disinfecting a microscope

[Cromey, Douglas W - (dcromey)]

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What is an appropriate method for disinfecting a microscope used for live cell imaging (or in a BSL2 facility)? 10% bleach is too strong, so what do people use? Thinking of core facility users (who can be poor at following directions), what is a relatively fool-proof way to decontaminate and not damage motorized stages, and other delicate parts?

If you have posted protocols, can you send the links?  Do you have live cell imaging policies for your facility that you would be willing to share?

Doug

------------------------------------------------------------------------------------------
Douglas W. Cromey, M.S. - Associate Scientific Investigator
Dept. of Cellular & Molecular Medicine, University of Arizona
1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA

office:  UACC 0914A       email: [hidden email]<mailto:[hidden email]>
voice:  520-626-2824       fax:  [phone]

http://swehsc.pharmacy.arizona.edu/micro
Home of: "Microscopy and Imaging Resources on the WWW"

UA Microscopy Alliance -  http://microscopy.arizona.edu<http://microscopy.arizona.edu/>

[Anil Poudel]

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Hello there,
 
I am new to fluorescence microscopy and trying some F-actin staining kit (Phalloidin 555 from Invitrogen) but having hard time to get very clean filamentous picture and also unable to get the image on screen as it appears through eyepiece. I am using Olympus IX71 with qclick monochorome camera and cellsens software. Any suggestion would for optimization is highly appreciated. Thanks in advance.

Anil
________________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of Cromey, Douglas W - (dcromey) <[hidden email]>
Sent: Wednesday, December 16, 2015 2:01 PM
To: [hidden email]
Subject: disinfecting a microscope

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

What is an appropriate method for disinfecting a microscope used for live cell imaging (or in a BSL2 facility)? 10% bleach is too strong, so what do people use? Thinking of core facility users (who can be poor at following directions), what is a relatively fool-proof way to decontaminate and not damage motorized stages, and other delicate parts?

If you have posted protocols, can you send the links?  Do you have live cell imaging policies for your facility that you would be willing to share?

Doug

------------------------------------------------------------------------------------------
Douglas W. Cromey, M.S. - Associate Scientific Investigator
Dept. of Cellular & Molecular Medicine, University of Arizona
1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA

office:  UACC 0914A       email: [hidden email]<mailto:[hidden email]>
voice:  520-626-2824       fax:  [phone]

http://swehsc.pharmacy.arizona.edu/micro
Home of: "Microscopy and Imaging Resources on the WWW"

UA Microscopy Alliance -  http://microscopy.arizona.edu<http://microscopy.arizona.edu/>

[Periasamy, Ammasi (ap3t)]

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Doug
This is what we use approved by our University Biosafety
"Cavi Cide" you can spray on the microscopy stage and the desk area and wipe it.
Hope this helps.
Ammasi
Dr. Ammasi Periasamy
Professor & Center Director
http://www.kcci.virginia.edu/people/profile/ap3t
Phone: [phone] or 982-4869
Fax: [phone]
E-mail: [hidden email]
Office Location
W.M. Keck Center for Cellular Imaging
Physical and Life Sciences Building, (005)
At the intersection of Geldard dr and White head Rd.,
Mailing or Shipping Address:
W.M. Keck Center for Cellular Imaging (PLSB 005)
University of Virginia
Biology, Gilmer Hall, 409 McCormick Rd.
Charlottesville, VA 22904, USA
FRET/FLIM Workshop-March 7-11, 2016: http://www.kcci.virginia.edu/workshop 


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Cromey, Douglas W - (dcromey)
Sent: Wednesday, December 16, 2015 2:02 PM
To: [hidden email]
Subject: disinfecting a microscope

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

What is an appropriate method for disinfecting a microscope used for live cell imaging (or in a BSL2 facility)? 10% bleach is too strong, so what do people use? Thinking of core facility users (who can be poor at following directions), what is a relatively fool-proof way to decontaminate and not damage motorized stages, and other delicate parts?

