some designs using conventional scanning with galvos). I am wondering if anyone with experience doing this has any words of advice? Also, I would be interested to know if anyone has or knows of an old or broken confocal that we might be able to purchase at a bargain either use for parts or repair. Thanks in advance, Matt Nicholas MSTP III Albert Einstein College of Medicine |
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In reply to this post by Matthew Nicholas
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
You'd really have to buy the Nipkow disk - pretty hard
to make.
The 'easy' way to set up a confocal microscope on an
optical table
would be to use stage scanning - either with stepper motors
or
by
using the Colin Sheppard technique of using the voice
coils
from small speakers.
Guy Optical Imaging Techniques in Cell Biology From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Paul Herzmark Sent: Saturday, 9 August 2008 3:22 AM To: [hidden email] Subject: Fwd: confocal for a teaching lab Mathew, The book Fundamentals of Light Microscopy and electronic imaging by Douglas B. Murphy has a nice experimental bench-top confocal on page 212 But it doesn't involve either scan mirrors or a spinning disk. -- Paul Herzmark Specialist [hidden email] Department of Molecular and Cell Biology 479 Life Science Addition University of California, Berkeley Berkeley, CA 94720-3200 (510) 643-9603 (510) 643-9500 fax ---------- Forwarded message ---------- From: Matthew Nicholas <[hidden email]> Date: Fri, Aug 8, 2008 at 9:40 AM Subject: confocal for a teaching lab To: [hidden email] Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello, I am helping to prepare a set of labs for a graduate course on biological light microscopy. For a few of the labs, we are interested in constructing a confocal microscope, to show students how it works, and to use in imaging cells. Specifically, we're interested in the nipkow disk-scanning type (though we are still considering some designs using conventional scanning with galvos). I am wondering if anyone with experience doing this has any words of advice? Also, I would be interested to know if anyone has or knows of an old or broken confocal that we might be able to purchase at a bargain either use for parts or repair. Thanks in advance, Matt Nicholas MSTP III Albert Einstein College of Medicine No virus found in this incoming message. No virus found in this outgoing message. |
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