correcting SA by changing tube length

[Stanislav Vitha-2]

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Hallo,
is there a simple formula or a web calculator (or Excel sheet) to calculate how much I need to move the camera to correct for spherical aberration, e.g. when using a 20x/0.7 dry,  coverslip-corrected objective on a sample without a coverslip.
I  used the calculation a while back and cannot find my notes.
Re-reading the papers by Sheppard (e.g., Sheppard, C.J.R. (2000) Confocal imaging through weakly aberrating media. Applied optics, 39, 6366-6368) no longer makes as much sense as it used to.

Thanks in advance!

Best regards,

Stan Vitha
Microcopy and Imaging Center
Texas A&M University
College Station, TX

[Dan Focht]

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Stan


Infiniti photo-optical makes a BFP SA corrector.
http://www.infinity-usa.com/

I used it once 15 years ago.
I don't know what they are doing now, but it worked back then.


Dan
 

On Jan 26, 2017, at 1:05 PM, Stanislav Vitha wrote:

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Hallo,
is there a simple formula or a web calculator (or Excel sheet) to calculate how much I need to move the camera to correct for spherical aberration, e.g. when using a 20x/0.7 dry,  coverslip-corrected objective on a sample without a coverslip.
I  used the calculation a while back and cannot find my notes.
Re-reading the papers by Sheppard (e.g., Sheppard, C.J.R. (2000) Confocal imaging through weakly aberrating media. Applied optics, 39, 6366-6368) no longer makes as much sense as it used to.

Thanks in advance!

Best regards,

Stan Vitha
Microcopy and Imaging Center
Texas A&M University
College Station, TX

Dan Focht
Bioptechs, Inc.
3560 Beck Rd.
Butler, PA 16002
www.bioptechs.com
P: [phone]
F: [phone]
[hidden email]

[samuel connell]

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***Commercial Reply***

Hi Stan,

3i makes a unique device for motorized high-speed spherical aberration
correction.

https://www.intelligent-imaging.com/components#msac

Let me know if you have any questions or more curiosity.

Best Regards,
-
Sam

Samuel Connell
Senior Applications Scientist

Intelligent Imaging Innovations (3i)
3509 Ringsby Court
Denver, CO  80216  USA
1-720-437-6926
[hidden email]
www.intelligent-imaging.com

On Thu, Jan 26, 2017 at 10:05 AM, Stanislav Vitha <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hallo,
> is there a simple formula or a web calculator (or Excel sheet) to
> calculate how much I need to move the camera to correct for spherical
> aberration, e.g. when using a 20x/0.7 dry,  coverslip-corrected objective
> on a sample without a coverslip.
> I  used the calculation a while back and cannot find my notes.
> Re-reading the papers by Sheppard (e.g., Sheppard, C.J.R. (2000) Confocal
> imaging through weakly aberrating media. Applied optics, 39, 6366-6368) no
> longer makes as much sense as it used to.
>
> Thanks in advance!
>
> Best regards,
>
> Stan Vitha
> Microcopy and Imaging Center
> Texas A&M University
> College Station, TX
>

[Stanislav Vitha-2]

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FYI,
I have found my old notes regarding the calculation. It looks too simple to be correct (I never tested it), but it sort of makes sense.
It may be a good starting point for experimental tuning:

"You could extend the mechanical tube length of the microscope (position the camera system further away),  this introduces spherical aberration that is opposite to that caused by a missing coverslip.
The amount of required adjustment depends on the objective magnification(squared). The calculations suggest that for a 40x objective designed for 0.17 mm coverglass and used without, you would have to extend the mechanical tube length by 108 mm, which is impractical. With a 20x objective, the required adjustment would be a more reasonable 27 mm.

deltaT = deltaD * 0.4 * M^2

where deltaT = tube length adjustment
deltaD = difference in coverglass thickness between the design and actual thickness  (0.17 mm)
M = objective magnification
 

Best Regards,

Stan Vitha
Microscopy and Imaging Center
Texas A&M University