If you have posted protocols, can you send the links?  Do you have live cell imaging policies for your facility that you would be willing to share?

Doug

------------------------------------------------------------------------------------------
Douglas W. Cromey, M.S. - Associate Scientific Investigator Dept. of Cellular & Molecular Medicine, University of Arizona
1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA

office:  UACC 0914A       email: [hidden email]<mailto:[hidden email]>
voice:  520-626-2824       fax:  [phone]

http://swehsc.pharmacy.arizona.edu/micro
Home of: "Microscopy and Imaging Resources on the WWW"

UA Microscopy Alliance -  http://microscopy.arizona.edu<http://microscopy.arizona.edu/>

[George McNamara]

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Hi Anil,

Eyepieces ... get the camera image in focus, then adjust the eyepieces
to match YOUR eyes. the IX71 should have adjustable eyepieces. If you
wear glasses (or contacts) adjust either on (or off) and look that way
consistently. If you can't get the eyepieces to match the camera, either
the camera was installed incorrectly or you need to new glasses.

Staining ... don't overstain, wash enough to get rid of background, use
"best" mounting medium -- probably not PBS. that said, a lot simpler if
you start with PBS.

Don't spend the rest of your life making coverglass-slide preparations.
It's an inverted microscope: use imaging dishes. I mostly use

http://glass-bottom-dishes.com/gbcustomerpriceweb.pdf
P35G-1.5-20-C
(20 mm diameter glass imaging area, #1.5 coverglass).
If comparing live cell cultures, I often use the Greiner Bio-One
CellView quadrant dishes ("4 compartments"),
https://shop.gbo.com/en/row/articles/catalogue/articles/0110_0110_0040_0020/
(prolonged live cultures, i usually overlay Sigma-Aldrich M8410 mineral
oil, since my PECON litte incubator thing allows the dishes to dry out)
Nikon also sells "Hi-Q" quadrant dishes, with a funky upper plastic
thingy to (try to) reduce the meniscus effect on transmitted light
imaging (one of many reasons I pay more attention to the
epi-fluorescence than the transmitted light channel).

For starting, I suggest 20x/0.75 (20x/0.70 NA if Olympus did not sell
you one with that extra 0.05), and change fields of view pretty often to
avoid photobleaching.

I might - or not - have useful tips in the MPMicro download available at
http://works.bepress.com/gmcnamara/2/
(one tip i need to mention here; please DO NOT HIT THE PRINT BUTTON -
thanks).
I haven't updated MPMicro in a while, so this part may be out of date
(that is, more than 50% of URLs dead links), my favorite section is
"Accessorize". The section I still use the most is the one with the
table of refractive indices.

For any listservites in (or near) Houston: Marty Chalfie is speaking at
MDACC on Jan 7 and 8 (2016). Should be fun.

George
p.s. if colleagues want a look, simpler to use the monitor and focus
with live camera, than taking turns readjusting the eyepieces all the time.
For "wet mounts" with fixed cells i tend to use 80% glycerol:20% PBS, pH
7.4. Work ok for most fluorophores.

On 12/16/2015 1:07 PM, Anil Poudel wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello there,
>  
> I am new to fluorescence microscopy and trying some F-actin staining kit (Phalloidin 555 from Invitrogen) but having hard time to get very clean filamentous picture and also unable to get the image on screen as it appears through eyepiece. I am using Olympus IX71 with qclick monochorome camera and cellsens software. Any suggestion would for optimization is highly appreciated. Thanks in advance.
>
> Anil
> ________________________________________
> From: Confocal Microscopy List <[hidden email]> on behalf of Cromey, Douglas W - (dcromey) <[hidden email]>
> Sent: Wednesday, December 16, 2015 2:01 PM
> To: [hidden email]
> Subject: disinfecting a microscope
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> What is an appropriate method for disinfecting a microscope used for live cell imaging (or in a BSL2 facility)? 10% bleach is too strong, so what do people use? Thinking of core facility users (who can be poor at following directions), what is a relatively fool-proof way to decontaminate and not damage motorized stages, and other delicate parts?
>
> If you have posted protocols, can you send the links?  Do you have live cell imaging policies for your facility that you would be willing to share?
>
> Doug
>
> ------------------------------------------------------------------------------------------
> Douglas W. Cromey, M.S. - Associate Scientific Investigator
> Dept. of Cellular & Molecular Medicine, University of Arizona
> 1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA
>
> office:  UACC 0914A       email: [hidden email]<mailto:[hidden email]>
> voice:  520-626-2824       fax:  [phone]
>
> http://swehsc.pharmacy.arizona.edu/micro
> Home of: "Microscopy and Imaging Resources on the WWW"
>
> UA Microscopy Alliance -  http://microscopy.arizona.edu<http://microscopy.arizona.edu/>
>

--



George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
Tattletales http://works.bepress.com/gmcnamara/42
http://works.bepress.com/gmcnamara/75
https://www.linkedin.com/in/georgemcnamara

[Cromey, Douglas W - (dcromey)]

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To join, leave or search the confocal microscopy listserv, go to:
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*****

Thank you Dr. Periasamy.  Are there any others?  Does anyone have a BSL2 protocol already written up for a microscope core facility? I'd like not to re-invent the wheel and some of you have probably had to do this already.

Thanks.
Doug

------------------------------------------------------------------------------------------
Douglas W. Cromey, M.S. - Associate Scientific Investigator
Dept. of Cellular & Molecular Medicine, University of Arizona
1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA

office:  UACC 0914A       email: [hidden email]
voice:  520-626-2824       fax:  [phone]

http://swehsc.pharmacy.arizona.edu/micro
Home of: "Microscopy and Imaging Resources on the WWW"

UA Microscopy Alliance -  http://microscopy.arizona.edu


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Periasamy, Ammasi (ap3t)
Sent: Wednesday, December 16, 2015 12:13 PM
To: [hidden email]
Subject: Re: disinfecting a microscope

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Doug
This is what we use approved by our University Biosafety "Cavi Cide" you can spray on the microscopy stage and the desk area and wipe it.
Hope this helps.
Ammasi
Dr. Ammasi Periasamy
Professor & Center Director
http://www.kcci.virginia.edu/people/profile/ap3t
Phone: [phone] or 982-4869
Fax: [phone]
E-mail: [hidden email]
Office Location
W.M. Keck Center for Cellular Imaging
Physical and Life Sciences Building, (005) At the intersection of Geldard dr and White head Rd., Mailing or Shipping Address:
W.M. Keck Center for Cellular Imaging (PLSB 005) University of Virginia Biology, Gilmer Hall, 409 McCormick Rd.
Charlottesville, VA 22904, USA
FRET/FLIM Workshop-March 7-11, 2016: http://www.kcci.virginia.edu/workshop 


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Cromey, Douglas W - (dcromey)
Sent: Wednesday, December 16, 2015 2:02 PM
To: [hidden email]
Subject: disinfecting a microscope

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

What is an appropriate method for disinfecting a microscope used for live cell imaging (or in a BSL2 facility)? 10% bleach is too strong, so what do people use? Thinking of core facility users (who can be poor at following directions), what is a relatively fool-proof way to decontaminate and not damage motorized stages, and other delicate parts?

If you have posted protocols, can you send the links?  Do you have live cell imaging policies for your facility that you would be willing to share?

Doug

------------------------------------------------------------------------------------------
Douglas W. Cromey, M.S. - Associate Scientific Investigator Dept. of Cellular & Molecular Medicine, University of Arizona
1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA

office:  UACC 0914A       email: [hidden email]<mailto:[hidden email]>
voice:  520-626-2824       fax:  [phone]

http://swehsc.pharmacy.arizona.edu/micro
Home of: "Microscopy and Imaging Resources on the WWW"

UA Microscopy Alliance -  http://microscopy.arizona.edu<http://microscopy.arizona.edu/>

[Sathya]

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Hi Doug,
I was recently discussing about this issue to our Biosafety officers. What they say is: "Appropriate disinfection will depend on the nature of agent or the biohazard. A risk assessment will have to performed by the biosafety officer to determine appropriate disinfectant on a case by case basis. Assuming that all live samples will be sealed so the microscope technically should not come in contact with biohazardous material. So in general 70% Ethanol can be used as a general disinfectant. But, may have to consider other disinfectants (based on risk and agents) that are not corrosive to the microscope optics".

I was digging more on it on the Biosafety manual of the institute and came across - Quaternary ammonium compounds which are not recommended for spill clean-up, but may be utilized for surface decontamination. Manufacturer’s claims for newer formulations, like Coverage Plus NPD (http://www.steris.com/products/cleaners-and-disinfectants/coverage-plus-npd) and Roccal D-Plus (https://www.zoetisus.com/products/cats/roccal-d-plus.aspx), indicate that these are effective against most bacteria, viruses, and some fungi. They are relatively non-corrosive, so may be useful for surface disinfection of sensitive instruments. One thing that I am seeing that both Coverage Plus NPD and Roccal D-Plus comes with deodorant. No commercial interest. Good luck.


Sathya Srinivasan

________________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of Cromey, Douglas W - (dcromey) <[hidden email]>
Sent: December 17, 2015 4:19 PM
To: [hidden email]
Subject: Re: disinfecting a microscope

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Thank you Dr. Periasamy.  Are there any others?  Does anyone have a BSL2 protocol already written up for a microscope core facility? I'd like not to re-invent the wheel and some of you have probably had to do this already.

Thanks.
Doug

------------------------------------------------------------------------------------------
Douglas W. Cromey, M.S. - Associate Scientific Investigator
Dept. of Cellular & Molecular Medicine, University of Arizona
1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA

office:  UACC 0914A       email: [hidden email]
voice:  520-626-2824       fax:  [phone]

http://swehsc.pharmacy.arizona.edu/micro
Home of: "Microscopy and Imaging Resources on the WWW"

UA Microscopy Alliance -  http://microscopy.arizona.edu


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Periasamy, Ammasi (ap3t)
Sent: Wednesday, December 16, 2015 12:13 PM
To: [hidden email]
Subject: Re: disinfecting a microscope

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Doug
This is what we use approved by our University Biosafety "Cavi Cide" you can spray on the microscopy stage and the desk area and wipe it.
Hope this helps.
Ammasi
Dr. Ammasi Periasamy
Professor & Center Director
http://www.kcci.virginia.edu/people/profile/ap3t
Phone: [phone] or 982-4869
Fax: [phone]
E-mail: [hidden email]
Office Location
W.M. Keck Center for Cellular Imaging
Physical and Life Sciences Building, (005) At the intersection of Geldard dr and White head Rd., Mailing or Shipping Address:
W.M. Keck Center for Cellular Imaging (PLSB 005) University of Virginia Biology, Gilmer Hall, 409 McCormick Rd.
Charlottesville, VA 22904, USA
FRET/FLIM Workshop-March 7-11, 2016: http://www.kcci.virginia.edu/workshop


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Cromey, Douglas W - (dcromey)
Sent: Wednesday, December 16, 2015 2:02 PM
To: [hidden email]
Subject: disinfecting a microscope

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

What is an appropriate method for disinfecting a microscope used for live cell imaging (or in a BSL2 facility)? 10% bleach is too strong, so what do people use? Thinking of core facility users (who can be poor at following directions), what is a relatively fool-proof way to decontaminate and not damage motorized stages, and other delicate parts?

If you have posted protocols, can you send the links?  Do you have live cell imaging policies for your facility that you would be willing to share?

Doug

------------------------------------------------------------------------------------------
Douglas W. Cromey, M.S. - Associate Scientific Investigator Dept. of Cellular & Molecular Medicine, University of Arizona
1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA

office:  UACC 0914A       email: [hidden email]<mailto:[hidden email]>
voice:  520-626-2824       fax:  [phone]

http://swehsc.pharmacy.arizona.edu/micro
Home of: "Microscopy and Imaging Resources on the WWW"

UA Microscopy Alliance -  http://microscopy.arizona.edu<http://microscopy.arizona.edu/>

[mcammer]

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*****

Protocol may depend on the organisms it has been exposed to.  For instance, TB, hep-c, and HIV are very different beasts.  

=========================================================================
 Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical Center
                      Cell:  914-309-3270     ** Office: Skirball 2nd Floor main office, back right **
          http://ocs.med.nyu.edu/microscopy & http://microscopynotes.com/

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Cromey, Douglas W - (dcromey)
Sent: Thursday, December 17, 2015 11:20 AM
To: [hidden email]
Subject: Re: disinfecting a microscope

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Thank you Dr. Periasamy.  Are there any others?  Does anyone have a BSL2 protocol already written up for a microscope core facility? I'd like not to re-invent the wheel and some of you have probably had to do this already.

Thanks.
Doug

------------------------------------------------------------------------------------------
Douglas W. Cromey, M.S. - Associate Scientific Investigator Dept. of Cellular & Molecular Medicine, University of Arizona
1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA

office:  UACC 0914A       email: [hidden email]
voice:  520-626-2824       fax:  [phone]

http://swehsc.pharmacy.arizona.edu/micro
Home of: "Microscopy and Imaging Resources on the WWW"

UA Microscopy Alliance -  http://microscopy.arizona.edu


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Periasamy, Ammasi (ap3t)
Sent: Wednesday, December 16, 2015 12:13 PM
To: [hidden email]
Subject: Re: disinfecting a microscope

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Doug
This is what we use approved by our University Biosafety "Cavi Cide" you can spray on the microscopy stage and the desk area and wipe it.
Hope this helps.
Ammasi
Dr. Ammasi Periasamy
Professor & Center Director
http://www.kcci.virginia.edu/people/profile/ap3t
Phone: [phone] or 982-4869
Fax: [phone]
E-mail: [hidden email]
Office Location
W.M. Keck Center for Cellular Imaging
Physical and Life Sciences Building, (005) At the intersection of Geldard dr and White head Rd., Mailing or Shipping Address:
W.M. Keck Center for Cellular Imaging (PLSB 005) University of Virginia Biology, Gilmer Hall, 409 McCormick Rd.
Charlottesville, VA 22904, USA
FRET/FLIM Workshop-March 7-11, 2016: http://www.kcci.virginia.edu/workshop 


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Cromey, Douglas W - (dcromey)
Sent: Wednesday, December 16, 2015 2:02 PM
To: [hidden email]
Subject: disinfecting a microscope

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

What is an appropriate method for disinfecting a microscope used for live cell imaging (or in a BSL2 facility)? 10% bleach is too strong, so what do people use? Thinking of core facility users (who can be poor at following directions), what is a relatively fool-proof way to decontaminate and not damage motorized stages, and other delicate parts?

If you have posted protocols, can you send the links?  Do you have live cell imaging policies for your facility that you would be willing to share?

Doug

------------------------------------------------------------------------------------------
Douglas W. Cromey, M.S. - Associate Scientific Investigator Dept. of Cellular & Molecular Medicine, University of Arizona
1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA

office:  UACC 0914A       email: [hidden email]<mailto:[hidden email]>
voice:  520-626-2824       fax:  [phone]

http://swehsc.pharmacy.arizona.edu/micro
Home of: "Microscopy and Imaging Resources on the WWW"

UA Microscopy Alliance -  http://microscopy.arizona.edu<http://microscopy.arizona.edu/>

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[Eric Marino]

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*****

Good Morning Doug. This protocol of mine was approved by the Committee on Microbiological Safety at Harvard Medical School. Fortunately I never had to implement it.

https://dl.dropboxusercontent.com/u/3067386/BL2%20Spill%20Cleanup%20Rev%204.pages <https://dl.dropboxusercontent.com/u/3067386/BL2%20Spill%20Cleanup%20Rev%204.pages>


Eric Marino
[hidden email]

> On Dec 17, 2015, at 1:10 PM, Cammer, Michael <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Protocol may depend on the organisms it has been exposed to.  For instance, TB, hep-c, and HIV are very different beasts.  
>
> =========================================================================
> Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical Center
>                      Cell:  914-309-3270     ** Office: Skirball 2nd Floor main office, back right **
>          http://ocs.med.nyu.edu/microscopy & http://microscopynotes.com/
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Cromey, Douglas W - (dcromey)
> Sent: Thursday, December 17, 2015 11:20 AM
> To: [hidden email]
> Subject: Re: disinfecting a microscope
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Thank you Dr. Periasamy.  Are there any others?  Does anyone have a BSL2 protocol already written up for a microscope core facility? I'd like not to re-invent the wheel and some of you have probably had to do this already.
>
> Thanks.
> Doug
>
> ------------------------------------------------------------------------------------------
> Douglas W. Cromey, M.S. - Associate Scientific Investigator Dept. of Cellular & Molecular Medicine, University of Arizona
> 1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA
>
> office:  UACC 0914A       email: [hidden email]
> voice:  520-626-2824       fax:  [phone]
>
> http://swehsc.pharmacy.arizona.edu/micro
> Home of: "Microscopy and Imaging Resources on the WWW"
>
> UA Microscopy Alliance -  http://microscopy.arizona.edu
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>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Periasamy, Ammasi (ap3t)
> Sent: Wednesday, December 16, 2015 12:13 PM
> To: [hidden email]
> Subject: Re: disinfecting a microscope
>
> *****
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> Post images on http://www.imgur.com and include the link in your posting.
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>
> Doug
> This is what we use approved by our University Biosafety "Cavi Cide" you can spray on the microscopy stage and the desk area and wipe it.
> Hope this helps.
> Ammasi
> Dr. Ammasi Periasamy
> Professor & Center Director
> http://www.kcci.virginia.edu/people/profile/ap3t
> Phone: [phone] or 982-4869
> Fax: [phone]
> E-mail: [hidden email]
> Office Location
> W.M. Keck Center for Cellular Imaging
> Physical and Life Sciences Building, (005) At the intersection of Geldard dr and White head Rd., Mailing or Shipping Address:
> W.M. Keck Center for Cellular Imaging (PLSB 005) University of Virginia Biology, Gilmer Hall, 409 McCormick Rd.
> Charlottesville, VA 22904, USA
> FRET/FLIM Workshop-March 7-11, 2016: http://www.kcci.virginia.edu/workshop 
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>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Cromey, Douglas W - (dcromey)
> Sent: Wednesday, December 16, 2015 2:02 PM
> To: [hidden email]
> Subject: disinfecting a microscope
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> What is an appropriate method for disinfecting a microscope used for live cell imaging (or in a BSL2 facility)? 10% bleach is too strong, so what do people use? Thinking of core facility users (who can be poor at following directions), what is a relatively fool-proof way to decontaminate and not damage motorized stages, and other delicate parts?
>
> If you have posted protocols, can you send the links?  Do you have live cell imaging policies for your facility that you would be willing to share?
>
> Doug
>
> ------------------------------------------------------------------------------------------
> Douglas W. Cromey, M.S. - Associate Scientific Investigator Dept. of Cellular & Molecular Medicine, University of Arizona
> 1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA
>
> office:  UACC 0914A       email: [hidden email]<mailto:[hidden email]>
> voice:  520-626-2824       fax:  [phone]
>
> http://swehsc.pharmacy.arizona.edu/micro
> Home of: "Microscopy and Imaging Resources on the WWW"
>
> UA Microscopy Alliance -  http://microscopy.arizona.edu<http://microscopy.arizona.edu/>
>
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